Laboratorio virtual para autoaprendizaje en ingeniería.Taquimetría en TOPLAB, LV de observaciones topográficas UPM

Ante el potencial de los laboratorios virtuales, LV, en la enseñanza en ingeniería y para su explotación en abierto desde el portal de laboratorios virtuales de la UPM, el GIE INNGEO de la ETSITGC ha desarrollado un módulo fundamental para la Taquimetría, con 5 prácticas con estación total, que se suma al módulo inicial de nivelación geométrica de TOPLAB (LV de observaciones topográficas UPM). Supone para las asignaturas de Topografía y Geomática de cualquier ingeniería un recurso didáctico muy apreciado por profesores y alumnos, con el potencial pedagógico y ventajas de los LV (en particular para las observaciones topográficas, dado el alto coste del instrumental y la dependencia de condiciones atmosféricas favorables para poder observar al aire libre). Además, como valor añadido, se han incorporado dos sistemas: un “Tutor virtual” que guía y supervisa la ejecución de cada práctica y un, denominado, “Registro de alumnos” que, para cada usuario, verifica su observación y cálculo proporcionando una retroalimentación inmediata y, para los profesores o gestores, facilita el análisis y evaluación del trabajo realizado. por el alumno en el LV, a través de un registro en web creado expresamente para TOPLAB

Are Peripheral Biomarkers Determinants of Eating Styles in Childhood and Adolescence Obesity? A Cross-Sectional Study

Disturbances in eating behaviors have been widely related to obesity. However, little is known about the role of obesity-related biomarkers in shaping habitual patterns of eating be haviors (i.e., eating styles) in childhood. The objective of the present study was to explore the relationships between several biomarkers crucially involved in obesity (ghrelin, insulin resistance, and leptin/adiponectin ratio) and eating styles in children and adolescents with obesity. Seventy participants aged between 8 and 16 (56.2% men) fulfilled the Spanish version of the Dutch Eating Behavior Questionnaire for Children to measure external, emotional, and restrained eating styles. In addition, concentrations of ghrelin, leptin, adiponectin, insulin, and glucose were obtained through a blood test. Hierarchical multiple regression analyses controlling for age and sex were computed for each eating style. Results indicated that individuals with higher ghrelin concentration levels showed lower scores in restrained eating (β = −0.61, p < 0.001). The total model explained 32% of the variance of the restrained pattern. No other relationships between obesity-related biomarkers and eating behaviors were found. This study highlights that one of the obesity-risk factors, namely lower plasma ghrelin levels, is substantially involved in a well-known maladaptive eating style, restraint eating, in childhood obesity

Direct and label-free monitoring oligonucleotide immobilization, non-specific binding and DNA biorecognition

NOTICE: this is the author’s version of a work that was accepted for publication in Sensors and Actuators B: Chemical. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Sensors and Actuators B: Chemical, [VOL 192, (1 March 2014)] DOI 10.1016/j.snb.2013.10.110DNA binding chemistry on silicon surface has been investigated. Aminated oligonucleotide probes were immobilized on the chip surface by chemical silanization and further covalent attachment. The chemistries employed were the classical 3-aminopropyltriethoxysilane/glutaraldehyde and, for comparison purposes, the novel 3-isocyanatepropyltriethoxysilane, that allows the direct attachment of the aminated probe. Alternatively, a thiolated oligonucleotide was also photochemically immobilized by means of a thioether linkage. The experiments were followed label-free by Dual Polarization Interferometry. All chemical and photochemical methods gave rise to a probe density immobilization in the order of 1.0-2.5 x 10(10) molecules/mm(2), similar to the values reported for other chemistries. The obtained data suggest that DNA strands are anchored in a different conformation depending on the immobilization method employed. In order to avoid non-specific binding of target molecules, ethanolamine and inert proteins were assayed, and successful results were obtained when using small size proteins such as gelatine. Hybridization efficiency was around 20% for aminosilane-based immobilized probes, and more than 4-fold this value when the other immobilization methods were employed. The ability for recognition complementary DNA strands discriminating non-complementary ones was applied for species identification in mixtures. (C) 2013 Elsevier B.V. All rights reserved.Research projects MASCREEN CTQ2010-15943 from the Spanish Ministerio de Ciencia e Innovacion, and PROMETEO 2010/008 from the Generalitat Valenciana are gratefully acknowledged for financial support.López Paz, JL.; González Martínez, MÁ.; Escorihuela Fuentes, J.; Bañuls Polo, MJ.; Puchades Pla, R.; Maquieira Catala, Á. (2014). Direct and label-free monitoring oligonucleotide immobilization, non-specific binding and DNA biorecognition. Sensors and Actuators B: Chemical. 192:221-228. https://doi.org/10.1016/j.snb.2013.10.110S22122819

