Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast

Water is involved in all aspects of biological activity, both as a solvent and as a reactant. It is hypothesized that intracellular water is in a highly structured state due to the high concentrations of macromolecules in the cell and that this may change the activity of intracellular enzymes due to altered binding affinities and allosteric regulations. Here we first investigate the kinetics of two glycolytic enzymes in artificially crowded aqueous solutions and show that crowding does indeed change their kinetics. Based on our kinetic measurements we propose a new model of oscillating glycolysis that instead of Michaelis-Menten or Monod-Wyman-Changeux kinetics uses the Yang-Ling adsorption isotherm introduced by G. Ling in the frame of the Association-Induction (AI) hypothesis. Using this model, we can reproduce previous experimental observations of the coupling of glycolytic oscillations and intracellular water dynamics, e.g., (i) during the metabolic oscillations, the latter variable oscillates in phase with ATP activity, and (ii) the emergence of glycolytic oscillations largely depends on the extent of intracellular water dipolar relaxation in cells in the resting state. Our results support the view that the extent of intracellular water dipolar relaxation is regulated by the ability of cytoplasmic proteins to polarize intracellular water with the assistance of ATP, as suggested in the AI hypothesis. This hypothesis may be relevant to the interpretation of many other biological oscillators, including cell signalling processes.Fil: Thoke, Henrik S.. University Of Southern Denmark; DinamarcaFil: Bagatolli, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Olsen, Lars F.. University Of Southern Denmark; Dinamarc

Heat and extension at mid- and lower crustal levels of the Rio Grande rift

The process by which large amounts (50 to 200 percent) of crustal extension are produced was concisely described by W. Hamilton in 1982 and 1983. More recently, England, Sawyer, P. Morgan and others have moved toward quantifying models of lithospheric thinning by incorporating laboratory and theoretical data on rock rheology as a function of composition, temperature, and strain rate. Hamilton's description identifies three main crustal layers, each with a distinctive mechanical behavior; brittle fracturing and rotation in the upper crust, discontinuous ductile flow in the middle crust and laminar ductile flow in the lower crust. The temperature and composition dependent brittle-ductile transition essentially defines the diffuse boundary between upper and middle crust. It was concluded that the heat responsible for the highly ductile nature of the lower crust and the lensoidal and magma body structures at mid-crustal depths in the rift was infused into the crust by relatively modest ( 10 percent by mass) magmatic upwelling (feeder dikes) from Moho levels. Seismic velocity-versus-depth data, supported by gravity modeling and the fact that volumes of rift related volcanics are relatively modest ( 6000 cubic km) for the Rio Grande system, all imply velocities and densities too small to be consistent with a massive, composite, mafic intrusion in the lower crust

Cross-Dimensional relaxation in Bose-Fermi mixtures

We consider the equilibration rate for fermions in Bose-Fermi mixtures undergoing cross-dimensional rethermalization. Classical Monte Carlo simulations of the relaxation process are performed over a wide range of parameters, focusing on the effects of the mass difference between species and the degree of initial departure from equilibrium. A simple analysis based on Enskog's equation is developed and shown to be accurate over a variety of different parameter regimes. This allows predictions for mixtures of commonly used alkali atoms.Comment: 7 pages, 4 figures, uses Revtex 4. This is a companion paper to [PRA 70, 021601(R) (2004)] (cond-mat/0405419

The table mountain 8-mm-wavelength interferometer

The system components, performance, and calibration of two element radio interferometer operating at 8.33 mm wavelength are discussed. The interferometer employs a 5.5 m and a 3 m diameter antenna on an east-west baseline of 60 or 120 m, yielding fringe spacings at transit of 28 or 14 in. respectively. The broad intermediate frequency bandpass of 100 to 350 MHz and the system noise temperature of 500 K provide high sensitivity for the measurement of continuum sources. The interferometer has been used for high resolution studies of the planets and the Sun, and it is currently being adapted to study solar flare emissions at high spatial and time resolution

Quadripartite continuous-variable entanglement via quadruply concurrent downconversion

We investigate an intra-cavity coupled down-conversion scheme to generate quadripartite entanglement using concurrently resonant nonlinearities. We verify that quadripartite entanglement is present in this system by calculating the output fluctuation spectra and then considering violations of optimized inequalities of the van Loock-Furusawa type. The entanglement characteristics both above and below the oscillation threshold are considered. We also present analytic solutions for the quadrature operators and the van Loock-Furusawa correlations in the undepleted pump approximation.Comment: 9 pages, 5 figure

Tripartite entanglement and threshold properties of coupled intracavity downconversion and sum-frequency generation

