SARS-CoV-2 in patients with cancer: possible role of mimicry of human molecules by viral proteins and the resulting anti-cancer immunity

A few reports suggest that molecular mimicry can have a role in determining the more severe and deadly forms of COVID-19, inducing endothelial damage, disseminated intravascular coagulation, and multiorgan failure. Heat shock proteins/molecular chaperones can be involved in these molecular mimicry phenomena. However, tumor cells can display on their surface heat shock proteins/molecular chaperones that are mimicked by SARS-CoV-2 molecules (including the Spike protein), similarly to what happens in other bacterial or viral infections. Since molecular mimicry between SARS-CoV-2 and tumoral proteins can elicit an immune reaction in which antibodies or cytotoxic cells produced against the virus cross-react with the tumor cells, we want to prompt clinical studies to evaluate the impact of SARS-CoV-2 infection on prognosis and follow up of various forms of tumors. These topics, including a brief historical overview, are discussed in this paper

Male phenotypic diversity experienced during ontogeny mediates female mate choice in guppies

This is the author accepted manuscript. The final version is available from Oxford University Press via the DOI in this record.Early social experience can be important in shaping female mate choice. Previous work has shown that females adjust their decisions based on the distribution of male sexual trait values encountered during development. However, other phenotypic features could be important in the formation of mate preferences if, for example, they provide additional information about the males available. Here, we examined how the level of overall phenotypic variance (independent of trait values) experienced during ontogeny, mediated female choice in guppies, Poecilia reticulata. Developing females were reared with males either all different in colouration or all similar in colouration or with adult females representing high variance, low variance and no experience of male variance respectively. We found that females were more sexually responsive when reared with females only than in either of the male treatments. When reared with males, responsiveness was greater in the low-variance compared to the high variance treatment. Moreover, females had stronger sexual preferences following rearing in the high variance compared to the low variance condition. In turn, males switched mating tactics, increasing the rate of coerced copulation attempts when facing choosier females, possibly to balance the loss in mating opportunities. Taken together, these results demonstrate the adaptive plasticity of female mating decisions and the dynamic selection pressures they might impose on the evolution of male sexual traits, potentially contributing to the maintenance of the extreme polymorphism found in male colour patterns.This work was supported by the University of Exeter through an Exeter Graduate Fellow studentship

Shock acceleration as origin of the radio relic in A521?

We present new high sensitivity observations of the radio relic in A521 carried out with the Giant Metrewave Radio Telescope at 327 MHz and with the Very Large Array at 4.9 and 8.5 GHz. We imaged the relic at these frequencies and carried out a detailed spectral analysis, based on the integrated radio spectrum between 235 MHz and 4.9 GHz, and on the spectral index image in the frequency range 327-610 MHz. To this aim we used the new GMRT observations and other proprietary as well as archival data. We also searched for a possible shock front co-located with the relic on a short archival Chandra X-ray observation of the cluster. The integrated spectrum of the relic is consistent with a single power law; the spectral index image shows a clear trend of steepening going from the outer portion of the relic toward the cluster centre. We discuss the origin of the source in the light of the theoretical models for the formation of cluster radio relics. Our results on the spectral properties of the relic are consistent with acceleration of relativistic electrons by a shock in the intracluster medium. This scenario is further supported by our finding of an X-ray surface brightness edge coincident with the outer border of the radio relic. This edge is likely a shock front.Comment: 13 pages, 12 figures, accepted for publication in A&

The Extended GMRT Radio Halo Survey I: New upper limits on radio halos and mini-halos

