Emulsions stabilised by whey protein microgel particles: Towards food-grade Pickering emulsions

We have investigated a new class of food-grade particles, whey protein microgels, as stabilisers of triglyceride-water emulsions. The sub-micron particles stabilized oil-in-water emulsions at all pH with and without salt. All emulsions creamed but exhibited exceptional resistance to coalescence. Clear correlations exist between the properties of the microgels in aqueous dispersion and the resulting emulsion characteristics. For conditions in which the particles were uncharged, fluid emulsions with relatively large drops were stabilised, whereas emulsions stabilized by charged particles contained smaller flocculated drops. A combination of optical microscopy of the drops and spectrophotometry of the resolved aqueous phase allowed us to estimate the interfacial adsorption densities of the particles using the phenomenon of limited coalescence. We deduce two classes of particle arrangement. Complete adsorption of the particles was obtained when they were neutral or when their charges were screened by salt resulting in at least one particle monolayer at the interface. By contrast, only around 50% of the particles adsorbed when they were charged with emulsion drops being covered by less than half a monolayer. These findings were supported by direct visualization of drop interfaces using cryo-scanning electron microscopy. Uncharged particles were highly aggregated and formed a continuous 2-D network at the interface. Otherwise particles organized as individual aggregates separated by particle-free regions. In this case, we suggest that some particles spread at the interface leading to the formation of a continuous protein membrane. Charged particles displayed the ability to bridge opposing interfaces of neighbouring drops to form dense particle disks protecting drops against coalescence; this is the main reason for the flocculation and stability of emulsions containing sparsely covered drops. © 2014 the Partner Organisations

Modulation de l’expression et de la fonction des protéines dopaminergiques présynaptiques par les statines : Application potentielle pour une intervention thérapeutique dans la maladie de Parkinson.

Parkinson disease (PD) is characterized by a progressive loss of dopaminergic presynaptic terminals and remains incurable. However in epidemiological studies, it has been shown that the use of statins, which are hypocholesterolemic drugs, diminishes the risk to develop a PD. Statins are able to inhibit the neurodegenerative effects in in-vitro and in-vivo models of PD. However, the molecular mechanisms driving neuroprotective effects are not yet fully understood. Consequently, we investigated the potential effects of statins on the synaptic expression and dopamine transport function in the dopaminergic system. In our studies, statins enhance the neurite outgrowth in the dopaminergic cells and trigger an increase in the expression levels of presynaptic dopaminergic proteins such as vesicular monoamine transporter 2 (VMAT2) and dopamine transporter. Statins induce a reduction of dopamine cellular uptake and modulate the binding-affinity of the specific inhibitors for VMAT2. The activation of the nuclear transcriptional factor sterol regulatory element-binding protein 1 (SREBP-1), cholesterol-dependent, could be the key element of the overexpression of dopaminergic presynaptic markers induced by the statins. Furthermore, these findings highlight the therapeutic neuroprotective and/or neurorestorative potentials of statins previously proposed in PD and allow to bring out new potential therapeutic targets such as SREBP factor.La maladie de Parkinson (MP) est caractérisée par une perte progressive des terminaisons présynaptiques dopaminergiques et reste actuellement incurable. Néanmoins, dans les études épidémiologiques, il a été montré que l’utilisation des statines, médicaments hypocholestérolémiants, diminue le risque de développer une MP. Les statines sont également capables d'inhiber les effets neurodégénératifs dans les modèles précliniques in-vitro et in-vivo de la MP. Cependant, les mécanismes moléculaires à l’origine de ces effets neuroprotecteurs ne sont pas encore complétement élucidés. Ainsi, nous avons étudié les effets potentiels des statines sur l'expression des marqueurs synaptiques et sur le transport de la dopamine. Dans nos études, les statines induisent la croissance des neurites dans les cellules dopaminergiques et déclenchent une augmentation de l’expression des protéines synaptiques dopaminergiques telles que le transporteur vésiculaire des monoamines (VMAT2) et le transporteur de la dopamine. Les statines induisent une diminution de la recapture de la dopamine cellulaire et des changements d’affinités aux niveaux des sites de liaison des inhibiteurs sélectifs du VMAT2. L’activation du facteur de transcription nucléaire protéine-1 se liant à l'élément de régulation des stérols (SREBP-1), cholestérol-dépendent, serait l’élément inducteur de la surexpression des marqueurs dopaminergiques présynaptiques induite par les statines. En outre, ces résultats soutiennent un potentiel thérapeutique neuroprotecteur et/ou neurorestaurateur des statines précédemment proposées dans la MP et permettent de mettre en évidence de nouvelles cibles thérapeutiques comme le facteur SREBP

