Acoustic wood tomography on trees and the challenge of wood heterogeneity

The assessment of tree stability requires information about the location and the geometry of fungal decay or of a cavity in the interior of the trunk. This work aims at specifying which size of decay or cavity can be detected non-destructively by acoustic wood tomography. In the present work, the elastic waves that propagate in a trunk during a tomographic measurement were visualized by numerical simulations. The numerical model enabled to systematically investigate the influence of fungal decay on tomographic measurements neglecting the heterogeneity of wood. The influence of wood heterogeneity was studied in laboratory experiments on trunks. The experiments indicated that the waveforms of the measured signals are by far more sensitive to the natural heterogeneity of trunk wood than the travel times, thereby making waveforms unsuitable for decay detection. Thus, it is recommended to further develop the travel time inversion algorithms for trunks and to neglect the information in waveforms or amplitudes. Fungal decay is detectable if the influence of the decay is distinguishable from the influence of the heterogeneity. It was found from the numerical analysis that the cross-section of a cavity, which is larger than 5% of the total cross-section of the trunk, can be detected by acoustic wood tomograph

Rolling shear modulus and damping factor of spruce and decayed spruce estimated by modal analysis

Modal analysis was used to determine the rolling shear modulus of Norway spruce samples that were either untreated or inoculated with fungi. The resonance frequencies of centimeter-range cuboids were measured using contact-less laser interferometry. A three-dimensional theoretical model describing the orthotropic behavior of the material was used to calculate the resonance frequencies. Using an iterative scheme based on the least-squares method, the value of the rolling shear modulus was then extracted. In this first investigation, the decrease in the rolling shear modulus and the weight loss of Norway spruce inoculated with white-rot fungi Heterobasidion annosum and Ganoderma lipsiense were studied for three different exposure times ranging from 4 to 12weeks. Comparison of measured and theoretical resonance frequencies confirmed that operation was in the applicable range of the theoretical model for the inoculated specimens. A decrease in rolling shear modulus of up to 10% (H. annosum) and 50% (G. lipsiense) was foun

A simple anisotropy correction procedure for acoustic wood tomography

Anisotropy of acoustic propagation velocities is a ubiquitous feature of wood. This needs to be considered for successful application of travel time tomography, an increasingly popular technique for non-destructive testing of living trees. We have developed a simple correction scheme that removes first-order anisotropy effects. The corrected travel-time data can be inverted with isotropic inversion codes that are commercially available. Using a numerical experiment, we demonstrate the consequences of ignoring anisotropy effects and outline the performance of our correction scheme. The new technique has been applied to two spruce samples. Subsequent inspection of the samples revealed a good match with the tomogram

Growth mode, magnetic and magneto-optical properties of pulsed-laser-deposited Au/Co/Au(111) trilayers

The growth mode, magnetic and magneto-optical properties of epitaxial Au/Co/Au(111) ultrathin trilayers grown by pulsed-laser deposition (PLD) under ultra-high vacuum are presented. Sapphire wafers buffered with a single-crystalline Mo(110) bilayer were used as substrates. Owing to PLD-induced interfacial intermixing at the lower Co/Au(111) interface, a layer-by-layer growth mode is promoted. Surprisingly, despite this intermixing, ferromagnetic behavior is found at room temperature for coverings starting at 1 atomic layer (AL). The films display perpendicular magnetization with anisotropy constants reduced by 50% compared to TD-grown or electrodeposited films, and with a coercivity more than one order of magnitude lower ($\lesssim$ 5 mT). The magneto-optical (MO) response in the low Co thickness range is dominated by Au/Co interface contributions. For thicknesses starting at 3 AL Co, the MO response has a linear dependence with the Co thickness, indicative of a continuous-film-like MO behavior

Thermodynamic instability of siRNA duplex is a prerequisite for dependable prediction of siRNA activities

We developed a simple algorithm, i-Score (inhibitory-Score), to predict active siRNAs by applying a linear regression model to 2431 siRNAs. Our algorithm is exclusively comprised of nucleotide (nt) preferences at each position, and no other parameters are taken into account. Using a validation dataset comprised of 419 siRNAs, we found that the prediction accuracy of i-Score is as good as those of s-Biopredsi, ThermoComposition21 and DSIR, which employ a neural network model or more parameters in a linear regression model. Reynolds and Katoh also predict active siRNAs efficiently, but the numbers of siRNAs predicted to be active are less than one-eighth of that of i-Score. We additionally found that exclusion of thermostable siRNAs, whose whole stacking energy (ΔG) is less than −34.6 kcal/mol, improves the prediction accuracy in i-Score, s-Biopredsi, ThermoComposition21 and DSIR. We also developed a universal target vector, pSELL, with which we can assay an siRNA activity of any sequence in either the sense or antisense direction. We assayed 86 siRNAs in HEK293 cells using pSELL, and validated applicability of i-Score and the whole ΔG value in designing siRNAs

Acoustic wood tomography on trees and the challange of wood heterogenity

ISSN:0018-3830ISSN:1437-434

A simple anisotropy correction procedure for acoustic wood tomography

ISSN:0018-3830ISSN:1437-434