Early suppression of lymphoproliferative response in dogs with natural infection by Leishmania infantum.

Dogs are the domestic reservoirs of zoonotic visceral leishmaniasis caused by Leishmania infantum. Early detection of canine infections evolving to clinically patent disease may be important to leishmaniasis control. In this study we firstly investigated the peripheral blood mononuclear cell (PBMC) response to leishmanial antigens and to polyclonal activators concanavalin A, phytohemagglutinin and pokeweed mitogen, of mixed-breed dogs with natural L. infantum infection, either in presymptomatic or in patent disease condition, compared to healthy animals. Leishmania antigens did not induce a clear proliferative response in any of the animals examined. Furthermore, mitogen-induced lymphocyte proliferation was found strongly reduced not only in symptomatic, but also in presymptomatic dogs suggesting that the cell-mediated immunity is suppressed in progressive canine leishmaniasis. To test this finding, naive Beagle dogs were exposed to natural L. infantum infection in a highly endemic area of southern Italy. Two to 10 months after exposure all dogs were found to be infected by Leishmania, and on month 2 of exposure they all showed a significant reduction in PBMC activation by mitogens. Our results indicate that suppression of the lymphoproliferative response is a common occurrence in dogs already at the beginning of an established leishmanial infection. # 1999 Elsevier Science B.V. All rights reserved

Mechanistic species distribution modeling reveals a niche shift during invasion

Niche shifts of nonnative plants can occur when they colonize novel climatic conditions. However, the mechanistic basis for niche shifts during invasion is poorly understood and has rarely been captured within species distribution models. We quantified the consequence of between-population variation in phenology for invasion of common ragweed (Ambrosia artemisiifolia L.) across Europe. Ragweed is of serious concern because of its harmful effects as a crop weed and because of its impact on public health as a major aeroallergen. We developed a forward mechanistic species distribution model based on responses of ragweed development rates to temperature and photoperiod. The model was parameterized and validated from the literature and by reanalyzing data from a reciprocal common garden experiment in which native and invasive populations were grown within and beyond the current invaded range. It could therefore accommodate between-population variation in the physiological requirements for flowering, and predict the potentially invaded ranges of individual populations. Northern-origin populations that were established outside the generally accepted climate envelope of the species had lower thermal requirements for bud development, suggesting local adaptation of phenology had occurred during the invasion. The model predicts that this will extend the potentially invaded range northward and increase the average suitability across Europe by 90% in the current climate and 20% in the future climate. Therefore, trait variation observed at the population scale can trigger a climatic niche shift at the biogeographic scale. For ragweed, earlier flowering phenology in established northern populations could allow the species to spread beyond its current invasive range, substantially increasing its risk to agriculture and public health. Mechanistic species distribution models offer the possibility to represent niche shifts by varying the traits and niche responses of individual populations. Ignoring such effects could substantially underestimate the extent and impact of invasions

Transport properties of isospin effective mass splitting

We investigate in detail the momentum dependence ($MD$) of the effective in medium Nucleon-Nucleon ($NN$) interaction in the isovector channel. We focus the discussion on transport properties of the expected neutron-proton ($n/p$) effective mass splitting at high isospin density. We look at observable effects from collective flows in Heavy Ion Collisions ($HIC$) of charge asymmetric nuclei at intermediate energies. Using microscopic kinetic equation simulations nucleon transverse and elliptic collective flows in $Au+Au$ collisions are evaluated. In spite of the reduced charge asymmetry of the interacting system interesting $isospin-MD$ effects are revealed. Good observables, particularly sensitive to the $n/p$-mass splitting, appear to be the differences between neutron and proton flows. The importance of more exclusive measurements, with a selection of different bins of the transverse momenta ($p_t$) of the emitted particles, is stressed. In more inclusive data a compensation can be expected from different $p_t$-contributions, due to the microscopic $isospin-MD$ structure of the nuclear mean field in asymmetric matter.Comment: 18 pages, 11 figure

Isospin Dynamics in Heavy Ion Collisions: EoS-sensitive Observables

Heavy Ion Collisions (HIC) represent a unique tool to probe the in-medium nuclear interaction in regions away from saturation and at high nucleon momenta. In this report we present a selection of reaction observables particularly sensitive to the isovector part of the interaction, i.e. to the symmetry term of the nuclear Equation of State (EoS) At low energies the behavior of the symmetry energy around saturation influences dissipation and fragment production mechanisms. Predictions are shown for deep-inelastic and fragmentation collisions induced by neutron rich projectiles. Differential flow measurements will also shed lights on the controversial neutron/proton effective mass splitting in asymmetric matter. The high density symmetry term can be derived from isospin effects on heavy ion reactions at relativistic energies (few AGeV range), that can even allow a ``direct'' study of the covariant structure of the isovector interaction in the hadron medium. Rather sensitive observables are proposed from collective flows and from pion/kaon production. The possibility of the transition to a mixed hadron-quark phase, at high baryon and isospin density, is finally suggested. Some signatures could come from an expected ``neutron trapping'' effect.Comment: 10 pages, 5 figures; espcrc1 style; IX Int.Conf. on Nucleus-Nucleus Collisions, Rio de Janeiro Aug.2006; to appear in Nucl.Phys.

