Effect of autolysis on the specificity of bovine spongiform encephalopathy rapid tests

<p>Abstract</p> <p>Background</p> <p>Routine rapid testing for Bovine Spongiform Encephalopathy (BSE) has highlighted some problems with BSE rapid test performance, the most significant being the number of initially reactive samples and the false positive results on autolyzed tissue. This point is important for BSE active surveillance in risk populations, because tissue autolysis is often unavoidable in routine cases. A robust test suitable for use on field material is therefore needed. To date, very limited information regarding the effect of autolysis on the robustness of rapid tests has been documented; therefore, the National Reference Centre for Animal Encephalopathies (CEA) rapid test laboratory selected 450 autolyzed and negative brain stem samples from fallen stock bovines older than 24 months to assess the specificity of four tests approved for BSE active surveillance: Biorad TeSeE, Enfer TSE version 2.0, Prionics^®Check LIA, and IDEXX Herd Check BSE Antigen Kit EIA. The samples were graded according to the degree of autolysis and then dissected into five portions, four of which randomly assigned to processing by rapid tests and one to be available for confirmatory Western blot analysis.</p> <p>Findings</p> <p>The specificity of the four systems was 100% for all three grades of autolysis, while the percentage of initially reactive results was 0.00 (95%CI 0.00-0.82), 0.22 (95%CI 0.006-1.23), 0.44 (95%CI 0.05-1.60), and 0.89 (95%CI 0.24-2.26) for the Biorad TeSeE, the Prionics^®Check LIA, the IDEXX Herd Check BSE and the Enfer TSE tests, respectively. No association with the degree of autolysis could be drawn.</p> <p>Conclusions</p> <p>The present study demonstrates that the four rapid tests can be considered well-running diagnostic tools regardless of tissue quality; nevertheless, the number of initial reactive samples reported for some systems must not be underestimated in routine testing.</p> <p>Furthermore the compliance with the reported performance can be guaranteed only when an ongoing high careful batch quality control system is in place.</p

Amplified immunohistochemical detection of PrPsc in animal transmissible spongiform encephalopathies using streptomycin.

International audienceDue to its sensitivity, immunohistochemistry (IHC) of abnormal prion protein (PrPsc) is used to study experimental and natural cases of transmissible spongiform encephalopathies (TSEs) such as Creutzfeldt-Jakob disease in humans or scrapie and bovine spongiform encephalopathy (BSE) in animals. The limits of detection are particularly critical when PrPsc IHC is used for diagnostic purposes. In this article, we describe for the first time the use of streptomycin sulfate in IHC, providing a novel original and easy way to amplify specifically PrPsc immunohistochemical detection in natural cases of BSE and scrapie, as well as in experimental TSEs in mice models using two different PrP antibodies.Due to its sensitivity, immunohistochemistry (IHC) of abnormal prion protein (PrPsc) is used to study experimental and natural cases of transmissible spongiform encephalopathies (TSEs) such as Creutzfeldt-Jakob disease in humans or scrapie and bovine spongiform encephalopathy (BSE) in animals. The limits of detection are particularly critical when PrPsc IHC is used for diagnostic purposes. In this article, we describe for the first time the use of streptomycin sulfate in IHC, providing a novel original and easy way to amplify specifically PrPsc immunohistochemical detection in natural cases of BSE and scrapie, as well as in experimental TSEs in mice models using two different PrP antibodies

The reaction e+ e- ---> gamma gamma (gamma) at Z0 energies

The total and differential cross-sections for the reaction e+e- → γγ(γ) are measured at centre of mass energies around 91 GeV using an integrated luminosity of 4.7 pb-1. The aggreement with QED prediction is good. Consequently there is no evidence for non-standard channels which would have the same experimental signature. The lower limits on the QED cuttoff parameters are Λ+ > 113 GeV and Λ- > 95 GeV. An upper limit on the effective coupling between a possible excited electron and the gamma is derived. At 95% confidence level the branching ratios for Z0 decay into π0γ, ηψ and γγγ are below 1.5 × 10-4, 2.8 × 10-4 and 1.4 × 10-4 respectively.0info:eu-repo/semantics/publishe

MULTIPLICITY DEPENDENCE OF MEAN TRANSVERSE-MOMENTUM IN E+E- ANNIHILATIONS AT LEP ENERGIES

A strong increase of the mean transverse momentum [p(t)] with the number of charged particles n(ch) is observed in e+e- annihilations into hadrons at LEP energies, The effect resembles correlations observed in hadron-hadron interactions. In e+e- annihilations the [p(t)] and n(ch) correlations can be accounted for by gluon radiation

A Measurement of sin**2 theta(W) from the charge asymmetry of hadronic events at the Z0 peak

Abstract View references (24)The weak mixing angle has been measured from the charge asymmetry of hadronic events with two different approaches using the DELPHI detector at LEP. Both methods are based on a momentum-weighted charge sum to determine the jet charge in both event hemispheres. In a data sample of 247 300 multihadronic Z0 decays a charge asymmetry of 〈QF〉 - 〈QB〉 = -0.0076±0.0012(stat.)±0.0005(exp. syst.)±0.0014(frag.) and a raw forward-backward asymmetry of Araw FB = -0.0109±0.0020(stat.)±0.0010(exp. syst.)±0.0017(frag.) have been measured. This result corresponds to a value of sinθeff=0.2345±0.0030(exp.)±0.0027(frag.) ,sin2θMS=0.2341±0.0030(exp.)±0. 0027(frag.) and to sin2θW=1-m2 W/m2 Z=0.2299± 0.0030(exp.)±0.0027(frag.)±0.0028(theor.). The experimental error is the quadratic sum of the statistical and the experimental systematic error and the theoretical error originates from a value of mt=130±40 GeV/c2 and a range of mH from 45 GeV/c2 to 1000 GeV/c2.0info:eu-repo/semantics/publishe