Steinberg-like theorems for backbone colouring

International audienceA function f:V(G)→{1,…,k}f:V(G)→{1,…,k} is a (proper) k-colouring of G if |f(u)−f(v)|≥1|f(u)−f(v)|≥1, for every edge uv∈E(G)uv∈E(G). The chromatic number χ(G)χ(G) is the smallest integer k for which there exists a proper k-colouring of G.Given a graph G and a subgraph H of G, a circular q-backbone k-colouring c of (G, H) is a k-colouring of G such that q≤|c(u)−c(v)|≤k−qq≤|c(u)−c(v)|≤k−q, for each edge uv∈E(H)uv∈E(H). The circular q-backbone chromatic number of a graph pair (G, H ), denoted CBCq(G,H)CBCq(G,H), is the minimum k such that (G, H) admits a circular q-backbone k-colouring.In this work, we first show that if G is a planar graph containing no cycle on 4 or 5 vertices and H⊆GH⊆G is a forest, then CBC2(G,H)≤7CBC2(G,H)≤7. Then, we prove that if H⊆GH⊆G is a forest whose connected components are paths, then CBC2(G,H)≤6CBC2(G,H)≤6

Steinberg-like theorems for backbone colouring

Une fonction $f: V(G)\to \{1,\ldots,k\}$ est une $k$-coloration (propre) de $G$ si $|f (u) - f (v)|\geq 1$, pour toute ar\^ete $uv\in E(G)$. Le {\it nombre chromatique} $\chi(G)$ est le plus petit entier $k$ tel qu'il existe une $k$-coloration propre de $G$.Etant donn\'es un graphe $G$ et un sous-graphe $H$ de $G$, une $k$-coloration $q$-backbone circulaire $f$ de $(G,H)$ est une $k$-coloration de $G$ telle que $q\leq |c(u)-c(v)|\leq k-q$, pour tout ar\^ete $uv\in E(H)$. Le {\it nombre chromatique $q$-backbone circulaire} d'une paire de graphes $(G,H)$, not\'e \CBC_q(G,H), est le plus petit $k$ tel que $(G,H)$ admette une $k$-coloration $q$-backbone circulaire.Steinberg a conjectur\'e que si $G$ est planaire et si $G$ ne contient pas de cycles \`a 4 ou 5 sommets, alors $\chi(G)\leq 3$. tSi cette conjecture est correcte, alors on pourrait en d\'eduire que \CBC_2(G,H)\leq 6, pour tout $H\subseteq G$. Dans ce papier, nous montrons que si $G$ est un graphe planaire sans cycle \`a 4 ou 5 sommets et $H\subseteq G$ est une for\^et, alors \CBC_2(G,H)\leq 7. Ensuite, nous prouvons que si $H\subseteq G$ est une for\^et dont toutes les composantes connexes sont des chemins, alors \CBC_2(G,H)\leq 6

