El interés emergente por la narrativa como método en el ámbito socio-educativo : el caso de las historias de vida

Los últimos años hemos experimentado un desarrollo importante en el debate sobre la metodología narrativa en la investigación social y educativa. Este artículo comienza explicando la aparición de lo que hoy entendemos como la perspectiva biográfica que evoluciona tarde y despacio; y la expansión de técnicas narrativas. Del mismo modo, trataremos de explicar la variedad de formas y propuestas que existen en la recogida de datos.The past few years have seen the development of an important debate over narrative methodology in social and educational research. This paper begins by explaning the emergence of what now can be termed the biographical pespective follows this latter, quieter, pattern; and the broad expanse of the techniques narratives methods. At the same time we will try to explain the variety of forms and purposes in Narrative methods as collecting dat

El interés emergente por la narrativa como método en el ámbito socio-educativo. El caso de las historias de vida

Los últimos años hemos experimentado un desarrollo importante en el debate sobre la metodología narrativa en la investigación social y educativa. Este artículo comienza explicando la aparición de lo que hoy entendemos como la perspectiva biográfica que evoluciona tarde y despacio; y la expansión de técnicas narrativas. Del mismo modo, trataremos de explicar la variedad de formas y propuestas que existen en la recogida de datos.The past few years have seen the development of an important debate over narrative methodology in social and educational research. This paper begins by explaning the emergence of what now can be termed the biographical pespective follows this latter, quieter, pattern; and the broad expanse of the techniques narratives methods. At the same time we will try to explain the variety of forms and purposes in Narrative methods as collecting data

ERK5 Is a Major Determinant of Chemical Sarcomagenesis: Implications in Human Pathology

Sarcomas are a heterogeneous group of tumors in which the role of ERK5 is poorly studied. To clarify the role of this MAPK in sarcomatous pathology, we used a murine 3-methyl-cholanthrene (3MC)-induced sarcoma model. Our data show that 3MC induces pleomorphic sarcomas with muscle differentiation, showing an increased expression of ERK5. Indeed, this upregulation was also observed in human sarcomas of muscular origin, such as leiomyosarcoma or rhabdomyosarcoma. Moreover, in cell lines derived from these 3MC-induced tumors, abrogation of Mapk7 expression by using specific shRNAs decreased in vitro growth and colony-forming capacity and led to a marked loss of tumor growth in vivo. In fact, transcriptomic profiling in ERK5 abrogated cell lines by RNAseq showed a deregulated gene expression pattern for key biological processes such as angiogenesis, migration, motility, etc., correlating with a better prognostic in human pathology. Finally, among the various differentially expressed genes, Klf2 is a key mediator of the biological effects of ERK5 as indicated by its specific interference, demonstrating that the ERK5–KLF2 axis is an important determinant of sarcoma biology that should be further studied in human pathology.This work has been supported with Grant RTI2018-094093-B-I00 funded by MCIN/AEI/10.13039/501100011033, “ERDF A way of making Europe” to RSP. Also supported with funds from Fundación Leticia Castillejo Castillo, Roche España and ACEPAIN to RSP and MJRH. RSP and MJRH’s Research Institute and the work carried out in their laboratory, received partial support from the European Community through the FEDER. JJ and EAL hold a predoctoral research contract cofounded by the European Social Fund and UCLM. OR holds a contract for accessing the Spanish System of Science, Technology, and Innovation (SECTI) funded by the University of Castilla-La Mancha (UCLM) and received partial support from the European Social Fund (FSE) through its Operative Program for Castilla-La Mancha (2007–2013

La Mola d'Agres (Alacant): aproximació a la indústria lítica

Desde su descubrimiento, las excavaciones sistemáticas han colocado la Mola d"Agres (Alicante) como yacimiento referencia en el estudio de la Edad del Bronce en tierras valencianas. La finalidad del presente trabajo es realizar una aproximación a la industria lítica tallada a partir de los materiales recuperados en las últimas campañas (2000-2007), así como valorar este conjunto dentro del contexto de la Edad del Bronce

