Site of semen deposition and fertility in lactating beef cows synchronized with GnRH and PGF2α

Our objective was to determine the effect of site of semen deposition on pregnancy rate in beef cows inseminated at a fixed time or after observed estrus. Cows were synchronized with a combination of gonadotropin-releasing hormone (GnRH) prostaglandin-F α (PGF). GnRH was injected 7 days before PGF (day 0; first of breeding season). The trial was conducted at two locations, one in Kansas (147 cows) and one in Colorado (313 cows). At each location, cows were assigned to be inseminated after observed estrus (ESTRUS-AI) or at a fixed time (TIMED-AI). Within these two groups, cows either were inseminated in the uterine body (BODY-bred) or in both uterine horns (HORN-bred). Cows in the ESTRUS-AI group were observed for estrus each morning and evening until day 5 afterPGF and then inseminated 12 hr after first detected estrus. Cows in the TIMED-AI group received a second dose of GnRH on day 2 and were inseminated at that time (48 to 56 hr after PGF). Heat response, AI conception rate, and pregnancy rate were analyzed for BODY-bred and HORN-bred cows within each treatment at each location. No differences in these variables occurred between locations, so the results were combined. Within the ESTRUS-AI group, neither conception rate (70% vs. 73%) nor pregnancy rate (39% vs. 40%) was different between BODY-bred and HORN-bred cows respectively. Pregnancy rate within the TIMED-AI group tended (P=.09) to be greater for BODY-bred (53%) compared to HORN-bred (42%) cows. When BODY-bred and HORN-bred treatments were combined, the pregnancy rate of TIMED-AI cows (48%) tended (P=.07) to be greater than that of ESTRUS-AI cows (39%). Timed-insemination resulted in a greater pregnancy rate than inseminating cows according to estrus. No advantage was seen in conception rates when semen was deposited in the uterine horns compared to the uterine body

Sparse Coding Predicts Optic Flow Specificities of Zebrafish Pretectal Neurons

Zebrafish pretectal neurons exhibit specificities for large-field optic flow patterns associated with rotatory or translatory body motion. We investigate the hypothesis that these specificities reflect the input statistics of natural optic flow. Realistic motion sequences were generated using computer graphics simulating self-motion in an underwater scene. Local retinal motion was estimated with a motion detector and encoded in four populations of directionally tuned retinal ganglion cells, represented as two signed input variables. This activity was then used as input into one of two learning networks: a sparse coding network (competitive learning) and backpropagation network (supervised learning). Both simulations develop specificities for optic flow which are comparable to those found in a neurophysiological study (Kubo et al. 2014), and relative frequencies of the various neuronal responses are best modeled by the sparse coding approach. We conclude that the optic flow neurons in the zebrafish pretectum do reflect the optic flow statistics. The predicted vectorial receptive fields show typical optic flow fields but also "Gabor" and dipole-shaped patterns that likely reflect difference fields needed for reconstruction by linear superposition.Comment: Published Conference Paper from ICANN 2018, Rhode

Surgery for Port-Wine Stains: A Systematic Review

Background: Port-wine stains (PWS) are congenital low-flow vascular malformations of the skin. PWS tend to become thicker and darker with time. Laser therapy is the gold standard and the first-line therapy for treating PWS. However, some resistant PWS, or PWS that have tissue hypertrophy, do not respond to this therapy. Our aim is to evaluate the role of surgery in the treatment of PWS birthmarks. Methods: A literature search was performed in PubMed, Scopus, Web of Science (WOS) and Google Scholar for all papers dealing with surgery for port-wine stains, from January 2010 to December 2020 using the search strings: (capillary vascular malformation OR port-wine stains OR Sturge Weber Syndrome OR sws OR pws) AND (surgical OR surgery). Results: Ten articles were identified and used for analysis. They were almost all case series with a short follow up period and lacked an objective–systematic score of evaluation. Conclusions: Delay in treatment of port wine stains may result in soft tissue and bone hypertrophy or nodules with disfiguring or destructive characteristics. The correction of PWS-related facial asymmetry often requires bone surgery followed by soft tissue corrections to achieve a more harmonious, predictable result

Speakers and the Spotlight: Explaining Media Coverage of Leadership in the House of Representatives

