Canalopatías en la epilepsia idiopática monogénica en perros

Epilepsy is a common neurological disorder both dogs and humans. Nevertheless, unlike occurs in humans, in dogs it is a badly characterized disease. In humans, it is known from some time ago, that some idiopathic epilepsies present patterns of mendelian inheritance, i.e., caused by the mutation in single genes. The majority of these mutations are in genes codifying by ion channels, being consider as channelopathies. In dogs, the predisposition of some breeds to suffer the disease has aimed to some research groups to study the hereditary basis of idiopathic epilepsy. This review summarizes some aspects relating to the interest to tackle the study of channelopathies in monogenic idiopathic epilepsy in dogs and shows its potential as future diagnostic or therapeutic targets.La epilepsia es una alteración neurológica común tanto en perros como en personas. Sin embargo, al contrario de lo que ocurre en estos últimos, en los perros es una enfermedad mal caracterizada. En personas, se conoce desde hace tiempo que algunas epilepsias idiopáticas, presentan patrones de herencia mendeliana, es decir, causadas por la mutación en un único gen. La mayoría de estas mutaciones ocurren en genes que codifican para canales iónicos, por lo que son consideradas como canalopatías. En perros, la predisposición de algunas razas a padecer la enfermedad ha animado a algunos grupos de investigación a estudiar los patrones de herencia de la epilepsia idiopática. Esta revisión resume algunos aspectos relativos al interés de abordar el estudio de la implicación de canalopatías en la epilepsia idiomática monogénica en perros y presenta su potencial como futuras dianas tanto de diagnóstico como terapéuticas

Highly Efficient TADF OLEDs: How the Emitter–Host Interaction Controls Both the Excited State Species and Electrical Properties of the Devices to Achieve Near 100% Triplet Harvesting and High Efficiency

New emitters that can harvest both singlet and triplet excited states to give 100% internal conversion of charge into light, are required to replace Ir based phosphors in organic light emitting diodes (OLEDs). Molecules that have a charge transfer (CT) excited state can potentially achieve this through the mechanism of thermally activated delayed fluorescence (TADF). Here, it is shown that a D–A charge transfer molecule in the solid state, can emit not only via an intramolecular charge transfer (ICT) excited state, but also from exciplex states, formed between the molecule and the host material. OLEDs based on a previously studied D–A–D molecule in a host TAPC achieves >14% external electroluminescence yield and shows nearly 100% efficient triplet harvesting. In these devices, it is unambiguously established that the triplet states are harvested via TADF, but more interestingly, these results are found to be independent of whether the emitter is the ICT state or the D–A–D/host exciplex

