Iberian red deer: Paraphyletic nature at mtDNA but nuclear markers support its genetic identity

British Ecological Society Special Feature: Demography Beyond the Population.Red deer populations in the Iberian glacial refugium were the main source for postglacial recolonization and subspecific radiation in north-western Europe. However, the phylogenetic history of Iberian red deer (Cervus elaphus hispanicus) and its relationships with northern European populations remain uncertain. Here, we study DNA sequences at the mitochondrial control region along with STR markers for over 680 specimens from all the main red deer populations in Spain and other west European areas. Our results from mitochondrial and genomic DNA show contrasting patterns, likely related to the nature of these types of DNA markers and their specific processes of change over time. The results, taken together, bring support to two distinct, cryptic maternal lineages for Iberian red deer that predated the last glacial maximum and that have maintained geographically well differentiated until present. Haplotype relationships show that only one of them contributed to the northern postglacial recolonization. However, allele frequencies of nuclear markers evidenced one main differentiation between Iberian and northern European subspecies although also supported the structure of both matrilines within Iberia. Thus, our findings reveal a paraphyletic nature for Iberian red deer but also its genetic identity and differentiation with respect to northern subspecies. Finally, we suggest that maintaining the singularity of Iberian red deer requires preventing not only restocking practices with red deer specimens belonging to other European populations but also translocations between both Iberian lineages.This work was partly supported by project CGL2010-17163/BOS from the Spanish MInistry of Science and by Extremadura and Andalusian Regional Goverments.Peer Reviewe

Utilización conjunta de técnicas de inmovilización y fitoextracción en la descontaminación de suelos

Se estudia la viabilidad de la combinación de dos técnicas (estabilización y fitoextracción) a partir del estudio de los lixiviados y de las tasas de fitoextracción. Como enmienda estabilizante se usó lodo de mármol (~ 97% de CaCO3) y como planta fitoextractora Brassica juncea L. El estudio se llevó a cabo en dos suelos (uno ácido y otro básico) contaminados por As, Pb y Zn. La adición de lodo de mármol disminuyo significativamente la concentración de contaminantes en los lixiviados, al tiempo que la fitoextracción de dichos contaminantes siguió siendo relativamente elevada. Por tanto, la utilización conjunta de ambas técnicas reduce significativamente el lavado y dispersión de contaminantes al tiempo que sigue posibilitando su fitoextracción

Price strategies of independent and branded dealers in retail gas market : the case of a contract reform in Spain

Este documento analiza cómo la estructura contractual entre las estaciones de servicio y el operador mayorista afecta a las estrategias de precios. Utilizando los precios diarios de las estaciones de servicio, en el documento se observa que los distribuidores independientes cobran márgenes más bajos que otras estaciones de servicio con algún tipo de contrato en exclusividad con un operador al por mayor. Una explicación posible es que las estaciones independientes reaccionan más al número de competidores. Utilizamos la introducción de un impuesto autonómico especial discrecional a los carburantes (IVMDH o céntimo sanitario) para verificar la reacción de los márgenes a cambios en los costes marginales de los competidores. Los resultados son consistentes con la idea de que, independientemente del tipo de contrato, todos los distribuidores reaccionan notablemente a aumentos relativos de los costes marginales, disminuyendo, en promedio, los márgenes. Usamos esos resultados para interpretar la reducción inexistente de los márgenes que siguió al cambio en la regulación española que tuvo lugar en 2013 para fomentar la competencia en el sector minorista. Una posible interpretación es que para la mayoría de las estaciones de servicio el importante aumento de las estaciones independientes después de la reforma no se consideró un aumento de la competencia realThis paper analyses how the contract structure between gas stations and the wholesale operator affects price strategies. Using daily data on prices of different gas stations the paper finds that independent dealers charge lower margins than other dealers with different contracts. One potential hypothesis is that this is the case because independent stations react more to the number of competitors. We use the introduction of a discretional regional excise duty (IVMDH) on gas stations to check the reaction of markups to changes in marginal costs of the actual number of competitors. Results are consistent with the idea that regardless the type of contract all dealers react notably to the increases in relative marginal costs by decreasing average markups. We use those results to interpret the inexistent reduction in markups that followed a change in the Spanish regulation that took place in 2013 fostering competition in the retail sector. One potential interpretation is that the big increase in independent stations following the reform was not considered an increase in actual competition for most of the incumbent station

