Analysis of particle size distribution in municipal wastewaters

Innovative membrane filtration plants for municipal wastewaters are being developed and need the support of reliable filtration models in the designing phase. In the past, semi-empirical filtration models for membrane processes have been proposed. At present, the most prominent works point out the importance of particle poly-dispersity in the development of reliable models but fail into the implementation of probability density functions (PDFs) capable of an accurate fitting of the experimental particle size distribution (PSD). We report the experimental PSDs of two different municipal wastewater samples, obtained through the laser diffraction technique. The experimental results show that the laser diffraction technique can characterize wastewater particle dimensions both in the colloidal and supra-colloidal regions. The experimental study is complemented by a comparative analysis in which many PDFs are used to fit the experimental PSDs through a least-squares approach. Some of these PDFs are proposed here for the first time to fit experimental wastewater PSDs. Among the PDFs considered for the statistical modeling, the three-parameter lognormal and the Burr PDFs are demonstrated to provide satisfactory fitting, whereas the other considered functions fail. This result is confirmed by the analysis of both the available wastewater samples

Ocorrência e caracterização de bactérias isoladas de nódulos de amendoinzeiro (Arachis hypogaea L.) em solos paranaenses, Brasil.

RESUMO: O objetivo deste trabalho foi avaliar a ocorrência e caracterizar morfofisiologicamente e geneticamente bactérias isoladas de nódulos de amendoinzeiro (Arachis hypogaea L.), em solos paranaenses, Brasil. Em amostras de 36 municípios representativos do estado Paraná foi observada nodulação do amendoim em 84,4%, independente se em áreas cultivadas, ou em florestas. Nos testes de caracterização morfofisiológica de 44 estirpes autenticadas, 75% das estirpes apresentaram taxa de crescimento rápido em meio de cultura com manitol; 25 acidificaram o meio, cinco alcalinizaram e 14 não resultaram em modificação do pH. A capacidade de produzir sideróforos foi observada em 43% das estirpes e 16% foram capazes de solubilizar fosfato de cálcio em meio de cultura. O sequenciamento do gene 16S rRNA mostrou a formação de três principais grupos, correspondentes a três filos: Alfaproteobacteria, Betaproteobacteria e Firmicutes. O gênero com maior ocorrência foi o Bacillus

Azospirillum brasilense associado a pós de rocha no cultivo de milho orgânico.

A inoculação com Azospirillum brasilense pode auxiliar na nutrição nitrogenada em milho, sobretudo em condições de cultivo orgânico em que fontes solúveis de N são evitadas. Além da fonte biológica de N, a associação dessa bactéria pode melhorar o estado nutricional em relação a outros nutrientes, quando as plantas recebem fontes de baixa solubilidade como pós de rocha. O objetivo foi avaliar o efeito da inoculação de A. brasilense em milho sob sistema de produção orgânico, em combinação com pós de basalto de três origens geológicas e fosfato natural fosforita. O experimento foi realizado em duas localidades, Cruz Machado- PR e Irineópolis-SC, no segundo ano de avaliação. O delineamento experimental foi em blocos casualizados, com seis tratamentos de pós de rocha (parcela), em milho inoculado ou não com A. brasilense (subparcela), com quatro repetições. Os tratamentos foram: 3 Mg/ha de pó de basalto I, II e III, 1 Mg/ha de fosfato natural, 3 Mg/ha de pó de basalto I + 1 Mg/ha de fosfato natural e um controle sem rochagem. O milho (variedade crioula Caiano) foi consorciado com feijão de porco na entrelinha. No florescimento, avaliaram-se os teores de N e P nas folhas e a ocorrência de bactérias diazotróficas endofíticas na região da coroa das plantas. Os dados foram submetidos à ANOVA e aplicado o teste Tukey a 5 %. Na área de Cruz Machado, o teor de N nas plantas foi maior nos tratamentos inoculados com A. brasilense, exceto na testemunha sem pó de rocha. Em Irineópolis, não houve efeito dos tratamentos ou subtratamentos. Já o teor de P nas plantas não foi alterado pelos tratamentos ou subtratamentos em Cruz Machado, mas em Irienópolis o milho inoculado apresentou maior teor de P que o não inoculado, e quando inoculadas, as plantas do tratamento com pó de basalto + fosfato natural apresentaram maior teor de P em relação às da testemunha. No geral, nas duas áreas houve maior densidade de bactérias diazotróficas endofíticas nas plantas inoculadas, o que inclui não só A. brasilense, mas também outras bactérias que podem estar contribuindo para a fixação do N2 e promoção do crescimento vegetal. A inoculação com A. brasilense foi eficiente na melhora da nutrição do milho não apenas em relação ao N, mas em alguns casos também em relação ao P, casos em que também houve maior colonização por bactérias diazotróficas no interior da planta.Resumo, 1400-1

