Time-elapsed synchrotron-light microstructural imaging of femoral neck fracture

Time-elapsed micro-computed-tomography (μCT) imaging allows studying bone micromechanics. However, no study has yet performed time-elapsed μCT imaging of human femoral neck fractures. We developed a protocol for time-elapsed synchrotron μCT imaging of the microstructure in the entire proximal femur, while inducing clinically-relevant femoral neck fractures. Three human cadaver femora (females, age: 75-80 years) were used. The specimen-specific force to be applied at each load step was based on the specimens' strength estimated a priori using finite-element analysis of clinical CT images. A radio-transparent compressive stage was designed for loading the specimens while recording the applied load during synchrotron μCT scanning. The total μCT scanning field of view was 146 mm wide and 131 mm high, at 29.81 µm isotropic pixel size. Specimens were first scanned unloaded, then under incremental load steps, each equal to 25% of the estimated specimens' strength, and ultimately after fracture. Fracture occurred after 4-5 time-elapsed load steps, displaying sub-capital fracturing of the femoral neck, in agreement with finite-element predictions. Time-elapsed μCT images, co-registered to those of the intact specimen, displayed the proximal femur microstructure under progressive deformation up to fracture. The images showed (1) a spatially heterogeneous deformation localized in the proximal femoral head; (2) a predominantly elastic recovery, after load removal, of the diaphyseal and trochanteric regions and; (3) post-fracture residual displacements, mainly localized in the fractured region. The time-elapsed μCT imaging protocol developed and the high resolution images generated, made publicly available, may spur further research into human femur micromechanics and fracture.Saulo Martelli, Egon Perill

Role of surgical setting and patients-related factors in predicting the occurrence of postoperative pulmonary complications after abdominal surgery

OBJECTIVE: The aim of this retrospective study was to evaluate the role of surgical setting (urgent vs. elective) and approach (open vs. laparoscopic) in affecting postoperative pulmonary complications (PPCs) prevalence in patients undergoing abdominal surgery. PATIENTS AND METHODS: After local Ethical Committee approval, 409 patients who had undergone abdominal surgery between January and December 2014 were included in the final analysis. PPCs were defined as the development of one of the following new findings: respiratory failure, pulmonary infection, aspiration pneumonia, pleural effusion, pneumothorax, atelectasis on chest X-ray, bronchospasm or un-planned urgent re-intubation. RESULTS: PPCs prevalence was greater in urgent (33%) vs. elective setting (7%) (chi(2) with Yates correction: 44; p=0.0001) and in open (6%) vs. laparoscopic approach (1.9%) (chi(2) with Yates correction: 12; p=0.0006). PPCs occurrence was positively correlated with in-hospital mortality (Biserial Correlation r=0.37; p=0.0001). Logistic regression showed that urgent setting (p=0.000), Ariscat (Assess Respiratory Risk in Surgical Patients in Catalonia) score (p=0.004), and age (p=0.01) were predictors of PPCs. A cutoff of 23 for Ariscat score was also identified as determining factor for PPCs occurrence with 94% sensitivity and 29% specificity. CONCLUSIONS: Patients undergoing abdominal surgery in an urgent setting were exposed to a higher risk of PPCs compared to patients scheduled for elective procedures. Ariscat score fitted with PPCs prevalence and older patients were exposed to a higher risk of PPCs. Prospective studies are needed to confirm these result

Body anthropometry and bone strength conjointly determine the risk of hip fracture in a sideways fall

We hypothesize that variations of body anthropometry, conjointly with the bone strength, determine the risk of hip fracture. To test the hypothesis, we compared, in a simulated sideways fall, the hip impact energy to the energy needed to fracture the femur. Ten femurs from elderly donors were tested using a novel drop-tower protocol for replicating the hip fracture dynamics during a fall on the side. The impact energy was varied for each femur according to the donor’s body weight, height and soft-tissue thickness, by adjusting the drop height and mass. The fracture pattern, force, energy, strain in the superior femoral neck, bone morphology and microarchitecture were evaluated. Fracture patterns were consistent with clinically relevant hip fractures, and the superior neck strains and timings were comparable with the literature. The hip impact energy (11 – 95 J) and the fracture energy (11 – 39 J) ranges overlapped and showed comparable variance (CV = 69 and 61%, respectively). The aBMD-based definition of osteoporosis correctly classified 7 (70%) fracture/non-fracture cases. The incorrectly classified cases presented large impact energy variations, morphology variations and large subcortical voids as seen in microcomputed tomography. In conclusion, the risk of osteoporotic hip fracture in a sideways fall depends on both body anthropometry and bone strength

Evaluating the freeze–thaw phenomenon in sandwich-structured composites via numerical simulations and infrared thermography