Trigeminal Neuralgia: Literature Review

The trigeminal nerve, fifth equal of cranial nerves, a mixed nerve is considered by possessing motor and sensitive components. The sensitive portion takes to the Nervous System Central somesthesics information from the skin and mucous membrane of great area of the face, being responsible also for a neural disease, known as the Trigeminal Neuralgia. The aim of this study was to review the literature on the main characteristics of Trigeminal Neuralgia, the relevant aspects for the diagnosis and treatment options for this pathology. This neuralgia is characterized by hard pains and sudden, similar to electric discharges, with duration between a few seconds to two minutes, in the trigeminal nerve sensorial distribution. The pain is unchained by light touches in specific points in the skin of the face or for movements of the facial muscles, it can be caused by traumatic sequels or physiologic processes degenerative associate the vascular compression. Prevails in the senior population, frequently in the woman. In a unilateral way it attacks more the maxillary and mandibular divisions, rarely happens in a simultaneous way in the three branches of trigeminal nerve three branches.30115Adams, R.D., Victor, M., (1985) Principles of Neurology, , New York: Mc Graw-Hill Book CompanyBayer, D.B., Stenger, T.G., Trigeminal Neuralgia: An overview (1979) Oral Surgery, 48 (5), pp. 393-399. , http://dx.doi.org/10.1016/0030-4220(79)90064-1Bennetto, L., Patel, N.K., Fuller, G., (2007) Trigeminal Neuralgia and Its Management, 334, pp. 201-205. , http://dx.doi.org/10.1136/bmj.39085.614792.BE, BMJ, PMid:17255614 PMCid:1782012Brow, J.A., Percutaneous balloon compression for trigeminal Neuralgia (2009) Clinical Neurosurgery, 56, pp. 73-76. , PMid:20214036Eskandar, E., Barker, F.G., Rabinov, J.D., Case records of the Massachusetts General Hospital. Case21-2006. A61-year-old man with left-sided facial pain (2006) New England Journal of Medicine, 355, pp. 183-188. , http://dx.doi.org/10.1056/NEJMcpc069011, PMid:16837683Fardy, M.J., Patton, D.W., Complication associated with peripheral alcohol injections in the management of trigeminal neuralgia (1994) British Journal of Oral and Maxillofacial Surgery, 32 (6), pp. 387-391. , http://dx.doi.org/10.1016/0266-4356(94)90031-0Frizzo, H.M., Hasse, P.N., Veronese, R.M., Neuralgia do Trigêmeo: Revisão Bibliográfica Analítica (2004) Revista De Cirurgia E Traumatologia Buco-Maxilo-Facial, 4 (4), pp. 212-217Galassi, C., Blasi, G., Galassi, G., Serra, M., Faverio, A., Nevralgie trigeminali ed altre algie del capo (1985) Parodontologia E Stomatologia (nuova), 2, pp. 45-160Goto, F., Ishizaki, K., Yoshikawa, D., Obata, H., Arii, H., Terada, M., The long lasting effects of peripheral nerve blocks for trigeminal neuralgia using a high concentration of tetracaine dissolved in bupivacaine (1999) Pain, 79, pp. 101-103. , http://dx.doi.org/10.1016/S0304-3959(98)00156-0Gusmão, S., Magaldi, M., Arantes, A., Rizotomia trigeminal por radiofrequência para tratamento da neuralgia do trigêmeo: Resultados e modificação técnica (2003) Arquivos De Neuro-Psiquiatria, 61 (2), pp. 434-440. , http://dx.doi.org/10.1590/S0004-282X2003000300020, PMid:12894280Hotta, T.H., Bataglion, A., Bataglion, C., Bezzon, O.L., Involvemente of dental occlusion and trigeminal neuralgia: A clinical report (1997) Journal of Prosthetic Dentistry, 77 (4), pp. 343-345. , http://dx.doi.org/10.1016/S0022-3913(97)70155-0Jacob, R.P., Rhoton, J.A.L., Diagnosis and Nonoperative Management of Trigeminal Neuralgia (1996) In: YOUMANS, JR. Neurological Surgery, 5, pp. 3376-3385. , 4th Ed. W. B. Saunders CompanyJanneta, P.G., Gross (Mesoscopic) description of the human trigeminal nerve and ganglion (1963) Journal of Neurosurgery, 20, pp. 109-111Katusic, S., Beard, M.B., Bergstralh, E., Kurland, L.T., Incidence and clinical features of trigeminal neuralgia, Rochester, Minnesota:1945-1984 (1990) Annals of Neurology, 27, pp. 89-95. , http://dx.doi.org/10.1002/ana.410270114, PMid:2301931Krafft, R.M., Trigeminal neuralgia (2008) American Family Physician, 77, pp. 1291-1296. , PMid:18540495Linskey, M.E., Jho, H.D., Jannetta, P.J., Microvascular decompression for trigeminal neuralgia caused by vertebrobasilar compression (1994) Journal of Neurosurgery, 81, pp. 1-9. , http://dx.doi.org/10.3171/jns.1994.81.1.0001, PMid:8207508Loh, H.S., Ling, S.Y., Shanmuhasuntharam, P., Zain, R., Yeo, J.F., Khoo, S.P., Trigeminal neuralgia. A retrospective survey of a sample of patients in Singapore and Malaysia (1998) Australian Dental Journal, 43 (3), pp. 188-191. , PMid:9707784Luna, E.B., Graça, L.F.A., Silva, D.C.O., Bérzin, F., Silva, Z., Souza, G.C., Mitri, F.F., Aspectos Anatômicos e Patológicos da Neuralgia do Trigêmeo: Uma revisão da literatura para estudantes e profissionais da saúde (2010) Bioscience Journal, 26 (4), pp. 661-674Maestri, J.M., Holzer, F., Fisiopatología de la neuralgia del trigémeno (1993) Revista Chilena De Neuro-Psiquiatria, 31 (3), pp. 317-321Mattos, J.M.B., Bueno, F.V., Mattos, L.R., Neuralgia do Trigêmeo: Um novo protocolo de tratamento clínico (2005) Revista Dor, 6 (4), pp. 652-656Meneses, M.S., Clemente, R., Russ, H.H., Traitement microchirurgical de la névralgie du trijumeau (1995) Neurochirurgie, 41 (5), pp. 349-352. , PMid:8577355Monzillo, P.H., Sanvito, W.L., Costa, A.R., Cluster-Tic, S., Syndrome: Report of five new cases (2000) Arquivos De Neuro-Psiquiatria, 58, pp. 518-521. , http://dx.doi.org/10.1590/S0004-282X2000000300019, PMid:10920416Neville, B.W., Damm, D.D., Allen, C.M., Bouquot, J.E., (2004) Patologia Oral E Maxilofacial.2.ed, pp. 865-867. , Rio de Janeiro: Guanabara KooganNurmikko, T.J., Eldridge, P.R., (2001) Trigeminal Neuralgia: Pathophysiology, Diagnosis and Current Treatment. BJA: British Journal of Anaesthesia, 87, pp. 117-132. , http://dx.doi.org/10.1093/bja/87.1.117Patterson, C.W., Trigeminal neuralgia-a dental diagnosis challenge (1999) Northwest Dentistry, 78 (3), pp. 19-24Peters, G., Nurmikko, T.J., Peripheral and gasserian ganglion-level precedures for the treatment of trigeminal neuralgia (2002) Clinical Journal of Pain, 18 (1), pp. 28-34. , http://dx.doi.org/10.1097/00002508-200201000-00005, PMid:11803300Quesada, G.A.T., Baptista, C.E., Daiana, S.P., Douglas, L.F., Neuralgia Trigeminal-do diagnóstico ao tratamento (2005) Revista Dentística, (11)Siqueira, S.R.D.T., Nóbrega, J.C.M., Valle, L.B.S., Teixeira, M.J., Siqueira, J.T.T., Clinical aspects and dental procedures (2004) Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, 98 (3), pp. 311-315Toda, K., Trigeminal neuralgia: Symptoms, diagnosis, classification, and related disorders (2007) Oral Science International, 4 (1), pp. 1-9. , http://dx.doi.org/10.1016/S1348-8643(07)80006-1Tronnier, V.M., Rasche, D., Hamer, J., Treatment of idiopathic trigeminal neuralgia: Comparison of long-term outcome after radiofrequency rhizotomy and microvascular decompression (2001) Neurosurgery, 48, pp. 1261-1268. , PMid:11383728Türp, J.C., Gobetti, J.P., Trigeminal neuralgia versus atypical facial pain: A review of the literature and case report (1996) Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, 81 (4), pp. 424-432Yoon, K.B., Wiles, J.R., Miles, J.B., Nurmikko, T.J., Long-term outcome of percutaneous thermocoagulation for trigeminal neuralgia (1999) Anaesthesia, 54 (80), pp. 798-808Yoshimasu, F., Kurland, L.T., Elvelvack, L.R., Tic dolourex in Rochester, Minnesota (1972) Neurology, 22, pp. 952-956. , http://dx.doi.org/10.1212/WNL.22.9.952, 1945-1969. PMid:467338

