Connecting Middle Palaeolithic datasets: The interplay of zooarchaeological and lithic data for unravelling Neanderthal behaviour

The ongoing refinement of archaeological excavation and recording methods over the last decades has led to a significant increase in quantitative Middle Palaeolithic datasets that provide a record of past Neanderthal behaviour. Stone tools and butchered animal remains are the two main categories of Middle Palaeolithic archaeological remains and both provide distinctive insights into site formation and Neanderthal behaviour. However, the integration of these quantitative lithic and zooarchaeological datasets is key for achieving a full understanding of both site-specific and broader-scale patterns of Middle Palaeolithic subsistence. To explore novel ways to enhance the incorporation of these datasets, we organised a session at the 82nd annual meeting of the Society for American Archaeology in Vancouver. An underlying theme was the problem of linking lithic and faunal variability. Are variations in subsistence strategies reflected by changes in toolmaking decisions? This paper will briefly introduce the possible ways these Middle Palaeolithic datasets can be integrated, illustrated with the papers included in this special volume, and discuss its potential for understanding the variability and interconnectedness of Neanderthal technologies and subsistence strategies

When lithics hit bones: Evaluating the potential of a multifaceted experimental protocol to illuminate Middle Palaeolithic weapon technology

Recent zooarchaeological and isotope analyses have largely settled the debate surrounding Neanderthal hunting capacities, repeatedly demonstrating their successful acquisition of large ungulates. Nevertheless, the functional identification of individual tools as hunting weapons remains a methodological challenge. In-depth studies have focussed mainly on small subsets of lithic artefacts from selected assemblages assessing features of breakage patterns, retouch, shape and use wear. Studies focussing on associated hunting lesions are rarer and often focus on reconstructing very specific bone surface marks encountered in the archaeological record. This study aims to add to our understanding of the formation and characteristics of projectile impact marks (PIMs) on bone through a series of highly monitored, replicative experiments, using thrusting and throwing spears with replica Levallois points into two wild pig carcasses. In total, 152 shots were made, and for each a series of attributes was recorded, including velocity and location of impact. Subsequent quantitative analyses focussed on understanding the various factors underlying the formation of different types of projectile impact marks. These experiments demonstrate that PIM formation results from the properties of both the impacting projectile and bone element. PIMs can signal impacts caused by different delivery methods but only on some parts of the skeleton. These results are contextualised in relation to the occurrence and recognition of Palaeolithic PIMs and patterns of Neanderthal behaviour. These experiments are only a first step in improving the recognition of these signatures in the archaeological record and providing better insights into understanding of the mechanisms of Neanderthal hunting

The Late Middle Palaeolithic Occupation of Abri du Maras (Layer 1, Neronian, Southeast France): Integrating Lithic Analyses, ZooMS and Radiocarbon Dating to Reconstruct Neanderthal Hunting Behaviour

The exact strategies and technologies underlying Neanderthal hunting events remain open for debate with lithic points being sparse across the European Middle Palaeolithic. An exception is the Neronian entity in southeast France, defined by ventrally retouched Soyons points. This study contextualises one of the largest Neronian assemblages, layer 1 at Abri du Maras. Our lithic analyses focussed on attributes described as indicative of projectile use or hafting to contextualise the morphometric and technological characteristics of the pointed implements at an assemblage level. We found that retouched points were made on a variety of blank types (including Levallois, laminar and discoidal flaking techniques) and ventral retouch is present across different artefact types (including points, scrapers and denticulates). Next, these lithic data were compared to similar typo-technological data recorded on a sample from the recently excavated and well-contextualised point-rich layer 4.1 of Abri du Maras (MIS-3). Zooarchaeology by Mass Spectrometry (ZooMS) was applied to 280 faunal remains from layer 1 and indicated significant proportions of equids, bovids, cervids and reindeer. Carnivore remains and carnivore modifications are absent, while human bone surface modifications are present across a variety of species. Five bones had sufficient collagen for radiocarbon dating but returned dates younger than expected (ca. 41–31 ka cal BP). Finally, we place Abri du Maras layer 1 in its broader regional context and discuss its relation to other Neronian assemblages and more general problematics inherent to studying material from old excavations

Increasing sustainability in palaeoproteomics by optimizing digestion times for large-scale archaeological bone analyses

Palaeoproteomic analysis of skeletal proteomes is used to provide taxonomic identifications for an increasing number of archaeological specimens. The success rate depends on a range of taphonomic factors and differences in the extraction protocols employed. By analyzing 12 archaeological bone specimens from two archaeological sites, we demonstrate that reducing digestion duration from 18 to 3 hours has no measurable impact on the obtained taxonomic identifications. Peptide marker recovery, COL1 sequence coverage, or proteome complexity are also not significantly impacted. Although we observe minor differences in sequence coverage and glutamine deamidation, these are not consistent across our dataset. A 6-fold reduction in digestion time reduces electricity consumption, and therefore CO2 emission intensities. We furthermore demonstrate that working in 96-well plates further reduces electricity consumption by 60%, in comparison to individual microtubes. Reducing digestion time therefore has no impact on the taxonomic identifications, while reducing the environmental impact of palaeoproteomic projects