Hypertrophic Adenoid Is A Major Infection Site Of Human Bocavirus 1.

Human bocavirus 1 (HBoV1) is associated with respiratory infections worldwide, mainly in children. Similar to other parvoviruses, it is believed that HBoV1 can persist for long periods of time in humans, probably through maintaining concatemers of the virus single-stranded DNA genome in the nuclei of infected cells. Recently, HBoV-1 was detected in high rates in adenoid and palatine tonsils samples from patients with chronic adenotonsillar diseases, but nothing is known about the virus replication levels in those tissues. A 3-year prospective hospital-based study was conducted to detect and quantify HBoV1 DNA and mRNAs in samples of the adenoids (AD), palatine tonsils (PT), nasopharyngeal secretions (NPS), and peripheral blood (PB) from patients undergoing tonsillectomy for tonsillar hypertrophy or recurrent tonsillitis. HBoV1 was detected in 25.3% of the AD samples, while the rates of detection in the PT, NPS, and PB samples were 7.2%, 10.5%, and 1.7%, respectively. The viral loads were higher in AD samples, and 27.3% of the patients with HBoV had mRNA detectable in this tissue. High viral loads and detectable mRNA in the AD were associated with HBoV1 detection in the other sample sites. The adenoids are an important site of HBoV1 replication and persistence in children with tonsillar hypertrophy. The adenoids contain high HBoV1 loads and are frequently positive for HBoV mRNA, and this is associated with the detection of HBoV1 in secretions.523030-

Dental Health and Mortality in People With End-Stage Kidney Disease Treated With Hemodialysis: A Multinational Cohort Study

Background Dental disease is more extensive in adults with chronic kidney disease, but whether dental health and behaviors are associated with survival in the setting of hemodialysis is unknown. Study Design Prospective multinational cohort. Setting & Participants 4,205 adults treated with long-term hemodialysis, 2010 to 2012 (Oral Diseases in Hemodialysis [ORAL-D] Study). Predictors Dental health as assessed by a standardized dental examination using World Health Organization guidelines and personal oral care, including edentulousness; decayed, missing, and filled teeth index; teeth brushing and flossing; and dental health consultation. Outcomes All-cause and cardiovascular mortality at 12 months after dental assessment. Measurements Multivariable-adjusted Cox proportional hazards regression models fitted with shared frailty to account for clustering of mortality risk within countries. Results During a mean follow-up of 22.1 months, 942 deaths occurred, including 477 cardiovascular deaths. Edentulousness (adjusted HR, 1.29; 95% CI, 1.10-1.51) and decayed, missing, or filled teeth score ≥ 14 (adjusted HR, 1.70; 95% CI, 1.33-2.17) were associated with early all-cause mortality, while dental flossing, using mouthwash, brushing teeth daily, spending at least 2 minutes on oral hygiene daily, changing a toothbrush at least every 3 months, and visiting a dentist within the past 6 months (adjusted HRs of 0.52 [95% CI, 0.32-0.85], 0.79 [95% CI, 0.64-0.97], 0.76 [95% CI, 0.58-0.99], 0.84 [95% CI, 0.71-0.99], 0.79 [95% CI, 0.65-0.95], and 0.79 [95% CI, 0.65-0.96], respectively) were associated with better survival. Results for cardiovascular mortality were similar. Limitations Convenience sample of clinics. Conclusions In adults treated with hemodialysis, poorer dental health was associated with early death, whereas preventive dental health practices were associated with longer survival

