Gravity and rotation drag the magnetic field in high-mass star formation

The formation of hot stars out of the cold interstellar medium lies at the heart of astrophysical research. Understanding the importance of magnetic fields during star formation remains a major challenge. With the advent of the Atacama Large Millimeter Array, the potential to study magnetic fields by polarization observations has tremendously progressed. However, the major question remains how much magnetic fields shape the star formation process or whether gravity is largely dominating. Here, we show that for the high-mass star-forming region G327.3 the magnetic field morphology appears to be dominantly shaped by the gravitational contraction of the central massive gas core where the star formation proceeds. We find that in the outer parts of the region, the magnetic field is directed toward the gravitational center of the region. Filamentary structures feeding the central core exhibit U-shaped magnetic field morphologies directed toward the gravitational center as well, again showing the gravitational drag toward the center. The inner part then shows rotational signatures, potentially associated with an embedded disk, and there the magnetic field morphology appears to be rotationally dominated. Hence, our results demonstrate that for this region gravity and rotation are dominating the dynamics and shaping the magnetic field morphology.Comment: 10 pages, 4 figures, accepted for the Astrophysical Journal, also available at https://www2.mpia-hd.mpg.de/homes/beuther/papers.htm

Fragmentation and disk formation in high-mass star formation: the IRAM large program CORE

Interstellar matter and star formatio

Density distributions, magnetic field structures and fragmentation in high-mass star formation

Methods: Observing the large pc-scale Stokes I mm dust continuum emission with the IRAM 30m telescope and the intermediate-scale (<0.1pc) polarized submm dust emission with the Submillimeter Array toward a sample of 20 high-mass star-forming regions allows us to quantify the dependence of the fragmentation behaviour of these regions depending on the density and magnetic field structures. Results: We infer density distributions n~r^{-p} of the regions with typical power-law slopes p around ~1.5. There is no obvious correlation between the power-law slopes of the density structures on larger clump scales (~1pc) and the number of fragments on smaller core scales (<0.1pc). Comparing the large-scale single-dish density profiles to those derived earlier from interferometric observations at smaller spatial scales, we find that the smaller-scale power-law slopes are steeper, typically around ~2.0. The flattening toward larger scales is consistent with the star-forming regions being embedded in larger cloud structures that do not decrease in density away from a particular core. Regarding the magnetic field, for several regions it appears aligned with filamentary structures leading toward the densest central cores. Furthermore, we find different polarization structures with some regions exhibiting central polarization holes whereas other regions show polarized emission also toward the central peak positions. Nevertheless, the polarized intensities are inversely related to the Stokes I intensities. We estimate magnetic field strengths between ~0.2 and ~4.5mG, and we find no clear correlation between magnetic field strength and the fragmentation level of the regions. Comparison of the turbulent to magnetic energies shows that they are of roughly equal importance in this sample. The mass-to-flux ratios range between ~2 and ~7, consistent with collapsing star-forming regions.Comment: Accepted for Astronomy & Astrophysics, 14 pages, 14 figures plus appendices, also download option at https://www2.mpia-hd.mpg.de/homes/beuther/papers.htm

From clump to disc scales in W3 IRS4 A case study of the IRAM NOEMA large programme CORE

Context. High-mass star formation typically takes place in a crowded environment, with a higher likelihood of young forming stars affecting and being affected by their surroundings and neighbours, as well as links between different physical scales affecting the outcome. However, observational studies are often focused on either clump or disc scales exclusively. Aims. We explore the physical and chemical links between clump and disc scales in the high-mass star formation region W3 IRS4, a region that contains a number of different evolutionary phases in the high-mass star formation process, as a case-study for what can be achieved as part of the IRAM NOrthern Extended Millimeter Array (NOEMA) large programme named CORE: “Fragmentation and disc formation in high-mass star formation”. Methods. We present 1.4 mm continuum and molecular line observations with the IRAM NOEMA interferometer and 30 m telescope, which together probe spatial scales from ~0.3−20′′ (600−40 000 AU or 0.003−0.2 pc at 2 kpc, the distance to W3). As part of our analysis, we used XCLASS to constrain the temperature, column density, velocity, and line-width of the molecular emission lines. Results. The W3 IRS4 region includes a cold filament and cold cores, a massive young stellar object (MYSO) embedded in a hot core, and a more evolved ultra-compact (UC)H II region, with some degree of interaction between all components of the region that affects their evolution. A large velocity gradient is seen in the filament, suggesting infall of material towards the hot core at a rate of 10−3−10−4 M⊙ yr−1, while the swept up gas ring in the photodissociation region around the UCH II region may be squeezing the hot core from the other side. There are no clear indications of a disc around the MYSO down to the resolution of the observations (600 AU). A total of 21 molecules are detected, with the abundances and abundance ratios indicating that many molecules were formed in the ice mantles of dust grains at cooler temperatures, below the freeze-out temperature of CO (≲35 K). This contrasts with the current bulk temperature of ~50 K, which was obtained from H2CO. Conclusions. CORE observations allow us to comprehensively link the different structures in the W3 IRS4 region for the first time. Our results argue that the dynamics and environment around the MYSO W3 IRS4 have a significant impact on its evolution. This context would be missing if only high resolution or continuum observations were available

