Draft genome sequence of Agrobacterium deltaense strain CNPSo 3391, isolated from a soybean nodule in Mozambique

Open Access Article; Published online: 7 March 2019Agrobacterium deltaense strain CNPSo 3391 was isolated from a soybean nodule in Mozambique. Its genome size was estimated at 4,926,588 bp. This isolate carries several coding sequences for stress tolerance, but no identifiable nodulation or virulence genes. Possible ecological roles of bacteria isolated from legume nodules and closely related to Agrobacterium are discussed

Effects of feed particle size on energy values for broiler chickens at various ages

The objective of this study was to evaluate the effects of various geometric mean diameters (GMDs) of particles of corn, pelleted soybean meal and a corn-soy mixture in the proportion of 70% and 30%, respectively, on the nutritional value of the feeds. The study evaluated energy consumption, the contents of apparent metabolizable energy (AME) and AME corrected for nitrogen balance (AMEn) and the metabolizability coefficients for broiler chickens at various ages. A total of 540 Cobb 500 male broilers were housed in metabolic cages (experimental units). Trials were performed separately with each feed. A completely randomized design was used with four treatments, namely corn with 573, 636, 851, and 1012 μm GMDs; pelleted soybean meal with 538, 550, 665, and 741 μm GMDs; and the corn-soy mixture with 627, 658, 893, and 1040 μm GMDs. Birds were evaluated on days 1 - 10, 11 - 20, 21 - 30, and 31 - 40. Larger GMDs resulted in lower energy consumption. From 1 to 10 days, birds consumed less metabolizable energy than older birds. Birds fed corn from days 1 to 10 had higher metabolizable energy (P <0.05) with increasing GMD up to 1042 μm. However, the results varied, depending on the feed and its combinations. The use of coarse particles could reduce the costs of grinding, and would have few effects on the metabolizable energy of broiler chickens

Dressing the nucleon in a dispersion approach

We present a model for dressing the nucleon propagator and vertices. In the model the use of a K-matrix approach (unitarity) and dispersion relations (analyticity) are combined. The principal application of the model lies in pion-nucleon scattering where we discuss effects of the dressing on the phase shifts.Comment: 17 pages, using REVTeX, 6 figure

Generalized Polarizabilities of the Nucleon in Chiral Effective Theories

Using the techniques of chiral effective field theories we evaluate the so called generalized polarizabilities of the nucleon, which characterize the structure dependent components in virtual Compton scattering (VCS) as probed in the electron scattering reaction e N \to e' N gamma. Results are given for both spin-dependent and spin-independent structure effects to O(p^3) in SU(2) Heavy Baryon Chiral Perturbation Theory and to O(epsilon^3) in the SU(2) Small Scale Expansion. Finally we compare our calculations with results from the pioneering VCS experiment on the proton from Mainz.Comment: 39 pages, 12 figures, revte

The Intentional Use of Service Recovery Strategies to Influence Consumer Emotion, Cognition and Behaviour

Service recovery strategies have been identified as a critical factor in the success of. service organizations. This study develops a conceptual frame work to investigate how specific service recovery strategies influence the emotional, cognitive and negative behavioural responses of . consumers., as well as how emotion and cognition influence negative behavior. Understanding the impact of specific service recovery strategies will allow service providers' to more deliberately and intentionally engage in strategies that result in positive organizational outcomes. This study was conducted using a 2 x 2 between-subjects quasi-experimental design. The results suggest that service recovery has a significant impact on emotion, cognition and negative behavior. Similarly, satisfaction, negative emotion and positive emotion all influence negative behavior but distributive justice has no effect

Identification of genetic risk variants for deep vein thrombosis by multiplexed next-generation sequencing of 186 hemostatic/pro-inflammatory genes

BACKGROUND: Next-generation DNA sequencing is opening new avenues for genetic association studies in common diseases that, like deep vein thrombosis (DVT), have a strong genetic predisposition still largely unexplained by currently identified risk variants. In order to develop sequencing and analytical pipelines for the application of next-generation sequencing to complex diseases, we conducted a pilot study sequencing the coding area of 186 hemostatic/proinflammatory genes in 10 Italian cases of idiopathic DVT and 12 healthy controls. RESULTS: A molecular-barcoding strategy was used to multiplex DNA target capture and sequencing, while retaining individual sequence information. Genomic libraries with barcode sequence-tags were pooled (in pools of 8 or 16 samples) and enriched for target DNA sequences. Sequencing was performed on ABI SOLiD-4 platforms. We produced > 12 gigabases of raw sequence data to sequence at high coverage (average: 42X) the 700-kilobase target area in 22 individuals. A total of 1876 high-quality genetic variants were identified (1778 single nucleotide substitutions and 98 insertions/deletions). Annotation on databases of genetic variation and human disease mutations revealed several novel, potentially deleterious mutations. We tested 576 common variants in a case-control association analysis, carrying the top-5 associations over to replication in up to 719 DVT cases and 719 controls. We also conducted an analysis of the burden of nonsynonymous variants in coagulation factor and anticoagulant genes. We found an excess of rare missense mutations in anticoagulant genes in DVT cases compared to controls and an association for a missense polymorphism of FGA (rs6050; p = 1.9 7 10(-5), OR 1.45; 95% CI, 1.22-1.72; after replication in > 1400 individuals). CONCLUSIONS: We implemented a barcode-based strategy to efficiently multiplex sequencing of hundreds of candidate genes in several individuals. In the relatively small dataset of our pilot study we were able to identify bona fide associations with DVT. Our study illustrates the potential of next-generation sequencing for the discovery of genetic variation predisposing to complex diseases

Customer emotions in service failure and recovery encounters

Emotions play a significant role in the workplace, and considerable attention has been given to the study of employee emotions. Customers also play a central function in organizations, but much less is known about customer emotions. This chapter reviews the growing literature on customer emotions in employee–customer interfaces with a focus on service failure and recovery encounters, where emotions are heightened. It highlights emerging themes and key findings, addresses the measurement, modeling, and management of customer emotions, and identifies future research streams. Attention is given to emotional contagion, relationships between affective and cognitive processes, customer anger, customer rage, and individual differences

Wholesale pricing in a small open economy

This paper addresses the empirical analysis of wholesale profit margins using data of the Dutch wholesale sector, 1986. At the heart of the analysis is the typical nature of wholesale production: wholesalers do not produce a tangible product, but offer a service capacity. This has an immediate impact on the identification, interprelation and measurement of determinants of profit variations. A model is set up to explain variations in wholesale profit margins, which is inspired by two widely applied approaches to industry pricing: the behavioural mark-up model and the marginalist price-cost model