METODOLOGIAS DE DESNUDAMENTO PARCIAL DE OÓCITOS BOVINOS MATURADOS E SUBMETIDOS À VITRIFICAÇÃO

Different methods of partial denudation of maturated bovine oocytes were evaluated for in vitro embryo production and vitrification procedures, in three experiments. First the effect of partial denudation by successive pipetting was evaluated. In the second experiment, the denudation by pipetting was compared with the use of hyaluronidase, and finally, the effect of both methods was evaluated in regard to the vitrification of oocytes. Oocytes were submitted to treatments after 22 hours of maturation in TCM 199 medium. For the vitrification, they were firstly exposed for 30 seconds to an equilibrium solution and for 20 seconds to a vitrification solution (20% EG + 20% DMSO + 0.3M SUC), loaded in open pulled straws (OPS) and plunged into liquid nitrogen. The rewarming of the oocytes suspensions was carried out by plunged them in decreasing sucrose concentrations. Oocytes of all treatments were submitted to an additional 2 hours maturation period, followed by fertilization. Presumptive zygotes were cultured in SOFaaci medium. In the first experiment the denudation resulted in a decreased blastocyst rate (28.7% to 20.5% PDiferentes metodologias de desnudamento parcial de oócitos bovinos maturados foram avaliadas na produção in vitro de embriões e na vitrificação, em três experimentos. No primeiro avaliou-se o efeito do desnudamento parcial realizado por sucessivas pipetagens. No segundo, comparou-se o desnudamento através de sucessivas pipetagens, com o realizado pela enzima hialuronidase e no terceiro, avaliou-se a influência das duas metodologias na vitrificação de oócitos. Os oócitos foram submetidos aos tratamentos após 22 horas de maturação em meio TCM 199. Para a vitrificação, foram expostos por 30 segundos a uma solução de equilíbrio e 20 segundos a uma solução de vitrificação (20%EG + 20% Dimitilsufoxido (DMSO) + 0,3MSAC), envasados em palhetas abertas e estiradas (OPS) e mergulhados em N2 líquido. O reaquecimento foi realizado com exposição a concentrações decrescentes de sacarose. Os oócitos de todos os tratamentos foram submetidos a duas horas adicionais de maturação, seguida da fecundação e os possíveis zigotos cultivados em meio SOFaaci. No experimento I foi observado um decréscimo de 28,7% para 20,5% na taxa de blastocistos com o emprego do desnudamento (

