Social Referencing in the Domestic Horse

Dogs and cats use human emotional information directed to an unfamiliar situation to guide their behavior, known as social referencing. It is not clear whether other domestic species show similar socio-cognitive abilities in interacting with humans. We investigated whether horses (n = 46) use human emotional information to adjust their behavior to a novel object and whether the behavior of horses differed depending on breed type. Horses were randomly assigned to one of two groups: an experimenter positioned in the middle of a test arena directed gaze and voice towards the novel object with either (a) a positive or (b) a negative emotional expression. The duration of subjects’ position to the experimenter and the object in the arena, frequency of gazing behavior, and physical interactions (with either object or experimenter) were analyzed. Horses in the positive condition spent more time between the experimenter and object compared to horses in the negative condition, indicating less avoidance behavior towards the object. Horses in the negative condition gazed more often towards the object than horses in the positive condition, indicating increased vigilance behavior. Breed types differed in their behavior: thoroughbreds showed less human-directed behavior than warmbloods and ponies. Our results provide evidence that horses use emotional cues from humans to guide their behavior towards novel objects

Weight-related stigmatization and its impact on behavioral adaptations, affect, and parasympathetic activity during social information processing – a cross-cultural comparison

The aim of this thesis is to provide insights into how previous negative social experiences and/or societal norms concerning body size influence social information processing. The first two studies included in this thesis were conducted in Germany, a society with high level of prejudice towards individuals with obesity. The third study was conducted in American Samoa, a society with low prejudice towards individuals with obesity. Study one (chapter 5.1) investigated potential alterations in social information processing and behavior in individuals with obesity compared to lean individuals by measuring heart rate and reaction times. To identify potential reasons for alterations in the individual’s personal history, the influence of weight-related teasing on social information processing was analyzed. In the second study (chapter 5.2), I examined how individuals with and without obesity respond to social inclusion and social exclusion by measuring affect and heart rate. Of special interest were subjective factors of body dissatisfaction and teasing experiences as potential influencing factors. Following this approach, the second study became a “travelling experiment” and was repeated in a Polynesian group on American Samoa (chapter 5.3), a country with a high obesity rate, but less negative social outcomes for individuals with obesity. It aimed at studying the influence of cultural norms and values related to body size as well as varying weight-related stigmatization on psychophysiological processing in social situations.:1 Introduction 2 Theoretical Background 2.1 Social exclusion and behavior 2.1.1 Evolutionary origins of social exclusion and stigmatization 2.1.2 Adaptive behavioral responses to recurrent negative social feedback 2.2 Social exclusion and affect 2.2.1 General biological aspects of emotions 2.2.2 Cultural aspects of emotions 2.2.3 Affect after social exclusion 2.3 Social exclusion and parasympathetic cardio-regulation 2.3.1 Parasympathetic cardio-regulation in psychological research 2.3.2 Parasympathetic cardio-regulation during negative social feedback 2.3.3 Methodological issues 2.4 Obesity in Western societies 2.4.1 Prevalence and health consequences 2.4.2 Obesity and parasympathetic cardio-regulation 2.4.3 The stigma of obesity 2.4.4 Obesity and social information processing 2.5 Obesity and the Samoan Islands 2.5.1 Geography and population 2.5.2 Traditional diets, modernization, and obesity prevalence 2.5.3 Cultural differences in the perception of body sizes 2.5.4 Body size perception in the Samoan Islands 2.5.5 Affect in Samoa 3 Rationale of the Experimental Work 4 Methods 4.1 Autonomic control of the heart 4.2 Heart rate variability analyses 5 Experimental Work 5.1 Study 1 – Differential heart rate responses to social and monetary reinforcement in women with obesity 5.2 Study 2 – Parasympathetic cardio-regulation during social interactions in individuals with obesity – The influence of negative body image 5.3 Study 3 – Body size-related socio-cultural norms in Westerners and Polynesians and their association with heart rate variability and affect during social interaction 5.3.1 Introduction 5.3.2 Methods 5.3.3 Results 5.3.4 Discussion 6 General Discussion and Implications 7 Summary 7.1 English summary 7.2 German summary 8 References 9 Appendix 9.1 Supplemental material 9.1.1 Participants: recruitment, inclusion and exclusion criteria 9.1.2 Overview procedures in Germany and American Samoa 9.1.3 Study 1 9.1.4 Study 2 9.1.5 Study 3 9.2 Declaration of authenticity 9.3 Authors' contributions 9.3.1 Study 1 9.3.2 Study 2 9.3.3 Study

