Historical query data as business intelligence tool on an internationalization contex

[EN] This article reports theory concerning the key strategies of information search behaviour on an international market-orientation context, proposing a common framework to identify search goals using data generated from the keyword planner from google covering the period 2014/17 applied to the clothing retail sector. A conceptual framework of user goal identification from search data within clothing retail sector is presented and discussed in light of existing empirical studies. For that, a case study of an important fashion retailer has been analyzed: Zara. This firm is situated in the second position within the ranking of most valuable brands of clothes 2015 around the world (Kantar, 2016). A comparative analysis of search patters of this company, between United Kingdom and Spain, has been developed in order to offer the possible internationalization strategies in the online retail sector. User goals are identifyed and are stable over the period of study, a framework that covers main clothing consumer search goals have been identifyed.This study has been developed within the Research Project funded by Fundación Ramón Areces, entitled “La colaboración abierta en Internet como estrategia de innovación e internacionalización del sector de moda y complementos [open collaboration in the Internet as innovation and internationalization strategies by fashion sector] (2015-2018).Carro-Rodríguez, J.; Lorenzo-Romero, C.; Gómez-Borja, M. (2018). Historical query data as business intelligence tool on an internationalization contex. En 2nd International Conference on Advanced Reserach Methods and Analytics (CARMA 2018). Editorial Universitat Politècnica de València. 221-228. https://doi.org/10.4995/CARMA2018.2018.8361OCS22122

Control charts based on MATLAB statistical and visualization tools as a compatible with e-learning methodology in the context of quality control

[EN] The advanced tools for statistical process control in the context of management and quality control are very important for students of technology degrees, even more so in the current industry 4.0 context and considering the new strategies and quality philosophies like Six Sigma. This work shows a learning activity compatible with e-learning methodology based on the use of Matlab® to improve the teaching-learning process for quality control based on the generation of different random datasets in matrix form so that they are processed by each student using different statistical and data visualization tools. Three different real situations are addressed based on the nature of the data: one case of a fully statistically controlled process, one case of a process with a violation in the variability, and finally, one case of a process with violation either in the centering and variability. A pilot study with a small group of students is addressed and subsequently the perception of the activity is evaluated using a questionnaire. The activity was perceived by the students as useful and scalableS

La rehabilitación de mujeres delincuentes en la perspectiva de la educación moral

In this article we present a program of pedagogical intervention for the development, among others, of values in women internal in penitentiary centres. It deals with the development of values in imprisoned women from the application of strategies of pedagogical intervention directed to the development of the prosocial thought; these strategies are concentrated on a program of delinquent rehabilitation from the cognitive training, basing itself on a comprehensive model. This program (Prosocial Thought) was put into practice experimentally to a group of women internal in a penitentiary centre in Galicia. The evaluation of the program allows us to assure the efficacy of the same to develop among the inmates the skills and attitudes necessary for their reeducation and social rehabilitationS

The function of the university. From policy strategy to strategic policy

En este artículo examinamos la compleja relación entre política y universidad, dos conceptos con significado propio y que sólo en parte se fusionan respecto de la función de la universidad, que es el objeto principal de este estudio. Una vez delimitado el sentido de la contribución abordamos el marco de la coordinación competencial en nuestro país, junto con las directrices de la educación superior en el marco europeo y el énfasis actual en la búsqueda de la calidad universitaria como vía de desarrollo estratégico. Es justamente la cultura de la evaluación en la educación superior la que favorece un renovado análisis de la calidad en el contexto de una universidad de masas. Acorde con el notable reto derivado del Tratado de Maastricht, nos ocupamos del desarrollo de sistemas universitarios regionales y las políticas estratégicas asociadas a la definición de perfiles en las universidades. Finalmente, referimos algunos elementos importantes para una articulación de funciones que la universidad tendrá que plantearse en el nuevo sigloThe main objective of this article is to examine the complex relationship between policies and the university, two concepts, each with their own meanings that fuse only in part, with respect to the function of the universities themselves. Once the sense of this article has been established, we will analyse the framework given to the co-ordination of what should be done, why and by whom at the different Spanish universities. We will also analyse the guidelines for higher education within the European framework and the current emphasis given to the search for quality in universities, as a means to achieving a strategic development. It is precisely the «culture» of evaluation in higher education that helps towards a renewed analysis of this quality within the context of mass universities. In correlation with the important challenge derived from the Maastricht Treaty, we will deal with the development of regional university systems, as well as the strategic policies related to the definition of university profiles. Finally, we make reference to some important elements for the articulation of functions which universities will have to face in the next millenniumS

