Nanoplastics: From tissue accumulation to cell translocation into Mytilus galloprovincialis hemocytes. resilience of immune cells exposed to nanoplastics and nanoplastics plus Vibrio splendidus combination

Plastic litter is an issue of global concern. In this work Mytilus galloprovincialis was used to study the distribution and effects of polystyrene nanoplastics (PS NPs) of different sizes (50 nm, 100 nm and 1 mu m) on immune cells. Internalization and translocation of NPs to hemolymph were carried out by in vivo experiments, while endocytic routes and effects of PS NPs on hemocytes were studied in vitro. The smallest PS NPs tested were detected in the digestive gland and muscle. A fast and size-dependent translocation of PS NPs to the hemolymph was recorded after 3 h of exposure. The internalization rate of 50 nm PS NPs was lower when caveolae and clathrin endocytosis pathways were inhibited. On the other hand, the internalization of larger particles decreased when phagocytosis was inhibited. The hemocytes exposed to NPs had changes in motility, apoptosis, ROS and phagocytic capacity. However, they showed resilience when were infected with bacteria after PS NP exposure being able to recover their phagocytic capacity although the expression of the antimicrobial peptide Myticin C was reduced. Our findings show for the first time the translocation of PS NPs into hemocytes and how their effects trigger the loss of its functional parameters

Eco-geomorphology and vegetation patterns in arid and semi-arid regions

International audienceThe interaction between vegetation and hydrologic processes is particularly tight in water-limited environments where a positive-feedback links water redistribution and vegetation. The vegetation of these systems is commonly patterned, that is, arranged in a two phase mosaic composed of patches with high biomass cover interspersed within a low-cover or bare soil component. These patterns are strongly linked to the redistribution of runoff and resources from source areas (bare patches) to sink areas (vegetation patches) and play an important role in controlling erosion. In this paper a new modeling framework that couples landform evolution and dynamic vegetation for water-limited ecosystems is presented. The model explicitly accounts for the dynamics of runon-runoff areas that controls the evolution of vegetation and erosion/deposition patterns in water limited ecosystems. The analysis presented here focuses on the interaction between vegetation patterns, flow dynamics and sediment redistribution for areas with mild slopes where sheet flow occurs and banded vegetation patterns emerge. Model results successfully reproduce the dynamics of both migrating and stationary banded vegetation patterns (commonly known as tiger bush). Modeling results show strong feedbacks effects between vegetation patterns, runoff redistribution and geomorphic changes. The success at generating not only the observed patterns of vegetation but also patterns of runoff and erosion redistribution, which gives rise to modeled microtopography similar to that observed in several field sites, suggests that the hydrologic and erosion mechanisms represented in the model are correctly capturing the essential processes driving these ecosystems

Eco-geomorphology of banded vegetation patterns in arid and semi-arid regions

This study utilizes Robert Putnam’s “Two Level Game Theory” to understand whether public opinion influenced President Obama\u27s first-term administration when confronted with foreign policy decisions. Some scholars argue that there is an overall lack in understanding of how public opinion affects American foreign policy because the public is disengaged and uneducated on foreign issues and that the role of media influences the public’s attitude towards a certain issue. Accordingly, it is hypothesized here that public opinion was not a factor in this process. To test this proposition, the study evaluates three cases: the “closing” of Guantanamo Bay, the United States’ intervention in Libya, and the continuation of the drone program. Throughout each case, multiple variables are examined including: public opinion of the citizens and the political elite, President Obama’s political communication, and international diplomatic actions. Findings indicate that public opinion did not play any major role in President Obama’s foreign policy decision-making process; therefore, President Obama’s policies did not meet Robert Putnam’s criteria of domestic influence in the two level game theory

Assessing landscape structure and pattern fragmentation in semiarid ecosystems using patch-size distributions

