Nox1/4 inhibition exacerbates age dependent perivascular inflammation and fibrosis in a model of spontaneous hypertension

Hypertension is associated with oxidative stress and perivascular inflammation, critical contributors to perivascular fibrosis and accelerated vascular ageing. Oxidative stress can promote vascular inflammation, creating options for potential use of NADPH oxidase inhibitors in pharmacological targeting of perivascular inflammation and its consequences. Accordingly, we characterized age-related changes in oxidative stress and immune cell infiltration in normotensive (WKY) and spontaneously hypertensive rats (SHRs). Subsequently, we used pharmacological inhibitors of Nox1 (ML171) and Nox1/Nox4 (GKT137831; 60 mg/kg), to modulate NADPH oxidase activity at the early stage of spontaneous hypertension and investigated their effects on perivascular inflammation and fibrosis. Results: Ageing was associated with a progressive increase of blood pressure as well as an elevation of the total number of leukocytes, macrophages and NK cells infiltrating perivascular adipose tissue (PVAT) in SHRs but not in WKY. At 1 month of age, when blood pressure was not yet different, only perivascular NK cells were significantly higher in SHR. Spontaneous hypertension was also accompanied by the higher perivascular T cell accumulation, although this increase was age independent. Aortic Nox1 and Nox2 mRNA expression increased with age only in SHR but not in WKY, while age-related increase of Nox4 mRNA in the vessels has been observed in both groups, it was more pronounced in SHRs. At early stage of hypertension (3-months) the most pronounced differences were observed in Nox1 and Nox4. Surprisingly, GKT137831, dual inhibitor of Nox1/4, therapy increased both blood pressure and perivascular macrophage infiltration. Mechanistically, this was linked to increased expression of proinflammatory chemokines expression (CCL2 and CCL5) in PVAT. This inflammatory response translated to increased perivascular fibrosis. This effect was likely Nox4 dependent as the Nox1 inhibitor ML171 did not affect the development of spontaneous hypertension, perivascular macrophage accumulation, chemokine expression nor adventitial collagen deposition. In summary, spontaneous hypertension promotes ageing-associated perivascular inflammation which is exacerbated by Nox4 but not Nox1 pharmacological inhibition

Chrysoperla carnea Stephens (Chrysopidae: Neuroptera) - a potential biocontrol agent of cardamom aphid (Pentalonia nigronervosa f. caladii van der Goot)

Viral diseases such as mosaic (katte) and vein clearing disease (kokke kandu) of cardamom (Elettaria cardamomum Maton) are transmitted by the cardamom aphid (Pentalonia nigronervosa f. caladii van der Goot) (Homoptera: Aphididae). Insecticides are ineffective in controlling the aphids since their colonies are found in the concealed senile parts of the host and also due to their hyperactivity and subsequent migration and probing to other plants on exposure to insecticides (Raj an et al. 1989) which might further increase the spread of the disease. &nbsp

Chrysoperla carnea Stephens (Chrysopidae: Neuroptera) - a potential biocontrol agent of cardamom aphid (Pentalonia nigronervosa f. caladii van der Goot)

Viral diseases such as mosaic (katte) and vein clearing disease (kokke kandu) of cardamom (Elettaria cardamomum Maton) are transmitted by the cardamom aphid (Pentalonia nigronervosa f. caladii van der Goot) (Homoptera: Aphididae). Insecticides are ineffective in controlling the aphids since their colonies are found in the concealed senile parts of the host and also due to their hyperactivity and subsequent migration and probing to other plants on exposure to insecticides (Raj an et al. 1989) which might further increase the spread of the disease. &nbsp

Efficacy of entomogenous fungi on biological suppression of Pentalonia nigronervosa f. caladii Van der Goot of cardamom (Elettaria cardamomum Maton)

Experiments were conducted in the laboratory on the biological suppression of the aphid Pentalonia nigronervosa f. caladii of cardamom (Elettaria cardamomum) using four entomogenous fungi namely, Beauveria bassiana, B. brongniartii, Verticillium chlamydosporium and Metarhizium anisopliae. Fungal spore suspensions (1 x 107 spores/ml) when applied on the aphid colonies caused mortality to both apterous adults and nymphs ranging from 37.0 to 96.6 per cent and 32.S to 75.4 per cent, respectively. B. bassiana was the most potent biocontrol agent effecting 96.6 and 75.4 per cent mortality for adults and nymphs, respectively, in the indirect expsoure method and 65.9 and 70.9 per cent, respectively, in the direct exposure method. &nbsp

