Calidad de vida relacionado a la salud bucal en adultos mayores del Centro de Salud La Esperanza – 2023

Objetivo: Determinar la calidad de vida relacionado a la salud bucal en adultos mayores en Centro de Salud de La Esperanza -Trujillo 2023. Métodos: Tipo de estudio básico, enfoque cuantitativo, de diseño no experimental, nivel descriptivo y de corte transversal, el instrumento utilizado es un cuestionario compuesto por tres dimensiones función física, función psicosocial y función dolor, conformado por 12 ítems, se realizó prueba piloto y mediante prueba alfa de Cronbach obtuvimos el coeficiente de confiabilidad 0.77, el cual da como confiabilidad alta el instrumento. El tipo de muestreo que se uso es el método no probabilístico por conveniencia a 230 adultos mayores que acudan al Centro de Salud de La Esperanza – Trujillo. Se uso el método de análisis descriptivo e inferencial. Resultados: Con respecto a la calidad de vida relacionado a la Salud Bucal de adultos mayores del Centro de Salud de La Esperanza, predominó el nivel bajo con 77,26%, en sexo predomino el nivel bajo, en mujeres con 55,22% y en varones 23,04% y según la edad predomino el nivel bajo. Conclusión: La calidad de vida relacionado a la salud bucal en adultos mayores en Centro de Salud de La Esperanza -Trujillo, fue baja, según sexo fue baja para ambos sexos y según edad fue baja para las distintas edades

How do galaxies populate haloes in high-density environments? An analysis of the Halo Occupation Distribution in future virialized structures

There are hints suggesting that properties of galaxy populations in dark matter haloes may depend on their large-scale environment. Recent works point out that very low-density environments influence halo occupation distribution (HOD), however there is not a similar analysis focused on high-density environments. Here we use a simulated set of future virialized superstructures (FVS) to analyse the occupation of galaxies in haloes within these high globally dense regions. We use a publicly available simulated galaxy set constructed with a semi-analytical model to identify FVS in the simulation. Then, we computed the HOD within these superstructures for different absolute magnitude thresholds and make several analysis including the comparison to the global HOD results. We study the dependence on the results on properties of the FVS such as density and volume as well as consider the morphology of galaxies. We also analysed the properties of the stellar content of galaxies and the formation time of the haloes inside FVS. We find a significant increase in the HOD inside FVS. This result is present for all absolute magnitude thresholds explored. The effect is larger in the densest regions of FVS, but does not depend on the volume of the superstructure. We also find that the stellar-mass content of galaxies considerably differs inside the superstructures. Low mass haloes have their central and satellite galaxies with a higher stellar mass content (50%), and exhibit mean star ages (20%) older than average. For massive haloes in FVS we find that only the stellar mass of satellite galaxies varies considerably corresponding to a decrease of 50%. We find a significant statistical difference between the formation times of haloes in FVS and the average population. Haloes residing in superstructures formed earlier, a fact that leads to several changes in the HOD and their member galaxy properties.Comment: 12 pages, 14 figures, the paper has been accepted to be published by the A&

An electro-mechanical bioreactor providing physiological cardiac stimuli

In cardiac tissue engineering it has been widely demonstrated the fundamental role of physical stimuli in improving structural and functional properties of the engineered cardiac constructs. An electro-mechanical bioreactor has been designed and developed to provide physiological uniaxial stretching and electrical stimuli for inducing functional differentiation and promoting morphological and structural maturation of cultured cardiac constructs obtained from stem cell-seeded scaffolds. The bioreactor is composed of: a transparent and sterilizable culture chamber for housing four cell-seeded scaffolds and the culture medium (working volume = 70 ml); a mechanical stimulation system, with a dedicated grasping system, to provide cyclic stretching (strain up to 20%, cycling frequency up to 2 Hz); an electrical stimulation system to provide electrical monophasic square pulses (1-6 V/cm, 0.25-10 ms, 1-5 Hz); a recirculation system for the automated medium change; a control system for data acquisition and mechanical stimulation. Preliminary in-house tests confirmed the suitability and the performances of the bioreactor as regards fittingness of chamber isolation, grasping system, and physical stimulation systems. Cell culture tests are in progress for investigating the influence of stretching and electrical stimuli on development of engineered cardiac constructs. Due to its high versatility, this bioreactor is a multipurpose adaptable system for dynamic culture of cell-seeded scaffolds for tissue engineering research and applications