New near-IR observations of mesospheric CO2 and H2O clouds on Mars

Carbon dioxide clouds, which are speculated by models on solar and extra-solar planets, have been recently observed near the equator of Mars. The most comprehensive identification of Martian CO2 ice clouds has been obtained by the near-IR imaging spectrometer OMEGA. CRISM, a similar instrument with a higher spatial resolution, cannot detect these clouds with the same method due to its shorter wavelength range. Here we present a new method to detect CO2 clouds using near-IR data based on the comparison of H2O and CO2 ice spectral properties. The spatial and seasonal distributions of 54 CRISM observations containing CO2 clouds are reported, in addition to 17 new OMEGA observations. CRISM CO2 clouds are characterized by grain size in the 0.5-2\mum range and optical depths lower than 0.3. The distributions of CO2 clouds inferred from OMEGA and CRISM are consistent with each other and match at first order the distribution of high altitude (>60km) clouds derived from previous studies. At second order, discrepancies are observed. We report the identification of H2O clouds extending up to 80 km altitude, which could explain part of these discrepancies: both CO2 and H2O clouds can exist at high, mesospheric altitudes. CRISM observations of afternoon CO2 clouds display morphologies resembling terrestrial cirrus, which generalizes a previous result to the whole equatorial clouds season. Finally, we show that morning OMEGA observations have been previously misinterpreted as evidence for cumuliform, and hence potentially convective, CO2 clouds.Comment: Vincendon, M., C. Pilorget, B. Gondet, S. Murchie, and J.-P. Bibring (2011), New near-IR observations of mesospheric CO2 and H2O clouds on Mars, J. Geophys. Res., 116, E00J0

Sawhorse-type diruthenium tetracarbonyl complexes containing porphyrin-derived ligands as highly selective photosensitizers for female reproductive cancer cells

Diruthenium tetracarbonyl complexes of the type [Ru2(CO)4(μ2-η2-O2CR)2L2] containing a Ru-Ru backbone with four equatorial carbonyl ligands, two carboxylato bridges, and two axial two-electron ligands in a sawhorse-like geometry have been synthesized with porphyrin-derived substituents in the axial ligands [1: R is CH3, L is 5-(4-pyridyl)-10,15,20-triphenyl-21,23H-porphyrin], in the bridging carboxylato ligands [2: RCO2H is 5-(4-carboxyphenyl)-10,15,20-triphenyl-21,23H-porphyrin, L is PPh3; 3: RCO2H is 5-(4-carboxyphenyl)-10,15,20-triphenyl-21,23H-porphyrin, L is 1,3,5-triaza-7-phosphatricyclo[3.3.1.1]decane], or in both positions [4: RCO2H is 5-(4-carboxyphenyl)-10,15,20-triphenyl-21,23H-porphyrin, L is 5-(4-pyridyl)-10,15,20-triphenyl-21,23H-porphyrin]. Compounds 1-3 were assessed on different types of human cancer cells and normal cells. Their uptake by cells was quantified by fluorescence and checked by fluorescence microscopy. These compounds were taken up by human HeLa cervix and A2780 and Ovcar ovarian carcinoma cells but not by normal cells and other cancer cell lines (A549 pulmonary, Me300 melanoma, PC3 and LnCap prostate, KB head and neck, MDAMB231 and MCF7 breast, or HT29 colon cancer cells). The compounds demonstrated no cytotoxicity in the absence of laser irradiation but exhibited good phototoxicities in HeLa and A2780 cells when exposed to laser light at 652nm, displaying an LD50 between 1.5 and 6.5J/cm2 in these two cell lines and more than 15J/cm2 for the others. Thus, these types of porphyric compound present specificity for cancer cell lines of the female reproductive system and not for normal cells; thus being promising new organometallic photosensitizer

Modulation of Metabolism and Switching to Biofilm Prevail over Exopolysaccharide Production in the Response of Rhizobium alamii to Cadmium

