345 research outputs found
Managing Shiga Toxin-Producing E. coli Using Statistical Process Control Charts for Routine Health and Production Monitoring in Pig Farming
The peripartum period influenced the serum macromineral profile in mares
Changes in metabolic requirements during pregnancy may produce nutritional imbalances predisposing mares to pathological conditions. Therefore, we aimed to examine changes in serum macrominerals during the peripartum period in mares. Fifteen pregnant mares (Group A) were monitored starting at the 263rd day of pregnancy until the 21st day after foaling. Seven non-pregnant and non-lactating mares were used as a control group (Group B). Group A was subjected to blood sampling within 24-12 h after parturition (P) and then 7, 14 and 21 days after foaling (C1, C2, C3 weeks, respectively). Group B was subjected to regular sampling throughout the study to ensure that seasonal changes had no significant effect on studied parameters. Serum samples were analysed for serum electrolytes including sodium (NaC), potassium (KC), calcium (CaCC), phosphorus (PC), chloride (Cl) and magnesium (MgCC). Two-way repeated measures of analysis of variance (ANOVA) was applied to evaluate significant effects of peripartum on electrolyte concentrations. Group A showed a decrease in Cl concentrations (P D 0:011) after foaling and lower PC concentrations (P D 0:015) compared to Group B throughout the study. The linear regression model showed an increase in NaC concentrations (P D 0:002) and a gradual decrease in serum CaCC (P D 0:001) during pregnancy. Because the most significant changes in serum electrolytes occurred within 2 weeks before and 2 weeks after foaling, monitoring serum electrolyte concentrations would indicate whether the mare needs further mineral supplementation at this stage. The significant changes in a mare's macromineral profile that we observed over the peripartum provide enhanced knowledge about mineral interrelations and their modifications during specific physiological conditions such as late pregnancy and early postpartum
Tephra hazard assessment at Mt. Etna (Italy)
In this paper we present a probabilistic hazard assessment
for tephra fallout at Mt. Etna (Italy) associated with
both short- and long-lived eruptions. Eruptive scenarios and
eruption source parameters were defined based on the geological
record, while an advection–diffusion–sedimentation
model was used to capture the variation in wind speed and
direction with time after calibration with the field data. Two
different types of eruptions were considered in our analysis:
eruptions associated with strong short-lived plumes and eruptions
associated with weak long-lived plumes. Our probabilistic
approach was based on one eruption scenario for both
types and on an eruption range scenario for eruptions producing
weak long-lived plumes. Due to the prevailing wind direction,
the eastern flanks are the most affected by tephra deposition, with the 122 BC Plinian and 2002–2003 eruptions showing the highest impact both on infrastructures and agriculture
Analgesia and/or anaesthesia during piglet castration–part I: efficacy of farm protocols in pain management
Pain alleviation associated with surgical castration of piglets is a debated welfare issue. The present study compares the effect of different protocols involving analgesia and/or anaesthesia or sedation suitable under field conditions, with the aim to alleviate pain due to castration in piglets. A randomised within-litter design, with 50 replicates, compared 7 treatments applied 10 min before castration: HAND: positive control, handling only; CTRL: negative control, physiological saline, i.m.; MEL: meloxicam, i.m.; AZA: azaperone, i.m.; PROC: local anaesthesia with procaine and adrenaline, subcutaneously; AZA-MEL: joint administration of azaperone and meloxicam; PROC-MEL: procaine and meloxicam. Efficacy of pain relief was assessed during a 180 min period after castration by serum cortisol and glycaemia, algometry and behaviour. CTRL, AZA, PROC and AZA-MEL piglets showed an increase in cortisol concentration 60 min after castration compared to HAND. Both groups with azaperone (AZA and AZA-MEL) developed concentrations even higher than CTRL (p .05). CRTL and PROC piglets reacted to the algometer at an average lower pressure than HAND (p =.03), differently to the other treatments that showed similar skin sensitivity to HAND (p >.05). No differences in glycaemia and behaviour were observed among treatments. The results suggest that using meloxicam alone might offer a promising option in reducing the expression of pain-related parameters in piglets after surgical castration, however, it appears more efficient when used alone than in association with the anaesthetic agents tested. Procaine administered alone and azaperone seems unsuited to the purpose considered.HIGHLIGHTS Meloxicam used alone is suggested for reducing the expression of pain-related parameters in piglets after surgical castration; When a protocol using procaine is used on the farm during piglets castration, the association with meloxicam reduces some pain-related indicators; Procaine administered alone and azaperone seems unsuited to manage pain after surgical castration of piglets
Personal pack display and active smoking at outdoor café strips: assessing the impact of plain packaging 1 year postimplementation