The Impact of the COVID-19 Pandemic on Avoidance of Health Care, Symptom Severity, and Mental Well-Being in Patients With Coronary Artery Disease

The COVID-19 pandemic affected regular health care for patients with chronic diseases. However, the impact of the pandemic on primary care for patients with coronary artery disease (CAD) who are enrolled in a structured disease management program (DMP) in Germany is not clear. We investigated whether the pandemic affected primary care and health outcomes of DMP-CAD patients (n = 750) by using a questionnaire assessing patients’ utilization of medical care, CAD symptoms, as well as health behavior and mental health since March 2020. We found that out of concern about getting infected with COVID-19, 9.1% of the patients did not consult a medical practitioner despite having CAD symptoms. Perceived own influence on infection risk was lower and anxiety was higher in these patients compared to symptomatic CAD patients who consulted a physician. Among the patients who reported chest pain lasting longer than 30 min, one third did not consult a medical practitioner subsequently. These patients were generally more worried about COVID-19. Patients with at least one worsening CAD symptom (chest pain, dyspnea, perspiration, or nausea without apparent reason) since the pandemic showed more depressive symptoms, higher anxiety scores, and were less likely to consult a doctor despite having CAD symptoms out of fear of infection. Our results provide evidence that the majority of patients received sufficient medical care during the COVID-19 pandemic in Germany. However, one in ten patients could be considered particularly at risk for medical undersupply and adverse health outcomes. The perceived infection risk with COVID-19 might have facilitated the decision not to consult a medical doctor

A journey on plate tectonics sheds light on European crayfish phylogeography

Abstract Crayfish can be used as model organisms in phylogeographic and divergence time studies if reliable calibrations are available. This study presents a comprehensive investigation into the phylogeography of the European stone crayfish (Austropotamobius torrentium) and includes samples from previously unstudied sites. Two mitochondrial markers were used to reveal evolutionary relationships among haplogroups throughout the species? distributional range and to estimate the divergence time by employing both substitution rates and geological calibration methods. Our haplotype network reconstruction and phylogenetic analyses revealed the existence of a previously unknown haplogroup distributed in Romania's Apuseni Mountains. This haplogroup is closely related to others that are endemic in the Dinarides, despite their vast geographical separation (~600 km). The separation is best explained by the well-dated tectonic displacement of the Tisza?Dacia microplate, which started in the Miocene (~16 Ma) and possibly carried part of the A. torrentium population to the current location of the Apuseni Mountains. This population may thus have been isolated from the Dinarides for a period of ca. 11 m.y. by marine and lacustrine phases of the Pannonian Basin. The inclusion of this geological event as a calibration point in divergence time analyses challenges currently accepted crayfish evolutionary time frames for the region, constraining the evolution of this area's crayfish to a much earlier date. We discuss why molecular clock calibrations previously employed to date European crayfish species divergences should therefore be reconsidered

Hemispheric asymmetries in resting-state EEG and fMRI are related to approach and avoidance behaviour, but not to eating behaviour or BMI

