Growth of Listeria Monocytogenes in ready to eat salads at different storage temperatures and valuation of virulence genes expression

BACKGROUND: Vegetables are major components of a healthy and balanced diet. However, 25% of foodborne diseases are linked to the consumption of vegetables. STUDY DESIGN: The aim of this work was to assess the microbiological risks associated with consumption of ready to eat salads (RTE). METHODS: Microbiological challenge tests were carried out for the evaluation of the L. monocytogenes growth potential in RTE salads stored at different temperatures. RESULTS: The results indicate that L.monocytogenes was able to grow (δ ≥ 0.5) in all storage conditions considered at the end of shelf life. In order to evaluate the virulence role of L. monocytogenes, the temperature-dependent transcription of major virulence genes was also investigated by RT-PCR. CONCLUSIONS: The microbiological challenge test allowed us to confirm, as also demonstrated by other authors, that RTE salads are able to support the growth of L. monocytogenes strains (d δ≥ 0.5) stored under different temperatures

Analytical representation of architectural built heritage. A Sketch-to-Bim approach

HBIM methodology is increasingly used for the management of all aspects of architectural heritage, from survey and analysis to conservation, management and restoration issues. Application of HBIM are the so-called Scan-to-BIM processes in which the artifact is surveyed with digital techniques of laser scanning and photogrammetry. These techniques result in point clouds, the basis of the subsequent process of informative and geometric modelling of the artifact. The resulting "smart models" are composed of parametric objects rich in information that can be easily updated at any time. The proposed methodology aims at integrating a study of architectural orders, whose results become preparatory to the subsequent phases of survey and modeling, to the classic Scan-to-BIM workflows. In particular, in the modeling these results have allowed a more targeted choice of techniques used. The method has been applied to the atrium of the former Jesuit College of Santa Croce in Cagliari, which today hosts one of the seats of the Faculty of Engineering and Architecture of the University of Cagliari; in particular, the atrium of the east body of the former Jesuit College, designed by the Piedmontese architect Antonio Felice De Vincenti, has been modelled

The newer opioid agonist treatment with lower substitutive opiate doses is associated with better toxicology outcome than the older harm reduction treatment

Background: Charge-free heroin use disorder treatment in Italy follows two main approaches, i.e., harm reduction treatment (HRT) strategy in community low-threshold facilities for drug addiction and opioid agonist treatment (OAT) in high-threshold facilities for opioid addiction, focusing on pharmacological maintenance according to the Dole and Nyswander strategy. We aimed to compare the impact of HRT and OAT on patient outcome, as assessed through negativity for drugs on about 1-year urinalyses. Methods: We examined retrospectively the urinalyses of HRT and OAT patients for which at least four randomly sampled urinalyses per month were available for about 1 year, during which patients were undergoing methadone or buprenorphine maintenance; urinalyses focused on heroin, cocaine, cannabinoids, and their metabolites. Results: Included were 189 HRT and 58 OAT patients. The latter were observed for a significantly longer period. There was a higher proportion of heroin- and cocaine-clean urinalyses in OAT patients, with cocaine-clean urinalyses discriminating best between the two groups. OAT patients were older, with longer dependence duration, more severe addiction history, and received lower methadone doses. Buprenorphine maintenance was more often associated with heroin-clean urinalyses. The higher the methadone doses, the lower were the percentage of heroin-clean urinalyses in HRT patients (negative correlation). Conclusions: The OAT approach was related to higher recovery and polyabuse abstinence rates compared to the HRT approach, despite greater severity of substance use, psychiatric and physical comorbidities. Our results are consistent with the possibility to use lower maintenance opiate doses (after induction and stabilization in methadone treatment according to Dole and Nyswander methodology) in treating heroin addiction. This seemed to be impossible adopting the currently accepted HRT model

Effect of external pressure on the magnetic properties of RRCoAsO (RR = La, Pr, Sm): a μ\muSR study

We report on a detailed investigation of the itinerant ferromagnets LaCoAsO, PrCoAsO and SmCoAsO performed by means of muon spin spectroscopy upon the application of external hydrostatic pressures $p$ up to $2.4$ GPa. These materials are shown to be magnetically hard in view of the weak dependence of both critical temperatures $T_{C}$ and internal fields at the muon site on $p$. In the cases $R$ = La and Sm, the behaviour of the internal field is substantially unaltered up to $p = 2.4$ GPa. A much richer phenomenology is detected in PrCoAsO instead, possibly associated with a strong $p$ dependence of the statistical population of the two different crystallographic sites for the muon. Surprisingly, results are notably different from what is observed in the case of the isostructural compounds $R$CoPO, where the full As/P substitution is already inducing a strong chemical pressure within the lattice but $p$ is still very effective in further affecting the magnetic properties.Comment: 8 pages, 9 figure

Singling out the effect of quenched disorder in the phase diagram of cuprates

We investigate the specific influence of structural disorder on the suppression of antiferromagnetic order and on the emergence of cuprate superconductivity. We single out pure disorder, by focusing on a series of Y$_{z}$Eu$_{1-z}$Ba$_2$Cu$_3$O$_{6+y}$ samples at fixed oxygen content $y=0.35$, in the range $0\le z\le 1$. The gradual Y/Eu isovalent substitution smoothly drives the system through the Mott-insulator to superconductor transition from a full antiferromagnet with N\'eel transition $T_N=320$ K at $z=0$ to a bulk superconductor with superconducting critical temperature $T_c=18$ K at $z=1$, YBa$_2$Cu$_3$O$_{6.35}$. The electronic properties are finely tuned by gradual lattice deformations induced by the different cationic radii of the two lanthanides, inducing a continuous change of the basal Cu(1)-O chain length, as well as a controlled amount of disorder in the active Cu(2)O$_2$ bilayers. We check that internal charge transfer from the basal to the active plane is entirely responsible for the doping of the latter and we show that superconductivity emerges with orthorhombicity. By comparing transition temperatures with those of the isoelectronic clean system we deterime the influence of pure structural disorder connected with the Y/Eu alloy.Comment: 10 pages 11 figures, submitted to Journal of Physics: Condensed Matter, Special Issue in memory of Prof. Sandro Massid