Ramification of lithic production and the search of small tools in Iberian Peninsula Middle Paleolithic

The notion of recycling and it relationship with ramified productions and small tool production in Late Middle Paleolithic from the Iberian Peninsula are investigated. Results from Amalda, Axlor, Pe~na Miel, and Quebrada show that the production of small tools is one of the principal objectives of lithic provisioning in these sites. Whereas in Axlor and Amalda, this is achieved through the ramification of production, due to the remoteness of flint sources, in Quebrada, where raw material sources are closer, small flakes are obtained at the end of Levallois production. The implications for Neandertal society organization of this small tool production is discussed, and its evolution is observed from a diachronic perspective

The ecology, subsistence and diet of ~45,000-year-old Homo sapiens at Ilsenhöhle in Ranis, Germany

Recent excavations at Ranis (Germany) identified an early dispersal of Homo sapiens into the higher latitudes of Europe by 45,000 years ago. Here we integrate results from zooarchaeology, palaeoproteomics, sediment DNA and stable isotopes to characterize the ecology, subsistence and diet of these early H. sapiens. We assessed all bone remains (n = 1,754) from the 2016-2022 excavations through morphology (n = 1,218) or palaeoproteomics (zooarchaeology by mass spectrometry (n = 536) and species by proteome investigation (n = 212)). Dominant taxa include reindeer, cave bear, woolly rhinoceros and horse, indicating cold climatic conditions. Numerous carnivore modifications, alongside sparse cut-marked and burnt bones, illustrate a predominant use of the site by hibernating cave bears and denning hyaenas, coupled with a fluctuating human presence. Faunal diversity and high carnivore input were further supported by ancient mammalian DNA recovered from 26 sediment samples. Bulk collagen carbon and nitrogen stable isotope data from 52 animal and 10 human remains confirm a cold steppe/tundra setting and indicate a homogenous human diet based on large terrestrial mammals. This lower-density archaeological signature matches other Lincombian-Ranisian-Jerzmanowician sites and is best explained by expedient visits of short duration by small, mobile groups of pioneer H. sapiens. [Abstract copyright: © 2024. The Author(s).

Stable isotopes show Homo sapiens dispersed into cold steppes ~45,000 years ago at Ilsenhöhle in Ranis, Germany

The spread of Homo sapiens into new habitats across Eurasia ~45,000 years ago and the concurrent disappearance of Neanderthals represents a critical evolutionary turnover in our species' history. 'Transitional' technocomplexes, such as the Lincombian-Ranisian-Jerzmanowician (LRJ), characterize the European record during this period but their makers and evolutionary significance have long remained unclear. New evidence from Ilsenhöhle in Ranis, Germany, now provides a secure connection of the LRJ to H. sapiens remains dated to ~45,000 years ago, making it one of the earliest forays of our species to central Europe. Using many stable isotope records of climate produced from 16 serially sampled equid teeth spanning ~12,500 years of LRJ and Upper Palaeolithic human occupation at Ranis, we review the ability of early humans to adapt to different climate and habitat conditions. Results show that cold climates prevailed across LRJ occupations, with a temperature decrease culminating in a pronounced cold excursion at ~45,000-43,000 cal BP. Directly dated H. sapiens remains confirm that humans used the site even during this very cold phase. Together with recent evidence from the Initial Upper Palaeolithic, this demonstrates that humans operated in severe cold conditions during many distinct early dispersals into Europe and suggests pronounced adaptability. [Abstract copyright: © 2024. The Author(s).

Homo sapiens reached the higher latitudes of Europe by 45,000 years ago

The Middle to Upper Palaeolithic transition in Europe is associated with the regional disappearance of Neanderthals and the spread of Homo sapiens. Late Neanderthals persisted in western Europe several millennia after the occurrence of H. sapiens in eastern Europe1. Local hybridization between the two groups occurred2, but not on all occasions3. Archaeological evidence also indicates the presence of several technocomplexes during this transition, complicating our understanding and the association of behavioural adaptations with specific hominin groups4. One such technocomplex for which the makers are unknown is the Lincombian–Ranisian–Jerzmanowician (LRJ), which has been described in northwestern and central Europe5,6,7,8. Here we present the morphological and proteomic taxonomic identification, mitochondrial DNA analysis and direct radiocarbon dating of human remains directly associated with an LRJ assemblage at the site Ilsenhöhle in Ranis (Germany). These human remains are among the earliest directly dated Upper Palaeolithic H. sapiens remains in Eurasia. We show that early H. sapiens associated with the LRJ were present in central and northwestern Europe long before the extinction of late Neanderthals in southwestern Europe. Our results strengthen the notion of a patchwork of distinct human populations and technocomplexes present in Europe during this transitional period