Much of our behaviour is driven by two motivational dimensions-approach and avoidance. These have been related to frontal hemispheric asymmetries in clinical and resting-state EEG studies: Approach was linked to higher activity of the left relative to the right hemisphere, while avoidance was related to the opposite pattern. Increased approach behaviour, specifically towards unhealthy foods, is also observed in obesity and has been linked to asymmetry in the framework of the right-brain hypothesis of obesity. Here, we aimed to replicate previous EEG findings of hemispheric asymmetries for self-reported approach/avoidance behaviour and to relate them to eating behaviour. Further, we assessed whether resting fMRI hemispheric asymmetries can be detected and whether they are related to approach/avoidance, eating behaviour and BMI. We analysed three samples: Sample 1 (n = 117) containing EEG and fMRI data from lean participants, and Samples 2 (n = 89) and 3 (n = 152) containing fMRI data from lean, overweight and obese participants. In Sample 1, approach behaviour in women was related to EEG, but not to fMRI hemispheric asymmetries. In Sample 2, approach/avoidance behaviours were related to fMRI hemispheric asymmetries. Finally, hemispheric asymmetries were not related to either BMI or eating behaviour in any of the samples. Our study partly replicates previous EEG findings regarding hemispheric asymmetries and indicates that this relationship could also be captured using fMRI. Our findings suggest that eating behaviour and obesity are likely to be mediated by mechanisms not directly relating to frontal asymmetries in neuronal activation quantified with EEG and fMRI.Peer reviewe

DNA methylation-based classification of central nervous system tumours.

Accurate pathological diagnosis is crucial for optimal management of patients with cancer. For the approximately 100 known tumour types of the central nervous system, standardization of the diagnostic process has been shown to be particularly challenging-with substantial inter-observer variability in the histopathological diagnosis of many tumour types. Here we present a comprehensive approach for the DNA methylation-based classification of central nervous system tumours across all entities and age groups, and demonstrate its application in a routine diagnostic setting. We show that the availability of this method may have a substantial impact on diagnostic precision compared to standard methods, resulting in a change of diagnosis in up to 12% of prospective cases. For broader accessibility, we have designed a free online classifier tool, the use of which does not require any additional onsite data processing. Our results provide a blueprint for the generation of machine-learning-based tumour classifiers across other cancer entities, with the potential to fundamentally transform tumour pathology

FcγR-mediated SARS-CoV-2 infection of monocytes activates inflammation

SARS-CoV-2 can cause acute respiratory distress and death in some patients1. Although severe COVID-19 disease is linked to exuberant inflammation, how SARS-CoV-2 triggers inflammation is not understood2. Monocytes and macrophages are sentinel cells that sense invasive infection to form inflammasomes that activate caspase-1 and gasdermin D (GSDMD), leading to inflammatory death (pyroptosis) and release of potent inflammatory mediators3. Here we show that about 6% of blood monocytes in COVID-19 patients are infected with SARS-CoV-2. Monocyte infection depends on uptake of antibody-opsonized virus by Fcγ receptors. Vaccine recipient plasma does not promote antibody-dependent monocyte infection. SARS-CoV-2 begins to replicate in monocytes, but infection is aborted, and infectious virus is not detected in infected monocyte culture supernatants. Instead, infected cells undergo inflammatory cell death (pyroptosis) mediated by activation of NLRP3 and AIM2 inflammasomes, caspase-1 and GSDMD. Moreover, tissue-resident macrophages, but not infected epithelial and endothelial cells, from COVID-19 lung autopsies have activated inflammasomes. These findings taken together suggest that antibody-mediated SARS-CoV-2 uptake by monocytes/macrophages triggers inflammatory cell death that aborts production of infectious virus but causes systemic inflammation that contributes to COVID-19 pathogenesis

What is damaging the kidney in lupus nephritis?

Despite marked improvements in the survival of patients with severe lupus nephritis over the past 50 years, the rate of complete clinical remission after immune suppression therapy i