The water ingress phenomenon in sandwich-structured composites used in the aerospace/aeronautical sector is a current issue. This type of defect can cause in the course of time several other defects at the boundary, such as corrosions, deformations, detachments. In fact, water may change its state of physical matter going towards the freeze–thaw cycle caused by the atmosphere re-entry of, e.g. space probes. In this work, the alveoli of a composite laminate have been filled with water, which was initially transformed into ice. By taking into account, the known quantity of water, the freeze–thaw cycle was simulated by Comsol Multiphysics® software, reproducing exactly the shape of the sandwich as well as the real conditions in which it was subsequently subjected in a climatic chamber. The experimental part consisted of monitoring the front side of the specimen by means of a thermal camera operating into the long-wave infrared spectrum, and by setting both the temperature and the relative humidity of the test chamber according to the values imposed during the numerical simulation step. It was found that the numerical and experimental temperature trends are in good agreement with each other since the model was built by following a physico-chemical point-of-view. It was also seen that the application of the independent component thermography (ICT) technique was able both to retrieve the positions of the defects (i.e. the water inclusions) and to characterize the defects in which a detachment (fabricated between the fibres and the resin) is present; the latter was realized above an inclusion caused by the water ingress. To the best of our knowledge, this is the first time that ICT is applied to satisfy this purpose.Postprint (author's final draft

Enhanced infrared sparse pattern extraction and usage for impact evaluation of basalt-carbon hybrid composites by pulsed thermography

Nowadays, infrared thermography, as a widely used non-destructive testing method, is increasingly studied for impact evaluation of composite structures. Sparse pattern extraction is attracting increasing attention as an advanced post-processing method. In this paper, an enhanced sparse pattern extraction framework is presented for thermographic sequence processing and defect detection. This framework adapts cropping operator and typical component extraction as a preprocessing step to reduce the dimensions of raw data and applies sparse pattern extraction algorithms to enhance the contrast on the defect area. Different cases are studied involving several defects in four basalt-carbon hybrid fiber-reinforced polymer composite laminates. Finally, comparative analysis with intensity distribution is carried out to verify the effectiveness of contrast enhancement using this framework

Application of in vivo micro-computed tomography in the temporal characterisation of subchondral bone architecture in a rat model of low-dose monosodium iodoacetate-induced osteoarthritis

The electronic version of this article is the complete one and can be found online at: http://arthritis-research.com/content/13/6/R210Introduction: Osteoarthritis (OA) is a complex, multifactorial joint disease affecting both the cartilage and the subchondral bone. Animal models of OA aid in the understanding of the pathogenesis of OA and testing suitable drugs for OA treatment. In this study we characterized the temporal changes in the tibial subchondral bone architecture in a rat model of low-dose monosodium iodoacetate (MIA)-induced OA using in vivo micro-computed tomography (CT). Methods: Male Wistar rats received a single intra-articular injection of low-dose MIA (0.2 mg) in the right knee joint and sterile saline in the left knee joint. The animals were scanned in vivo by micro-CT at two, six, and ten weeks post-injection, analogous to early, intermediate, and advanced stages of OA, to assess architectural changes in the tibial subchondral bone. The articular cartilage changes in the tibiae were assessed macroscopically and histologically at ten weeks post-injection. Results: Interestingly, tibiae of the MIA-injected knees showed significant bone loss at two weeks, followed by increased trabecular thickness and separation at six and ten weeks. The trabecular number was decreased at all time points compared to control tibiae. The tibial subchondral plate thickness of the MIA-injected knee was increased at two and six weeks and the plate porosity was increased at all time points compared to control. At ten weeks, histology revealed loss of proteoglycans, chondrocyte necrosis, chondrocyte clusters, cartilage fibrillation, and delamination in the MIA-injected tibiae, whereas the control tibiae showed no changes. Micro-CT images and histology showed the presence of subchondral bone sclerosis, cysts, and osteophytes. Conclusions: These findings demonstrate that the low-dose MIA rat model closely mimics the pathological features of progressive human OA. The low-dose MIA rat model is therefore suitable to study the effect of therapeutic drugs on cartilage and bone in a non-trauma model of OA. In vivo micro-CT is a non-destructive imaging technique that can track structural changes in the tibial subchondral bone in this animal model, and could also be used to track changes in bone in preclinical drug intervention studies for OA treatments.Geetha Mohan, Egon Perilli, Julia S Kuliwaba, Julia M Humphries, Ian H Parkinson and Nicola L Fazzalar

Damage tolerance and toughness of elderly human femora

Observations of elastic instability of trabecular bone cores supported the analysis of cortical thickness for predicting bone fragility of the hip in people over 60 years of age. Here, we falsified the hypothesis that elastic instability causes minimal energy fracture by analyzing, with a micrometric resolution, the deformation and fracture behavior of entire femora. Femur specimens were obtained from elderly women aged between 66 – 80 years. Microstructural images of the proximal femur were obtained under 3 – 5 progressively increased loading steps and after fracture. Bone displacements, strain, load bearing and energy absorption capacity were analyzed. Elastic instability of the cortex appeared at early loading stages in regions of peak compression. No elastic instability of trabecular bone was observed. The subchondral bone displayed local crushing in compression at early loading steps and progressed to 8 – 16% compression before fracture. The energy absorption capacity was proportional to the displacement. Stiffness decreased to near-zero values before fracture. Three-fourth of the fracture energy (10.2 – 20.2 J) was dissipated in the final 25% force increment. Fracture occurred in regions of peak tension and shear, adjacent to the location of peak compression, appearing immediately before fracture. Minimal permanent deformation was visible along the fracture surface. Elastic instability modulates the interaction between cortical and trabecular bone promoting an elastically stable fracture behavior of the femur organ, load bearing capacity, toughness, and damage tolerance. These findings will advance current methods for predicting hip fragility