Comparativo de personajes de la historia de la enfermería brasileña

Este artículo es una reflexión sobre la historia de los profesionales que se destacaron en la enfermería. Para ello, la búsqueda se llevó a cabo en algunos personajes que hicieron mucho para la enfermería y la salud en el país. El análisis de las historias de estas enfermeras puesto de manifiesto en hechos común como: la ruptura de paradigmas relacionado con el matrimonio, la formación en instituciones de renombre, la ocupación en puestos de gran importancia, la participación en asociaciones profesionales expresiva, a si como una relación con la formación de calidad. A través de sus biografías, se puede ver que a pesar del contexto político de la época han favorecido la formación de esas enfermeras, mucho esfuerzo y dedicación fueron necesarios para esas trayectorias de éxito, que cooperaron en la consolidación de la enfermería brasileña.This article presents a reflection about the story of outstanding nursing professionals. For this purpose, a survey was conducted among some people who contributed a lot to nursing and health in Brazil. The analysis of these nurses' stories unveiled facts in common: breaking of paradigms relating to marriage, education at renowned institutions, holding of very important jobs, significant membership in professional associations, and quality education. The bios of these nurses reveal that, while the political scenario then existing contributed to their education, unswerving dedication and diligence, were the key for their successful careers and helped consolidate nursing in Brazil.Este artigo trata de uma reflexão sobre a história de profissionais que se destacaram na Enfermagem. Para isso, foi realizada uma pesquisa sobre algumas personagens que muito realizaram pela Enfermagem e pela saúde no país. A análise das histórias dessas enfermeiras revelou fatos em comum, como: o rompimento de paradigmas relacionados ao casamento; a formação em instituições renomadas; a ocupação em cargos de grande importância; a participação expressiva em entidades de classe, assim como uma relação com a formação de qualidade. Através de suas biografias, percebe-se que, apesar de o contexto político da época ter favorecido na formação dessas enfermeiras, muito esforço e dedicação foram necessários para essas trajetórias de sucesso, que colaboraram para a consolidação da Enfermagem brasileira.UNIFESPUNIFESPSciEL

Protostellar Outflows at the EarliesT Stages (POETS). I. Radio thermal jets at high resolution nearby H2_2O maser sources

Abridged. Methods. We made use of the Karl G. Jansky Very Large Array (VLA) in the B configuration at K band, and in the A configuration at both Ku and C bands, in order to image the radio continuum emission towards 25 H$_2$O maser sites with an angular resolution and thermal rms of the order of $0.1''$ and 10 $\mu$Jy beam$^{-1}$, respectively. These targets add to our pilot study of 11 maser sites presented in Moscadelli et al. (2016). The sample of H$_2$O maser sites was selected among those regions having an accurate distance measurement, obtained through maser trigonometric parallaxes, and H$_2$O maser luminosities in excess of 10$^{-6}$ L$_{\odot}$. Results. We present high-resolution radio continuum images of 33 sources belonging to 25 star-forming regions. In each region, we detect radio continuum emission within a few 1000 au of the H$_2$O masers' position; 50% of the radio continuum sources are associated with bolometric luminosities exceeding 5 $\times$ 10$^{3}$ L$_{\odot}$, including W33A and G240.32$+$0.07. We provide a detailed spectral index analysis for each radio continuum source, based on the integrated fluxes at each frequency, and produce spectral index maps with the multi-frequency-synthesis deconvolution algorithm of CASA. The radio continuum emission traces thermal bremsstrahlung in (proto)stellar winds and jets, with flux densities at 22 GHz below 3 mJy, and spectral index values between $-0.1$ and 1.3. We prove a strong correlation ($r>$0.8) between the radio continuum luminosity (L$_{\rm rad}$) and the H$_2$O maser luminosity (L$_{\rm H_2O}$) of ($\rm L_{8GHz}/mJy\times kpc^2)=10^{3.8}\times(L_{H_2O}/L_{\odot})^{0.74}$. Since H$_2$O masers are excited through shocks driven by (proto)stellar winds and jets, these results provide support to the idea that the radio continuum emission around young stars is dominated by shock-ionization.Comment: 25 pages, 7 figures, 4 tables, accepted by Astronomy & Astrophysic

Releasing cation diffusion in self-limited nanocrystalline defective ceria thin films

Acceptor-doped nanocrystalline cerium oxide thin films are mechanically constrained nano-domains, with film/substrate interfacial strain and chemical doping deadlock mass diffusion.</p

Analysis of Parametric Oscillatory Instability in Power Recycled LIGO Interferometer

We present the analysis of a nonlinear effect of parametric oscillatory instability in power recycled LIGO interferometer with the Fabry-Perot (FP) cavities in the arms. The basis for this effect is the excitation of the additional (Stokes) optical mode and the mirror elastic mode, when the optical energy stored in the main FP cavity main mode exceeds the certain threshold and the frequencies are related so that sum of frequencies of Stokes and elastic modes are approximately equal to frequencyof main mode. The presence of anti-Stokes modes (with frequency approximately equal to sum of frequencies of main and elastic modes) can depress parametric instability. However, it is very likely that the anti-Stokes modes will not compensate the parametric instability completely.Comment: 9 pages, 2 figures. submitted to Physics Letters