Target for improvement: a cluster randomised trial of public involvement in quality-indicator prioritisation (intervention development and study protocol)

<p>Abstract</p> <p>Background</p> <p>Public priorities for improvement often differ from those of clinicians and managers. Public involvement has been proposed as a way to bridge the gap between professional and public clinical care priorities but has not been studied in the context of quality-indicator choice. Our objective is to assess the feasibility and impact of public involvement on quality-indicator choice and agreement with public priorities.</p> <p>Methods</p> <p>We will conduct a cluster randomised controlled trial comparing quality-indicator prioritisation with and without public involvement. In preparation for the trial, we developed a 'menu' of quality indicators, based on a systematic review of existing validated indicator sets. Participants (public representatives, clinicians, and managers) will be recruited from six participating sites. In intervention sites, public representatives will be involved through direct participation (public representatives, clinicians, and managers will deliberate together to agree on quality-indicator choice and use) and consultation (individual public recommendations for improvement will be collected and presented to decision makers). In control sites, only clinicians and managers will take part in the prioritisation process. Data on quality-indicator choice and intended use will be collected. Our primary outcome will compare quality-indicator choice and agreement with public priorities between intervention and control groups. A process evaluation based on direct observation, videorecording, and participants' assessment will be conducted to help explain the study's results. The marginal cost of public involvement will also be assessed.</p> <p>Discussion</p> <p>We identified 801 quality indicators that met our inclusion criteria. An expert panel agreed on a final set of 37 items containing validated quality indicators relevant for chronic disease prevention and management in primary care. We pilot tested our public-involvement intervention with 27 participants (11 public representatives and 16 clinicians and managers) and our study instruments with an additional 21 participants, which demonstrated the feasibility of the intervention and generated important insights and adaptations to engage public representatives more effectively. To our knowledge, this study is the first trial of public involvement in quality-indicator prioritisation, and its results could foster more effective upstream engagement of patients and the public in clinical practice improvement.</p> <p>Trial registration</p> <p><a href="http://www.trialregister.nl/trialreg/admin/rctview.asp?TC=2496">NTR2496</a> (Netherlands National Trial Register, <url>http://www.trialregister.nl</url>).</p

Soluble iron nutrients in Saharan dust over the central Amazon rainforest

The intercontinental transport of aerosols from the Sahara desert plays a significant role in nutrient cycles in the Amazon rainforest, since it carries many types of minerals to these otherwise low-fertility lands. Iron is one of the micronutrients essential for plant growth, and its long-range transport might be an important source for the iron-limited Amazon rainforest. This study assesses the bioavailability of iron Fe(II) and Fe(III) in the particulate matter over the Amazon forest, which was transported from the Sahara desert (for the sake of our discussion, this term also includes the Sahel region). The sampling campaign was carried out above and below the forest canopy at the ATTO site (Amazon Tall Tower Observatory), a near-pristine area in the central Amazon Basin, from March to April 2015. Measurements reached peak concentrations for soluble Fe(III) (48 ng m&minus;3), Fe(II) (16 ng m&minus;3), Na (470 ng m&minus;3), Ca (194 ng m&minus;3), K (65 ng m&minus;3), and Mg (89 ng m&minus;3) during a time period of dust transport from the Sahara, as confirmed by ground-based and satellite remote sensing data and air mass backward trajectories. Dust sampled above the Amazon canopy included primary biological aerosols and other coarse particles up to 12 &micro;m in diameter. Atmospheric transport of weathered Saharan dust, followed by surface deposition, resulted in substantial iron bioavailability across the rainforest canopy. The seasonal deposition of dust, rich in soluble iron, and other minerals is likely to assist both bacteria and fungi within the topsoil and on canopy surfaces, and especially benefit highly bioabsorbent species. In this scenario, Saharan dust can provide essential macronutrients and micronutrients to plant roots, and also directly to plant leaves. The influence of this input on the ecology of the forest canopy and topsoil is discussed, and we argue that this influence would likely be different from that of nutrients from the weathered Amazon bedrock, which otherwise provides the main source of soluble mineral nutrients