Evaluation of anti-cancer effect of Peganum harmala L hydroalcholic extract on human cervical carcinoma epithelial cell line

زمینه و هدف: سرطان دهانه رحم دومین سرطان شایع در زنان می باشد. اسپند گیاهی طبی بوده و یکی از فراورده های بومی به کار رفته جهت درمان سرطان در ایران می باشد. این گیاه دارای خواص ضد باکتریایی، ضد سرطانی، ضد قارچ، ضد زخم معده می باشد. این مطالعه با هدف بررسی اثر ضد سرطانی عصاره ی هیدروالکلی دانه گیاه اسپند انجام شده است. روش بررسی: در این مطالعه تجربی پس از کشت و تکثیر سلول های مشتق شده از بافت اپیتلیال گردن رحم انسان (HeLa)، این سلول ها در مجاورت دوزهای مختلف عصاره هیدروالکلی دانه اسپند (5/12،25،50 و g/mlµ 100) قرار گرفتند و به مدت 24،48 و 72 ساعت انکوبه شدند. پس از پایان مدت انکوباسیون، از روش تغییر یافته آزمون رنگ سنجی MTT (3- (4 و 5 دی متیل تیازول 2- ایل)-2،5 - دی فنیل تترازولیوم برماید) جهت تعیین سمیت سلولی عصاره استفاده شد. یافته ها: نتایج حاصل از تست MTT نشان داد که این عصاره اثر ضد سرطانی وابسته به دوز و زمان بر سلول های HeLa دارد، به طوری که با افزایش غلظت عصاره و انکوباسیون 72 ساعت بیشترین درصد مرگ سلولی مشاهده شد (001/0>P). غلظت مهارکنندگی 50 درصد رشد سلول ها (IC50) برای سلول های سرطانی در زمان 24 ساعت g/mlµ 5/12 به دست آمد. نتیجه گیری: عصاره اسپند با اثر وابسته به دوز و زمان بر سلول های سرطانی HeLa می تواند باعث مهار رشد این سلول ها شود؛ لذا به نظر می رسد با تحقیقات بیشتر در آینده، می توان از ترکیبات آن در درمان سرطان بهره جست

Aromatherapy with Rosa Damascenes in Apnea, Bradycardia and Spo2 of Preterm Infants; a Randomized Clinical Trial

Background  Apnea is one of the most common problems in preterm neonates. This study aimed to evaluate the effectiveness of aromatherapy on the reduction of apnea, bradycardia, and Pulse Oximetry (SPO2) in premature infants. Materials and Methods In a clinical trial study, 60 preterm neonates randomly divided in two groups and exposed to aromatherapy with Rosa damascenes distillate or distilled water beside the routine treatment. In experimental group, two drops of 10% Rosa damascenes distillate was dropped on the pad eye in second day of birth at 6 Am. The intervention was repeated in 9 AM, 12 Am, 15 PM and 18 PM hours. A same condition applied for control group and distilled water was dropped on the pad eye. The number of apnea attacks, bradycardia and decrease in SpO2 compared between two groups using chi-square test, independent t-test and repeated measure test. Results The overall apnea attacks, bradycardia, and SPO2 in tree studied days were lower in intervention group than control group (0.47±0.13 vs. 2.6±0.41, 0.47±0.13 vs. 2.56±0.41 and 0.70±0.17 vs. 2.77±0.21, respectively). In addition, the repeated measurement test showed that the mean number of apnea attacks, decrease in heart pulse rate, and decrease in SpO2 was statistically lower in intervention group with aromatherapy than controls in first, second, third and sum of three days (P< 0.05). Conclusion Aromatherapy with Rosa damascenes distillate can reduce more and speedy the occurrence of apnea attacks, bradycardia and SPO2 in premature infants, along with other routine treatment

Ranking of Zahedan’s Five Districts in Order to Fulfill the Creative City

Moving towards the development and realization of the creative city due to the status of the city as a place of forming knowledge society contexts, and the importance of cities in economic development is very necessary and important. This paper aims to examine the components of the creative city in Zahedan and tries to move toward urban creativity. Considering the components of this research, it is an applied study, which is conducted through a descriptive-analytical method. The research includes 20 indicators for the creative city. A researcher made questionnaire is used to collect data. In addition, SPSS and GIS softwares are used to analyze the data. The statistical population is the five districts of Zahedan City, in which 383 residents were selected and studied through cluster and systematic random sampling in all districts of the city. The ranking results of districts in the creative city indicators show that district 1 is the most desirable district and district 3 is the most undesirable and the most deprived district with informal and disturbed settlements. The effectiveness of each component of the creative city indicators was studied in the regression analysis. It was found that the effectiveness of all components is not identical in the realization of the creative city in Zahedan. They act in the form of a chain and the stability of the chain depends on the planning and investment in all of the sub-indicators of these components

Therapeutic potential of hair follicle-derived stem cell intranasal transplantation in a rat model of ischemic stroke

Background Stem cell-based therapy has received considerable attention as a potential candidate in the treatment of ischemic stroke; however, employing an appropriate type of stem cells and an effective delivery route are still challenging. In the present study, we investigated the therapeutic effect of safe, noninvasive, and brain-targeted intranasal administration of hair follicle-derived stem cells (HFSCs) in a rat model of ischemic stroke. Methods Stem cells were obtained from the adult rat hair follicles. In experiment 1, stroke was induced by 30 min middle cerebral artery occlusion (MCAO) and stem cells were intranasally transplanted immediately after ischemia. In experiment 2, stroke was induced by 120 min MCAO and stem cells were administered 24 h after cerebral ischemia. In all experimental groups, neurological performance, short-term spatial working memory and infarct volume were assessed. Moreover, relative expression of major trophic factors in the striatum and cortex was evaluated by the quantitative PCR technique. The end point of experiment 1 was day 3 and the end point of experiment 2 was day 15. Results In both experiments, intranasal administration of HFSCs improved functional performance and decreased infarct volume compared to the MCAO rats. Furthermore, NeuN and VEGF expression were higher in the transplanted group and stem cell therapy partially prevented BDNF and neurotrophin-3 over-expression induced by cerebral ischemia. Conclusions These findings highlight the curative potential of HFSCs following intranasal transplantation in a rat model of ischemic stroke.UCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologí

Egg consumption and risk of cardiovascular disease: a PERSIAN cohort-based study

Abstract Background Cardiovascular diseases (CVDs) are one of the main causes of death worldwide. Data on the effect of eggs consumption on the risk of CVDs are still unreliable. Therefore, this study aimed to investigate the association between CVDs and the consumption of eggs. Methods In this case-control study, the required data were extracted from the Prospective Epidemiologic Research Studies in Iran (PERSIAN) cohort in Sabzevar, Iran. A total of 4241 adults participated including 1535 patients with CVDs as the case group and 2706 healthy people as controls. Egg consumption was assessed using a valid food frequency questionnaire (FFQ). Results A significant association was observed between eggs consumption and stroke after adjustment for physical activity, body mass index (BMI), smoking, systolic blood pressure (SBP), diastolic blood pressure (DBP), using alcohol, lipid profile, diabetes, and the intake of energy, lipid, protein, carbohydrate, and cholestrol (OR:1.007, 95% CI:1.001–1.013, P = 0.03). No association was found between egg consumption with hypertension, cardiac ischemia, and myocardial infarction. Conclusions There was a significant association between the consumption of eggs and stroke. However, more studies are needed to examine the effect of eggs on CVDs