A Novel Approach for an Integrated Straw tube-Microstrip Detector

We report on a novel concept of silicon microstrips and straw tubes detector, where integration is accomplished by a straw module with straws not subjected to mechanical tension in a Rohacell $^{\circledR}$ lattice and carbon fiber reinforced plastic shell. Results on mechanical and test beam performances are reported on as well.Comment: Accepted by Transactions on Nuclear Science (2005). 11 pages, 9 figures, uses lnfprep.st

Base of the Toarcian Stage of the Lower Jurassic defined by the Global Boundary Stratotype Section and Point (GSSP) at the Peniche section (Portugal)

This is the final version of the article. Available from the publisher via the DOI in this record.The Global Stratotype Section and Point (GSSP) for the base of Toarcian Stage, Lower Jurassic, is placed at the base of micritic limestone bed 15e at Ponta do Trovão (Peniche, Lusitanian Basin, Portugal; coordinates: 39°22'15''N, 9°23'07''W), 80km north of Lisbon, and coincides with the mass occurrence of the ammonite Dactylioceras (Eodactylites). The Pliensbachian/ Toarcian boundary (PLB/TOA) is contained in a continuous section forming over 450m of carbonate-rich sediments. Tectonics, syn-sedimentary disturbance, metamorphism or significant diagenesis do not significantly affect this area. At the PLB/TOA, no vertical facies changes, stratigraphical gaps or hiatuses have been recorded. The base of the Toarcian Stage is marked in the bed 15e by the first occurrence of D. (E.) simplex, co-occurring with D. (E.) pseudocommune and D. (E.) polymorphum. The ammonite association of D. (Eodactylites) ssp. and other species e.g. Protogrammoceras (Paltarpites) cf. paltum, Lioceratoides aff. ballinense and Tiltoniceras aff. capillatum is particularly significant for the boundary definition and correlation with sections in different basins. Ammonites of the PLB/ TOA are taxa characteristic of both the Mediterranean and Northwest European provinces that allow reliable, global correlations. The PLB/TOA is also characterized by other biostratigraphical markers (brachiopods, calcareous nannofossils, ostracods and benthic foraminifers) and by high-resolution stable carbon and oxygen isotopes, and 87Sr/86Sr ratios that show distinctive changes just above the PLB/TOA, thus providing additional, powerful tools for global correlations. The PBL-TOA lies at the end of a second (and third) order cycle of sea-level change, and the top of bed 15e is interpreted as a sequence boundary. Cyclostratigraphy analysis is available for the Lower Toarcian of Ponta do Trovão. Detailed correlations with the Almonacid de la Cuba section (Iberian Range, Spain) provide complementary data of the ammonite succession in the Northwest European Hawskerense and Paltum Subzones, and magnetostratigraphical data that allow supraregional correlations. The proposal was voted on by the Toarcian Working Group in June, 2012, and by the International Subcommission on Jurassic Stratigraphy in September, 2012, approved by the ICS in November, 2014, and ratified by the IUGS in December, 2014. With this Toarcian GSSP, all international stages of the Lower Jurassic have been officially defined.Several scientists have been members of the Toarcian Working Group. We would like to acknowledge all of them. We are also grateful to the ISJS and ICS members who have made valuable comments on a previous version of this manuscript. We warmly thank Marc Philippe for his help with the literature on Pliensbachian/Toarcian continental successions. We warmly thank Christian Meister and Jim Ogg for their helpful review. Constructive remarks by Jim Ogg on an early version of the paper were greatly appreciated. We also acknowledge the precious help of David Besson for providing the ammonite specimens from the Mouterde collection (Musée des Confluences, Lyon). Ammonite photographs were taken by Emmanuel Robert (Collections de Géologie de Lyon). This paper is dedicated to the memory of Abbé René Mouterde and Serge Elmi, who died in 2007 after having been for years the main supporters of the Peniche section as GSSP of Toarcian Stage. Calcareous nannofossil slides are curated at the Collections de Géologie de Lyon (No. FSL 766535-766617). This work has been supported by the BIOSCALES Project (POCTI/ 36438/PAL/2000), coordinated by the Universidade NOVA de Lisboa; R. B. Rocha thanks the support of A. F. Soares, J. C. Kullberg, P. S. Caetano and P. H. Verdial. Financial support was provided to L. V. Duarte, S. Pinto and M. C. Cabral by Projects PDCTE/CTA/44907/2002 and PTDC/CTE-GIX/098968/2008