Using Six Sigma to analyse Customer Satisfaction at the product design and development stage

The need for the products to be first manufactured according to the specifications is becoming a reality due to a situation that exists in the current markets. This is achieved by applying the six-sigma tool. This study is done in a multinational company that manufactures auto parts and aims to analyze the Key Performance Indicator (KPI) Customer Satisfaction (CS) in the design and development stages of the product to always obtain the optimum result, 100%. From the KPI CS data the sigma was calculated using the Define, Measure, Analyze, Improve, Design and Verify (DMADV) methodology. Using the range of values that CS can have to achieve the desired result, the six-sigma tool was applied. From the six-sigma tool, the value of the standard deviation for the results obtained is within the expected. Maximum limits were imposed to work comfortably within the six-sigma. If there is an alert for non-compliance with these limits, corrective and preventive measures can be taken so that there is no recurrence of the problem. When a control limit has been imposed and, even if corrective and preventive actions have been taken, the six sigma parameters are still working. Finally, using the CS calculation formula, the desired Quality Control and Time To Market results were obtained for the range of values within the six sigma with the appropriate control limits. With this study, the company was able to identify where the recurrence of the problems is happening. Knowing the cause of the problems it allows changes or improvements in the processes.info:eu-repo/semantics/publishedVersio

Screening polymeric ionic liquids for chromatography-based purification of bacteriophage M13

M13 bacteriophage is a key instrument in phage display applications, as well as a possible antibacterial therapeutic agent due to its highly restrictive bacterial pathogenesis, and other applications. The traditional phage purification process is usually achieved by gradient ultracentrifugation or a combination of precipitation, centrifugation and microfiltration. These approaches easily lead to long process times, high operational costs, phage aggregation and consequent product loss (approximately 60%). This work is thus focused on an alternative potential large-scale process to achieve high yield and purity while minimizing the operational costs. Electrostatic-based separation processes are also common biomolecules purification techniques. Although anion exchange chromatography has been used before to purify several viral particles, this technique has been poorly reported for the purification of M13 phage. In a recent work, our group has demonstrated the use of a predominant anion exchange process, where a polymeric ionic liquid (PIL) was used as an alternative separation matrix for M13 bacteriophage. In this work, a variety of system parameters was studied, including chemical structure of the cation and the anion, the crosslinker nature and its concentration, either in batch adsorption/elution or chromatographic operation mode. The PIL-based chromatographic operation mode revealed to be a suitable separation process for M13 from directly filtered E. coli supernatant, reaching over 70% M13 recovery and 4.6 purification factor in a single step. To our knowledge, this is the first time that PILs have been reported as separation agents for bioproducts from complex mixtures.publishe

Multitask ATPases (NBDs) of bacterial ABC importers type I and their interspecies exchangeability

We would like to thank Herminia de Lencastre (ITQB-AX NOVA), Adriano Henriques (ITQB-AX NOVA) and PaulaTamagnini (i3S) for the gift of chromosomal DNA, Paulo Tavares (I2BC-CNRS) and Adriano Henriques for the gift of flagellin and anti-sigmaA antibodies, respectively, and Diogo Pereira for carrying out some growth kinetic assays. This work was supported by the FundacAo para a Ciencia e a Tecnologia [Grant Numbers PTDC/BIA-MIC/30696/2017 to ISN and UID/Multi/04378/2019 to Unidade de Ciencias Biomoleculares Aplicadas UCIBIO; and fellowship PD/BD/105737/2014 to F.L.].ATP-binding cassette (ABC) type I importers are widespread in bacteria and play a crucial role in its survival and pathogenesis. They share the same modular architecture comprising two intracellular nucleotide-binding domains (NBDs), two transmembrane domains (TMDs) and a substrate-binding protein. The NBDs bind and hydrolyze ATP, thereby generating conformational changes that are coupled to the TMDs and lead to substrate translocation. A group of multitask NBDs that are able to serve as the cellular motor for multiple sugar importers was recently discovered. To understand why some ABC importers share energy-coupling components, we used the MsmX ATPase from Bacillus subtilis as a model for biological and structural studies. Here we report the first examples of functional hybrid interspecies ABC type I importers in which the NBDs could be exchanged. Furthermore, the first crystal structure of an assigned multitask NBD provides a framework to understand the molecular basis of the broader specificity of interaction with the TMDs.publishersversionpublishe