Spatial vegetation patterns are recognized as sources of valuable information that can be used to infer the state and functionality of semiarid ecosystems, particularly in the context of both climate and land use change. Recent studies have suggested that the patch‐size distribution of vegetation in drylands can be described using power‐law metrics, and that these scale‐free distributions deviate from power‐law linearity with characteristic scale lengths under the effects of increasing aridity or human disturbance, providing an early sign of desertification. These findings have been questioned by several modeling approaches, which have identified the presence of characteristic scale lengths on the patch‐size distribution of semiarid periodic landscapes. We analyze the relationship between fragmentation of vegetation patterns and their patch‐size distributions in semiarid landscapes showing different degree of periodicity (i.e., banding). Our assessment is based on the study of vegetation patterns derived from remote sensing in a series of semiarid Australian Mulga shrublands subjected to different disturbance levels. We use the patch‐size probability density and cumulative probability distribution functions from both nondirectional and downslope analyses of the vegetation patterns. Our results indicate that the shape of the patch‐size distribution of vegetation changes with the methodology of analysis applied and specific landscape traits, breaking the universal applicability of the power‐law metrics. Characteristic scale lengths are detected in (quasi) periodic banded ecosystems when the methodology of analysis accounts for critical landscape anisotropies, using downslope transects in the direction of flow paths. In addition, a common signal of fragmentation is observed: the largest vegetation patches become increasingly less abundant under the effects of disturbance. This effect also explains deviations from power‐law behavior in disturbed vegetation which originally showed scale‐free patterns. Overall, our results emphasize the complexity of structure assessment in dryland ecosystems, while recognizing the usefulness of the patch‐size distribution of vegetation for monitoring semiarid ecosystems, especially through the cumulative probability distributions, which showed high sensitivity to fragmentation of the vegetation patterns. We suggest that preserving large vegetation patches is a critical task for the maintenance of the ecosystem structure and functionality

Diferenciación de cepas de Staphylococcus intermedius aisladas de perros con otitis externa

Se ha empleado la electroforesis en campo pulsado (PFGE) para diferernciar cepas de Staphylococcus intermedius aisladas de perros con otitis externa. De las 36 cepas analizadas se han obtenido 32 perfiles distintos. Estos resultados nos indican que la PFGE puede emplearse para diferenciar cepas de una misma especie

NDVI Response to Satellite-Estimated Antecedent Precipitation in Dryland Pastures

Precipitation is a critical driver of vegetation productivity and dynamics in dryland environments, especially in areas with intense livestock farming. Availability and access to accurate, reliable, and timely rainfall data are essential for natural resources management, environmental monitoring, and informing hydrological rainfall-runoff models. Gauged precipitation data in drylands are often scarce, fragmented, and with low spatial resolution; therefore, satellite-estimated precipitation becomes a valuable dataset for overcoming this constraint. Using statistical indices, we compared satellite-derived precipitation data from four products (CHIRPS, GPM, TRMM, and PERSIANN-CDR) against gauged data at different temporal scales (daily, monthly, and yearly). Spatial correlations were calculated for GPM and CHIRPS estimates against interpolated gauged precipitation. We then estimated NDVI response to Antecedent Accumulated Precipitation (AAP) for 1, 3, 6, 9, and 12 months of four major vegetation types typical of the region. Statistical metrics varied with temporal scales being highest and acceptable for periods of 1 month or 1 year. At monthly scale GPM presented the best Pearson’s Correlation Coefficient (r), Root Mean Square Error (RMSE) and RMSE-observations standard deviation ratio (RSR) and CHIRPS resulted in lower Mean Error (ME) and Bias. On an annual basis CHIRPS showed the best adjustment for all indicators except for r. NDVI responses to 3 months of AAP were significant for all vegetation types in the study area. The findings of this study show that estimated precipitation data from GPM and CHIRPS satellites are accurate and valuable as a tool for analysing the relationships between precipitation and vegetation in the drylands of MendozaEEA Rama CaídaFil: Brieva, Carlos. University of Newcastle. School of Engineering. Centre for Water Security and Environmental Sustainability; AustraliaFil: Brieva, Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Saco, Patricia, M. University of Newcastle. School of Engineering. Centre for Water Security and Environmental Sustainability; AustraliaFil: Sandi, Steven G. University of Newcastle. School of Engineering. Centre for Water Security and Environmental Sustainability; AustraliaFil: Sandi, Steven G. Deakin University. School of Engineering; AustraliaFil: Mora, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Rodríguez, José F. University of Newcastle. School of Engineering. Centre for Water Security and Environmental Sustainability; Australi

Study of urgent visits to commercial rabbit farms in Spain and Portugal during 1997-2007

