Slurry Acidification as a Solution to Minimize Ammonia Emissions from the Combined Application of Animal Manure and Synthetic Fertilizer in No-Tillage

The combined application of manure/slurry and synthetic fertilizer (SF) might be a solution to decrease transport and application costs involving those by-products as well as enable access to them in regions where availability is low. Moreover, their joint application can potentially reduce environmental pollution, enlarge the manure benefits to more areas, and enhance the SF efficiency. However, such a strategy might result in increased ammonia emissions when applied to crop residues. Two experiments were implemented to assess ammonia emissions from stubble-covered soil fertilized with manure amended with SF. In Experiment 1 (E1), urea (U) and calcium ammonium nitrate (AN) were applied combined with dairy manure (MAN). In Experiment 2 (E2), urea was combined with acidified pig slurry (APS) and applied just after sowing (T0) or eight days later (T8). The combinations U + MAN and AN + MAN increased the ammonia emissions, while APS decreased the emissions from U, in APS + U combination, by more than 75%. Therefore, manure combined with SF applied on stubble-covered soil should not be recommended. T8 reduced ammonia emissions from U. APS enhanced the efficiency of U, being then an interesting strategy to mitigate ammonia emissions when applied on stubble-covered soil, as in no-tillageinfo:eu-repo/semantics/publishedVersio

Sustained gene expression in the retina by improved episomal vectors

Gene and cellular therapies are nowadays part of therapeutic strategies for the treatment of diverse pathologies. The drawbacks associated with gene therapy-low levels of transgene expression, vector loss during mitosis, and gene silencing-need to be addressed. The pEPI-1 and pEPito family of vectors was developed to overcome these limitations. It contains a scaffold/matrix attachment region, which anchors its replication to cell division in eukaryotic cells while in an extrachromosomal state and is less prone to silencing, due to a lower number of CpG motifs. Recent success showed that ocular gene therapy is an important tool for the treatment of several diseases, pending the overcome of the aforementioned limitations. To achieve sustained gene delivery in the retina, we evaluated several vectors based on pEPito and pEPI-1 for their ability to sustain transgene expression in retinal cells. These vectors stably transfected and replicated in retinal pigment epithelial (RPE) cells. Expression levels were promoter dependent with constitutive promoters cytomegalovirus immediate early promoter (CMV) and human CMV enhancer/human elongation factor 1 alpha promoter yielding the highest levels of transgene expression compared with the retina-specific RPE65 promoter. When injected in C57Bl6 mice, transgene expression was sustained for at least 32 days. Furthermore, the retina-specific RPE65 promoter showed higher efficiency in vivo compared to in vitro. In this study, we demonstrate that by combining tissue-specific promoters with a mitotic stable system, less susceptible to epigenetic silencing such as pEPito-based plasmids, we can achieve prolonged gene expression and a sustained therapeutic effect.Fundacao para a Ciencia e Tecnologia, Portugal [PEst/OE/EQB-LA 0023/2013, SFRH/BD/76873/2011, SFRH/BD/70318/2010, PTDC/SAU/BEB/098475/2008]; European Union [PIRG-GA-2009-249314

Pareto's Law of Income Distribution: Evidence for Germany, the United Kingdom, and the United States

We analyze three sets of income data: the US Panel Study of Income Dynamics PSID), the British Household Panel Survey (BHPS), and the German Socio-Economic Panel (GSOEP). It is shown that the empirical income distribution is consistent with a two-parameter lognormal function for the low-middle income group (97%-99% of the population), and with a Pareto or power law function for the high income group (1%-3% of the population). This mixture of two qualitatively different analytical distributions seems stable over the years covered by our data sets, although their parameters significantly change in time. It is also found that the probability density of income growth rates almost has the form of an exponential function.Comment: Latex2e v1.6; 16 pages with 5 figure

Nonlinear analysis of plane frames using a co-rotating Timoshenko beam element

En este trabajo se describe la formulación corrotacional de un elemento de viga unificado que integra las teorías de vigas de Euler-Bernoulli y de Timoshenko y que no presenta bloqueo por deformación a cortante. La cinemática corrotacional se basa en la separación del movimiento de un sólido en una parte deformacional, y la otra, en movimiento de cuerpo rígido. La parte deformacional del movimiento es representada por tres modos de deformaciones naturales que son generados por el esfuerzo axil, la flexíon pura y la flexíon simple, respectivamente. Los esfuerzos generados por los modos naturales de deformación son auto-equilibrados lo que posibilita la obtención de la matriz de rigidez tangente corrotacional consistente. Se describe de forma detallada la obtención de las matrices de rigideces elástica, geométrica y corrotacional. A través de algunos ejemplos numéricos se muestra la habilidad del elemento de tratar con grandes rotaciones de cuerpo rígido.The present work describes a co-rotating shear flexible beam element without shear locking and integrating Euler-Bernoullis and Timoshenkos beam theories. The co-rotational kinematics is based on the separation of the motion in deformational and rigid body components. The deformation of the beam element is composed by three natural modes of deformation: the extension mode, the symmetric bending mode, and the anti-symmetric bending mode. The respective generalized stresses from these natural modes are self-balanced allowing the achievement of a consistent tangent stiffness matrix. In this paper, it is detailed and deduced all the algebraic steps for the deduction of the elastic stiffness matrix, the geometric stiffness matrix, and the co-rotation stiffness matrix. Some examples are presented and the numerical results demonstrate that the beam element here presented is able to handle large rotations.Peer Reviewe

