Characterization of cell wall degrading enzymes from Chrysosporium lucknowense C1 and their use to degrade sugar beet pulp

Key words: Pectin, arabinan, biorefinery, mode of action, branched arabinose oligomers, ferulic acid esterase, arabinohydrolase, pretreatment Sugar beet pulp is the cellulose and pectin-rich debris remaining after sugar extraction from sugar beets. In order to use sugar beet pulp for biorefinery purposes, these carbohydrates need to be degraded to fermentable monosaccharides.The influence of six mild sulfuric acid or water pretreatments at different temperatures on the enzymatic degradability of sugar beet pulp was examined. An optimal pretreatment of 15 min at 140 °C in water greatly facilitates the subsequent enzymatic cellulose degradation. Enzymatic degradation of pectin requires an array of different enzyme activities. To this end, cell wall degrading enzymes of the filamentous fungus Chrysosporium lucknowense C1 (C1) were purified and characterized. Four arabinohydrolases from C1 were characterized that released up to 80% of the arabinose present in sugar beet arabinan as monomers. A combination of three arabinohydrolases also released unknown branched arabinose oligomers, which were purified and identified using NMR analysis. With the help of these complex arabinose oligomers the mode of action of four C1 α-L-arabinohydrolases was determined to enable controlled and tailored degradation of arabinan. Complete degradation of sugar beet pulp also requires ferulic acid esterase activity. Three C1 ferulic acid esterases were purified and characterized, of which one could release up to 60% of ferulic acid from feruloylated sugar beet pectin oligomers, whereas the other two were more active toward feruloylated arabinoxylan. </p

Mixing Effects in the Crystallization of Supercooled Quantum Binary Liquids

By means of Raman spectroscopy of liquid microjets we have investigated the crystallization process of supercooled quantum liquid mixtures composed of parahydrogen (pH$_2$) diluted with small amounts of up to 5\% of either neon or orthodeuterium (oD$_2$), and of oD$_2$ diluted with either Ne or pH$_2$. We show that the introduction of Ne impurities affects the crystallization kinetics in both the pH$_2$-Ne and oD$_2$-Ne mixtures in terms of a significant reduction of the crystal growth rate, similarly to what found in our previous work on supercooled pH$_2$-oD$_2$ liquid mixtures [M. K\"uhnel et {\it al.}, Phys. Rev. B \textbf{89}, 180506(R) (2014)]. Our experimental results, in combination with path-integral simulations of the supercooled liquid mixtures, suggest in particular a correlation between the measured growth rates and the ratio of the effective particle sizes originating from quantum delocalization effects. We further show that the crystalline structure of the mixture is also affected to a large extent by the presence of the Ne impurities, which likely initiate the freezing process through the formation of Ne crystallites.Comment: 19 pages, 7 figures, submitted to J. Chem. Phy

Interatomic Coulombic Decay following Photoionization of the Helium Dimer: Observation of Vibrational Structure

Using synchrotron radiation we simultaneously ionize and excite one helium atom of a helium dimer (He_2) in a shakeup process. The populated states of the dimer ion (i.e. He^[*+](n = 2; 3)-He) are found to deexcite via interatomic coulombic decay. This leads to the emission of a second electron from the neutral site and a subsequent coulomb explosion. In this letter we present a measurement of the momenta of fragments that are created during this reaction. The electron energy distribution and the kinetic energy release of the two He^+ ions show pronounced oscillations which we attribute to the structure of the vibrational wave function of the dimer ion.Comment: 8 pages, 5 figure

Single photon double ionization of the helium dimer

We show that a single photon can ionize the two helium atoms of the helium dimer in a distance up to 10 {\deg}A. The energy sharing among the electrons, the angular distributions of the ions and electrons as well as comparison with electron impact data for helium atoms suggest a knock-off type double ionization process. The Coulomb explosion imaging of He_2 provides a direct view of the nuclear wave function of this by far most extended and most diffuse of all naturally existing molecules.Comment: 10 pages, 5 figure

Formation of phase lags at the cyclotron energies in the pulse profiles of magnetized, accreting neutron stars