Prostatic cyst: An unusual cause of hemospermia

AbstractA 31-year-old man was referred to our service because of recurrent hemospermia over the last 4 years, there were no other symptoms like perineal pain, fever, dysuria, nocturia or urgency; this patient only presented hemospermia with clots. Genital examination was normal. Semen analysis showed no change in volume and pH; however, hemospermia and asthenozoospermia were observed. The semen culture was normal. Ultrasonography only revealed the presence of a cystic lesion adjoining the prostate gland, next to the bladder neck. The retrograde urethrogram was normal. The CT scan revealed only a small calcification in the right seminal vesicle. Endoscopic cistourethroscopy demonstrated 2 cystic dilatations arising on both sides of the prostate gland adjacent to the bladder neck, behind the verumontanum with vessels running over the surface prostatic cyst dilatation. The diagnosis of prostatic gland cystic dilatation was reached and confirmed by pathology that reported fibroconnective tissue with fibrosis and hyalinization. Transurethral unroofing of the cyst was performed separately with a successful outcome

Detection of blaCTX-M-15 in an integrative and conjugative element in four extensively drug-resistant Haemophilus parainfluenzae strains causing urethritis

Haemophilus parainfluenzae is a commensal organism with rising numbers of multidrug-resistant (MDR) strains. This pathogen is of increasing clinical relevance in urogenital infection. The aim of this work was to identify and characterise the molecular mechanisms of resistance associated with four cephalosporin-resistant H. parainfluenzae strains collected from patients with urethritis. Antimicrobial resistance was determined by microdilution following European Committee on Antimicrobial Susceptibility Testing cri-teria. Strains were then analysed by whole-genome sequencing to determine clonal relationship and the molecular basis of antimicrobial resistance. Finally, a phylogenetic analysis was performed on all urogen-ital MDR strains of H. parainfluenzae previously isolated in our hospital. All strains were resistant to ,B- lactams, macrolides, tetracycline, fluoroquinolones, chloramphenicol, cotrimoxazole, and aminoglycosides. The resistance profile was compatible with the presence of an extended-spectrum ,B-lactamase (ESBL). Whole-genome sequencing detected blaCTX-M-15 that conferred high minimum inhibitory concentrations to cephalosporins in two novel integrative and conjugative elements (ICEHpaHUB6 and ICEHpaHUB7) that also harboured a blaTEM-1 ,B-lactamase. This study shows a novel bla CTX-M-15 ESBL carried in an integrative conjugative element in four extensively drug-resistant H. parainfluenzae strains. This resistance determi-nant could be transmitted to other sexually transmitted pathogens and this is a cause for concern. (c) 2023 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/

Valproic acid restricts mast cell activation by Listeria monocytogenes.

Mast cells (MC) play a central role in the early containment of bacterial infections, such as that caused by Listeria monocytogenes (L.m). The mechanisms of MC activation induced by L.m infection are well known, so it is possible to evaluate whether they are susceptible to targeting and modulation by different drugs. Recent evidence indicates that valproic acid (VPA) inhibits the immune response which favors L.m pathogenesis in vivo. Herein, we examined the immunomodulatory effect of VPA on L.m-mediated MC activation. To this end, bone marrow-derived mast cells (BMMC) were pre-incubated with VPA and then stimulated with L.m. We found that VPA reduced MC degranulation and cytokine release induced by L.m. MC activation during L.m infection relies on Toll-Like Receptor 2 (TLR2) engagement, however VPA treatment did not affect MC TLR2 cell surface expression. Moreover, VPA was able to decrease MC activation by the classic TLR2 ligands, peptidoglycan and lipopeptide Pam3CSK4. VPA also reduced cytokine production in response to Listeriolysin O (LLO), which activates MC by a TLR2-independent mechanism. In addition, VPA decreased the activation of critical events on MC signaling cascades, such as the increase on intracellular Ca2+ and phosphorylation of p38, ERK1/2 and -p65 subunit of NF-κB. Altogether, our data demonstrate that VPA affects key cell signaling events that regulate MC activation following L.m infection. These results indicate that VPA can modulate the functional activity of different immune cells that participate in the control of L.m infection