Sialyltransferase inhibitor Ac53FaxNeu5Ac reverts the malignant phenotype of pancreatic cancer cells, and reduces tumor volume and favors T-cell infiltrates in mice

Hypersialylation is a feature of pancreatic ductal adenocarcinoma (PDA) and it has been related to tumor malignancy and immune suppression. In this work, we have evaluated the potential of the sialyltransferase inhibitor, Ac53FaxNeu5Ac, to decrease tumor sialoglycans in PDA and to revert its malignant phenotype. Sialoglycans on PDA cells were evaluated by flow cytometry, and the functional impact of Ac53FaxNeu5Ac was assessed using E-selectin adhesion, migration, and invasion assays. PDA tumors were generated in syngeneic mice from KC cells and treated with Ac53FaxNeu5Ac to evaluate tumor growth, mice survival, and its impact on blocking sialic acid (SA) and on the tumor immune component. Ac53FaxNeu5Ac treatment on human PDA cells decreased α2,3-SA and sialyl-Lewisx, which resulted in a reduction in their E-selectin adhesion, and in their migratory and invasive capabilities. Subcutaneous murine tumors treated with Ac53FaxNeu5Ac reduced their volume, their SA expression, and modified their immune component, with an increase in CD8+ T-lymphocytes and NK cells. In conclusion, Ac53FaxNeu5Ac treatment weakened PDA cells' malignant phenotype, thereby reducing tumor growth while favoring anti-tumor immune surveillance. Altogether, these results show the positive impact of reducing SA expression by inhibiting cell sialyltransferases and open the way to use sialyltransferase inhibitors to target this dismal disease.This research was funded by Spanish Ministry of Science and Innovation (grant PID2020-115686RB-I00) and University of Girona (PONT2019/20- and PONT2020/04) to R.P., and MICINN-FIS PI20/00625 to P.N

Proyecto de Biblioteca en una escuela infantil de niños y niñas sordos y oyentes : el Bosque de los Cuentos

La importancia de la lectura, tanto de cuentos como de libros, en edades tempranas para niños sordos y oyentes motiva a este colegio a desarrollar una serie de actividades. Los objetivos de este trabajo son enriquecer, mediante la fusión de comunicación oral y de signos, la inteligencia emocional de los alumnos, padres y profesores; favorecer el hábito de la lectura y la utilización de las bibliotecas; y conocer las características y valores de la cultura de la comunidad sorda y oyente. Se trabaja con cuentos inventados que contemplan realidades familiares y cotidianas de los niños de la escuela, a través de gráficos, plástica y cuenta cuentos. Además del proyecto, se elaboran cuentos de cultura sorda y oyente en papel y otros materiales; marionetas de los distintos personajes; y un catálogo de libros y cuentos adaptados. La evaluación es positiva, hasta el punto de incorporar el taller de biblioteca en el próximo curso para facilitar la implicación de niños y familias, no sólo durante la semana del cuento sino durante todo el curso. Los anexos incluyen los cuentos inventados.Madrid (Comunidad Autónoma). Consejería de Educación. Dirección General de Mejora de la Calidad de la EnseñanzaMadridMadrid (Comunidad Autónoma). Subdirección General de Formación del Profesorado. CRIF Las Acacias; General Ricardos 179 - 28025 Madrid; Tel. + 34915250893ES

