586 research outputs found
Estudio cinético de la formación fotoquímica de mono- y trifluoruro de cloro en el sistema fluor-cloro-monofluoruro de cloro
Debido fundamentalmente a la alta reactividad del flúor y de los fluoruros de cloro, la cinética de las reacciones que ocurren en el sistema F2-Cl2-ClF-ClF3 es muy poco conocida, contrariamente a lo que podría suponerse teniendo en cuenta la simplicidad de las especies mencionadas. El sistema puede ser estudiado, no obstante, con relativa facilidad, trabajando con materiales inertes y eliminando toda traza de humedad y de materia orgánica, obteniéndose en general una reproductibilidad satisfactoria. En este trabajo se realiza un estudio sistemático de distintas reacciones entre dichas especies. En particular se estudian, en fase gaseosa, a temperaturas cercanas a la ambiente y por vía fotoquímica, los siguientes sistemas: a) la formación de ClF3 a partir de ClF y F2: F2 + ClF + hV → ClF3; b) la transformación de ClF en ClF3 y Cl2: 3ClF + hv → ClF3 + Cl2; c) la reacción del punto (b) en presencia de Cl2, en condiciones que prácticamente toda la luz incidente es absorbida por Cl2; y d) la formación de ClF y ClF3 a partir de F2 y Cl2 en presencia de ClF: Cl2 + F2 + hv → 2 ClF (con formación de ClF3 por (a) o (c)). El desarrollo de las experiencias realizadas comprende el estudio del efecto de la presión de reactivos, productos e inertes, así como los de la temperatura y la intensidad luminosa sobre la velocidad de reacción. El agregado de oxígeno en algunos ensayos permite asimismo obtener información acerca de la velocidad de consumo de los radicales libres producidos en el sistema durante la irradiación.Fil:San Roman, Enrique A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Wnt Secretion from Epithelial Cells and Subepithelial Myofibroblasts Is Not Required in the Mouse Intestinal Stem Cell Niche In Vivo
Summary Wnt signaling is a crucial aspect of the intestinal stem cell niche required for crypt cell proliferation and differentiation. Paneth cells or subepithelial myofibroblasts are leading candidate sources of the required Wnt ligands, but this has not been tested in vivo. To abolish Wnt-ligand secretion, we used Porcupine (Porcn) conditional-null mice crossed to strains expressing inducible Cre recombinase in the epithelium, including Paneth cells (Villin-CreERT2); in smooth muscle, including subepithelial myofibroblasts (Myh11-CreERT2); and simultaneously in both compartments. Elimination of Wnt secretion from any of these compartments did not disrupt tissue morphology, cell proliferation, differentiation, or Wnt pathway activity. Thus, Wnt-ligand secretion from these cell populations is dispensable for intestinal homeostasis, revealing that a minor cell type or significant and unexpected redundancy is responsible for physiologic Wnt signaling in vivo
Newly Identified Star Clusters in M33. III. Structural Parameters
We present the morphological properties of 161 star clusters in M33 using the
Advanced Camera For Surveys Wide Field Channel onboard the Hubble Space
Telescope using observations with the F606W and F814W filters. We obtain, for
the first time, ellipticities, position angles, and surface brightness profiles
for a significant number of clusters. On average, M33 clusters are more
flattened than those of the Milky Way and M31, and more similar to clusters in
the Small Magellanic Cloud. The ellipticities do not show any correlation with
age or mass, suggesting that rotation is not the main cause of elongation in
the M33 clusters. The position angles of the clusters show a bimodality with a
strong peak perpendicular to the position angle of the galaxy major axis. These
results support the notion that tidal forces are the reason for the cluster
flattening. We fit King and EFF models to the surface brightness profiles and
derive structural parameters including core radii, concentration, half-light
radii and central surface brightness for both filters. The surface brightness
profiles of a significant number of clusters show irregularities such as bumps
and dips. Young clusters (Log age < 8) are notably better fitted by models with
no radial truncation (EFF models), while older clusters show no significant
differences between King or EFF fits. M33 star clusters seem to have smaller
sizes, smaller concentrations, and smaller central surface brightness as
compared to clusters in the MW, M31, LMC and SMC. Analysis of the structural
parameters presents a age-radius relation also detected in other star cluster
systems. The overall analysis shows differences in the structural evolution
between the M33 cluster system and cluster systems in nearby galaxies. These
differences could have been caused by the strong differences in these various
environments.Comment: 30 pages, 12 figures, accepted for publication in MNRA
Engineering cartilage like tissue using polymeric systems derived from 2-ethyl-2-pyrrolidone-methacrylate combined with hyaluronic acid
Hydrogels are potential candidates for Tissue engineering (TE), because of their water content, transport properties, and tissue like physical and chemical behaviour. This project was based on the production and characterization of implantable stimuli responsive scaffolds made of hyaluronic acid (HA) that presents a high water retention character, in combination with 2-ethyl (2-pyrrolidone) methacrylate (EPM), possessing temperature-dependent solubility in water, by bulk polymerization. [...]info:eu-repo/semantics/publishedVersio
Synthesis and characterization of sensitive hydrogels based on semi- interpenetrated networks of poly 2-ethyl-(2-pyrrolidone) methacrylate and hyaluronic acid