A fraction of galaxy clusters host diffuse radio sources called radio halos, radio relics and mini-halos. We present the sample and first results from the Extended GMRT Radio Halo Survey (EGRHS)- an extension of the GMRT Radio Halo Survey (GRHS, Venturi et al. 2007, 2008). It is a systematic radio survey of galaxy clusters selected from the REFLEX and eBCS X-ray catalogs . Analysis of GMRT data at 610/ 235/ 325 MHz on 12 galaxy clusters are presented. We report the detection of a newly discovered mini-halo in the cluster RXJ1532.9+3021 at 610 MHz. A small scale relic (~200 kpc) is suspected in the cluster Z348. We do not detect cluster-scale diffuse emission in 11 clusters. Robust upper limits on the detection of radio halo of size of 1 Mpc are determined. We also present upper limits on the detections of mini-halos in a sub-sample of cool-core clusters. The upper limits for radio halos and mini-halos are plotted in the radio power- X-ray luminosity plane and the correlations are discussed. Diffuse extended emission, not related to the target clusters, but detected as by-products in the sensitive images of two of the cluster fields (A689 and RXJ0439.0+0715) are reported. Based on the information about the presence of radio halos (or upper limits), available on 48 clusters out of the total sample of 67 clusters (EGRHS+GRHS), we find that ~23% of the clusters host radio halos. The radio halo fraction rises to ~31%, when only the clusters with X-ray luminosities >8x10^44 erg/s are considered. Mini-halos are found in ~50 % of cool-core clusters. A qualitative examination of the X-ray images of the clusters with no diffuse radio emission indicates that a majority of these clusters do not show extreme dynamical disturbances and supports the idea that mergers play an important role in the generation of radio halos/relics.Comment: 21 pages, 18 figures, 3 tables, accepted for publication in A&

The Extended GMRT Radio Halo Survey II: Further results and analysis of the full sample

The intra-cluster medium contains cosmic rays and magnetic fields that are manifested through the large scale synchrotron sources, termed as radio halos, relics and mini-halos. The Extended Giant Metrewave Radio Telescope (GMRT) Radio Halo Survey (EGRHS) is an extension of the GMRT Radio Halo Survey (GRHS) designed to search for radio halos using GMRT 610/235 MHz observations. The GRHS+EGRHS consists of 64 clusters in the redshift range 0.2 -- 0.4 that have an X-ray luminosity larger than 5x10^44 erg/s in the 0.1 -- 2.4 keV band and with declinations > -31 deg in the REFLEX and eBCS X-ray cluster catalogues. In this second paper in the series, GMRT 610/235 MHz data on the last batch of 11 galaxy clusters and the statistical analysis of the full sample are presented. A new mini-halo in RXJ2129.6+0005 and candidate diffuse sources in Z5247, A2552 and Z1953 are discovered. A unique feature of this survey are the upper limits on the detections of 1 Mpc sized radio halos; 4 new are presented here making a total of 31 in the survey. Of the sample, 58 clusters that have adequately sensitive radio information were used to obtain the most accurate occurrence fractions so far. The occurrence of radio halos in our X-ray selected sample is ~22%, that of mini-halos is 13% and that of relics is ~5%. The radio power - X-ray luminosity diagrams for the radio halos and mini-halos with the detections and upper limits are presented. The morphological estimators namely, centroid shift (w), concentration parameter (c) and power ratios (P_3/P_0) derived from the Chandra X-ray images are used as proxies for the dynamical states of the GRHS+EGRHS clusters. The clusters with radio halos and mini-halos occupy distinct quadrants in the c-w, c-P_3/P_0 and w - P_3/P_0 planes, corresponding to the more and less morphological disturbance, respectively. The non-detections span both the quadrants.Comment: 24 pages, 5 tables, 25 figures, accepted for publication in A&