Sensory and Physicochemical Analysis of Meat from Bovine Breeds in Different Livestock Production Systems, Pre-Slaughter Handling Conditions, and Ageing Time

Different bovine breeds and production systems are used worldwide, giving rise to differences in intrinsic and extrinsic characteristics of beef. In order to meet the consumer requirements, new approaches are currently being developed to guarantee tenderness, taste, and juiciness of beef. However, the final consumer perception is complex, and it is also affected by several interrelated variables. This study aimed to evaluate the physicochemical parameters and sensory profile of three Spanish cattle breeds under different livestock production systems (extensive and intensive) and pre-slaughter handling conditions (mixing and not mixing with unfamiliar individuals at pre-mortem time). Meat samples from each group were also studied at different ageing times (7 and 14 days). Regarding sensory attributes, twelve panelists assessed meat samples and an exhaustive statistical analysis was carried out. The most evident and strongest effect was the breed type, allowing a great differentiation among them using principal components and discriminant analysis. The livestock production system was the second most important parameter, significantly affecting odor, flavor, and textural profile (fibrousness). It can be concluded that there were marked differences in the traits of these beef that could be modified by other factors in order to fulfill consumer tastesAuthors are grateful to RTA 2014-00034-C04-00 (INIA-MINECO) and FEDER funds for the financial support. Special thanks for financial support from the Xunta de Galicia and the European Union (ESF) for supporting Raquel Rodríguez-Vázquez pre-doctoral scholarship. Daniel Franco and José M. Lorenzo are members of the HealthyMeat network, funded by CYTED (Ref. 119RT0568)S

Kappa-carrageenan as an effective cryoprotectant on water mobility and functional properties of grass carp myofibrillar protein gel during frozen storage

The effect of kappa-carrageenan oligosaccharides was analyzed on the gelling abilities of grass carp myofibrillar proteins (MP) during 60 days frozen storage. Four gel treatments were prepared: control without additives (C), a positive control with sucrose: sorbitol (PC), and two batches with 1 and 2% of kappa-carrageenan (KC). All MP gels showed a significant decline in the functional and structural properties. Water holding capacity (WHC) and textural properties of control MP gel was significantly reduced due to oxidative changes, whereas weak cross-linking between amino acid molecules and denaturation of myosin observed. In contrast, PC and KC showed a significant control on the reduction of functional properties due to the enhanced inter/intra-molecular interactions and less formation of ice crystals. Addition of KC (2%) significantly restricted the decline in WHC from 95.45 to 75.47%, which is well associated with high hydrogen proton density. Besides that, low field nuclear magnetic resonance (LF-NMR) analysis exhibited a restricted increase in T22 relaxation time in samples added with KC (2%). Overall, MP gel with KC (2%) proved to be an effective alternative in comparison with PC and could be efficient in the production and commercialization of fish and other seafood products.National Natural Science Foundation of China | Ref. 31972104National Natural Science Foundation of China | Ref. 51809224National Natural Science Foundation of China | Ref. 3147169