Previous scholarship has described how members of Congress struggle to attract media attention. The Speaker of the House, however, is uniquely positioned to drive media agendas. That position may be conditioned though by the framework in which the Speaker operates, shocks to the system of leadership in the House, and the process of legislating. We inquire into the conditions under which the Speaker of the House receives increased or decreased attention from newspapers, network newscasts, and cable newscasts. We also examine the contraction and expansion of the gap between levels of presidential and Speaker coverage. We find that Speaker coverage is often shaped by increases in House polarization, changes in which party holds the position, Speaker scandal, and turning bills into laws.Yeshttps://us.sagepub.com/en-us/nam/manuscript-submission-guideline

Multiple General Anesthesia in Children: A Systematic Review of Its Effect on Neurodevelopment

The effect of multiple general anesthesia (mGA) procedures administered in early life is a critical theme and has led the Food and Drug Administration (FDA) to issue an alert. This systematic review seeks to explore the potential effects on neurodevelopment of mGA on patients under 4 years. The Medline, Embase and Web of Science databases were searched for publications up to 31 March 2021. The databases were searched for publications regarding “children multiple general anesthesia OR pediatric multiple general anesthesia”. Case reports, animal studies and expert opinions were excluded. Systematic reviews were not included, but they were screened to identify any possible additional information. A total of 3156 studies were identified. After removing the duplicates, screening the remaining records and analyzing the systematic reviews’ bibliography, 10 studies were considered suitable for inclusion. Comprehensively, a total cohort of 264.759 unexposed children and 11.027 exposed children were assessed for neurodevelopmental outcomes. Only one paper did not find any statistically significant difference between exposed and unexposed children in terms of neurodevelopmental alterations. Controlled studies on mGA administered before 4 years of age support that there might be a greater risk of neurodevelopmental delay in children receiving mGA, warranting the need for careful risk/benefit considerations

An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells

Both human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) bear a great potential in regenerative medicine. In addition to optimized clinical grade culture conditions, efficient clinical grade cryopreservation methods for these cells are needed. Obtaining good survival after thawing has been problematic

The cellular and synaptic architecture of the mechanosensory dorsal horn

The deep dorsal horn is a poorly characterized spinal cord region implicated in processing low-threshold mechanoreceptor (LTMR) information. We report an array of mouse genetic tools for defining neuronal components and functions of the dorsal horn LTMR-recipient zone (LTMR-RZ), a role for LTMR-RZ processing in tactile perception, and the basic logic of LTMR-RZ organization. We found an unexpectedly high degree of neuronal diversity in the LTMR-RZ: seven excitatory and four inhibitory subtypes of interneurons exhibiting unique morphological, physiological, and synaptic properties. Remarkably, LTMRs form synapses on between four and 11 LTMR-RZ interneuron subtypes, while each LTMR-RZ interneuron subtype samples inputs from at least one to three LTMR classes, as well as spinal cord interneurons and corticospinal neurons. Thus, the LTMR-RZ is a somatosensory processing region endowed with a neuronal complexity that rivals the retina and functions to pattern the activity of ascending touch pathways that underlie tactile perception

Competing for the Platform: How Organized Interests affect Party Positioning in the United States

What explains which groups are included in a party coalition in any given election cycle? Recent advances in political party theory suggest that policy demanders comprise parties, and that the composition of a party coalition varies from election to election. We theorize three conditions under which parties articulate an interest group?s preferred positions in its quadrennial platform: when groups are ideologically proximate to the party median, when groups display party loyalty, and when groups are flush with resources. Using computer-assisted content analysis on a unique and rich data source, we examine three cycles of testimony that 80 organized groups provided to the Democratic Party. The analysis compares group requests with the content of Democratic and Republican National Committee platforms in 1996, 2000, and 2004. Results show that parties reward loyal groups and those that are ideologically proximate to the party, but offer no confirmation of a resource effect

Tight p-fusion frames

Fusion frames enable signal decompositions into weighted linear subspace components. For positive integers p, we introduce p-fusion frames, a sharpening of the notion of fusion frames. Tight p-fusion frames are closely related to the classical notions of designs and cubature formulas in Grassmann spaces and are analyzed with methods from harmonic analysis in the Grassmannians. We define the p-fusion frame potential, derive bounds for its value, and discuss the connections to tight p-fusion frames