Different types of Corrective exercises on Correction of Hyper Lumbarlordosis in Females- A Narrative Review

Back ground: Lumbar curvature carries the upper body weight and transfers it directly to the pelvis, which is of great significance. The structures in the lumbar region are one of the factors that affect the lumbar-pelvic balance, as well as the performance of lumbar lordosis and pelvic tilt. Also, weakness of abdominal, dorsal, and lumbar muscles has been considered as the most common factors increasing the lumbar curvature. Excessive lordotic curvature is also called hyperlordosis, hollow back, saddle back and swayback. Common cause of excessive lordosis includes tight low back muscle, excessive visceral fat, and pregnancy. Objectives: This review aimed at finding and analysing different forms of corrective exercises to correct hyper lumbar lordosis in females. Methods: The search was performed through online for English language articles. The databases used were Google ‘corrective exercises’ and ‘lumbar lordosis’. The scientific literature related to physiotherapy management for lumbar lordosis published from 1997 to 2021 was searched. Screening of the reference lists of all the retrieved articles was also done. Through online database search 100 articles were reviewed, 19 articles were included in this study based on predetermined inclusion criteria. Inclusion criteria: Based on gender (only female’s participant’s studies). Participants: Studies had included individuals with hyper lumbar lordosis with pain or without low back pain. Conclusion: 16 articles show Corrective exercises plays a main role in reduction of lumbar lordotic curve and functional disability. With the help of corrective exercises (abdominal muscle strengthening exercises, gluteal strengthening exercises hip flexor stretching exercises, back stretching exercises) can maintain the core stability of spinal extensors and flexors, due to this, spinal curvature can be corrected indirectly and the posture is corrected and the spasm get released, pain will be subsided, finally the quality of life will be improved

Nox1/4 inhibition exacerbates age dependent perivascular inflammation and fibrosis in a model of spontaneous hypertension

Hypertension is associated with oxidative stress and perivascular inflammation, critical contributors to perivascular fibrosis and accelerated vascular ageing. Oxidative stress can promote vascular inflammation, creating options for potential use of NADPH oxidase inhibitors in pharmacological targeting of perivascular inflammation and its consequences. Accordingly, we characterized age-related changes in oxidative stress and immune cell infiltration in normotensive (WKY) and spontaneously hypertensive rats (SHRs). Subsequently, we used pharmacological inhibitors of Nox1 (ML171) and Nox1/Nox4 (GKT137831; 60 mg/kg), to modulate NADPH oxidase activity at the early stage of spontaneous hypertension and investigated their effects on perivascular inflammation and fibrosis. Results: Ageing was associated with a progressive increase of blood pressure as well as an elevation of the total number of leukocytes, macrophages and NK cells infiltrating perivascular adipose tissue (PVAT) in SHRs but not in WKY. At 1 month of age, when blood pressure was not yet different, only perivascular NK cells were significantly higher in SHR. Spontaneous hypertension was also accompanied by the higher perivascular T cell accumulation, although this increase was age independent. Aortic Nox1 and Nox2 mRNA expression increased with age only in SHR but not in WKY, while age-related increase of Nox4 mRNA in the vessels has been observed in both groups, it was more pronounced in SHRs. At early stage of hypertension (3-months) the most pronounced differences were observed in Nox1 and Nox4. Surprisingly, GKT137831, dual inhibitor of Nox1/4, therapy increased both blood pressure and perivascular macrophage infiltration. Mechanistically, this was linked to increased expression of proinflammatory chemokines expression (CCL2 and CCL5) in PVAT. This inflammatory response translated to increased perivascular fibrosis. This effect was likely Nox4 dependent as the Nox1 inhibitor ML171 did not affect the development of spontaneous hypertension, perivascular macrophage accumulation, chemokine expression nor adventitial collagen deposition. In summary, spontaneous hypertension promotes ageing-associated perivascular inflammation which is exacerbated by Nox4 but not Nox1 pharmacological inhibition

Nutrigenomic and nutritional analyses reveal the effects of pelleted feeds on Asian Seabass (Lates calcarifer)

