1,353 research outputs found

    Comparative genomics and mutagenesis analyses of choline metabolism in the marine Roseobacter clade

    Get PDF
    Choline is ubiquitous in marine eukaryotes and appears to be widely distributed in surface marine waters; however, its metabolism by marine bacteria is poorly understood. Here, using comparative genomics and molecular genetic approaches, we reveal that the capacity for choline catabolism is widespread in marine heterotrophs of the marine Roseobacter clade (MRC). Using the model bacterium Ruegeria pomeroyi, we confirm that the betA, betB and betC genes, encoding choline dehydrogenase, betaine aldehyde dehydrogenase and choline sulfatase, respectively, are involved in choline metabolism. The betT gene, encoding an organic solute transporter, was essential for the rapid uptake of choline but not glycine betaine (GBT). Growth of choline and GBT as a sole carbon source resulted in the re-mineralization of these nitrogen-rich compounds into ammonium. Oxidation of the methyl groups from choline requires formyltetrahydrofolate synthetase encoded by fhs in R.pomeroyi, deletion of which resulted in incomplete degradation of GBT. We demonstrate that this was due to an imbalance in the supply of reducing equivalents required for choline catabolism, which can be alleviated by the addition of formate. Together, our results demonstrate that choline metabolism is ubiquitous in the MRC and reveal the role of Fhs in methyl group oxidation in R.pomeroyi

    Spiral Magnets as Gapless Mott Insulators

    Full text link
    In the large UU limit, the ground state of the half-filled, nearest-neighbor Hubbard model on the triangular lattice is the three-sublattice antiferromagnet. In sharp contrast with the square-lattice case, where transverse spin-waves and charge excitations remain decoupled to all orders in t/Ut/U, it is shown that beyond leading order in t/Ut/U the three Goldstone modes on the triangular lattice are a linear combination of spin and charge. This leads to non-vanishing conductivity at any finite frequency, even though the magnet remains insulating at zero frequency. More generally, non-collinear spin order should lead to such gapless insulating behavior.Comment: 10 pages, REVTEX 3.0, 3 uuencoded postscript figures, CRPS-94-0

    Soils of the Hawaii Agricultural Experiment Station, University of Hawaii: Soil Survey, Laboratory Data, and Soil Descriptions

    Get PDF
    Benchmark Soils Project technical report 4Soils of 16 research sites in the University of Hawaii's agricultural research station network are described in detail. The sites are on the islands of Kauai, Oahu, Maui, and Hawaii. Soil series in the soil orders Mollisols, Oxisols, Vertisols, Inceptisols, Ultisols, Histisols, and Entisols are described. Soil maps of the stations are shown. Tables provide a soil profile description and laboratory data for the predominant soil series at each site, the text discusses the characteristics of the soil types within the series, along with their capability classifications, and related soils or variants at the sites are described

    Competition between spin and charge polarized states in nanographene ribbons with zigzag edges

    Full text link
    Effects of the nearest neighbor Coulomb interaction on nanographene ribbons with zigzag edges are investigated using the extended Hubbard model within the unrestricted Hartree-Fock approximation. The nearest Coulomb interaction stabilizes a novel electronic state with the opposite electric charges separated and localized along both edges, resulting in a finite electric dipole moment pointing from one edge to the other. This charge-polarized state competes with the peculiar spin-polarized state caused by the on-site Coulomb interaction and is stabilized by an external electric field.Comment: 4 pages; 4 figures; accepted for publication in Phys. Rev. B; related Web site: http://staff.aist.go.jp/k.harigaya/index_E.htm

    Design of a five-axis ultra-precision micro-milling machine—UltraMill. Part 1: Holistic design approach, design considerations and specifications

    Get PDF
    High-accuracy three-dimensional miniature components and microstructures are increasingly in demand in the sector of electro-optics, automotive, biotechnology, aerospace and information-technology industries. A rational approach to mechanical micro machining is to develop ultra-precision machines with small footprints. In part 1 of this two-part paper, the-state-of-the-art of ultra-precision machines with micro-machining capability is critically reviewed. The design considerations and specifications of a five-axis ultra-precision micro-milling machine—UltraMill—are discussed. Three prioritised design issues: motion accuracy, dynamic stiffness and thermal stability, formulate the holistic design approach for UltraMill. This approach has been applied to the development of key machine components and their integration so as to achieve high accuracy and nanometer surface finish

    Notch signaling during human T cell development

    Get PDF
    Notch signaling is critical during multiple stages of T cell development in both mouse and human. Evidence has emerged in recent years that this pathway might regulate T-lineage differentiation differently between both species. Here, we review our current understanding of how Notch signaling is activated and used during human T cell development. First, we set the stage by describing the developmental steps that make up human T cell development before describing the expression profiles of Notch receptors, ligands, and target genes during this process. To delineate stage-specific roles for Notch signaling during human T cell development, we subsequently try to interpret the functional Notch studies that have been performed in light of these expression profiles and compare this to its suggested role in the mouse

    Total energy calculation of high pressure selenium: The origin of incommensurate modulations in Se-IV and the instability of proposed Se-II

    Full text link
    We present calculation of the high pressure crystal structures in selenium, including rational approximants to the recently reported incommensurate phases. We show how the incommensurate phases can be intuitively explained in terms of imaginary phonon frequencies arising from Kohn anomalies in the putative undistorted phase. We also find inconsistencies between the calculated and experimental Se-II phase - the calculations show it to be a metastable metal while the experiment finds a stable semiconductor. We propose that the experimentally reported structure is probably in error.Comment: 4 pages 4 figure

    Resolvent estimates for normally hyperbolic trapped sets

    Full text link
    We give pole free strips and estimates for resolvents of semiclassical operators which, on the level of the classical flow, have normally hyperbolic smooth trapped sets of codimension two in phase space. Such trapped sets are structurally stable and our motivation comes partly from considering the wave equation for Kerr black holes and their perturbations, whose trapped sets have precisely this structure. We give applications including local smoothing effects with epsilon derivative loss for the Schr\"odinger propagator as well as local energy decay results for the wave equation.Comment: Further changes to erratum correcting small problems with Section 3.5 and Lemma 4.1; this now also corrects hypotheses, explicitly requiring trapped set to be symplectic. Erratum follows references in this versio
    corecore