Pancreatic exocrine insufficiency after bariatric surgery

Morbid obesity is a lifelong disease, and all patients require complementary follow-up including nutritional surveillance by a multidisciplinary team after bariatric procedures. Pancreatic exocrine insufficiency (PEI) refers to an insufficient secretion of pancreatic enzymes and/or sodium bicarbonate. PEI is a known multifactorial complication after upper gastrointestinal surgery, and might constitute an important clinical problem due to the large number of bariatric surgical procedures in the world. Symptoms of PEI often overlap with sequelae of gastric bypass, making the diagnosis difficult. Steatorrhea, weight loss, maldigestion and malabsorption are pathognomonic for both clinical conditions. Altered anatomy after bypass surgery can make the diagnostic process even more difficult. Fecal elastase-1 (FE1) is a useful diagnostic test. PEI should be considered in all patients after bariatric surgery with prolonged gastrointestinal complaints that are suggestive of maldigestion and/or malabsorption. Appropriate pancreatic enzyme replacement therapy should be part of the treatment algorithm in patients with confirmed PEI or symptoms suggestive of this complication

Immunological considerations of modern animal models of malignant primary brain tumors

Recent advances in animal models of glioma have facilitated a better understanding of biological mechanisms underlying gliomagenesis and glioma progression. The limitations of existing therapy, including surgery, chemotherapy, and radiotherapy, have prompted numerous investigators to search for new therapeutic approaches to improve quantity and quality of survival from these aggressive lesions. One of these approaches involves triggering a tumor specific immune response. However, a difficulty in this approach is the the scarcity of animal models of primary CNS neoplasms which faithfully recapitulate these tumors and their interaction with the host's immune system. In this article, we review the existing methods utilized to date for modeling gliomas in rodents, with a focus on the known as well as potential immunological aspects of these models. As this review demonstrates, many of these models have inherent immune system limitations, and the impact of these limitations on studies on the influence of pre-clinical therapeutics testing warrants further attention

The role of quenching time in the evolution of the mass-size relation of passive galaxies from the WISP survey

We analyze how passive galaxies at z $\sim$ 1.5 populate the mass-size plane as a function of their stellar age, to understand if the observed size growth with time can be explained with the appearance of larger quenched galaxies at lower redshift. We use a sample of 32 passive galaxies extracted from the Wide Field Camera 3 Infrared Spectroscopic Parallel (WISP) survey with spectroscopic redshift 1.3 $\lesssim$ z $\lesssim$ 2.05, specific star-formation rates lower than 0.01 Gyr$^{-1}$, and stellar masses above 4.5 $\times$ 10$^{10}$ M$_\odot$. All galaxies have spectrally determined stellar ages from fitting of their rest-frame optical spectra and photometry with stellar population models. When dividing our sample into young (age $\leq$ 2.1 Gyr) and old (age $>$ 2.1 Gyr) galaxies we do not find a significant trend in the distributions of the difference between the observed radius and the one predicted by the mass-size relation. This result indicates that the relation between the galaxy age and its distance from the mass-size relation, if it exists, is rather shallow, with a slope alpha $\gtrsim$ -0.6. At face value, this finding suggests that multiple dry and/or wet minor mergers, rather than the appearance of newly quenched galaxies, are mainly responsible for the observed time evolution of the mass-size relation in passive galaxies.Comment: Accepted for publication in ApJ Letters; 6 pages, 3 figures, 1 tabl

A link between the ice nucleation activity and the biogeochemistry of seawater

Emissions of ice-nucleating particles (INPs) from sea spray can impact climate and precipitation by changing cloud formation, precipitation, and albedo. However, the relationship between seawater biogeochemistry and the ice nucleation activity of sea spray aerosols remains unclarified. Here, we demonstrate a link between the biological productivity in seawater and the ice nucleation activity of sea spray aerosol under conditions relevant to cirrus and mixed-phase cloud formation. We show for the first time that aerosol particles generated from both subsurface and microlayer seawater from the highly productive eastern tropical North Pacific Ocean are effective INPs in the deposition and immersion freezing modes. Seawater particles of composition similar to subsurface waters of highly productive regions may therefore be an unrealized source of effective INPs. In contrast, the subsurface water from the less productive Florida Straits produced less effective immersion mode INPs and ineffective depositional mode INPs. These results indicate that the regional biogeochemistry of seawater can strongly affect the ice nucleation activity of sea spray aerosol