Fragmentation and disk formation during high-mass star formation: The IRAM NOEMA (Northern Extended Millimeter Array) large program CORE

Aims: We aim to understand the fragmentation as well as the disk formation, outflow generation and chemical processes during high-mass star formation on spatial scales of individual cores. Methods: Using the IRAM Northern Extended Millimeter Array (NOEMA) in combination with the 30m telescope, we have observed in the IRAM large program CORE the 1.37mm continuum and spectral line emission at high angular resolution (~0.4'') for a sample of 20 well-known high-mass star-forming regions with distances below 5.5kpc and luminosities larger than 10^4Lsun. Results: We present the overall survey scope, the selected sample, the observational setup and the main goals of CORE. Scientifically, we concentrate on the mm continuum emission on scales on the order of 1000AU. We detect strong mm continuum emission from all regions, mostly due to the emission from cold dust. The fragmentation properties of the sample are diverse. We see extremes where some regions are dominated by a single high-mass core whereas others fragment into as many as 20 cores. A minimum-spanning-tree analysis finds fragmentation at scales on the order of the thermal Jeans length or smaller suggesting that turbulent fragmentation is less important than thermal gravitational fragmentation. The diversity of highly fragmented versus singular regions can be explained by varying initial density structures and/or different initial magnetic field strengths. Conclusions: The smallest observed separations between cores are found around the angular resolution limit which indicates that further fragmentation likely takes place on even smaller spatial scales. The CORE project with its numerous spectral line detections will address a diverse set of important physical and chemical questions in the field of high-mass star formation

A transcriptome analysis of mitten crab testes (Eriocheir sinensis)

The identification of expressed genes involved in sexual precocity of the mitten crab (Eriocheir sinensis) is critical for a better understanding of its reproductive development. To this end, we constructed a cDNA library from the rapid developmental stage of testis of E. sinensis and sequenced 3,388 randomly picked clones. After processing, 2,990 high-quality expressed sequence tags (ESTs) were clustered into 2,415 unigenes including 307 contigs and 2,108 singlets, which were then compared to the NCBI non-redundant (nr) protein and nucleotide (nt) database for annotation with Blastx and Blastn, respectively. After further analysis, 922 unigenes were obtained with concrete annotations and 30 unigenes were found to have functions possibly related to the process of reproduction in male crabs – six transcripts relevant to spermatogenesis (especially Cyclin K and RecA homolog DMC1), two transcripts involved in nuclear protein transformation, two heat-shock protein genes, eleven transcription factor genes (a series of zinc-finger proteins), and nine cytoskeleton protein-related genes. Our results, besides providing valuable information related to crustacean reproduction, can also serve as a base for future studies of reproductive and developmental biology

Clustered star formation at early evolutionary stages. Physical and chemical analysis of the young star-forming regions ISOSS J22478+6357 and ISOSS J23053+5953

We aim to characterize the physical and chemical properties of fragmented cores during the earliest evolutionary stages in the very young star-forming regions ISOSS J22478+6357 and ISOSS J23053+5953. NOEMA 1.3 mm data are used in combination with archival mid- and far-infrared observations to construct and fit the SEDs of individual fragmented cores. The radial density profiles are inferred from the 1.3 mm continuum visibility profiles and the radial temperature profiles are estimated from H2CO rotation temperature maps. Molecular column densities are derived with the line fitting tool XCLASS. The physical and chemical properties are combined by applying the physical-chemical model MUSCLE in order to constrain the chemical timescales of a few line-rich cores. The morphology and spatial correlations of the molecular emission are analyzed using the HOG method. The mid-infrared data show that both regions contain a cluster of young stellar objects. Bipolar molecular outflows are observed in the CO 2-1 transition toward the strong mm cores indicating protostellar activity. We find strong molecular emission of SO, SiO, H2CO, and CH3OH in locations which are not associated with the mm cores. These shocked knots can be either associated with the bipolar outflows or, in the case of ISOSS J23053+5953, with a colliding flow that creates a large shocked region between the mm cores. The mean chemical timescale of the cores is lower (20 000 yr) compared to that of the sources of the more evolved CORE sample (60 000 yr). With the HOG method, we find that the spatial emission of species tracing the extended emission and of shock-tracing molecules are well correlated within transitions of these groups

Transcriptional Regulation of Rod Photoreceptor Homeostasis Revealed by In Vivo NRL Targetome Analysis