Effects of in vitro digestion and in vitro colonic fermentation on stability and functional properties of yerba mate (Ilex paraguariensis A. St. Hil.) beverages

Yerba mate (Ilex paraguariensis) is a plant that grows naturally in South America. From its leaves and thin stems different kinds of beverages are prepared (chimarrão, tererê and tea mate), all of them rich in bioactive substances. The aim of this study was to evaluate the influence of in vitro gastrointestinal digestion and colonic fermentation on the stability of the polyphenols and on the antioxidant, antimicrobial and antitumoral activities of the yerba mate beverages. The phenolic chromatographic profile revealed that both the in vitro digestion and the colonic fermentation caused a pronounced decrease in 3,5-O-dicaffeoylquinic acid and 5-O-caffeoylquinic acid in the preparations. However, 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid and salvianolic acid I were only barely affected in all preparations. Despite the decrease in the phytochemicals content, yerba mate beverages maintain their functional properties such as antioxidant, antibacterial and antitumoral activities.The authors thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Proc. 3079/2015-8) and Fundação Araucária (Proc.24/2012) for funding this study. Authors V.G. Correa and G.A. Gonçalves thanks Coordenação de Aperfeiçoamento do Pessoal do Ensino Superior (CAPES) for the financial support provided for their post-graduate studies in Universidade Estadual de Maringá. A. Bracht, and R.M. Peralta research grant recipients of CNPq. The authors are also thankful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Program PT2020 for financial support to CIMO (UID/AGR/00690/2013), L. Barros (SFRH/BPD/107855/2015) and M.I. Dias (SFRH/BD/84485/2012) grant. To POCI-01-0145-FEDER-006984 (LA LSRE-LCM), funded by ERDF, through POCI-COMPETE2020 and FCT.info:eu-repo/semantics/publishedVersio

Projeto de Intervenção para Melhoria do Desempenho Cognitivo em Doentes com Défice Cognitivo Ligeiro

INTRODUÇÃO: Em 2018, um estudo de investigação realizado numa Unidade de Saúde Familiar (USF) estimou uma prevalência de défice cognitivo ligeiro (DCL) de 29,1%, verificando-se uma associação entre o melhor desempenho no teste Montreal Cognitive Assessement (MoCA) com a leitura de jornais e livros e a realização de passatempos. O objetivo do projeto foi melhorar a capacidade cognitiva nos idosos com DCL através de atividades de treino cognitivo. MÉTODOS: Projeto de intervenção realizado numa USF com uma amostra de 20 idosos com DCL. Decorreram sessões presenciais de treino cognitivo e foram entregues cadernos de trabalhos de casa. Foi aplicado o MoCA versão portuguesa aos zero, três e seis meses para avaliação do desempenho cognitivo. RESULTADOS: No início do estudo, a pontuação mediana no MoCA foi 18,5. Após seis meses, registou-se uma pontuação mediana de 23. Verificou-se uma melhoria da pontuação global em todos os domínios cognitivos, com exceção da linguagem e da orientação. CONCLUSÃO: O objetivo foi atingido pela melhoria do desempenho cognitivo após seis meses de estudo. O treino cognitivo tem um potencial efeito benéfico na saúde da população idosa e requer materiais de simples elaboração e baixo custo que podem ser aplicados em lares de idosos e centros de dia através dos cuidadores

Projeto de Intervenção para Melhoria do Desempenho Cognitivo em Doentes com Défice Cognitivo Ligeiro

