Improving Students\u27 Reading Comprehension on Narrative Text by Using Story Map Technique

: This thesis is a study on improving students\u27 reading comprehension in narrative text by using Story Map Technique to the tenth grade students of SMA Negeri 10 Pontianak in academic year 2014/2015. The writer took a classroom action research and the teaching learning process took place in two cycles. The subjects of this research were the students of grade X IPA 3 which consist of 39 students. From the observation checklist and field notes, it was found that all the students participated to the activity and did their task well. The students gave positive response to all the explanation and could use the story map technique effectively. For these results, it was concluded that story map technique improved students\u27 reading comprehension skill in narrative text in their Story Map Technique enabled the students to store information in their personal schema more efficiently and facilitated to recall the story elements more completely and accurately

Parenting Patterns Of Parents And Mental-emotional Development In Taman Kanak-kanak Dharma Wanita Godean, Nganjuk District

Background: Growth and development on preschooler is the basic step that influenced the next development on their life thus it was called golden period. Preschooler's psychosocial development related on their mental process. One of the factor that influenced mental-emotional development is the lacked of parenatal affection or warmth and care to the children.Objectives: This study aimed to describe and examine the correlation between parenting patterns and mental-emotional development at Taman Kanak-kanak Dharma Wanita Godean, Nganjuk District.Method: Correlation method with case control approched was utilized on this research and implemented on March 27th of 2018 throughout March 29th of 2018 at Taman Kanak-kanak Dharma Wanita Godean Kabupaten Nganjuk. All preschoolers parents as many as 47 couple (94 people) at this Taman Kanak-kanak Dharma Wanita Godean Kabupaten Nganjuk was defined as the population sampling. This research utilized purposive sampling thus as many as 40 parents matched the inclusion and exclusion criteria. The parenting was independent variable and the mental-emotional development was the dependent variable. Statistical test used Spearmen Rho test.Results: The outcome of the study was found that most parenting parents of 23 parents (57,5 %) had an authoritative parenting type, most of which were 23 elderly (57,5 %) was uneble to development mental-emotional approach and nearly half of the 18 parents (45 %) have authoritative parenting style and unable to develop mental-emotional approach, alternate hypothesis (Ha) is accepted with rho value of 0,011 less than alpha value of 0,05 (rho= 0,001 ≤ α= 0,05).Conclusion: Appropriate parenting corresponds child's growth and influenced mental-emotional child's development, parenting efficacy can be utilized for preventive measure on preschooler mental-emotional disorder

Розробка та валідація методики визначення супровідних домішок у таблетках рибоксин