Context: Accretion-powered X-ray pulsars show highly energy-dependent and complex pulse-profile morphologies. Significant deviations from the average pulse profile can appear, in particular close to the cyclotron line energies. These deviations can be described as energy-dependent phase lags, that is, as energy-dependent shifts of main features in the pulse profile. Aims: Using a numerical study we explore the effect of cyclotron resonant scattering on observable, energy-resolved pulse profiles. Methods: We generated the observable emission as a function of spin phase, using Monte Carlo simulations for cyclotron resonant scattering and a numerical ray-tracing routine accounting for general relativistic light-bending effects on the intrinsic emission from the accretion columns. Results: We find strong changes in the pulse profile coincident with the cyclotron line energies. Features in the pulse profile vary strongly with respect to the average pulse profile with the observing geometry and shift and smear out in energy additionally when assuming a non-static plasma. Conclusions: We demonstrate how phase lags at the cyclotron energies arise as a consequence of the effects of angular redistribution of X-rays by cyclotron resonance scattering in a strong magnetic field combined with relativistic effects. We also show that phase lags are strongly dependent on the accretion geometry. These intrinsic effects will in principle allow us to constrain a system's accretion geometry.Comment: 4 pages, 4 figures; updated reference lis

Triangulations and Severi varieties

We consider the problem of constructing triangulations of projective planes over Hurwitz algebras with minimal numbers of vertices. We observe that the numbers of faces of each dimension must be equal to the dimensions of certain representations of the automorphism groups of the corresponding Severi varieties. We construct a complex involving these representations, which should be considered as a geometric version of the (putative) triangulations

Modelling phagosomal lipid networks that regulate actin assembly

<p>Abstract</p> <p>Background</p> <p>When purified phagosomes are incubated in the presence of actin under appropriate conditions, microfilaments start growing from the membrane in a process that is affected by ATP and the lipid composition of the membrane. Isolated phagosomes are metabolically active organelles that contain enzymes and metabolites necessary for lipid interconversion. Hence, addition of ATP, lipids, and actin to the system alter the steady-state composition of the phagosomal membrane at the same time that the actin nucleation is initiated. Our aim was to model all these processes in parallel.</p> <p>Results</p> <p>We compiled detailed experimental data on the effects of different lipids and ATP on actin nucleation and we investigated experimentally lipid interconversion and ATP metabolism in phagosomes by using suitable radioactive compounds.</p> <p>In a first step, a complex lipid network interconnected by chemical reactions catalyzed by known enzymes was modelled in COPASI (Complex Pathway Simulator). However, several lines of experimental evidence indicated that only the phosphatidylinositol branch of the network was active, an observation that dramatically reduced the number of parameters in the model. The results also indicated that a lipid network-independent ATP-consuming activity should be included in the model. When this activity was introduced, the set of differential equations satisfactorily reproduced the experimental data. On the other hand, a molecular mechanism connecting membrane lipids, ATP, and the actin nucleation process is still missing. We therefore adopted a phenomenological (black-box) approach to represent the empirical observations. We proposed that lipids and ATP influence the dynamic interconversion between active and inactive actin nucleation sites. With this simple model, all the experimental data were satisfactorily fitted with a single positive parameter per lipid and ATP.</p> <p>Conclusion</p> <p>By establishing an active 'dialogue' between an initial complex model and experimental observations, we could narrow the set of differential equations and parameters required to characterize the time-dependent changes of metabolites influencing actin nucleation on phagosomes. For this, the global model was dissected into three sub-models: ATP consumption, lipid interconversion, and nucleation of actin on phagosomal membranes. This scheme allowed us to describe this complex system with a relatively small set of differential equations and kinetic parameters that satisfactorily reproduced the experimental data.</p

Test of the REX-RFQ and status of the front part of the REX-ISOLDE linac

For REX-ISOLDE (Radioactive beam EXperiments at ISOLDE/CERN), a test beamline is built up at the Garching Accelerator Lab. to perform He$^{1+}$-experiments with the RFQ, the matching (rebunching) section between RFQ and IH-DT-linac, the IH-structure and several electrostatic lenses of the REX-ISOLDE-mass separator. In a first step, the beamline is conceived for tests with the RFQ. This paper presents the parameters and the status of the REX-RFQ, the experimental setup and the particle dynamics simulations with the COSY infinity code for beam injection and beam analysis. Furthermore it shows the design and status of the mass separator, the IH- structure and the buncher section. (5 refs)

Critical Behaviour of Non-Equilibrium Phase Transitions to Magnetically Ordered States

We describe non-equilibrium phase transitions in arrays of dynamical systems with cubic nonlinearity driven by multiplicative Gaussian white noise. Depending on the sign of the spatial coupling we observe transitions to ferromagnetic or antiferromagnetic ordered states. We discuss the phase diagram, the order of the transitions, and the critical behaviour. For global coupling we show analytically that the critical exponent of the magnetization exhibits a transition from the value 1/2 to a non-universal behaviour depending on the ratio of noise strength to the magnitude of the spatial coupling.Comment: 4 pages, 5 figure