Conflictos socioambientales y alternativas de la sociedad civil

Los conflictos socioambientales se multiplican en todo el mundo. Giran en torno a la contaminación industrial, las actividades mineras, el cambio de uso de suelo, la deforestación, la construcción de presas, la introducción de semillas genéticamente modificadas, el mal manejo de desechos sólidos y la privatización de tierra, agua y biodiversidad, entre otros. ¿Cuáles son las causas subyacentes a estos conflictos? ¿Quiénes son los protagonistas? ¿Cuáles son sus demandas, propuestas y estrategias? ¿En qué medida han contribuido a proteger o sanear el medio ambiente? ¿Hay conflictos locales que se convierten en glocales con la intervención de la sociedad civil? Estas interrogantes se abordan en este libro, que recoge inventarios de conflictos ambientales en México, en especial en Jalisco.ITESO, A.C

Standard comparison of local mental health care systems in eight European countries

Aims. There is a need of more quantitative standardised data to compare local Mental Health Systems (MHSs) across international jurisdictions. Problems related to terminological variability and commensurability in the evaluation of services hamper like-with-like comparisons and hinder the development of work in this area. This study was aimed to provide standard assessment and comparison of MHS in selected local areas in Europe, contributing to a better understanding of MHS and related allocation of resources at local level and to lessen the scarcity in standard service comparison in Europe. This study is part of the Seventh Framework programme REFINEMENT (Research on Financing Systems’ Effect on the Quality of Mental Health Care in Europe) project. Methods. A total of eight study areas from European countries with different systems of care (Austria, England, Finland, France, Italy, Norway, Romania, Spain) were analysed using a standard open-access classification system (Description and Evaluation of Services for Long Term Care in Europe, DESDE-LTC). All publicly funded services universally accessible to adults (≥18 years) with a psychiatric disorder were coded. Care availability, diversity and capacity were compared across these eight local MHS. Results. The comparison of MHS revealed more community-oriented delivery systems in the areas of England (Hampshire) and Southern European countries (Verona – Italy and Girona – Spain). Community-oriented systems with a higher proportion of hospital care were identified in Austria (Industrieviertel) and Scandinavian countries (Sør-Trøndelag in Norway and Helsinki-Uusimaa in Finland), while Loiret (France) was considered as a predominantly hospital-based system. The MHS in Suceava (Romania) was still in transition to community care. Conclusions. There is a significant variation in care availability and capacity across MHS of local areas in Europe. This information is relevant for understanding the process of implementation of community-oriented mental health care in local areas. Standard comparison of care provision in local areas is important for context analysis and policy planning

A New Measurement of the 1S0 Neutron-Neutron Scattering Length using the Neutron-Proton Scattering Length as a Standard

The present paper reports high-accuracy cross-section data for the 2H(n,nnp) reaction in the neutron-proton (np) and neutron-neutron (nn) final-state-interaction (FSI) regions at an incident mean neutron energy of 13.0 MeV. These data were analyzed with rigorous three-nucleon calculations to determine the 1S0 np and nn scattering lengths, a_np and a_nn. Our results are a_nn = -18.7 +/- 0.6 fm and a_np = -23.5 +/- 0.8 fm. Since our value for a_np obtained from neutron-deuteron (nd) breakup agrees with that from free np scattering, we conclude that our investigation of the nn FSI done simultaneously and under identical conditions gives the correct value for a_nn. Our value for a_nn is in agreement with that obtained in pion-deuteron capture measurements but disagrees with values obtained from earlier nd breakup studies.Comment: 4 pages and 3 figure