FACTORES SOCIODEMOGRÁFICOS Y CLÍNICOS ASOCIADOS A LA TUBERCULOSIS RESISTENTE EN UNA ASEGURADORA DEL VALLE DEL CAUCA 2020

Introduction: In the present investigation, the term resistant tuberculosis was used to refer to all types of resistance to this disease, covering the sociodemographic and clinical factors that are usually related to it.  Objective: To determine the sociodemographic and clinical factors associated with resistant tuberculosis in an insurance company in Valle del Cauca 2020. Methodology: This is a cross-sectional observational epidemiological study with analytical intent, since it aims to determine the sociodemographic and clinical factors that best explain resistant tuberculosis. The study area is event 813 SIVIGILA, which is tuberculosis, specifically cases reported in affiliates of an insurance company in Valle del Cauca during 2020. Results: according to the differential focus populations, it was more prevalent in the prison population, which corresponds to persons on parole or home detention; the municipalities with the highest number of notified and confirmed cases of tuberculosis were Santiago de Cali, Buenaventura, Palmira, Buga and Tuluá. Conclusions: Most of the notified and confirmed tuberculosis cases in 2020, were male, urban area residents, affiliated to the subsidized regime, low socioeconomic strata (1 and 2), Afro-Colombian ethnicity, the prevalence of resistant tuberculosis was 6.9% equivalent to 32 confirmed cases.Introducción: En la presente investigación se utilizó el término tuberculosis resistente para referirse a todos los tipos de resistencia que se presenten frente a esta enfermedad, abarcando los factores sociodemográficos y clínicos que suelen tener relación con la misma.   Objetivo: Determinar los factores sociodemográficos y clínicos asociados a la tuberculosis resistente en una aseguradora en el Valle del Cauca 2020. Metodología: Es un estudio epidemiológico observacional transversal con intención analítica, puesto que pretende determinar los factores sociodemográficos y clínicos que mejor expliquen la tuberculosis resistente. El área de estudio es el evento 813 SIVIGILA, que es la tuberculosis, específicamente casos notificados en afiliados a una aseguradora en el Valle del Cauca durante lo corrido de 2020. Resultados: de acuerdo a las poblaciones de enfoque diferencial fue más prevalente en la población carcelaria, que corresponde a personas en libertad condicional o prisión domiciliaria, los municipios con mayor número de casos notificados y confirmados con tuberculosis fueron Santiago de Cali, Buenaventura, Palmira, Buga y Tuluá. Conclusiones: La mayoría de los casos notificados y confirmados tuberculosis en el 2020, fueron de sexo masculino, residentes en el área urbana, afiliados al régimen subsidiado, de estratos socioeconómicos bajos (1 y 2), de etnia afrocolombiana, la prevalencia de tuberculosis resistente fue de 6,9% equivalente a 32 casos confirmados

Comprehensive management of posterior shoulder instability: diagnosis, indications, and technique for arthroscopic bone block augmentation

Recurrent posterior glenohumeral instability is an entity that demands a high clinical suspicion and a detailed study for a correct approach and treatment. Its classification must consider its biomechanics, whether it is due to functional muscular imbalance or to structural changes, volition, and intentionality. Due to its varied clinical presentations and different structural alterations, ranging from capsule-labral lesions and bone defects to glenoid dysplasia and retroversion, the different treatment alternatives available have historically had a high incidence of failure. A detailed radiographic assessment, with both CT and MRI, with a precise assessment of glenoid and humeral bone defects and of glenoid morphology, is mandatory. Physiotherapy focused on periscapular muscle reeducation and external rotator strengthening is always the first line of treatment. When conservative treatment fails, surgical treatment must be guided by the structural lesions present, ranging from soft tissue repair to posterior bone block techniques to restore or increase the articular surface. Bone block procedures are indicated in cases of recurrent posterior instability after the failure of conservative treatment or soft tissue techniques, as well as symptomatic demonstrable nonintentional instability, presence of a posterior glenoid defect >10%, increased glenoid retroversion between 10 and 25 degrees, and posterior rim dysplasia. Bone block fixation techniques that avoid screws and metal allow for satisfactory initial clinical results in a safe and reproducible way. An algorithm for the approach and treatment of recurrent posterior glenohumeral instability is presented, as well as the author's preferred surgical technique for arthroscopic posterior bone block

Chlorophyll a fluorescence analysis reveals divergent photosystem II responses to saline, alkaline and saline–alkaline stresses in the two Lotus japonicus model ecotypes MG20 and Gifu-129