Online delivery and assessment during COVID-19: safeguarding academic integrity

Globally, the number of COVID-19 cases continues to rise daily despite strict measures being adopted by many countries. Consequently, universities closed down to minimise the face-to-face contacts, and the majority of the universities are now conducting degree programmes through online delivery. Remote online delivery and assessment are novel experiences for many universities, which presents many challenges, particularly when safeguarding academic integrity. For example, invigilated assessments, often considered as more secure, are not an option given the current situation and detecting any cheating would be significantly challenging. This paper reviews assessment security in the digital domain and critically evaluates the practices from different universities in safeguarding academic integrity, including associated challenges

Glyphosate translocation in herbicide tolerant plants

The objective of this study was to evaluate glyphosate translocation in glyphosate-tolerant weed species (I. nil, T. procumbens and S. latifolia) compared to glyphosate-susceptible species (B. pilosa). The evaluations of 14C-glyphosate absorption and translocation were performed at 6, 12, 36 and 72 hours after treatment (HAT) in I. nil and B. pilosa, and only at 72 HAT in the species T. procumbens and S. latifolia. The plants were collected and fractionated into application leaf, other leaves, stems, and roots. In S. latifolia, approximately 88% of the glyphosate remained in the application leaf and a small amount was translocated to roots at 72 HAT. However, 75% of the herbicide applied on T. procumbens remained in the leaf that had received the treatment, with greater glyphosate translocation to the floral bud. It was concluded that the smaller amount of glyphosate observed in S. latifolia and T. procumbens may partly account for their higher tolerance to glyphosate. However, I. nil tolerance to glyphosate may be associated with other factors such as metabolization, root exudation or compartmentalization, because a large amount of the herbicide reached the roots of this species.Objetivou-se neste estudo avaliar a translocação de glyphosate em plantas tolerantes (Ipomoea nil, Tridax procumbens e Spermacoce latifolia) e suscetível (Bidens pilosa) a esse herbicida. As avaliações de absorção e translocação do 14C-glyphosate em I. nil e B. pilosa foram efetuadas às 6, 12, 36 e 72 horas após a aplicação do herbicida (HAT), e em T. procumbens e S. latifolia, às 72 HAT. As plantas foram coletadas e fracionadas em: folha de aplicação, demais folhas, caules e raízes; em T. procumbens e S. latifolia, avaliou-se a presença do produto na inflorescência da planta. Em S. latifolia, aproximadamente 88% do glyphosate permaneceu na folha de aplicação, e pequena quantidade translocou para as raízes. Todavia, em T. procumbens, 75% do herbicida permaneceu na folha que recebeu a aplicação, observando-se maior translocação na inflorescência em relação às raízes. Conclui-se que a menor translocação do glyphosate observada em S. latifolia e T. procumbens pode ser um dos fatores responsáveis pela maior tolerância dessas espécies ao herbicida, ao passo que em I. nil a metabolização, a exsudação radicular ou a compartimentalização podem favorecer a tolerância, já que grande quantidade do produto atingiu as raízes da espécie

Estimating Self-Sustainability in Peer-to-Peer Swarming Systems

Peer-to-peer swarming is one of the \emph{de facto} solutions for distributed content dissemination in today's Internet. By leveraging resources provided by clients, swarming systems reduce the load on and costs to publishers. However, there is a limit to how much cost savings can be gained from swarming; for example, for unpopular content peers will always depend on the publisher in order to complete their downloads. In this paper, we investigate this dependence. For this purpose, we propose a new metric, namely \emph{swarm self-sustainability}. A swarm is referred to as self-sustaining if all its blocks are collectively held by peers; the self-sustainability of a swarm is the fraction of time in which the swarm is self-sustaining. We pose the following question: how does the self-sustainability of a swarm vary as a function of content popularity, the service capacity of the users, and the size of the file? We present a model to answer the posed question. We then propose efficient solution methods to compute self-sustainability. The accuracy of our estimates is validated against simulation. Finally, we also provide closed-form expressions for the fraction of time that a given number of blocks is collectively held by peers.Comment: 27 pages, 5 figure

An alternative order parameter for the 4-state Potts model

We have investigated the dynamic critical behavior of the two-dimensional 4-state Potts model using an alternative order parameter first used by Vanderzande [J. Phys. A: Math. Gen. \textbf{20}, L549 (1987)] in the study of the Z(5) model. We have estimated the global persistence exponent $\theta_g$ by following the time evolution of the probability $P(t)$ that the considered order parameter does not change its sign up to time $t$. We have also obtained the critical exponents $\theta$, $z$, $\nu$, and $\beta$ using this alternative definition of the order parameter and our results are in complete agreement with available values found in literature.Comment: 6 pages, 6 figure