Sensitive hydrogels attract interest due to their soft wet appearance and shape response to environmental variations. The synthesis and characterization of semi-interpenetrated hydrogels obtained by radical-induced polymerization of 2-ethyl-(2-pyrrolidone)methacrylate (EPM) in the presence of different concentrations of hyaluronic acid (HA) using N,N′-methylene-bisacrylamide or triethylene glycol dimethacrylate as crosslinker, followed by freeze-drying, are described. Polymeric systems were characterized by NMR, FTIR, SEM, TGA, and DMA. PEPMHA hydrogels' mechanical properties and swelling were found to be intimately related to HA concentration and crosslinker. The swelling response was assessed for temperature and pH variation in order to study the behavior of the hydrogels. We found that the presence of HA in PEPM polymeric systems induced a sensitivity to pH variation rather than temperature. Finally, the biocompatibility profile of the hydrogels was evaluated, using mesenchymal stem cells. Cell adhesion and proliferation results revealed the non-cytotoxicity of the systems. We estimate that PEPMHA hydrogels can be used for applications in tissue engineering and for the controlled release of bioactive compounds.Contract grant sponsor: Marie Curie Early Stage Training Alea Jacta Est; contract grant number: MEST-CT-2004-8104The authors thank David Gomez, Sofia Caridade, and Justyna Chojnacka for their technical support and BIOIBERICA for the supply of hyaluronic acid. This work was carried out under the scope of the European NoE EXPERTISSUES, projects MAT 2007-63355, PTDC/QUI/68804/2006 (FCT), CIBER-BBN and Plan-E 2009-0144. In the memory of Prof. Roberto Sastre
Biomimetic Ca-P coatings Incorporating bisphosphonates produced on starch-based degradable biomaterials
In this study, sodium clodronate, a well-known therapeutic agent from the family
of bisphosphonates (BPs), is incorporated in a biomimetic calcium phosphate (CaP) coating,
previously formed on the surface of a starch-based biomaterial by a sodium silicate
methodology, as a strategy to develop a site-specific drug delivery system for bone tissue
regeneration applications. The effects on the resulting CaP coatings were evaluated in terms of
morphology, chemistry, and structure. The dissolution of Ca and P from the coating and the
release profiles of sodium clodronate was also assessed. As a preliminary approach, this first
study also aimed at evaluating the effects of this BP on the viability of a human osteoblastic
cell line since there is still little information available on the interaction between BPs and this
type of cells. Sodium clodronate was successfully incorporated, at different doses, in the
structure of a biomimetic CaP layer previously formed by a sodium silicate process. This
type of BPs had a stimulatory effect on osteoblastic activity, particularly at the specific
concentration of 0.32 mg/mL. It is foreseen that these coatings can, for instances, be
produced on the surface of degradable polymers and then used for regulating the
equilibrium on osteoblastic/osteoclastic activity, leading to a controlled regenerative effect
at the interface between the biomaterial and bone
Testing the chemical tagging technique with open clusters
Context. Stars are born together from giant molecular clouds and, if we
assume that the priors were chemically homogeneous and well-mixed, we expect
them to share the same chemical composition. Most of the stellar aggregates are
disrupted while orbiting the Galaxy and most of the dynamic information is
lost, thus the only possibility of reconstructing the stellar formation history
is to analyze the chemical abundances that we observe today.
Aims. The chemical tagging technique aims to recover disrupted stellar
clusters based merely on their chemical composition. We evaluate the viability
of this technique to recover co-natal stars that are no longer gravitationally
bound.
Methods. Open clusters are co-natal aggregates that have managed to survive
together. We compiled stellar spectra from 31 old and intermediate-age open
clusters, homogeneously derived atmospheric parameters, and 17 abundance
species, and applied machine learning algorithms to group the stars based on
their chemical composition. This approach allows us to evaluate the viability
and efficiency of the chemical tagging technique.
Results. We found that stars at different evolutionary stages have distinct
chemical patterns that may be due to NLTE effects, atomic diffusion, mixing,
and biases. When separating stars into dwarfs and giants, we observed that a
few open clusters show distinct chemical signatures while the majority show a
high degree of overlap. This limits the recovery of co-natal aggregates by
applying the chemical tagging technique. Nevertheless, there is room for
improvement if more elements are included and models are improved.Comment: accepted for publication in Astronomy and Astrophysics. Corrected
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The M33 Globular Cluster System with PAndAS Data: The Last Outer Halo Cluster?
We use CFHT/MegaCam data to search for outer halo star clusters in M33 as
part of the Pan-Andromeda Archaeological Survey (PAndAS). This work extends
previous studies out to a projected radius of 50 kpc and covers over 40 square
degrees. We find only one new unambiguous star cluster in addition to the five
previously known in the M33 outer halo (10 kpc <= r <= 50 kpc). Although we
identify 2440 cluster candidates of various degrees of confidence from our
objective image search procedure, almost all of these are likely background
contaminants, mostly faint unresolved galaxies. We measure the luminosity,
color and structural parameters of the new cluster in addition to the five
previously-known outer halo clusters. At a projected radius of 22 kpc, the new
cluster is slightly smaller, fainter and redder than all but one of the other
outer halo clusters, and has g' ~ 19.9, (g'-i') ~ 0.6, concentration parameter
c ~ 1.0, a core radius r_c ~ 3.5 pc, and a half-light radius r_h ~ 5.5 pc. For
M33 to have so few outer halo clusters compared to M31 suggests either tidal
stripping of M33's outer halo clusters by M31, or a very different, much calmer
accretion history of M33.Comment: 37 pages, 9 figures. Accepted by the Astrophysical Journa
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