Saline and alkaline stresses affect more than 10% of the World’s arable land, limiting agricultural production. Salt-induced stress may affect the photosystem II (PSII) function, altering fluorescence emission. Therefore, changes in fluorescence are used to quantify and analyze abiotic stress responses in plants. So far, no study has focused on the response of PSII to saline, alkaline and saline–alkaline stresses in the model legume Lotus japonicus. For the saline, alkaline and saline–alkaline treatments, plants of the L. japonicus ecotypes MG20 and Gifu-129 were cultivated in sand with nutrient solution, added with NaCl and NaHCO3 in different proportions. Growth, gas exchange, and chlorophyll a fluorescence transient kinetic and OJIP parameters were measured, and chlorophyll a and b were determined. The analysis of the kinetic of chlorophyll a fluorescence showed that NaCl-derived stress sources affect the photochemical events in PSII in both ecotypes, being this effect more evident under higher pH condition, whereas alkalinity per se has a mild or no effect on these events. The saline–alkaline stress induced a more severe effect on Gifu B-129, compared with Miyakojima MG20, whereas NaCl improved primary photochemistry in MG20. Our results allow us to accept the hypothesis that both ecotypes deploy differential responses under the three stressful treatments and that the saline–alkaline stress causes higher damage levels than saline and alkaline stresses alone in relation with structures and sub-processes of the PSII.Fil: Bordenave, César Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Rocco, Ruben Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Maiale, Santiago Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Campestre, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Rodriguez, Andres Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Menendez, Ana Bernardina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentin

CD45 expression discriminates waves of embryonic megakaryocytes in the mouse

Embryonic megakaryopoiesis starts in the yolk sac on gestational day 7.5 as part of the primitive wave of hematopoiesis, and it continues in the fetal liver when this organ is colonized by hematopoietic progenitors between day 9.5 and 10.5, as the definitive hematopoiesis wave. We characterized the precise phenotype of embryo megakaryocytes in the liver at gestational day 11.5, identifying them as CD41++CD45-CD9++CD61+MPL+CD42c+ tetraploid cells that express megakaryocyte-specific transcripts and display differential traits when compared to those present in the yolk sac at the same age. In contrast to megakaryocytes from adult bone marrow, embryo megakaryocytes are CD45- until day 13.5 of gestation, as are both the megakaryocyte progenitors and megakaryocyte/erythroid-committed progenitors. At gestational day 11.5, liver and yolk sac also contain CD41+CD45+ and CD41+CD45- cells. These populations, and that of CD41++CD45-CD42c+ cells, isolated from liver, differentiate in culture into CD41++CD45-CD42c+ proplatelet-bearing megakaryocytes. Also present at this time are CD41-CD45++CD11b+ cells, which produce low numbers of CD41++CD45-CD42c+ megakaryocytes in vitro, as do fetal liver cells expressing the macrophage-specific Csf receptor-1 (Csf1r/CD115) from MaFIA transgenic mice, which give rise poorly to CD41++CD45-CD42c+ embryo megakaryocytes both in vivo and in vitro In contrast, around 30% of adult megakaryocytes (CD41++CD45++CD9++CD42c+) from C57BL/6 and MaFIA mice express CD115. We propose that differential pathways operating in the mouse embryo liver at gestational day 11.5 beget CD41++CD45-CD42c+ embryo megakaryocytes that can be produced from CD41+CD45- or from CD41+CD45+ cells, at difference from those from bone marrow.This work was supported by grants from the Ministerio de Ciencia e Innovacion (MICINN SAF2009-12596) and from the Ministerio de Economia y Competitividad (MINECO SAF2012-33916 and SAF2015-70880-R MINECO/FEDER). NS was the recipient of a fellowship from the Centro de Biologia Molecular Severo Ochoa (CBMSO) and IC received a fellowship from the MICINN. The CBMSO receives institutional funding from Fundacion Ramon Areces. The CNIC is supported by the MEIC and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (MEIC award SEV-2015-0505).S

Bioreactor Platform for Biomimetic Culture and in situ Monitoring of the Mechanical Response of in vitro Engineered Models of Cardiac Tissue