Waist circumference as a vital sign in clinical practice: a Consensus Statement from the IAS and ICCR Working Group on Visceral Obesity

Despite decades of unequivocal evidence that waist circumference provides both independent and additive information to BMI for predicting morbidity and risk of death, this measurement is not routinely obtained in clinical practice. This Consensus Statement proposes that measurements of waist circumference afford practitioners with an important opportunity to improve the management and health of patients. We argue that BMI alone is not sufficient to properly assess or manage the cardiometabolic risk associated with increased adiposity in adults and provide a thorough review of the evidence that will empower health practitioners and professional societies to routinely include waist circumference in the evaluation and management of patients with overweight or obesity. We recommend that decreases in waist circumference are a critically important treatment target for reducing adverse health risks for both men and women. Moreover, we describe evidence that clinically relevant reductions in waist circumference can be achieved by routine, moderate-intensity exercise and/or dietary interventions. We identify gaps in the knowledge, including the refinement of waist circumference threshold values for a given BMI category, to optimize obesity risk stratification across age, sex and ethnicity. We recommend that health professionals are trained to properly perform this simple measurement and consider it as an important 'vital sign' in clinical practice

Kinin b(1) receptor in adipocytes regulates glucose tolerance and predisposition to obesity

BACKGROUND: Kinins participate in the pathophysiology of obesity and type 2 diabetes by mechanisms which are not fully understood. Kinin B(1) receptor knockout mice (B(1) (-/-)) are leaner and exhibit improved insulin sensitivity. METHODOLOGY/PRINCIPAL FINDINGS: Here we show that kinin B(1) receptors in adipocytes play a role in controlling whole body insulin action and glucose homeostasis. Adipocytes isolated from mouse white adipose tissue (WAT) constitutively express kinin B(1) receptors. In these cells, treatment with the B(1) receptor agonist des-Arg(9)-bradykinin improved insulin signaling, GLUT4 translocation, and glucose uptake. Adipocytes from B(1) (-/-) mice showed reduced GLUT4 expression and impaired glucose uptake at both basal and insulin-stimulated states. To investigate the consequences of these phenomena to whole body metabolism, we generated mice where the expression of the kinin B(1) receptor was limited to cells of the adipose tissue (aP2-B(1)/B(1) (-/-)). Similarly to B(1) (-/-) mice, aP2-B(1)/B(1) (-/-) mice were leaner than wild type controls. However, exclusive expression of the kinin B(1) receptor in adipose tissue completely rescued the improved systemic insulin sensitivity phenotype of B(1) (-/-) mice. Adipose tissue gene expression analysis also revealed that genes involved in insulin signaling were significantly affected by the presence of the kinin B(1) receptor in adipose tissue. In agreement, GLUT4 expression and glucose uptake were increased in fat tissue of aP2-B(1)/B(1) (-/-) when compared to B(1) (-/-) mice. When subjected to high fat diet, aP2-B(1)/B(1) (-/-) mice gained more weight than B(1) (-/-) littermates, becoming as obese as the wild types. CONCLUSIONS/SIGNIFICANCE: Thus, kinin B(1) receptor participates in the modulation of insulin action in adipocytes, contributing to systemic insulin sensitivity and predisposition to obesity

Transfer learning for galaxy morphology from one survey to another

© 2018 The Author(s). Published by Oxford University Press on behalf of the Royal Astronomical Society.Deep Learning (DL) algorithms for morphological classification of galaxies have proven very successful, mimicking (or even improving) visual classifications. However, these algorithms rely on large training samples of labelled galaxies (typically thousands of them). A key question for using DL classifications in future Big Data surveys is how much of the knowledge acquired from an existing survey can be exported to a new dataset, i.e. if the features learned by the machines are meaningful for different data. We test the performance of DL models, trained with Sloan Digital Sky Survey (SDSS) data, on Dark Energy survey (DES) using images for a sample of $\sim$5000 galaxies with a similar redshift distribution to SDSS. Applying the models directly to DES data provides a reasonable global accuracy ($\sim$ 90%), but small completeness and purity values. A fast domain adaptation step, consisting in a further training with a small DES sample of galaxies ($\sim$500-300), is enough for obtaining an accuracy > 95% and a significant improvement in the completeness and purity values. This demonstrates that, once trained with a particular dataset, machines can quickly adapt to new instrument characteristics (e.g., PSF, seeing, depth), reducing by almost one order of magnitude the necessary training sample for morphological classification. Redshift evolution effects or significant depth differences are not taken into account in this study.Peer reviewedFinal Accepted Versio