Proyecto Verbum

Se desarrolla un proyecto de innovación educativa que pretende incrementar el número de lecturas efectuadas por el alumnado tomando como referencia los años anteriores, alcanzando un grado de disfrute de la propia actividad de leer. Para motivar el hábito de leer se procura vincular los contenidos de los textos a leer a los intereses y preocupaciones del alumnado, proporcionando un listado y son ellos mismos quines elige sus lecturas. Se trata de concienciar ala alumnado de que la lectura y el buen uso de la lengua son instrumentos fundamentales para su desarrollo personal y educativo. El proyecto pretende a su vez implicar a los familiares en el proceso educativo de sus hijos o hijas, abriendo nuevas vías de comunicación entre las personas y propiciando encuentros entre lectores, ya sean presenciales o a través de la red. A través de las Nuevas Tecnologías se pretende ampliar las posibilidades técnicas en las actividades lectoras. El profesorado presenta los argumentos, acciones o personajes de los libros de lectura a elegir para que elija el que prefiera. El alumnado una vez leído el libro elegido, realiza un comentario del texto e invita a sus compañeros y compañeras a leerlo si así lo cree conveniente a modo de consejo. Los alumnos y alumnas que han leído un mismo libro preparan en grupo una exposición ante el resto de la clase, del contenido del libro, de sus impresiones, valoraciones y estructura. El profesorado de las diferentes áreas advierte al alumnado los contenidos relacionados en la lectura con su materia y les proporciona información adicional que les permita sacar el máximo provecho de la lectura. En el centro se han desarrolla actividades extraescolares y complementarias como encuentros con escritores, exposiciones sobre autores y obras, recitales de poesía, lecturas dramatizadas. La valoración general del proyecto tanto por parte del profesorado como del alumnado se es positiva, a pesar de las dificultades generadas.Castilla y LeónConsejería de Educación. Dirección General de Universidades e Investigación; Monasterio de Nuestra Señora de Prado, Autovía Puente Colgante s. n.; 47071 Valladolid; +34983411881; +34983411939ES

Diet as a moderator in the association of sedentary behaviors with inflammatory biomarkers among adolescents in the HELENA study

AIM: To assess if a healthy diet might attenuate the positive sedentary-inflammation relation, whereas an unhealthy diet may increase the effect of sedentary behaviors on inflammatory biomarkers. METHODS: In 618 adolescents (13-17 years) of the European HELENA study, data were available on body composition, a set of inflammation markers, and food intake assessed by a self-administered computerized 24 h dietary recall for 2 days. A 9-point Mediterranean diet score and an antioxidant-rich diet z-score were used as dietary indices and tested as moderators. A set of low-grade inflammatory characteristics was used as outcome: several cytokines in an inflammatory ratio (IL-6, IL-10, TNF-α, TGFβ-1), C-reactive protein, three cell-adhesion molecules (sVCAM-1, sICAM-1, sE-selectin), three cardiovascular risk markers (GGT, ALT, homocysteine) and three immune cell types (white blood cells, lymphocytes, CD3). Sedentary behaviors were self-reported and analyzed as total screen time. Multiple linear regression analyses tested moderation by diet in the sedentary behaviors-inflammation association adjusted for age, sex, country, adiposity (sum of six skinfolds), parental education, and socio-economic status. RESULTS: Both diet scores, Mediterranean and antioxidant-rich diet, were significant protective moderators in the effect of sedentary behaviors on alanine-transaminase enzyme (P = 0.014; P = 0.027), and on the pro/anti-inflammatory cytokine ratio (P = 0.001; P = 0.004), but not on other inflammatory parameters. CONCLUSION: A higher adherence to the Mediterranean diet or an antioxidant-rich diet may attenuate the onset of oxidative stress signs associated by sedentary behaviors, whereas a poor diet seems to increase inflammation