153 MHz GMRT follow-up of steep-spectrum diffuse emission in galaxy clusters

In this paper we present new high sensitivity 153 MHz Giant Meterwave Radio Telescope follow-up observations of the diffuse steep spectrum cluster radio sources in the galaxy clusters Abell 521, Abell 697, Abell 1682. Abell 521 hosts a relic, and together with Abell 697 it also hosts a giant very steep spectrum radio halo. Abell 1682 is a more complex system with candidate steep spectrum diffuse emission. We imaged the diffuse radio emission in these clusters at 153 MHz, and provided flux density measurements of all the sources at this frequency. Our new flux density measurements, coupled with the existing data at higher frequencies, allow us to study the total spectrum of the halos and relic over at least one order of magnitude in frequency. Our images confirm the presence of a very steep "diffuse component" in Abell 1682. We found that the spectrum of the relic in Abell 521 can be fitted by a single power-law with $\alpha=1.45\pm0.02$ from 153 MHz to 5 GHz. Moreover, we confirm that the halos in Abell 521 and Abell 697 have a very steep spectrum, with $\alpha=1.8-1.9$ and $\alpha=1.52\pm0.05$ respectively. Even with the inclusion of the 153 MHz flux density information it is impossible to discriminate between power-law and curved spectra, as derived from homogeneous turbulent re-acceleration. The latter are favored on the basis of simple energetic arguments, and we expect that LOFAR will finally unveil the shape of the spectra of radio halos below 100 MHz, thus providing clues on their origin.Comment: 11 pages, 6 figures, 3 tables, accepted for publication in A&

Hsp60 quantification in human gastric mucosa shows differences between pathologies with various degrees of proliferation and malignancy grade

Background: Stomach diseases are an important sector of gastroenterology, including proliferative benign; premalignant; and malignant pathologies of the gastric mucosa, such as gastritis, hyperplastic polyps, metaplasia, dysplasia, and adenocarcinoma. There are data showing quantitative changes in chaperone system (CS) components in inflammatory pathologies and tumorigenesis, but their roles are poorly understood, and information pertaining to the stomach is scarce. Here, we report our findings on one CS component, the chaperone Hsp60, which we studied first considering its essential functions inside and outside mitochondria. Methods: We performed immunohistochemical experiments for Hsp60 in different samples of gastric mucosa. Results: The data obtained by quantitative analysis showed that the average percentages of Hsp60 were of 32.8 in normal mucosa; 33.5 in mild-to-moderate gastritis; 51.8 in severe gastritis; 58.5 in hyperplastic polyps; 67.0 in intestinal metaplasia; 89.4 in gastric dysplasia; and 92.5 in adenocarcinomas. Noteworthy were: (i) the difference between dysplasia and adenocarcinoma with the other pathologies; (ii) the progressive increase in Hsp60 from gastritis to hyperplastic polyp, gastric dysplasia, and gastric carcinoma; and (iii) the correlation of Hsp60 levels with histological patterns of cell proliferation and, especially, with tissue malignancy grades. Conclusions: This trend likely reflects the mounting need for cells for Hsp60 as they progress toward malignancy and is a useful indicator in differential diagnosis, as well as the call for research on the mechanisms underpinning the increase in Hsp60 and its possible roles in carcinogenesis

How Aromatic Fluorination Exchanges the Interaction Role of Pyridine with Carbonyl Compounds: The Formaldehyde Adduct

The rotational spectrum of the weakly bound complex pentafluoropyridine⋅⋅⋅formaldehyde has been investigated using Fourier transform microwave spectroscopy. From the analysis of the rotational parameters of the parent species and of the 13C and 15N isotopologues, the structural arrangement of the adduct has been unambiguously established. The full ring fluorination of pyridine has a dramatic effect on its binding properties: It alters the electron density distribution at the π-cloud of pyridine creating a π-hole and changing its electron donor-acceptor capabilities. In the complex, formaldehyde lies above the aromatic ring with one of the oxygen lone pairs, as conventionally envisaged, pointing toward its centre. This lone pair⋅⋅⋅π-hole interaction, reinforced by a weak C−H⋅⋅⋅N interaction, indicates an exchange of the electron-acceptor roles of both molecules when compared to the pyridine⋅⋅⋅formaldehyde adduct. Tunnelling doublets due to the internal rotation of formaldehyde have also been observed and analysed leading to a discussion on the competition between lone pair⋅⋅⋅π-hole and π⋅⋅⋅π stacking interactions