A robust, brief measure of an individual's most preferred level of salt in an ordinary foodstuff

A single-session procedure to assess an individual's most preferred level of a factor in a product is justified theoretically and illustrated by the results for salt concentration in samples of bread and tomato soup tested on 30 young men who had had no previous experience of the task. Each man rated the saltiness of each sample as a distance below or above his ideal for that food type. Without the rater knowing, his stimulus set was coordinated to his rating responses in order to minimise biases in what others have shown can be a linear response mode. The Weber fraction is constant for the medium range of NaCl solutions when concentration units are used, and so Fechner's principle of direct scaling was applied: mean linear regressions between ideal-relative intensity responses and the logarithm of salt concentrations in each individual were nearly always statistically reliable with only six to 20 ratings of three to six salt levels in bread or soup. Values of the regression intercepts for bread at the initial session and five months later correlated significantly, as also did the regression slopes. Thus, a robust value for each individual's ideal salt level for each food could be interpolated from the regression equation. There was no effect of sequence of bread and soup sessions. Bread and soup salt-ideals were correlated, as were their slopes. A regression slope appears to represent an individual's tolerance of deviations from ideal. The relation of the slope to choice behaviour, and its relative dependence on intensity sensitivity and a preference motivation characteristic of the individual and test situation, remain to be elucidated. This procedure should have wide application in consumer preference measurement

Expression of Aurora kinases in human thyroid carcinoma cell lines and tissues

The Aurora kinases are involved in the regulation of cell cycle progression, and alterations in their expression have been shown to associate with cell malignant transformation. In the present study, we demonstrated that human thyrocytes express all 3 Aurora kinases (A, B and C) at both protein and mRNA level and this expression is cell cycle-regulated. An increase in the protein level of the 3 kinases was found, with respect to normal human thyrocytes (HTU5), in the human cell lines derived from follicular (FTC-133), papillary (B-CPAP) and anaplastic (8305C) thyroid carcinomas, but not in cells derived from a follicular adenoma (HTU42). These observations were mirrored in RT-PCR experiments for Aurora-A and B. In contrast, Aurora-C mRNA levels were not significantly different among the different cell types analyzed, suggesting that posttranscriptional mechanism(s) modulate its expression. The expression at the protein level of all 3 Aurora kinases was significantly higher in 3 thyroid papillary carcinomas with respect to normal matched tissues obtained from the same patients. Similar modifications, at the mRNA level, could be observed in 7 papillary carcinoma tissues for Aurora-A and B, but not for Aurora-C. In conclusion, we demonstrated that normal human thyrocytes express all 3 members of the Aurora kinase family, and their expression is amplified in malignant thyroid cell lines and tissues. These results suggest that the Aurora kinases may play a relevant role in malignant thyroid cancers, and may represent a putative therapeutic target for thyroid neoplasms. (c) 2006 Wiley-Liss, Inc