トウイン ニオケル ヒカリ センタクテキ ゼンリツセン ジョウサンジュツ PVP ノ リンショウテキ ケントウ

Recently, photoselective vaporization of the prostate （PVP） has become an increasingly popular option for the treatment of lower urinary tract symptoms （LUTS） secondary to benign prostatic hyperplasia （BPH）, due to the associated lower morbidity. In the present study, we present the results of our experience with １２０-W high-performance system （HPS） laser PVP. From July ２０１２ to March ２０１５, a total of ６０ consecutive patients, with a mean age of ７０．０ years, underwent PVP. The mean prostate volume, the mean operative duration, and the mean hemoglobin decrease was ５８．８ cm３, ９３．５ minutes and ０．４ g/dl, respectively. Significant improvements were observed postoperatively in the International Prostate Symptom Score, quality of life score, maximal urinary flow rate, and postvoid residual urine volume. All of ７ patients with chronic urinary retention have become catheter-free. No major complication occurred intraoperatively or postoperatively. Although ７ patients （１１．７％） required recatheterization postoperatively, the catheters were able to be removed from all of them in a few days. The mean postoperative catheterization time including recatheterization was ３４．１ hours. PVP with a １２０-W HPS laser is considered to be effective and safe for treatment of LUTS secondary to BPH

Overexpression of wild-type Akt1 promoted insulin-stimulated p70S6 kinase (p70S6K) activity and affected GSK3 beta regulation, but did not promote insulin-stimulated GLUT4 translocation or glucose transport in L6 myotubes

We have developed a simple, direct and sensitive method to detect GLUT4 on the cell surface. Using this system, we found that PI3-kinase plays a key role in the signaling pathway of insulin-stimulated GLUT4 translocation. One of the down stream effectors of PI3-kinase is serine-threonine kinase Akt (protein kinase B, RAK-PK), but the involvement of Akt in insulin-stimulated GLUT4 translocation is controversial. To investigate whether Akt1 regulates insulin-stimulated GLUT4 translocation and glucose uptake in L6 myotubes, we established L6 myotubes stably expressing c-myc epitope-tagged GLUT4 (GLUT4myc) and mouse wild type (WT) Akt1. We found that overexpression of WT Akt1 promoted insulin-stimulated p70S6 kinase (p70S6K) activity and increased the basal activity of GSK3β, but did not promote insulin-stimulated GLUT4translocation or glucose uptake. These data supported the result that Akt is not a main signaling molecule to transmit the signal of insulin-stimulated GLUT4 translocation or glucose uptake from insulin-activated PI3-kinase

Enrichment of Education and Research at Hiroshima University through the Top Global University Project

Hiroshima University named its Super (Top) Global University Project the “Hiroshima University Global Campus Expansion and Innovation Initiative”. Under this title, the university has been endeavoured to strengthen both its educational capability and research function ability with the goal of becoming by 2023 one of the world’s top 100 universities. One of the most important missions of a top university is to produce internationally ducated/experienced, globally capable human resources who have flexibility to face and resolve unpredictable issues. This book exhibits some of highlighted activities undertaken by Hiroshima University to achieve the above mentioned goals.はしがき 西谷 元 …i 序章 はじめに －大学グローバル化の諸相－ 丸山 文裕 …1 第1章 広島大学の目標達成型重要業績指標AKPI 相田 美砂子…7 第2章 国際的な教育質保証に向けた取り組み 　－Student Experience in the Research University (SERU)－ 渡邉 聡 …25 第3章 留学効果の客観的測定・プログラムの質保証 　－The Beliefs, Events, and Values Inventory (BEVI-j )－ 西谷 元 …45 第4章 三階層ティーチングアシスタント制度「Hirodai TA」の理念と実践 　－多様性を保証するグローバル・バリアフリー・キャンパス構想の実現を目指して－ 丸山 恭司・戴 容秦思・中野 登志美 …71 第5章 英語個人別期待値の設定 　－語学力向上の動機づけまたSGU 目標達成の一助として－ 西谷 元・渡邉 恵…9

STING signalling is terminated through ESCRT-dependent microautophagy of vesicles originating from recycling endosomes

STING炎症シグナルの終結分子機構 --新規細胞内分解システムの発見--. 京都大学プレスリリース. 2023-03-14.Stimulator of interferon genes (STING) is essential for the type I interferon response against a variety of DNA pathogens. Upon emergence of cytosolic DNA, STING translocates from the endoplasmic reticulum to the Golgi where STING activates the downstream kinase TBK1, then to lysosome through recycling endosomes (REs) for its degradation. Although the molecular machinery of STING activation is extensively studied and defined, the one underlying STING degradation and inactivation has not yet been fully elucidated. Here we show that STING is degraded by the endosomal sorting complexes required for transport (ESCRT)-driven microautophagy. Airyscan super-resolution microscopy and correlative light/electron microscopy suggest that STING-positive vesicles of an RE origin are directly encapsulated into Lamp1-positive compartments. Screening of mammalian Vps genes, the yeast homologues of which regulate Golgi-to-vacuole transport, shows that ESCRT proteins are essential for the STING encapsulation into Lamp1-positive compartments. Knockdown of Tsg101 and Vps4, components of ESCRT, results in the accumulation of STING vesicles in the cytosol, leading to the sustained type I interferon response. Knockdown of Tsg101 in human primary T cells leads to an increase the expression of interferon-stimulated genes. STING undergoes K63-linked ubiquitination at lysine 288 during its transit through the Golgi/REs, and this ubiquitination is required for STING degradation. Our results reveal a molecular mechanism that prevents hyperactivation of innate immune signalling, which operates at REs