Social Referencing in the Domestic Horse

Dogs and cats use human emotional information directed to an unfamiliar situation to guide their behavior, known as social referencing. It is not clear whether other domestic species show similar socio-cognitive abilities in interacting with humans. We investigated whether horses (n = 46) use human emotional information to adjust their behavior to a novel object and whether the behavior of horses differed depending on breed type. Horses were randomly assigned to one of two groups: an experimenter positioned in the middle of a test arena directed gaze and voice towards the novel object with either (a) a positive or (b) a negative emotional expression. The duration of subjects’ position to the experimenter and the object in the arena, frequency of gazing behavior, and physical interactions (with either object or experimenter) were analyzed. Horses in the positive condition spent more time between the experimenter and object compared to horses in the negative condition, indicating less avoidance behavior towards the object. Horses in the negative condition gazed more often towards the object than horses in the positive condition, indicating increased vigilance behavior. Breed types differed in their behavior: thoroughbreds showed less human-directed behavior than warmbloods and ponies. Our results provide evidence that horses use emotional cues from humans to guide their behavior towards novel objects

Weight-related stigmatization and its impact on behavioral adaptations, affect, and parasympathetic activity during social information processing – a cross-cultural comparison

The aim of this thesis is to provide insights into how previous negative social experiences and/or societal norms concerning body size influence social information processing. The first two studies included in this thesis were conducted in Germany, a society with high level of prejudice towards individuals with obesity. The third study was conducted in American Samoa, a society with low prejudice towards individuals with obesity. Study one (chapter 5.1) investigated potential alterations in social information processing and behavior in individuals with obesity compared to lean individuals by measuring heart rate and reaction times. To identify potential reasons for alterations in the individual’s personal history, the influence of weight-related teasing on social information processing was analyzed. In the second study (chapter 5.2), I examined how individuals with and without obesity respond to social inclusion and social exclusion by measuring affect and heart rate. Of special interest were subjective factors of body dissatisfaction and teasing experiences as potential influencing factors. Following this approach, the second study became a “travelling experiment” and was repeated in a Polynesian group on American Samoa (chapter 5.3), a country with a high obesity rate, but less negative social outcomes for individuals with obesity. It aimed at studying the influence of cultural norms and values related to body size as well as varying weight-related stigmatization on psychophysiological processing in social situations.:1 Introduction 2 Theoretical Background 2.1 Social exclusion and behavior 2.1.1 Evolutionary origins of social exclusion and stigmatization 2.1.2 Adaptive behavioral responses to recurrent negative social feedback 2.2 Social exclusion and affect 2.2.1 General biological aspects of emotions 2.2.2 Cultural aspects of emotions 2.2.3 Affect after social exclusion 2.3 Social exclusion and parasympathetic cardio-regulation 2.3.1 Parasympathetic cardio-regulation in psychological research 2.3.2 Parasympathetic cardio-regulation during negative social feedback 2.3.3 Methodological issues 2.4 Obesity in Western societies 2.4.1 Prevalence and health consequences 2.4.2 Obesity and parasympathetic cardio-regulation 2.4.3 The stigma of obesity 2.4.4 Obesity and social information processing 2.5 Obesity and the Samoan Islands 2.5.1 Geography and population 2.5.2 Traditional diets, modernization, and obesity prevalence 2.5.3 Cultural differences in the perception of body sizes 2.5.4 Body size perception in the Samoan Islands 2.5.5 Affect in Samoa 3 Rationale of the Experimental Work 4 Methods 4.1 Autonomic control of the heart 4.2 Heart rate variability analyses 5 Experimental Work 5.1 Study 1 – Differential heart rate responses to social and monetary reinforcement in women with obesity 5.2 Study 2 – Parasympathetic cardio-regulation during social interactions in individuals with obesity – The influence of negative body image 5.3 Study 3 – Body size-related socio-cultural norms in Westerners and Polynesians and their association with heart rate variability and affect during social interaction 5.3.1 Introduction 5.3.2 Methods 5.3.3 Results 5.3.4 Discussion 6 General Discussion and Implications 7 Summary 7.1 English summary 7.2 German summary 8 References 9 Appendix 9.1 Supplemental material 9.1.1 Participants: recruitment, inclusion and exclusion criteria 9.1.2 Overview procedures in Germany and American Samoa 9.1.3 Study 1 9.1.4 Study 2 9.1.5 Study 3 9.2 Declaration of authenticity 9.3 Authors' contributions 9.3.1 Study 1 9.3.2 Study 2 9.3.3 Study