La Mola d’Agres (Alacant): aproximació a la indústria lítica

Des del seu descobriment, les excavacions sistemàtiques han situat la Mola d’Agres (Alacant) com un jaciment de referència en l’estudi de l’Edat del Bronze en terres valencianes. La finalitat del present treball és fer una aproximació a la indústria lítica tallada a partir del material recuperat en les darreres campanyes (2000-2007), així com valorar aquest conjunt dins del context de l’Edat del Bronze. Paraules claus: Edat del Bronze. Alacant. Indústria Lítica. Tipologia.Since the date of its discovery, the systematic excavations have set the Mola d’Agres (Alicante) as a reference archaeological site for the study of the Bronze Age in the Valencian lands. The purpose of this article is to make an approach to the carved lithic industry by means of the materials obtained in the last working campaigns (2000-2007), our aim being as well the valuation and appraisal of this set of materials within the context of the Bronze Age. Key words: Bronze Age. Alicante. Lithic industry. Typology.Desde su descubrimiento, las excavaciones sistemáticas han colocado la Mola d’Agres (Alicante) como yacimiento referencia en el estudio de la Edad del Bronce en tierras valencianas. La finalidad del presente trabajo es realizar una aproximación a la industria lítica tallada a partir de los materiales recuperados en las últimas campañas (2000-2007), así como valorar este conjunto dentro del contexto de la Edad del Bronce. Palabras claves: Edad de Bronce. Alicante. Industria lítica. Tipología

Design of strategies to study the metabolic profile of highly polar compounds in plasma by reversed-phase liquid chromatography-high resolution mass spectrometry

Amino acids and related compounds are paramount analytes which are involved in numerous metabolic pathways. Most of these compounds are unable to be retained on Liquid Chromatography with Reversed Phase stationary phases due to their high hydrophilic character. An interesting strategy is to reduce their polarity through their derivatization with a labelling reagent, such as the commercially available 9-fluorenylmethyloxycarbonyl (FMOC) which forms stable complexes with primary and secondary amine moieties rapidly. Although some derivatization reagents have been employed in the study of metabolic profiles, as far as we know, FMOC has never been employed for this purpose. In this work, it is demonstrated that the use of RP-LCMS(TOF) using a C18 column and FMOC as labelling agent enables the determination of a larger number of hydrophilic compounds (proteinogenic amino acids, non-proteinogenic amino acids, and biogenic amines) when compared to the use of a fully -wettable pentafluorophenyl column in fully-aqueous conditions (gradient starting in 0% of organic solvent) and HILIC column, both without using compound derivatization. Different strategies for plasma protein elimination were also carefully evaluated. Results revealed that ultrafiltration (UF) offered a lower variability from sample to sample when compared to the protein precipitation (PP) method (from 2 to 12 times lower variability found in UF). Additionally, UF preserved a larger number of possible compounds when compared to the PP approach: 4631 unique molecular features with UF, 666 unique molecular features with PP. (C) 2017 Elsevier B.V. All rights reserved

Elección del modo de entrada y resultado de la estrategia internacional de las cadenas hoteleras, bajo el enfoque de opciones reales

Este trabajo presenta un modelo explicativo de la elección del modo de entrada a un mercado internacional y de la repercursión de dicha elección sobre el resultado de la estrategia internacional de las cadenas hoteleras, bajo el enfoque dinámico de la teoría de opciones reales. Para ello se analiza la aplicabilidad de dicha teoría a este campo de estudio, complementándola con las aportaciones de la teoría de los costes de transacción y la teoría del proceso de internacionalización.This paper presents an explanatory model for the entry mode choice to an international market and its implications on the performance of the international strategy in hotel chains, under the dynamic real options theory approach. We examine the applicability of this theory in this field of study, supplemented by contributions from transaction costs and internationalization process theories

Deciphering Master Gene Regulators and Associated Networks of Human Mesenchymal Stromal Cells.

Mesenchymal Stromal Cells (MSC) are multipotent cells characterized by self-renewal, multilineage differentiation, and immunomodulatory properties. To obtain a gene regulatory profile of human MSCs, we generated a compendium of more than two hundred cell samples with genome-wide expression data, including a homogeneous set of 93 samples of five related primary cell types: bone marrow mesenchymal stem cells (BM-MSC), hematopoietic stem cells (HSC), lymphocytes (LYM), fibroblasts (FIB), and osteoblasts (OSTB). All these samples were integrated to generate a regulatory gene network using the algorithm ARACNe (Algorithm for the Reconstruction of Accurate Cellular Networks; based on mutual information), that finds regulons (groups of target genes regulated by transcription factors) and regulators (i.e., transcription factors, TFs). Furtherly, the algorithm VIPER (Algorithm for Virtual Inference of Protein-activity by Enriched Regulon analysis) was used to inference protein activity and to identify the most significant TF regulators, which control the expression profile of the studied cells. Applying these algorithms, a footprint of candidate master regulators of BM-MSCs was defined, including the genes EPAS1, NFE2L1, SNAI2, STAB2, TEAD1, and TULP3, that presented consistent upregulation and hypomethylation in BM-MSCs. These TFs regulate the activation of the genes in the bone marrow MSC lineage and are involved in development, morphogenesis, cell differentiation, regulation of cell adhesion, and cell structure