6,323 research outputs found
Detection of Ralstonia solanacearum (Rs) in latently infected potato stem extracts by post-enrichment qPCR.
A sensitive method for detection of Ralstonia solanacearum in latently infected potato stem extracts has been achieved by previous enrichment procedure followed by Real time PCR (qPCR). Sensitivity of qPCR before and after enrichment (48h of incubation of the stem extract with modified SMSA broth at 30oC) was compared with other techniques such as NCM-ELISA and R.solanacearum isolation in Kelmanâs medium. Before enrichment procedure, 174.6 cells/ml were detected in Kelmanâs medium, 1.71 x 106 cells/ml by NCM-ELISA and 1.29x105 cells/ by qPCR. After enrichment, sensitivities of post enrichment qPCR, NCM- ELISA and isolation on modified Kelmanâs medium were similar. As few as 6.56 cells/ml were detected in latently infected potato stem extracts. Serial dilutions of naturally-infected stem extract before enrichment allowed the quantification of populations of R. solanacearum in each stem extract. Post âenrichment qPCR combines the advantages of high sensitivity, ease and speed, but it requires expensive laboratory equipment. Thus it can be used by seed and breeding programmes in developing countries only to confirm positive results obtained by serological tests used for seed quality control and for assessing susceptibility of breeding lines to bacterial wilt
Meta-model Pruning
Large and complex meta-models such as those of Uml and its profiles are growing due to modelling and inter-operability needs of numerous\ud
stakeholders. The complexity of such meta-models has led to coining\ud
of the term meta-muddle. Individual users often exercise only a small\ud
view of a meta-muddle for tasks ranging from model creation to construction\ud
of model transformations. What is the effective meta-model that represents\ud
this view? We present a flexible meta-model pruning algorithm and\ud
tool to extract effective meta-models from a meta-muddle. We use\ud
the notion of model typing for meta-models to verify that the algorithm\ud
generates a super-type of the large meta-model representing the meta-muddle.\ud
This implies that all programs written using the effective meta-model\ud
will work for the meta-muddle hence preserving backward compatibility.\ud
All instances of the effective meta-model are also instances of the\ud
meta-muddle. We illustrate how pruning the original Uml metamodel\ud
produces different effective meta-models
Seeding hESCs to achieve optimal colony clonality
Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) have promising clinical applications which often rely on clonally-homogeneous cell populations. To achieve this, it is important to ensure that each colony originates from a single founding cell and to avoid subsequent merging of colonies during their growth. Clonal homogeneity can be obtained with low seeding densities; however, this leads to low yield and viability. It is therefore important to quantitatively assess how seeding density affects clonality loss so that experimental protocols can be optimised to meet the required standards. Here we develop a quantitative framework for modelling the growth of hESC colonies from a given seeding density based on stochastic exponential growth. This allows us to identify the timescales for colony merges and over which colony size no longer predicts the number of founding cells. We demonstrate the success of our model by applying it to our own experiments of hESC colony growth; while this is based on a particular experimental set-up, the model can be applied more generally to other cell lines and experimental conditions to predict these important timescales
OptEEmAL: Decision-Support Tool for the Design of Energy Retrofitting Projects at District Level
Designing energy retrofitting actions poses an elevated number of problems, as the definition of the baseline, selection of indicators to measure performance, modelling, setting objectives, etc. This is time-consuming and it can result in a number of inaccuracies, leading to inadequate decisions. While these problems are present at building level, they are multiplied at district level, where there are complex interactions to analyse, simulate and improve. OptEEmAL proposes a solution as a decision-support tool for the design of energy retrofitting projects at district level. Based on specific input data (IFC(s), CityGML, etc.), the platform will automatically simulate the baseline scenario and launch an optimisation process where a series of Energy Conservation Measures (ECMs) will be applied to this scenario. Its performance will be evaluated through a holistic set of indicators to obtain the best combination of ECMs that complies with user's objectives. A great reduction in time and higher accuracy in the models are experienced, since they are automatically created and checked. A subjective problem is transformed into a mathematical problem; it simplifies it and ensures a more robust decision-making. This paper will present a case where the platform has been tested.This research work has been partially funded by the European Commission though the European Unionâs Horizon 2020 Research and Innovation Programme under grant agreement No 680676. All related information to the project is available at https://www.opteemal-project.eu
Management of incidentally detected heart murmurs in dogs and cats
A dog or a cat has an incidentally detected heart murmur if the murmur is an unexpected discovery during a veterinary consultation that was not initially focused on the cardiovascular system. This document presents approaches for managing dogs and cats that have incidentally-detected heart murmurs, with an emphasis on murmur characteristics, signalment profiling, and multifactorial decision-making to choose an optimal course for a given patient
History of degenerative spondylolisthesis: From anatomical description to surgical management
This review of the historical medical literature aimed at understanding the evolution of surgical management of degenerative spondylolisthesis over time. The Medic@, IndexCat and Gallica historical databases and PubMed and Embase medical databases were used, with several search-terms, exploring the years 1700-2018. Data from anatomical, biomechanical, pathophysiological and surgical studies were compiled. In total, 150 documents were obtained, dating from 1782 to 2018: 139 from PubMed, 1 from Medic@, 7 from IndexCat, and 3 from Gallica. The review thus ranges in time from (1) description of the first clinical cases by several authors in Europe (1782), (2) the identification of a distinct entity by MacNab (1963), and (3) surgical management by the emerging discipline of minimally invasive spine surgery, to its subsequent evolution up to the present day. Spondylolisthesis is a frequent condition potentially responsible for a variety of functional impairments. Understanding and surgical management have progressed since the 20th century. Historically, the first descriptions of treatments concerned only spondylolisthesis associated with spondylolysis, especially in young adults. More recently, there has been progress in the understanding of the disease in elderly people, with the recognition of degenerative spondylolisthesis. New technologies and surgical techniques, aided by advances in supportive care, now provide spine surgeons with powerful treatment tools. Better knowledge of the evolution of surgery throughout history should enable better understanding of current approaches and concepts for treating degenerative spondylolisthesis
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Keys to academic success for under-represented minority young investigators: recommendations from the Research in Academic Pediatrics Initiative on Diversity (RAPID) National Advisory Committee.