Heavy metals such as cadmium (Cd2+) affect microbial metabolic processes. Consequently, bacteria adapt by adjusting their cellular machinery. We have investigated the dose-dependent growth effects of Cd2+ on Rhizobium alamii, an exopolysaccharide (EPS)-producing bacterium that forms a biofilm on plant roots. Adsorption isotherms show that the EPS of R. alamii binds cadmium in competition with calcium. A metabonomics approach based on ion cyclotron resonance Fourier transform mass spectrometry has showed that cadmium alters mainly the bacterial metabolism in pathways implying sugars, purine, phosphate, calcium signalling and cell respiration. We determined the influence of EPS on the bacterium response to cadmium, using a mutant of R. alamii impaired in EPS production (MSΔGT). Cadmium dose-dependent effects on the bacterial growth were not significantly different between the R. alamii wild type (wt) and MSΔGT strains. Although cadmium did not modify the quantity of EPS isolated from R. alamii, it triggered the formation of biofilm vs planktonic cells, both by R. alamii wt and by MSΔGT. Thus, it appears that cadmium toxicity could be managed by switching to a biofilm way of life, rather than producing EPS. We conclude that modulations of the bacterial metabolism and switching to biofilms prevails in the adaptation of R. alamii to cadmium. These results are original with regard to the conventional role attributed to EPS in a biofilm matrix, and the bacterial response to cadmium

Near-tropical subsurface ice on Mars

Near-surface perennial water ice on Mars has been previously inferred down to latitudes of about 45{\deg} and could result from either water vapor diffusion through the regolith under current conditions or previous ice ages precipitations. In this paper we show that at latitudes as low as 25{\deg} in the southern hemisphere buried water ice in the shallow (< 1 m) subsurface is required to explain the observed surface distribution of seasonal CO2 frost on pole facing slopes. This result shows that possible remnants of the last ice age, as well as water that will be needed for the future exploration of Mars, are accessible significantly closer to the equator than previously thought, where mild conditions for both robotic and human exploration lie

A large X-ray flare from the Herbig Ae star V892 Tau

We report the XMM-Newton observation of a large X-ray flare from the Herbig Ae star V892 Tau. The apparent low mass companion of V892 Tau, V892 Tau NE, is unresolved by XMM-Newton. Nevertheless there is compelling evidence from combined XMM-Newton and Chandra data that the origin of the flare is the Herbig Ae star V892 Tau. During the flare the X-ray luminosity of V892 Tau increases by a factor of ~15, while the temperature of the plasma increases from kT ~ 1.5 keV to kT ~ 8 keV. From the scaling of the flare event, based on hydrodynamic modeling, we conclude that a 500 G magnetic field is needed in order to confine the plasma. Under the assumptions that a dynamo mechanism is required to generate such a confining magnetic field and that surface convection is a necessary ingredient for a dynamo, our findings provide indirect evidence for the existence of a significant convection zone in the stellar envelope of Herbig Ae stars.Comment: accepted for publication in A&A (12 pages - 6 figures

Water ice at low to midlatitudes on Mars

In this paper, we analyze water ice occurrences at the surface of Mars using near-infrared observations, and we study their distribution with a climate model. Latitudes between 45{\deg}S and 50{\deg}N are considered. Data from the Observatoire pour la Min\'eralogie, l'Eau, les Glaces et l'Actitit\'e and the Compact Reconnaissance Imaging Spectrometer for Mars are used to assess the presence of surface water ice as a function of location and season. A modeling approach combining the 1-D and 3-D versions of the General Circulation Model of the Laboratoire de M\'et\'eorologie Dynamique de Jussieu is developed and successfully compared to observations. Ice deposits 2-200 \mu m thick are observed during the day on pole facing slopes in local fall, winter and early spring. Ice extends down to 13{\deg} latitude in the Southern Hemisphere but is restricted to latitudes higher than 32{\deg} in the north. On a given slope, the pattern of ice observations at the surface is mainly controlled by the global variability of atmospheric water (precipitation and vapor), with local ground properties playing a lower role. Only seasonal surface ice is observed: no exposed patches of perennial ground ice have been detected. Surface seasonal ice is however sensitive to subsurface properties: the results presented in this study are consistent with the recent discovery of low latitude subsurface ice obtained through the analysis of CO2 frost