Cytokinin Activities of N-(Purin-6-yl) amino Acids, N-(Purin-6-yl) peptides and Related Compounds (A. NATURAL SCIENCE)

Four N-(purin-6-yl) amino acids, six N-(purin-6-yl) peptides, three ethyl esters of the latter, and some related compounds were tested for their cytokinin activities by the tobacco callus bioassay and lettuce seed germination. Among the N-(purin-6-yl) amino acids, N-(purin-6-yl)-L-phenylalanine exhibited a weak cytokinin activity at 100μM in the tobacco callus bioassay, although around 10000 times less active than kinetin, but no any activity in lettuce seed germination. The N-(purin-6-yl) peptides tested were all inactive in both bioassays. However, esterification with the ethyl group in N-(purin-6-yl) glycylglycine and N-(purin-6-yl)-L-phenylalanylglycine enhanced the cytokinin activity; they were weakly active at 100μM in the tobacco callus bioassay, but inactive in lettuce seed germination. (±)-2-Methyl-4-(purin-6-ylamino) butyric acid was active at 1-100μM in the tobacco callus bioassay and its methyl ester showed a stronger cytokinin activity. Analogous amino acid or peptide derivatives with a naphthalene or pyrimidine ring instead of the purine ring were inactive

Enrichment of Education and Research at Hiroshima University through the Top Global University Initiative: EBPM and Quality Assurance

Hiroshima University named its Super (Top) Global University Initiative the “Hiroshima University Global Campus Expansion and Innovation Initiative”. The Initiative is implemented as a university-wide project in which all faculties and departments are implicated in the pursuit of its goals. In this process, Hiroshima University collected extensive information on the educational, research and social contributions of the university, and made policy decisions on the basis of this objective data. In addition, Hiroshima University developed a system to monitor the achievements of the top 100 universities by developing its own KPIs, and a system that optimally allocates education and research resources. In this book, we review various aspects of Hiroshima University’s Super (Top) Global University Initiative. Part 1 focuses on its unique approaches related to IR and EBPM. In Part 2, we examine various education programmes and consider the implementation of the Initiative.はしがき… 西谷 元 i 第一部 教育・研究基盤の分析・目標設定・改革 第1章 広島大学の目標達成型重要業績指標AKPI®と教員エフォート指標BKPI®… 相田 美砂子 1 第2章 国際的な教育質保証に向けた取り組み（続編）… 渡邉 聡・村澤 昌崇・安部 保海・梅下 健一郎・中尾 走 11 第3章 BEVI を用いた留学効果の客観的測定 －客観的データに基づく留学プログラムの質保証－… 西谷 元 39 第4章 EBPM に基づく学生の英語運用能力の向上 －エビデンスに基づく教育プログラムの改革・実施・成果－… 西谷 元 53 第二部 国際標準の教育の提供 第5章 広島大学森戸国際高等教育学院とその新たな取組み… 荒見 泰史 67 第6章 三階層ティーチング・アシスタント制度「Hirodai TA」の展開 －多様性を保証するグローバル・バリアフリー・キャンパス構想に向けた成果と課題－… 丸山 恭司・佐藤 万知・河本 尚枝・島津 礼子・小澤 郁美・Simona Zollet 87 第7章 グローバル・キャンパスで学ぶ ―総合科学部国際共創学科の取組みと課題… 柴田 美紀・フンク カロリン 105 第8章 Global Peace Leadership Program… 小澤 孝一郎 12

Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome.

The evolution of land flora transformed the terrestrial environment. Land plants evolved from an ancestral charophycean alga from which they inherited developmental, biochemical, and cell biological attributes. Additional biochemical and physiological adaptations to land, and a life cycle with an alternation between multicellular haploid and diploid generations that facilitated efficient dispersal of desiccation tolerant spores, evolved in the ancestral land plant. We analyzed the genome of the liverwort Marchantia polymorpha, a member of a basal land plant lineage. Relative to charophycean algae, land plant genomes are characterized by genes encoding novel biochemical pathways, new phytohormone signaling pathways (notably auxin), expanded repertoires of signaling pathways, and increased diversity in some transcription factor families. Compared with other sequenced land plants, M. polymorpha exhibits low genetic redundancy in most regulatory pathways, with this portion of its genome resembling that predicted for the ancestral land plant. PAPERCLIP