6 research outputs found
Real-Time Polymerase Chain Reaction in Stool Detects Transmission of Strongyloides stercoralis from an Infected Donor to Solid Organ Transplant Recipients
Solid organ transplant recipients can acquire Strongyloides
stercoralis from an infected donor. The diagnosis of S.
stercoralis in immunocompromised individuals may be challenging
due to a lower sensitivity of available parasitological and
serological methods, compared with immunocompetent individuals.
Recently, a real-time polymerase chain reaction (RT-PCR) in
stool has been developed for S. stercoralis diagnosis. We report
two cases of S. stercoralis infection transmitted by a donor to
two solid organ transplant recipients, whose stool samples were
diagnosed using RT-PCR. This test could play an important role
in S. stercoralis diagnosis in immunosuppressed patients,
facilitating rapid treatment initiation and reducing the risk of
severe strongyloidiasis. Adherence to current recommendations of
screening among donors and recipients from endemic areas is also
urgently needed
Real-Time Polymerase Chain Reaction in Stool Detects Transmission of Strongyloides stercoralis from an Infected Donor to Solid Organ Transplant Recipients
Solid organ transplant recipients can acquire Strongyloides
stercoralis from an infected donor. The diagnosis of S.
stercoralis in immunocompromised individuals may be challenging
due to a lower sensitivity of available parasitological and
serological methods, compared with immunocompetent individuals.
Recently, a real-time polymerase chain reaction (RT-PCR) in
stool has been developed for S. stercoralis diagnosis. We report
two cases of S. stercoralis infection transmitted by a donor to
two solid organ transplant recipients, whose stool samples were
diagnosed using RT-PCR. This test could play an important role
in S. stercoralis diagnosis in immunosuppressed patients,
facilitating rapid treatment initiation and reducing the risk of
severe strongyloidiasis. Adherence to current recommendations of
screening among donors and recipients from endemic areas is also
urgently needed
Imported strongyloidiasis in Spain
The objective of this study was to assess the epidemiological, laboratory, and clinical features of imported strongyloidiasis in a tropical medicine referral unit in Madrid, Spain.This was a retrospective study based on a review of medical records. A patient was diagnosed with strongyloidiasis when the infection could be detected by conventional stool analysis and/or serology against Strongyloides stercoralis, regardless of the presence of symptoms. RESULTS: One hundred and seventy-eight cases of strongyloidiasis were included in the study. Stool tests were performed in all patients, and serology in 160 patients (89.9%). The diagnosis of strongyloidiasis was based on serology only in four patients; 21 patients only had positive stool tests. A third of the total strongyloidiasis cases in this study were travel-related, mainly associated with short trips (<2 months). Only 47.8% of total cases were symptomatic. We found no differences in clinical presentation between immigrants and travelers with strongyloidiasis. CONCLUSIONS: Not only should strongyloidiasis be suspected in symptomatic travelers and immigrants, but it should also be ruled out when elevated IgE levels or eosinophilia are present. Strongyloidiasis can be asymptomatic in HIV patients, but it should be diagnosed and treated before a possible hyperinfection develops.This study was supported by RICET RD06/0021/0003 ISCIII-RETICS.
Ethical approval: The study was approved by the institutional ethics committee (GER-STR.201201).S