As nutrition-related expenses constitute the majority of the costs for aquaculture farms, it is essential for them to use feeds that provide an ideal combination of nutrients for the species of choice. In this study, the relative effect of consuming three different pelleted feeds (B, C and D) in comparison to frozen baitfish (A; control) were compared on juvenile Asian seabass (77.3 ± 22.4g) that were selected for increased growth rate over two generations. Our objectives were: 1) to evaluate the effects of different pelleted feeds based on overall physiological changes and nutritional quality of fillets; 2) improve our understanding of the underlying mechanisms with transcriptomic analysis; 3) if possible, identify the feed type that supports the growth of these fishes without substantially reducing the nutritional quality of fillet. The growth performance, fatty acid composition of fillet, hepatic histology and transcriptome of the fishes (Groups A-D) were analyzed. The majority of fatty acids of the fillets, except γ-linolenic acid (GLA, C18:3n6), correlated significantly with the respective diets. Asian seabass fed Feed C showed highest specific growth rate (SGR) and feed conversion efficiency (FCE) with closest histology and transcriptomic profile to control, but their fillet contained the highest n6/n3 ratio. When the liver-based transcriptomes were analyzed, a complex set of differentially expressed genes were detected between groups fed pelleted feeds and controls as well as among the pellet-fed groups themselves. Significant enrichment of genes with growth-related function tallied with the morphological data measured. When compared with control (Group A), ‘Biosynthesis of unsaturated fatty acids’ and ‘Steroid biosynthesis’ pathways were significantly enriched in pellet-fed groups. Reduced goblet cell numbers were observed in the gut of pellet-fed fish compared to controls and fads6 was found to be a suitable candidate gene to separate wild-caught Asian seabass, from pellet-fed ones. These results provide insights for researchers on the various effects of feeds on the biochemistry and global gene expression of the fish and potentially for seabass farms to make more informed feed choices

Barcoding of Asian seabass across its geographic range provides evidence for its bifurcation into two distinct species

Asian seabass or barramundi (Lates calcarifer) is an important food fish with commercial value and a wide geographic distribution. Though some reports based on molecular and/or morphological data exist, a comprehensive effort to establish species identity across its range is lacking. In order to address this issue and especially to ascertain whether the wide-spread distribution has resulted in bifurcation of the species, we collected Asian seabass samples from various locations representing the Western and Eastern Coastline of India, Andaman and Nicobar Islands, Bangladesh and Australia. Samples from Malaysia, Indonesia, Thailand and Singapore were collected as part of a previous study. DNA sequence variations, including cytochrome c oxidase subunit 1 (COI), 16S rDNA and the highly variable D-loop (or control region), were examined to establish species delineation. Data from all the sequences analyzed concordantly point to the existence of at least two distinct species—one representing the Indian subcontinent plus Myanmar, and a second, representing Southeast Asia (Singapore, Malaysia, Thailand and Indonesia) plus Northern Australia. These data are useful for conservation ecology, aquaculture management, for establishing the extent of genetic diversity in the Asian seabass and implementing selective breeding programs for members of this species complex

Identity of Colletotrichum infections in large cardamom (Amomum subulatum Roxb.)

The pathogen causing blight and severe devastation of large cardamom in Sikkim and Darjeeling Hills was isolated and identified as Colletotrichum gloeosporioides (Penz.) Sacc. {perfect state Glomerella cingulata (Stoneman) Spauld. & Schrenk}. We propose the name, Colletotrichum blight for the disease. &nbsp

Chromosomal-level assembly of the Asian Seabass genome using long sequence reads and multi-layered scaffolding

We report here the ~670 Mb genome assembly of the Asian seabass (Lates calcarifer), a tropical marine teleost. We used long-read sequencing augmented by transcriptomics, optical and genetic mapping along with shared synteny from closely related fish species to derive a chromosome-level assembly with a contig N50 size over 1 Mb and scaffold N50 size over 25 Mb that span ~90% of the genome. The population structure of L. calcarifer species complex was analyzed by re-sequencing 61 individuals representing various regions across the species' native range. SNP analyses identified high levels of genetic diversity and confirmed earlier indications of a population stratification comprising three clades with signs of admixture apparent in the South-East Asian population. The quality of the Asian seabass genome assembly far exceeds that of any other fish species, and will serve as a new standard for fish genomics