A stringent control of homeostasis is critical for functional maintenance and survival of neurons. In the mammalian retina, the basic motif leucine zipper transcription factor NRL determines rod versus cone photoreceptor cell fate and activates the expression of many rod-specific genes. Here, we report an integrated analysis of NRL-centered gene regulatory network by coupling chromatin immunoprecipitation followed by high-throughput sequencing (ChIP–Seq) data from Illumina and ABI platforms with global expression profiling and in vivo knockdown studies. We identified approximately 300 direct NRL target genes. Of these, 22 NRL targets are associated with human retinal dystrophies, whereas 95 mapped to regions of as yet uncloned retinal disease loci. In silico analysis of NRL ChIP–Seq peak sequences revealed an enrichment of distinct sets of transcription factor binding sites. Specifically, we discovered that genes involved in photoreceptor function include binding sites for both NRL and homeodomain protein CRX. Evaluation of 26 ChIP–Seq regions validated their enhancer functions in reporter assays. In vivo knockdown of 16 NRL target genes resulted in death or abnormal morphology of rod photoreceptors, suggesting their importance in maintaining retinal function. We also identified histone demethylase Kdm5b as a novel secondary node in NRL transcriptional hierarchy. Exon array analysis of flow-sorted photoreceptors in which Kdm5b was knocked down by shRNA indicated its role in regulating rod-expressed genes. Our studies identify candidate genes for retinal dystrophies, define cis-regulatory module(s) for photoreceptor-expressed genes and provide a framework for decoding transcriptional regulatory networks that dictate rod homeostasis

Disk fragmentation in high-mass star formation. High-resolution observations towards AFGL 2591-VLA 3

Increasing evidence suggests that, similar to their low-mass counterparts, high-mass stars form through a disk-mediated accretion process. At the same time, formation of high-mass stars still necessitates high accretion rates, and hence, high gas densities, which in turn can cause disks to become unstable against gravitational fragmentation. We study the kinematics and fragmentation of the disk around the high-mass star forming region AFGL 2591-VLA 3 which was hypothesized to be fragmenting based on the observations that show multiple outflow directions. We use a new set of high-resolution (0.19 arcsec) IRAM/NOEMA observations at 843 micron towards VLA 3 which allow us to resolve its disk, characterize the fragmentation, and study its kinematics. In addition to the 843 micron continuum emission, our spectral setup targets warm dense gas and outflow tracers such as HCN, HC$_3$N and SO$_2$, as well as vibrationally excited HCN lines. The high resolution continuum and line emission maps reveal multiple fragments with subsolar masses within the inner 1000 AU of VLA 3. Furthermore, the velocity field of the inner disk observed at 843 micron shows a similar behavior to that of the larger scale velocity field studied in the CORE project at 1.37 mm. We present the first observational evidence for disk fragmentation towards AFGL 2591-VLA 3, a source that was thought to be a single high-mass core. While the fragments themselves are low-mass, the rotation of the disk is dominated by the protostar with a mass of 10.3$\pm 1.8~M_{\odot}$. These data also show that NOEMA Band 4 can obtain the highest currently achievable spatial resolution at (sub-)mm wavelengths in observations of strong northern sources

Chemical complexity in high-mass star formation: An observational and modeling case study of the AFGL 2591 VLA 3 hot core

Aims. In order to understand the observed molecular diversity in high-mass star-forming regions, we have to determine the underlying physical and chemical structure of those regions at high angular resolution and over a range of evolutionary stages. We present a detailed observational and modeling study of the hot core VLA 3 in the high-mass star-forming region AFGL 2591, which is a target region of the NOrthern Extended Millimeter Array (NOEMA) large program CORE. Using NOEMA observations at 1.37 mm with an angular resolution of ~0."42 (1 400 au at 3.33 kpc), we derived the physical and chemical structure of the source. We modeled the observed molecular abundances with the chemical evolution code MUSCLE (MUlti Stage ChemicaL codE). Results. With the kinetic temperature tracers CH3CN and H2CO we observe a temperature distribution with a power-law index of q = 0.41+-0.08. Using the visibilities of the continuum emission we derive a density structure with a power-law index of p = 1.7+-0.1. The hot core spectra reveal high molecular abundances and a rich diversity in complex molecules. The majority of the molecules have an asymmetric spatial distribution around the forming protostar(s), which indicates a complex physical structure on scales < 1 400 au. Using MUSCLE, we are able to explain the observed molecular abundance of 10 out of 14 modeled species at an estimated hot core chemical age of ~21 100 years. In contrast to the observational analysis, our chemical modeling predicts a lower density power-law index of p < 1.4. Reasons for this discrepancy are discussed. Conclusions. Combining high spatial resolution observations with detailed chemical modeling allows us to derive a concise picture of the physical and chemical structure of the famous AFGL 2591 hot core. The next steps are to conduct a similar analysis for the whole CORE sample, and then use this analysis to constrain the chemical diversity in high-mass star formation to a much greater depth