Surgery of primary melanomas

Surgery remains the mainstay of melanoma therapy, regardless of the tumor site. Only the early diagnosis combined with proper surgical therapy currently gives patients affected by this malignancy the chance for a full cure. The main goal of surgical therapy is to provide the local control of the disease and to secure long-term survival of the patient without reasonable functional and esthetic impairment. The recommended method of biopsy-excisional biopsy, as an initial diagnostic and, to some extent, therapeutic procedure-is performed under local anesthesia as an elliptical incision with visual clear margins of 1-3 mm and with some mm of subcutaneous tissue. The extent of radical excision of the primary tumor (or scar after excisional biopsy) is based on the histopathologic characteristics of the primary tumor and usually consists of 1-2 cm margins with primary closure. The philosophy behind conducted randomized clinical trials has been to find the most conservative surgical approach that is able to guarantee the same results as more demolitive treatment. This has been the background of the trials designed to define the correct margins of excision around a primary cutaneous melanoma. Much less definition can be dedicated to the surgical management of patients with non-cutaneous melanomas

HST-WFC3 Near-Infrared Spectroscopy of Quenched Galaxies at zeta approx 1.5 from the WISP Survey: Stellar Populations Properties

We combine Hubble Space Telescope (HST) G102 and G141 near-IR (NIR) grism spectroscopy with HST/WFC3- UVIS, HST/WFC3-IR, and Spitzer/IRAC [3.6 microns] photometry to assemble a sample of massive (log(Mstar/M solar mass) at approx 11.0) and quenched (specific star formation rate 2 and the zeta approx 1.5 RS. According to their estimated ages, the time required for quenched galaxies off the RS to join their counterparts on the z approx. 1.5 RS is of the order of approx. 1G/yr

Newborn spheroids at high redshift: when and how did the dominant, old stars in today's massive galaxies form?

We study ~330 massive (M* > 10^9.5 MSun), newborn spheroidal galaxies (SGs) around the epoch of peak star formation (1<z<3), to explore the high-redshift origin of SGs and gain insight into when and how the old stellar populations that dominate today's Universe formed. The sample is drawn from the HST/WFC3 Early-Release Science programme, which provides deep 10-filter (0.2 - 1.7 micron) HST imaging over a third of the GOODS-South field. We find that the star formation episodes that built the SGs likely peaked in the redshift range 2<z<5 (with a median of z~3) and have decay timescales shorter than ~1.5 Gyr. Starburst timescales and ages show no trend with stellar mass in the range 10^9.5 < M* < 10^10.5 MSun. However, the timescales show increased scatter towards lower values ( 10^10.5 MSun, and an age trend becomes evident in this mass regime: SGs with M* > 10^11.5 MSun are ~2 Gyrs older than their counterparts with M* < 10^10.5 MSun. Nevertheless, a smooth downsizing trend with galaxy mass is not observed, and the large scatter in starburst ages indicate that SGs are not a particularly coeval population. Around half of the blue SGs appear not to drive their star formation via major mergers, and those that have experienced a recent major merger, show only modest enhancements (~40%) in their specific star formation rates. Our empirical study indicates that processes other than major mergers (e.g. violent disk instability driven by cold streams and/or minor mergers) likely play a dominant role in building SGs, and creating a significant fraction of the old stellar populations that dominate today's Universe.Comment: MNRAS in pres

Endoplasmic reticulum stress in amelogenesis imperfecta and phenotypic rescue using 4-phenylbutyrate

Inherited diseases caused by geneticmutations can arise due to loss of protein function. Alternatively, mutated proteins may mis-fold, impairing endoplasmic reticulum (ER) trafficking, causing ER stress and triggering the unfolded protein response (UPR). The UPR attempts to restore proteostasis but if unsuccessful drives affected cells towards apoptosis. Previously, we reported that in mice, the p. Tyr64Hismutation in the enamel extracellular matrix (EEM) protein amelogenin disrupts the secretory pathway in the enamel-forming ameloblasts, resulting in eruption of malformed tooth enamel that phenocopies human amelogenesis imperfecta (AI). Defective amelogenin post-secretory self-assembly and processing within the developing EEM has been suggested to underlie the pathogenesis of Xchromosome-linked AI. Here, wechallenge thisconceptbyshowing that AI pathogenesis associated with the p. Tyr64His amelogenin mutation involves ameloblast apoptosis induced by ER stress. Furthermore, we show that 4-phenylbutyrate can rescue the enamel phenotype in affected female mice by promoting cell survival over apoptosis such that they are able tocomplete enamel formation despite the presence of the mutation, offering a potential therapeutic option for patients with this form of AI and emphasizing the importance of ER stress in the pathogenesis of this inherited conformational disease

A phase II study of a human anti-PDGFR alpha monoclonal antibody (olaratumab, IMC-3G3) in previously treated patients with metastatic gastrointestinal stromal tumors

Experimentele farmacotherapi