Aims: We observed tobacco pack display and smoking at outdoor venues over three summers to assess changes in their prevalence following Australia's introduction of plain tobacco packaging with larger pictorial health warnings. Methods: Between January and April 2012 (preplain packaging (PP)), 2013 (early post-PP) and 2014 (1 year post-PP), we counted patrons, smokers and tobacco packs at cafés, restaurants and bars with outdoor seating. Pack type (branded, plain or unknown) and orientation were noted. Rates of active smoking, pack display and pack orientation were analysed using multilevel Poisson regression. Results: Prevalence of pack display among patrons declined from pre-PP (1 pack per 8.7 patrons) to early post-PP (1 pack per 10.4), and remained low 1 year post-PP (1 pack per 10.3). This appeared to be driven by a sustained decline in active smoking post-PP (pre-PP: 8.4% of patrons were smoking; early post-PP: 6.4%; 1 year post-PP: 6.8%). Notably, active smoking declined more in venues with children present than in those without. While early post-PP, plain packs were less often displayed face-up (74.0%) and more often concealed (8.9%) than branded packs pre-PP (face-up: 85.2%; concealed: 4.0%), this was not sustained 1 year post-PP (face-up: 85.7%; concealed: 4.4%). Also, external case use increased from pre-PP (1.2%) to early post-PP (3.5%), but returned to pre-PP levels 1 year post-PP (1.9%). Conclusions: This study demonstrated a sustained reduction in visibility of tobacco products and smoking in public, particularly in the presence of children, from pre-PP to 1 year post-PP. This effect is likely to reduce smoking-related social norms, thereby weakening an important influence on smoking uptake and better supporting quit attempts.Meghan Zacher, Megan Bayly, Emily Brennan, Joanne Dono, Caroline Miller, Sarah Durkin, Michelle Scollo, Melanie Wakefiel
Electrochemical Impedance Biosensor for Chagas Disease Diagnosis in Clinical Samples
Chagas disease (CD) is one of the main neglected tropical diseases, diagnosed mainly by serological tests performed in centralized laboratories, which severely limits the clinical management of the disease in communities with scarce resources. Herein, an electrochemical impedance biosensor for the detection of CD was developed for the first time using a cruzipain-based sensor surface. The protein, highly immunogenic and isolated from Trypanosoma cruzi, was immobilized over the surface of gold disc electrodes modified with 11-mercaptoundecanoic (MUA) and 6-mercapto-1-hexanol (MCH) self-assembled monolayers (SAMs). Reproducible sensor surfaces, yielding 38 ± 3% coverage as measured by Surface Plasmon Resonance, were obtained by amide coupling of 120 μg/mL cruzipain onto 1/10 MUA/MCH SAMs for 30 min. Under optimized operational conditions, the impedimetric immunosensor recognized specific interactions for anti-T. cruzi antibodies in 1/800 diluted human serum patient samples. The charge transfer resistance of the biosensors increases by ∼18% in the presence of the positive samples, whereas the negative samples give rise to a negligible increase of around 6%. An excellent selectivity to clinical samples from patients infected with T. cruzi was obtained. The clear signal difference obtained for positive and negative clinical samples highlights the applicability of the sensors for the point-of-care diagnosis of CD.</p
Exposure-based risk assessment and emergency management associated with the fallout of large clasts at Mount Etna
Fallout of ballistic blocks and bombs ejected from eruptive vents represents
a well-known hazard in areas
proximal to volcanoes (mostly <5 km from the vent). However, fallout of large clasts sedimenting from
plume margins that extend to medial
areas and have the potential to produce severe injuries to people and cause
damage to infrastructure, is often overlooked. Recent eruptive events at
Mount Etna (Italy) provide a clear example where large-clast fallout from
plume margins (>5 cm) has posed a real threat both to the many
visitors reaching the summit area and to local infrastructure, and,
therefore, has been selected as a case study. To quantify this hazard, a new
particle sedimentation model was calibrated with field data and then used for
probabilistic hazard assessments. For a fully probabilistic scenario the
hazard zone covered 72 km2 and included some 125 km of paths and
roads, as well as 15 buildings. Evacuation on foot to a safe area was
estimated at almost 4 h, but this could be reduced to less than 3 h if two
shelters were provided. Our results show the importance of integrating
probabilistic hazard analysis of large-clast fallout within effective
strategies of risk management and reduction, especially in the case of
volcanoes where visitors can reach the summit areas.</p
Development of molecular and antigenic-based rapid tests for the identification of African swine fever virus in different tissues
Examples of multi-sensor determination of eruptive source parameters of explosive events at mount etna
International audienceMulti-sensor strategies are key to the real-time determination of eruptive source parameters (ESPs) of explosive eruptions necessary to forecast accurately both tephra dispersal and deposition. To explore the capacity of these strategies in various eruptive conditions, we analyze data acquiredby two Doppler radars, ground- and satellite-based infrared sensors, one infrasound array, visible video-monitoring cameras as well as data from tephra-fallout deposits associated with a weak and a strong paroxysmal event at Mount Etna (Italy). We find that the different sensors provide complementary observations that should be critically analyzed and combined to provide comprehensive estimates of ESPs. First, all measurements of plume height agree during the strong paroxysmal activity considered, whereas some discrepancies are found for the weak paroxysm due to rapid plume and cloud dilution. Second, the event duration, key to convert the total erupted mass (TEM) in the mass eruption rate (MER) and vice versa, varies depending on the sensor used, providing information on different phases of the paroxysm (i.e., unsteady lava fountaining, lava fountain-fed tephra plume, waning phase associated with plume and cloud expansion in the atmosphere). As a result, TEM and MER derived from different sensors also correspond to the different phases of the paroxysms. Finally, satellite retrievals for grain-size can be combined with radar data to provide a first approximation of total grain-size distribution (TGSD) in near real-time. Such a TGSD shows a promising agreement with the TGSD derived from the combination of satellite data and whole deposit grain-size distribution (WDGSD)
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