In the past two decades, relevant advances have been made in the generation of engineered cardiac constructs to be used as functional in vitro models for cardiac research or drug testing, and with the ultimate but still challenging goal of repairing the damaged myocardium. To support cardiac tissue generation and maturation in vitro, the application of biomimetic physical stimuli within dedicated bioreactors is crucial. In particular, cardiac-like mechanical stimulation has been demonstrated to promote development and maturation of cardiac tissue models. Here, we developed an automated bioreactor platform for tunable cyclic stretch and in situ monitoring of the mechanical response of in vitro engineered cardiac tissues. To demonstrate the bioreactor platform performance and to investigate the effects of cyclic stretch on construct maturation and contractility, we developed 3D annular cardiac tissue models based on neonatal rat cardiac cells embedded in fibrin hydrogel. The constructs were statically pre-cultured for 5 days and then exposed to 4 days of uniaxial cyclic stretch (sinusoidal waveform, 10% strain, 1 Hz) within the bioreactor. Explanatory biological tests showed that cyclic stretch promoted cardiomyocyte alignment, maintenance, and maturation, with enhanced expression of typical mature cardiac markers compared to static controls. Moreover, in situ monitoring showed increasing passive force of the constructs along the dynamic culture. Finally, only the stretched constructs were responsive to external electrical pacing with synchronous and regular contractile activity, further confirming that cyclic stretching was instrumental for their functional maturation. This study shows that the proposed bioreactor platform is a reliable device for cyclic stretch culture and in situ monitoring of the passive mechanical response of the cultured constructs. The innovative feature of acquiring passive force measurements in situ and along the culture allows monitoring the construct maturation trend without interrupting the culture, making the proposed device a powerful tool for in vitro investigation and ultimately production of functional engineered cardiac constructs

Importancia de la Responsabilidad Social y Empresarial para el Aprovechamiento de los Residuos en la Elaboración de Estructuras Metálicas

En esta actividad final se consolida los procesos de aprendizajes que han generado en cada una de las guías de trabajo establecidas de esta forma se implementaran las estrategias dadas a conocer por los punto expuestos por la escuela de ciencias económicas y contables, en las guías y sus actividades, el grupo de trabajo 23 ha seleccionado una micro empresa, para la construcción que tiene como nombre soluciones mecánicas técnicas y diseño sas, la cual busca una mayor penetración en el mercado aprovechando la aceptación que se tiene en el medio de la construcción generando métodos innovadores en el área de estructuras metálicas con el fin de generar cumplimiento en los procesos y normas que hacen referencia a la responsabilidad social y empresarial. Ahora bien, esto permite tener en conocimiento exacto que se requiere para la elaboración de un proyecto que cumpla con las características idóneas requeridas para presentar este trabajo con proyecto final de graduación, es importante destacar que cada una de las actividades desarrolladas permitió el consolidado éxito de la actividad unidad 9 y 10 fase 5. Desde el principio del diplomado se buscó en consolidado de los ítems que hacen referente a la responsabilidad social y empresarial.In this final activity, the learning processes that have been generated in each of the established work guides are consolidated. In this way, the strategies made known by the points presented by the national open and distance university in the activity guides will be implemented, the working group 23 has selected a micro company, for construction whose name is technical mechanical solutions and design sas, which seeks greater penetration in the market taking advantage of the acceptance that exists in the construction environment, generating innovative methods in the area of metal structures in order to generate compliance in the processes and standards that refer to social and business responsibility. Now, this allows us to have exact knowledge of what is required for the development of a project that meets the ideal characteristics required to present this work with a final graduation project. It is important to highlight that each of the activities developed allowed the consolidated success of the activity unit 9 and 10 phase 5. From the beginning of the diploma, a consolidated search was made for the items that refer to social and business responsibility

Diagrama de redes y ruta crítica aplicado al casco estructural de una edificación escolar

El diagrama de redes y ruta crítica es un método de planificación utilizado desde 1956 en diversos sectores, no solo en el de construcción. Su eficiencia y simplicidad ha sido tal que hasta la fecha actual se mantiene el uso de este método. Mediante la elaboración del presente documento, se quiere comprobar la capacidad de sintetización y orden que esta ofrece; así mismo, evaluar la eficiencia de aplicación en proyectos mayores a un nivel y constatar que es una excelente herramienta de control y seguimiento en proyectos de construcción. Así mismo, el desarrollo de la investigación contempla la elaboración del diagrama de redes y ruta crítica para la etapa del casco estructural del proyecto de un pabellón escolar. Se plantean tres escenarios: bajo condiciones normales, con un retraso en partidas que afectan la duración total del proyecto y por último asumiendo de igual forma el retraso del segundo modelo, pero, reasignando recursos, modo tal que los rendimientos sean mayores y concluya el proyecto en el tiempo planificado (duración del primer modelo). Como resultados se obtuvieron los siguientes tiempos de ejecución: para el primer caso 146 días, para el segundo caso 150 días y para el tercer caso, de igual modo que el primero, 146 días. Cabe destacar que el estudio se apoya en el uso de programas informáticos de planificación como S10 y Project; los cuales permiten que la elaboración de este método sea sencilla y rápida, volviéndolo aún más eficaz y adaptable en el tiempo