INTRODUÇÃO: Em 2018, um estudo de investigação realizado numa Unidade de Saúde Familiar (USF) estimou uma prevalência de défice cognitivo ligeiro (DCL) de 29,1%, verificando-se uma associação entre o melhor desempenho no teste Montreal Cognitive Assessement (MoCA) com a leitura de jornais e livros e a realização de passatempos. O objetivo do projeto foi melhorar a capacidade cognitiva nos idosos com DCL através de atividades de treino cognitivo. MÉTODOS: Projeto de intervenção realizado numa USF com uma amostra de 20 idosos com DCL. Decorreram sessões presenciais de treino cognitivo e foram entregues cadernos de trabalhos de casa. Foi aplicado o MoCA versão portuguesa aos zero, três e seis meses para avaliação do desempenho cognitivo. RESULTADOS: No início do estudo, a pontuação mediana no MoCA foi 18,5. Após seis meses, registou-se uma pontuação mediana de 23. Verificou-se uma melhoria da pontuação global em todos os domínios cognitivos, com exceção da linguagem e da orientação. CONCLUSÃO: O objetivo foi atingido pela melhoria do desempenho cognitivo após seis meses de estudo. O treino cognitivo tem um potencial efeito benéfico na saúde da população idosa e requer materiais de simples elaboração e baixo custo que podem ser aplicados em lares de idosos e centros de dia através dos cuidadores

Anti-inflammatory and antioxidant nanostructured cellulose membranes loaded with phenolic-based ionic liquids for cutaneous application

The utilization of natural compounds, such as phenolic acids and biopolymers, in the healthcare domain is gaining increasing attention. In this study, bacterial nanocellulose (BC) membranes were loaded with ionic liquids (ILs) based on phenolic acids. These ionic compounds, with improved solubility and bioavailability, were prepared by combining the cholinium cation with anions derived from caffeic, ellagic and gallic acids. The obtained BC-ILs membranes were homogeneous, conformable and their swelling ability agreed with the solubility of each IL. These membranes revealed a controlled ILs dissolution rate in the wet state and high antioxidant activity. In vitro assays performed with Raw 264.7 macrophages and HaCaT keratinocytes revealed that these novel BC-ILs membranes are non-cytotoxic and present relevant anti-inflammatory properties. Diffusion studies with Hanson vertical diffusion cells showed a prolonged release profile of the ILs from the BC membranes. Thus, this work, successfully demonstrates the potential of BC-ILs membranes for skin treatment.publishe

Comparison of the neuropsychological assessment in a girl with bilateral cerebrovascular disease (moyamoya) before and after surgical intervention

Moyamoya is a chronic progressive cerebrovascular disease with characteristic angiographic findings and a clinical picture with episodes of transient ischemic attacks, headache, seizures, hemiparesis, which may resolve after surgical treatment. We describe the case of a girl with the typical findings of the disease, comparing them before and after surgery with the use of neuropsychological tests, neurological examination and laboratory tests.A doença de moyamoya é anormalidade cerebrovascular crônica e progressiva identificada através das características angiográficas; estão presentes no quadro clínico episódios isquêmicos transitórios, cefaléia, crises convulsivas, hemiparesia, que podem desaparecer após tratamento cirúrgico. Nós descrevemos o caso de uma menina com características clássicas da doença, comparando-o em dois momentos, antes e depois da cirurgia, através de avaliações neurológicas, neuropsicológicas, e exames complementares.1036104

A comprehensive assessment of the transcriptome of cork oak (Quercus suber) through EST sequencing

Background: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic information. Cork oak forests are of great ecological importance and represent a major economic and social resource in Southern Europe and Northern Africa. However, global warming is threatening the cork oak forests by imposing thermal, hydric and many types of novel biotic stresses. Despite the economic and social value of the Q. suber species, few genomic resources have been developed, useful for biotechnological applications and improved forest management. Results: We generated in excess of 7 million sequence reads, by pyrosequencing 21 normalized cDNA libraries derived from multiple Q. suber tissues and organs, developmental stages and physiological conditions. We deployed a stringent sequence processing and assembly pipeline that resulted in the identification of ~159,000 unigenes. These were annotated according to their similarity to known plant genes, to known Interpro domains, GO classes and E.C. numbers. The phylogenetic extent of this ESTs set was investigated, and we found that cork oak revealed a significant new gene space that is not covered by other model species or EST sequencing projects. The raw data, as well as the full annotated assembly, are now available to the community in a dedicated web portal at http://www.corkoakdb.org. Conclusions: This genomic resource represents the first trancriptome study in a cork producing species. It can be explored to develop new tools and approaches to understand stress responses and developmental processes in forest trees, as well as the molecular cascades underlying cork differentiation and disease response.Peer Reviewe