BackgroundAlthough Latinos, African-Americans, and American Indians/Alaska Natives comprise 34% of Americans, these under-represented minorities (URMs) account for only 7% of US medical-school faculty. Even when URMs become faculty, they face many substantial challenges to success. Little has been published, however, on keys to academic success for URM young faculty investigators.MethodsThe Research in Academic Pediatrics Initiative on Diversity (RAPID) goal is to enhance the professional advancement of URM junior faculty pursuing research careers in general academic pediatrics. One important RAPID component is the annual mentoring/career-development conference, which targets URM residents, fellows, and junior faculty, and has included 62 URM participants since its 2013 inception. A conference highlight is the panel discussion on keys to academic success for URM young investigators, conducted by the RAPID National Advisory Committee, a diverse group of leading senior researchers. The article aim was to provide a guide to academic success for URM young investigators using the 2018 RAPID Conference panel discussion. A modified Delphi technique was used to provide a systematic approach to obtaining answers to six key questions using an expert panel: the single most important key to success for URM young investigators; ensuring optimal mentorship; how to respond when patients/families say, "I don't want you to see my child because you are ____"; best strategies for maximizing funding success; how to balance serving on time-consuming committees with enough time to advance research/career objectives; and the single thing you wish someone had told you which would have substantially enhanced your success early on.Results/conclusionsThis is the first published practical guide on keys to academic success for URM young investigators. Identified keys to success included having multiple mentors, writing prolifically, being tenaciously persistent, having mentors who are invested in you, dealing with families who do not want you to care for their child because of your race/ethnicity by seeking to understand the reasons and debriefing with colleagues, seeking non-traditional funding streams, balancing committee work with having enough time to advance one's research and career by using these opportunities to generate scholarly products, and asking for all needed resources when negotiating for new jobs
Cardiovascular-renal axis disorders in the domestic dog and cat: a veterinary consensus statement
OBJECTIVES
There is a growing understanding of the complexity of interplay between renal and cardiovascular systems in both health and disease. The medical profession has adopted the term "cardiorenal syndrome" (CRS) to describe the pathophysiological relationship between the kidney and heart in disease. CRS has yet to be formally defined and described by the veterinary profession and its existence and importance in dogs and cats warrant investigation. The CRS Consensus Group, comprising nine veterinary cardiologists and seven nephrologists from Europe and North America, sought to achieve consensus around the definition, pathophysiology, diagnosis and management of dogs and cats with "cardiovascular-renal disorders" (CvRD). To this end, the Delphi formal methodology for defining/building consensus and defining guidelines was utilised.
METHODS
Following a literature review, 13 candidate statements regarding CvRD in dogs and cats were tested for consensus, using a modified Delphi method. As a new area of interest, well-designed studies, specific to CRS/CvRD, are lacking, particularly in dogs and cats. Hence, while scientific justification of all the recommendations was sought and used when available, recommendations were largely reliant on theory, expert opinion, small clinical studies and extrapolation from data derived from other species.
RESULTS
Of the 13 statements, 11 achieved consensus and 2 did not. The modified Delphi approach worked well to achieve consensus in an objective manner and to develop initial guidelines for CvRD.
DISCUSSION
The resultant manuscript describes consensus statements for the definition, classification, diagnosis and management strategies for veterinary patients with CvRD, with an emphasis on the pathological interplay between the two organ systems. By formulating consensus statements regarding CvRD in veterinary medicine, the authors hope to stimulate interest in and advancement of the understanding and management of CvRD in dogs and cats. The use of a formalised method for consensus and guideline development should be considered for other topics in veterinary medicine
Seyfert's Sextet: A Slowly Dissolving Stephan's Quintet?
We present a multiwavelength study of the highly evolved compact galaxy group
known as Seyfert's Sextet (HCG79: SS). We interpret SS as a 2-3 Gyr more
evolved analog of Stephan's Quintet (HCG92: SQ). We postulate that SS formed by
sequential acquisition of 4-5 primarily late-type field galaxies. Four of the
five galaxies show an early-type morphology which is likely the result of
secular evolution driven by gas stripping. Stellar stripping has produced a
massive/luminous halo and embedded galaxies that are overluminous for their
size. These are interpreted as remnant bulges of the accreted spirals. H79d
could be interpreted as the most recent intruder being the only galaxy with an
intact ISM and uncertain evidence for tidal perturbation. In addition to
stripping activity we find evidence for past accretion events. H79b (NGC6027)
shows a strong counter-rotating emission line component interpreted as an
accreted dwarf spiral. H79a shows evidence for an infalling component of gas
representing feedback or possible cross fueling by H79d. The biggest challenge
to this scenario involves the low gas fraction in the group. If SS formed from
normal field spirals then much of the gas is missing. Finally, despite its
advanced stage of evolution, we find no evidence for major mergers and infer
that SS (and SQ) are telling us that such groups coalesce via slow dissolution.Comment: 70 pages, 19 figures, 15 tables - accepted for publication in the
Astronomical Journa
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