The process of cascaded downconversion and sum-frequency generation inside an optical cavity has been predicted to be a potential source of three-mode continuous-variable entanglement. When the cavity is pumped by two fields, the threshold properties have been analysed, showing that these are more complicated than in well-known processes such as optical parametric oscillation. When there is only a single pumping field, the entanglement properties have been calculated using a linearised fluctuation analysis, but without any consideration of the threshold properties or critical operating points of the system. In this work we extend this analysis to demonstrate that the singly pumped system demonstrates a rich range of threshold behaviour when quantisation of the pump field is taken into account and that asymmetric polychromatic entanglement is available over a wide range of operational parameters.Comment: 24 pages, 15 figure

On the Nature of the EIS Candidate Clusters: Confirmation of z<0.6 candidates

We use public V-band imaging data from the wide-angle surveys conducted by the ESO Imaging Survey project (EIS) to further investigate the nature of the EIS galaxy cluster candidates. These were originally identified by applying a matched-filter algorithm which used positional and photometric data of the galaxy sample extracted from the I-band survey images. In this paper, we apply the same technique to the galaxy sample extracted from V-band data and compare the new cluster detections with the original ones. We find that ~75% of the low-redshift cluster candidates (z<0.6) are detected in both passbands and their estimated redshifts show good agreement with the scatter in the redshift differences being consistent with the estimated errors of the method. For the ``robust'' I-band detections the matching frequency approaches ~85%. We also use the available (V-I) color to search for the red sequence of early-type galaxies observed in rich clusters over a broad range of redshifts. This is done by searching for a simultaneous overdensity in the three-dimensional color-projected distance space. We find significant overdensities for ~75% of the ``robust'' candidates with z_I<0.6. We find good agreement between the characteristic color associated to the detected "red sequence" and that predicted by passive evolution galaxy models for ellipticals at the redshift estimated by the matched-filter. The results presented in this paper show the usefulness of color data, even of two-band data, to both tentatively confirm cluster candidates and to select possible cluster members for spectroscopic observations. Based on the present results, we estimate that ~150 EIS clusters with z_I<0.6 are real, making it one of the largest samples of galaxy clusters in this redshift range currently available in the southern hemisphere.Comment: 11 pages, 10 figures, accepted for publication in A&

Spectroscopic confirmation of clusters from the ESO imaging survey

We measure redshifts for 67 galaxies in the field of six cluster candidates from the ESO Imaging Survey (EIS). The cluster candidates are selected in the EIS patches C and D among those with estimated mean redshifts between 0.5 and 0.7. The observations were made with EFOSC2 at the 3.6m ESO telescope. In the six candidate cluster fields, we identify 19 possible sets of 2 to 7 galaxies in redshift space. In order to establish which of the 19 sets are likely to correspond to real dense systems we compare our counts with those expected from a uniform distribution of galaxies with given luminosity function. In order to take into account the effect of the Large Scale Structure, we modulate the probability computed from the luminosity function with random samplings of the Canada-France Redshift Survey. We find that four out of six candidate EIS clusters are likely to correspond to real systems in redshift space (> 95 % confidence level). Two of these systems have mean redshift in agreement with the redshift estimate given by the matched filter algorithm. The other two systems have significantly lower redshifts. We discuss the implications of our results in the context of our ongoing research projects aimed at defining high-redshift optically-selected cluster samples.Comment: To appear in A&A, main journal -- 12 pages, 9 figure

Inorganic carbon and water masses in the Irminger Sea since 1991

The subpolar region in the North Atlantic is a major sink for anthropogenic carbon. While the storage rates show large interannual variability related to atmospheric forcing, less is known about variability in the natural dissolved inorganic carbon (DIC) and the combined impact of variations in the two components on the total DIC inventories. Here, data from 15 cruises in the Irminger Sea covering the 24-year period between 1991 and 2015 were used to determine changes in total DIC and its natural and anthropogenic components. Based on the results of an extended optimum multiparameter analysis (eOMP), the inventory changes are discussed in relation to the distribution and evolution of the main water masses. The inventory of DIC increased by 1.43±0.17 molm-2 yr-1 over the period, mainly driven by the increase in anthropogenic carbon (1.84±0.16 molm-2 yr-1) but partially offset by a loss of natural DIC (-0.5±0.22 molm-2 yr-1). Changes in the carbon storage rate can be driven by concentration changes in the water column, for example due to the ageing of water masses, or by changes in the distribution of water masses with different concentrations either by local formation or advection. A decomposition of the trends into their main drivers showed that variations in natural DIC inventories are mainly driven by changes in the layer thickness of the main water masses, while anthropogenic carbon is most affected by concentration changes. The storage rates of anthropogenic carbon are sensitive to data selection, while changes in DIC inventory show a robust signal on short timescales associated with the strength of convection