Riboxin (inosine) is used in medicine for treating cardiovascular diseases, so the number of dosage forms containing this substance as an active ingredient is constantly increasing at the Ukrainian pharmaceutical market. When developing quality control methods for a dosage form containing one active substance it is advisable to carry out tests on the presence of related impurities besides identification and assay tests. The article presents the data concerning development of the method for determination of related impurities (hypoxanthine, guanosine) in riboxine tablets using HPLC. The analysis was carried out using a HPLC column (125×4 mm i.d., 5 μm particles) filled with “Lichrospher 60 RP select B”sorbent. The mobile phase adjusted with the phosphate buffer to pH 3.5 was used. The UV detection was performed at 250 nm. The validation characteristics have been studied using the acceptance criteria for the tolerances of the content not more than 0.5% for each impurity, and they confirm specificity (the absence of the effect of excipients), linearity, precision (convergence), accuracy (Δz = 0.79≤max Δz = 5.0, δ = 0.21≤max δ = 0.26, a = 0.48, r = 0.99997>0.9976 for hypoxanthine, and Δz = 0.83≤max Δz = 5.0, δ = 0.17≤max δ = 0.28, a = 0.21, r = 0.99997>0.9976 for guanosine), and the application range for the method proposed.Рибоксин (инозин) применяют в медицинской практике для лечения сердечно-сосудистых заболеваний, поэтому количество готовых лекарственных форм, содержащих в качестве активного фармацевтического ингредиента именно это вещество, на фармацевтическом рынке Украины постоянно растет. При разработке методик контроля качества на готовую лекарственную форму, содержащую одно действующее вещество, целесообразно кроме испытаний на идентификацию и количественное определение также проводить испытания на содержание сопутствующих примесей. В предлагаемой работе приводятся данные по разработке методики определения сопутствующих примесей (гипоксантин, гуанозин) рибоксина в таблетках с использованием метода высокоэффективной жидкостной хроматографии. Определение проводили на колонке размером 125×4 мм, заполненной сорбентом «Lichrospher 60 RP select B» с размером частиц 5 мкм, использовали подвижную фазу на основе фосфатного буферного раствора с рН 3,5 и УФ-детектирование при длине волны 250 нм. Изученные валидационные характеристики с использованием критериев приемлемости для допусков содержания не более 0,5% для каждой из примесей подтверждают специфичность (отсутствие влияния вспомогательных веществ), линейность, прецизионность (сходимость), правильность (Δz = 0.79≤max Δz = 5.0, δ = 0.21≤max δ = 0.26, a = 0.48, r = 0.99997 гипоксантина и Δz = 0.83≤max Δz = 5.0, δ = 0.17≤max δ = 0.28, a = 0.21, r = 0.99997 гуанозина) и диапазон применения предлагаемой методики.Рибоксин (інозин) застосовують у медичній практиці для лікування серцево-судинних захворювань, тому кількість готових лікарських форм, що містять як активний фармацевтичний інгредієнт саме цю речовину, на фармацевтичному ринку України постійно зростає. При розробці методик контролю якості на готову лікарську форму, що містить одну діючу речовину, доцільно окрім випробувань на ідентифікацію та кількісне визначення також проводити випробування на вміст супутніх домішок. У запропонованій роботі наводяться дані по розробці методики визначення супровідних домішок (гіпоксантин, гуанозин) рибоксину в таблетках з використанням методу високоефективної рідинної хроматографії. Визначення проводили на колонці розміром 125×4 мм, заповненій сорбентом «Lichrospher 60 RP select B» з розміром часток 5 мкм, використовували рухому фазу на основі фосфатного буферного розчину з рН 3,5 та УФ-детектування за довжини хвилі 250 нм. Вивчені валідаційні характеристики з використанням критеріїв прийнятності для допусків вмісту не більше 0,5% для кожної з домішок підтверджують специфічність (відсутність впливу допоміжних речовин), лінійність, прецизійність (збіжність), правильність (Δz = 0.79≤max Δz = 5.0, δ = 0.21≤max δ = 0.26, a = 0.48, r = 0.99997>0.9976 гіпоксантину та Δz = 0.83≤max Δz = 5.0, δ = 0.17≤max δ = 0.28, a = 0.21, r = 0.99997>0.9976 гуанозину) та діапазон застосування запропонованої методики

Deep inorganic fraction characterization of PM10, PM2.5, AND PM1 in an industrial area located in central italy by means of instrumental neutron activation analysis

Atmospheric pollution is an important task in life sciences and, in particular, inorganic fraction characterization is considered as an important issue in this field. For many years, researchers have focused their attention on the particulate matter fraction below 10 μm: in this case, our attention was also focused on PM2.5 (i.e., particles with a size fraction smaller than 2.5 μm) and PM1 (below 1 μm). This paper would like to investigate whether the element accumulation in different granulometric fractions is similar, or whether there are behavior dissimilarities. Among the different analytical techniques, the instrumental neutron activation analysis, an instrumental nuclear method, was used for its peculiarity of investigating the sample without performing any chemical-physical treatment. Forty-two daily samples using the reference method were collected, 15 filters for PM10, 18 for PM2.5, and 12 for PM1; the filters, along with primary standards and appropriate standard reference materials, were irradiated at the National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA) R.C.-Casaccia's Triga MARK II reactor. The irradiations carried out in the Rabbit and Lazy Susan channels allowed for the investigation of 36 elements and the relative Pearson's correlations between elements and PM-fractions (PM10 vs. PM2.5 was good, whereas PM10 vs. PM1 was the worst). The Enrichment Factors were studied for the three fractions to show how anthropogenic sources have affected the element content. A comparison between these data and element levels determined worldwide showed that our concentrations were lower than those determined in similar scenarios. Furthermore, a statistical approach (source discrimination, hierarchical cluster analysis, principal component analysis) has allowed us to identify similarities between the samples: the airborne filters can be divided in two main groups (i.e., one made of PM10 and PM2.5 filters and one only of PM1 filters), meaning a different element contribution to this fraction coming from other sources present at the site