Asymmetric nuclear matter:the role of the isovector scalar channel

We try to single out some qualitative new effects of the coupling to the $\delta$-isovector-scalar meson introduced in a minimal way in a phenomenological hadronic field theory. Results for the equation of state ($EOS$) and the phase diagram of asymmetric nuclear matter ($ANM$) are discussed. We stress the consistency of the $\delta$-coupling introduction in a relativistic approach. New contributions to the slope and curvature of the symmetry energy and the neutron-proton effective mass splitting appear particularly interesting. A more repulsive $EOS$ for neutron matter at high baryon densities is expected. Effects on new critical properties of warm $ANM$, mixing of mechanical and chemical instabilities and isospin distillation, are also presented. The $\delta$ influence is mostly on the {\it isovectorlike} collective response. The results are largely analytical and this makes the physical meaning quite transparent. Implications for nuclear structure properties of drip-line nuclei and for reaction dynamics with Radioactive Beams are finally pointed out.Comment: 12 pages, 10 Postscript figure

Asymmetric nuclear matter in a Hartree-Fock approach to non-linear QHD

The Equation of State (EOS) for asymmetric nuclear matter is discussed starting from a phenomenological hadronic field theory of Serot-Walecka type including exchange terms. In a model with self interactions of the scalar sigma-meson we show that the Fock terms naturally lead to isospin effects in the nuclear EOS. These effects are quite large and dominate over the contribution due to isovector mesons. We obtain a potential symmetry term of "stiff" type, i.e. increasing with baryon density and an interesting behaviour of neutron/proton effective masses of relevance for transport properties of asymmetric dense matter.Comment: 12 pages (LATEX), 3 Postscript figures, revised versio

On the Lorentz structure of the symmetry energy

We investigate in detail the density dependence of the symmetry energy in a relativistic description by decomposing the iso-vector mean field into contributions with different Lorentz covariant properties. We find important effects of the iso-vector, scalar channel (i.e. $\delta$-meson like) on the high density behavior of the symmetry energy. Applications to static properties of finite nuclei and to dynamic situations of heavy ion collisions are explored and related to each other. The nuclear structure studies show only moderate effects originating from the virtual $\delta$ meson. At variance, in heavy ion collisions one finds important contributions on the reaction dynamics arising from the different Lorentz structure of the high density symmetry energy when a scalar iso-vector $\delta$ field is introduced. Particularly interesting is the related neutron/proton effective mass splitting for nucleon transport effects and for resonance and particle production around the threshold. We show that the $\delta$-like channel turns out to be essential for the production of pions, when comparing with experimental data, in particular for high momentum selections.Comment: 30 pages, 12 figures (.eps

Neutron-Proton Differential Flow as a Probe of Isospin-Dependence of Nuclear Equation of State

The neutron-proton differential flow is shown to be a very useful probe of the isospin-dependence of the nuclear equation of state (EOS). This novel approach utilizes constructively both the isospin fractionation and the nuclear collective flow as well as their sensitivities to the isospin-dependence of the nuclear EOS. It also avoids effectively uncertainties associated with other dynamical ingredients of heavy-ion reactions at intermediate energies.Comment: 10 pages + 3 figures. Phys. Rev. Lett. (2000) in pres

Evaluation of rK39 rapid diagnostic tests for canine visceral leishmaniasis : longitudinal study and meta-analysis

Canine visceral leishmaniasis is a vector-borne disease caused by the intracellular parasite Leishmania infantum. It is an important veterinary disease, and dogs are also the main animal reservoir for human infection. The disease is widespread in the Mediterranean area, and parts of Asia and South and Central America, and is potentially fatal in both dogs and humans unless treated. Diagnosis of canine infections requires serological or molecular tests. Detection of infection in dogs is important prior to treatment, and in epidemiological studies and control programmes, and a sensitive and specific rapid diagnostic test would be very useful. Rapid diagnostic tests (RDTs) have been developed, but their diagnostic performance has been reported to be variable. We evaluated the sensitivity of a RDT based on serological detection of the rK39 antigen in a cohort of naturally infected Brazilian dogs. The sensitivity of the test to detect infection was relatively low, but increased with time since infection and the severity of infection. We then carried out a meta-analysis of published studies of rK39 RDTs, evaluating the sensitivity to detect disease and infection. The results suggest that rK39 RDTs may be useful in a veterinary clinical setting, but the sensitivity to detect infection is too low for operational control programmes