Exploring adaptive capacity: Observations from the vulnerable human-coastal environmental system of the Bay of Bengal in India

This article presents the factors that help build the adaptive capacity of individuals to reduce vulnerability from natural threats. The findings are based on primary data on individuals engaged in various livelihood practices in the Digha-Sankarpur- Mandarmoni region along the eastern coastline of the Bay of Bengal in India. Coastal communities have their individual perceptions about their vulnerability to natural threats and associated risks to various assets. Based on perception survey responses, “adaptive capacity” is measured and the determining factors are analyzed with an aim to provide policy guidelines for strengthening the adaptive capacity of people dependent on coastal ecosystem-based livelihoods. Findings show that income security for individuals in coastal systems can be achieved through a livelihood diversification strategy. The scope for individuals to build personal savings, have access to financial institutions, and engage in collaborative actions with immediate neighbors in the community help to prevent multiple losses at the individual level aids in their adaptive capacity. Government actions to provide institutional and governance support and incentives to promote livelihood diversification and personal savings would enhance adaptive capacity through institutional actions. Public investment in public goods and services, including protection and monitoring of natural coastal ecosystems, infrastructure development, technological intervention, providing access to information, and community empowerment, have important roles to play in enhancing adaptive capacity

Rice oxalate oxidasegene driven by green tissue-specific promoter increases tolerance to sheath blight pathogen (Rhizoctonia solani) in transgenic rice

Rice sheath blight, caused by the necrotrophic fungus Rhizoctonia solani, is one of the most devastating and intractable diseases of rice, leading to a significant reduction in rice productivity worldwide. In this article, in order to examine sheath blight resistance, we report the generation of transgenic rice lines overexpressing the rice oxalate oxidase 4 (Osoxo4) gene in a green tissue-specific manner which breaks down oxalic acid (OA), the pathogenesis factor secreted by R. solani. Transgenic plants showed higher enzyme activity of oxalate oxidase (OxO) than nontransgenic control plants, which was visualized by histochemical assays and sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Transgenic rice leaves were more tolerant than control rice leaves to exogenous OA. Transgenic plants showed a higher level of expression of other defence-related genes in response to pathogen infection. More importantly, transgenic plants exhibited significantly enhanced durable resistance to R. solani. The overexpression of Osoxo4 in rice did not show any detrimental phenotypic or agronomic effect. Our findings indicate that rice OxO can be utilized effectively in plant genetic manipulation for sheath blight resistance, and possibly for resistance to other diseases caused by necrotrophic fungi, especially those that secrete OA. This is the first report of the expression of defence genes in rice in a green tissue-specific manner for sheath blight resistance

Development of Novel Bis(indolyl)-hydrazide–Hydrazone Derivatives as Potent Microtubule-Targeting Cytotoxic Agents against A549 Lung Cancer Cells

The biological significance of microtubules makes them a validated target of cancer therapy. In this study, we have utilized indole, an important pharmacological scaffold, to synthesize novel bis­(indolyl)-hydrazide–hydrazone derivatives (NMK-BH compounds) and recognized NMK-BH3 as the most effective one in inhibiting A549 cell proliferation and assembly of tissue-purified tubulin. Cell viability experiments showed that NMK-BH3 inhibited proliferation of human lung adenocarcinoma (A549) cells, normal human lung fibroblasts (WI38) and peripheral blood mononuclear cells (PBMC) with IC₅₀ values of ∼2, 48.5, and 62 μM, respectively. Thus, the relatively high cytotoxicity of NMK-BH3 toward lung carcinoma (A549) cells over normal lung fibroblasts (WI38) and PBMC confers a therapeutic advantage of reduced host toxicity. Flow cytometry, Western blot, and immunofluorescence studies in the A549 cell line revealed that NMK-BH3 induced G2/M arrest, mitochondrial depolarization, and apoptosis by depolymerizing the cellular interphase and spindle microtubules. Consistent with these observations, study in <i>cell free system</i> revealed that NMK-BH3 inhibited the microtubule assembly with an IC₅₀ value of ∼7.5 μM. The tubulin–ligand interaction study using fluorescence spectroscopy indicated that NMK-BH3 exhibited strong and specific tubulin binding with a dissociation constant of ∼1.4 μM at a single site, very close to colchicine site, on β-tubulin. Collectively, these findings explore the cytotoxic potential of NMK-BH3 by targeting the microtubules and inspire its development as a potential candidate for lung cancer chemotherapy