Tissue distribution of a plasmid DNA encoding Hsp65 gene is dependent on the dose administered through intramuscular delivery

In order to assess a new strategy of DNA vaccine for a more complete understanding of its action in immune response, it is important to determine the in vivo biodistribution fate and antigen expression. In previous studies, our group focused on the prophylactic and therapeutic use of a plasmid DNA encoding the Mycobacterium leprae 65-kDa heat shock protein (Hsp65) and achieved an efficient immune response induction as well as protection against virulent M. tuberculosis challenge. In the present study, we examined in vivo tissue distribution of naked DNA-Hsp65 vaccine, the Hsp65 message, genome integration and methylation status of plasmid DNA. The DNA-Hsp65 was detectable in several tissue types, indicating that DNA-Hsp65 disseminates widely throughout the body. The biodistribution was dose-dependent. In contrast, RT-PCR detected the Hsp65 message for at least 15 days in muscle or liver tissue from immunized mice. We also analyzed the methylation status and integration of the injected plasmid DNA into the host cellular genome. The bacterial methylation pattern persisted for at least 6 months, indicating that the plasmid DNA-Hsp65 does not replicate in mammalian tissue, and Southern blot analysis showed that plasmid DNA was not integrated. These results have important implications for the use of DNA-Hsp65 vaccine in a clinical setting and open new perspectives for DNA vaccines and new considerations about the inoculation site and delivery system

Validity of the Empatica E4 wristband to measure heart rate variability (HRV) parameters:A comparison to electrocardiography (ECG)

Wearable monitoring devices are an innovative way to measure heart rate (HR) and heart rate variability (HRV), however, there is still debate about the validity of these wearables. This study aimed to validate the accuracy and predictive value of the Empatica E4 wristband against the VU University Ambulatory Monitoring System (VU-AMS) in a clinical population of traumatized adolescents in residential care. A sample of 345 recordings of both the Empatica E4 wristband and the VU-AMS was derived from a feasibility study that included fifteen participants. They wore both devices during two experimental testing and twelve intervention sessions. We used correlations, cross-correlations, Mann-Whitney tests, difference factors, Bland-Altman plots, and Limits of Agreement to evaluate differences in outcomes between devices. Significant correlations were found between Empatica E4 and VU-AMS recordings for HR, SDNN, RMSSD, and HF recordings. There was a significant difference between the devices for all parameters but HR, although effect sizes were small for SDNN, LF, and HF. For all parameters but RMSSD, testing outcomes of the two devices led to the same conclusions regarding significance. The Empatica E4 wristband provides a new opportunity to measure HRV in an unobtrusive way. Results of this study indicate the potential of the Empatica E4 as a practical and valid tool for research on HR and HRV under non-movement conditions. While more research needs to be conducted, this study could be considered as a first step to support the use of HRV recordings provided by wearables

“Mura della Bastia” – Onigo di Pederobba (Treviso). Ricerche archeologiche, rilievo 3D laser scanning e anastilosi virtuale in un castello medievale del pedemonte trevigiano

The paper presents the results of the ongoing research coordinated by the Chair of Ancient Topography at the University of Padova. The work concerns the medieval site of “Mura della Bastia” in Onigo di Pederobba (Treviso), in the North-Eastern part of Italy. Historical information from written documents and results obtained from the archaeological investigation at the Onigo castle (1994-2007) are discussed. The study provided the historical reconstruction of recent collapses of the Onigo tower and allowed the virtual reconstruction of still-cohesive blocks, which had fallen down in 1989, and which were recovered during the summer of 2007. At this time, five of the ten building blocks have been positioned; the procedure was carried out by comparing the panoply of each agglomerate with the prospect of the tower, which was obtained from the photogrammetric rectification of the historical images (2D). The 3D survey of the tower and of nine blocks was performed with a Leica HDS 2500 laser scanner. The resulting point clouds were aligned with Cyclone software, also using the coordinates of some laser scanning targets, which were measured with the Leica TC 2003 total station in a local reference system. The final 3D model obtained by the co-registration of the scans was textured with the digital images acquired with a Canon EOS 1 ds Mark II camera. Subsequently, three of the five recognized blocks were positioned on the 3D model using Reconstructor software