All for Him: Articles About Sex in American Lad Magazines

Research on sources of information about sex indicates that independent reading is a primary source of information for many young people. For young men, such reading is likely to involve lad magazines, a relatively new but very popular genre of lifestyle magazines targeted at young men. In the present study, a systematic representative sample of articles about sex from American lad magazines was subjected to content analysis. Results suggest that, although many articles focus on what women want sexually, the overall message of these articles privileges a fairly narrow male sexuality oriented toward sexual variety. At the same time, the normative relational context of sex is depicted as serious dating relationships. These results are consistent with predominant gender stereotypes about sex.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/45640/1/11199_2005_Article_1291.pd

Does Television Exposure Affect Emerging Adults' Attitudes and Assumptions About Sexual Relationships? Correlational and Experimental Confirmation

Because concern is frequently raised that TV's abundant yet stereotypical portrayals of sexual relationships may mislead young viewers, this study examined the effects of both regular viewing habits and experimental exposure on students' conceptions about sexual relationships. A multiethnic sample of 259 undergraduates aged 18–22 was assigned to view a set of clips depicting either 1 of 3 sexual stereotypes or neutral, nonsexual content. Participants then completed measures assessing their attitudes about sexual roles and relationships, their assumptions about the sexual experiences of their peers, and their regular viewing habits. Both correlational and experimental connections emerged between TV viewing and students' sexual attitudes and assumptions. More frequent and more involved viewing were repeatedly associated with students' support of the sexual stereotypes surveyed. Similarly, women exposed to clips representing a particular sexual stereotype were more likely to endorse that notion than were women exposed to nonsexual content. Finally, both experimental exposure and aspects of regular viewing significantly predicted students' sexual attitudes and assumptions, even with demographics and previous sexual experiences controlled.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/45292/1/10964_2004_Article_364602.pd

Molecular and functional interactions between AKT and SOX2 in breast carcinoma

The transcription factor SOX2 is a key regulator of pluripotency in embryonic stem cells and plays important roles in early organogenesis. Recently, SOX2 expression was documented in various cancers and suggested as a cancer stem cell (CSC) marker. Here we identify the Ser/Thr-kinase AKT as an upstream regulator of SOX2 protein turnover in breast carcinoma (BC). SOX2 and pAKT are co-expressed and co-regulated in breast CSCs and depletion of either reduces clonogenicity. Ectopic SOX2 expression restores clonogenicity and in vivo tumorigenicity of AKT-inhibited cells, suggesting that SOX2 acts as a functional downstream AKT target. Mechanistically, we show that AKT physically interacts with the SOX2 protein to modulate its subcellular distribution. AKT kinase inhibition results in enhanced cytoplasmic retention of SOX2, presumably via impaired nuclear import, and in successive cytoplasmic proteasomal degradation of the protein. In line, blockade of either nuclear transport or proteasomal degradation rescues SOX2 expression in AKT-inhibited BC cells. Finally, AKT inhibitors efficiently suppress the growth of SOX2-expressing putative cancer stem cells, whereas conventional chemotherapeutics select for this population. Together, our results suggest the AKT/SOX2 molecular axis as a regulator of BC clonogenicity and AKT inhibitors as promising drugs for the treatment of SOX2-positive BC

Common variants of the <i>BRCA1</i> wild-type allele modify the risk of breast cancer in <i>BRCA1</i> mutation carriers

Mutations in the &lt;i&gt;BRCA1&lt;/i&gt; gene substantially increase a woman's lifetime risk of breast cancer. However, there is great variation in this increase in risk with several genetic and non-genetic modifiers identified. The &lt;i&gt;BRCA1&lt;/i&gt; protein plays a central role in DNA repair, a mechanism that is particularly instrumental in safeguarding cells against tumorigenesis. We hypothesized that polymorphisms that alter the expression and/or function of &lt;i&gt;BRCA1&lt;/i&gt; carried on the wild-type (non-mutated) copy of the &lt;i&gt;BRCA1&lt;/i&gt; gene would modify the risk of breast cancer in carriers of &lt;i&gt;BRCA1&lt;/i&gt; mutations. A total of 9874 &lt;i&gt;BRCA1&lt;/i&gt; mutation carriers were available in the Consortium of Investigators of Modifiers of &lt;i&gt;BRCA1/2&lt;/i&gt; (CIMBA) for haplotype analyses of &lt;i&gt;BRCA1&lt;/i&gt;. Women carrying the rare allele of single nucleotide polymorphism rs16942 on the wild-type copy of &lt;i&gt;BRCA1&lt;/i&gt; were at decreased risk of breast cancer (hazard ratio 0.86, 95% confidence interval 0.77–0.95, &lt;i&gt;P&lt;/i&gt; = 0.003). Promoter &lt;i&gt;in vitro&lt;/i&gt; assays of the major &lt;i&gt;BRCA1&lt;/i&gt; haplotypes showed that common polymorphisms in the regulatory region alter its activity and that this effect may be attributed to the differential binding affinity of nuclear proteins. In conclusion, variants on the wild-type copy of &lt;i&gt;BRCA1&lt;/i&gt; modify risk of breast cancer among carriers of &lt;i&gt;BRCA1&lt;/i&gt; mutations, possibly by altering the efficiency of &lt;i&gt;BRCA1&lt;/i&gt; transcription