[EN] This is a report on work carried out on 4307 visits to 868 commercial farms with domestic rabbits in Spain and Portugal from January 1997 to December 2007. Of the total visits, 2237 (52%) were emergencies on 660 farms. The median size of the farms ranged between 450 does in 1997 and 750 in 2007. This retrospective study measures the clinical disease occurrence using the Monthly Risk of urgent visits (MR), i.e. the percentage of visits made as a result of each clinical disease in comparison with the total number of urgent visits made each month. The main reasons for the emergencies were mucoid enteropathy (similar to Epizootic Rabbit Enteropathy), with a MR: 25.0%, enteritis-diarrhoea (24.1%), myxomatosis, (11.1%), reproductive troubles (8.6%), respiratory diseases (7.2%) and staphylococcosis (4.2%). Fifty-four percent of the urgent visits were due to diseases of the digestive system. Mucoid enteropathy was still one of the main diseases faced by the commercial rabbit industry during the study period. No significant yearly or monthly variations were observed in the analysis of the MR. A seasonal effect was only found in respiratory diseases during the summer (MR: 11.06±0.01) and myxomatosis in autumn (MR: 14.60±0.02), in comparison with spring (MR: 7.44±0.02). It is therefore concluded that farms should be permanently protected as they might be affected by any of these diseases at any time during the year.Rosell, J.; Fuente, LDL.; Badiola, J.; Fernández De Luco, D.; Casal, J.; Saco, M. (2009). Study of urgent visits to commercial rabbit farms in Spain and Portugal during 1997-2007. World Rabbit Science. 17(3):127-136. doi:10.4995/wrs.2009.65212713617

Structural and functional control of surface-patch to hillslope runoff and sediment connectivity in Mediterranean dry reclaimed slope systems

Connectivity has emerged as a useful concept for exploring the movement of water and sediments between landscape locations and across spatial scales. In this study, we examine the structural and functional controls of surfacepatch to hillslope runoff and sediment connectivity in three Mediterranean dry reclaimed mining slope systems that have different long-term development levels of vegetation and rill networks. Structural connectivity was assessed using flow path analysis of coupled vegetation distribution and surface topography, providing field indicators of the extent to which surface patches that facilitate runoff and sediment production are physically linked to one another in the studied hillslopes. Functional connectivity was calculated using the ratio of patch-scale to hillslope-scale observations of runoff and sediment yield for 21 monitored hydrologically active rainfall events. The impact of the dynamic interactions between rainfall conditions and structural connectivity on functional connectivity were further analysed using general linear models with a backward model structure selection approach. Functional runoff connectivity during precipitation events was found to be dynamically controlled by antecedent precipitation conditions and rainfall intensity and strongly modulated by the structural connectivity of the slopes. On slopes without rills, both runoff and sediments for all events were largely redistributed within the analysed hillslopes, resulting in low functional connectivity. Sediment connectivity increased with rainfall intensity, particularly in the presence of rill networks where active incision under high-intensity storm conditions led to large non-linear increases in sediment yield from the surface-patch to the hillslope scales. Overall, our results demonstrate the usefulness of applying structuraland functional-connectivity metrics for practical applications and for assessing the complex links and controlling factors that regulate the transference of both surface water and sediments across different landscape scales

Assessment of hydrocarbon pollution in NW Iberian Peninsula using bioaccumulation and molecular biomarkers in Mytilus galloprovincialis

Society of Environmental Toxicology and Chemistry - SETAC Europe 15th Annual Meeting, Lille, France, May 2005.Polycyclic aromatic hydrocarbons (PAH´s) are ubiquitous contaminants in marine environment as a result of uncontrolled spills, river transport, surface runoff and atmospheric deposition. A significant amount of industrial activity including shipping and oil refining is located along the NW Iberian Peninsula coast. The use of exposure biomarkers holds out promise due to the incipient state of the cost-effective methodologies for diagnosis and monitoring of oil pollution. This work presents the preliminary results concerning the identification of a set of biomarkers for an early warning detection of PAH toxicity. The bivalve Mytillus galloprovincialis was selected due to its ubiquitous distribution along coastline, being used as sentinels in pollution monitoring. This species has also an important value. Four locations in the vicinity of industrial wastewater discharges along the NW Iberian coast were selected and compared with a nearby (reference) site for (i) measurements of PAH body burdens and (ii) levels of enzyme activity: catalase (CAT), superoxide-dismutase (SOD), glutathione peroxidase (GPx), gluthathione S-transferase (GST) and Na+/K+ATPase (ATPase). The results will be discussed on the basis of their potential in providing additional evidence for discriminate between well known polluted and unpolluted sites