Structural organization of mammalian prions as probed by limited proteolysis

Elucidation of the structure of PrP(Sc) continues to be one major challenge in prion research. The mechanism of propagation of these infectious agents will not be understood until their structure is solved. Given that high resolution techniques such as NMR or X-ray crystallography cannot be used, a number of lower resolution analytical approaches have been attempted. Thus, limited proteolysis has been successfully used to pinpoint flexible regions within prion multimers (PrP(Sc)). However, the presence of covalently attached sugar antennae and glycosylphosphatidylinositol (GPI) moieties makes mass spectrometry-based analysis impractical. In order to surmount these difficulties we analyzed PrP(Sc) from transgenic mice expressing prion protein (PrP) lacking the GPI membrane anchor. Such animals produce prions that are devoid of the GPI anchor and sugar antennae, and, thereby, permit the detection and location of flexible, proteinase K (PK) susceptible regions by Western blot and mass spectrometry-based analysis. GPI-less PrP(Sc) samples were digested with PK. PK-resistant peptides were identified, and found to correspond to molecules cleaved at positions 81, 85, 89, 116, 118, 133, 134, 141, 152, 153, 162, 169 and 179. The first 10 peptides (to position 153), match very well with PK cleavage sites we previously identified in wild type PrP(Sc). These results reinforce the hypothesis that the structure of PrP(Sc) consists of a series of highly PK-resistant β-sheet strands connected by short flexible PK-sensitive loops and turns. A sizeable C-terminal stretch of PrP(Sc) is highly resistant to PK and therefore perhaps also contains β-sheet secondary structure

Bioactives Overproduction through Operational Strategies in the Ichthyotoxic Microalga Heterosigma akashiwo Culture

The red tide-forming microalga Heterosigma akashiwo has been associated with massive events of fish deaths, both wild and cultured. Culture conditions are responsible for the synthesis or accumulation of some metabolites with different interesting bioactivities. H. akashiwo LC269919 strain was grown in a 10 L bubble column photobioreactor artificially illuminated with multi-coloured LED lights. Growth and production of exopolysaccharides, polyunsaturated fatty acids (PUFAs), and carotenoids were evaluated under different culture modes (batch, fed-batch, semicontinuous, and continuous) at two irradiance levels (300 and 700 µE·s−1·m−2). Continuous mode at the dilution rate of 0.2·day−1 and 700 µE·s−1·m−2 provided the highest production of biomass, PUFAs (132.6 and 2.3 mg·L−1·day−1), and maximum fucoxanthin productivity (0.16 mg·L−1·day−1). The fed-batch mode accumulated exopolysaccharides in a concentration (1.02 g·L−1) 10-fold over the batch mode. An extraction process based on a sequential gradient partition with water and four water-immiscible organic solvents allowed the isolation of bioactive fucoxanthin from methanolic extracts of H. akashiwo. Metabolites present in H. akashiwo, fucoxanthin and polar lipids (i.e., eicosapentaenoic acid (EPA)), or probably such as phytosterol (β-Sitosterol) from other microalgae, were responsible for the antitumor activity obtained.This research was funded by the General Secretariat of Universities, Research and Technology of the Andalusian Government (grant: P18-RT-2477) and the State Research Agency (grants PID2019-109476RB-C22) of the Spanish Ministry of Science, Innovation and Universities

Structure of the unusual Sinorhizobium fredii HH103 lipopolysaccharide and its role in symbiosis

Rhizobia are soil bacteria that form important symbiotic associations with legumes, and rhizobial surface polysaccharides, such as K-antigen polysaccharide (KPS) and lipopolysaccharide (LPS), might be important for symbiosis. Previously, we obtained a mutant of Sinorhizobium fredii HH103, rkpA, that does not produce KPS, a homopolysaccharide of a pseudaminic acid derivative, but whose LPS electrophoretic profile was indistinguishable from that of the WT strain. We also previously demonstrated that the HH103 rkpLMNOPQ operon is responsible for 5-acetamido-3,5,7,9-tetradeoxy-7-(3-hydroxybutyramido)-L-glyc-ero-L-manno-nonulosonic acid [Pse5NAc7(3OHBu)] production and is involved in HH103 KPS and LPS biosynthesis and that an HH103 rkpM mutant cannot produce KPS and displays an altered LPS structure. Here, we analyzed the LPS structure of HH103 rkpA, focusing on the carbohydrate portion, and found that it contains a highly heterogeneous lipid A and a peculiar core oligosaccharide composed of an unusually high number of hexuronic acids containing b-configured Pse5NAc7(3OHBu). This pseudaminic acid derivative, in its a-configuration, was the only structural component of the S. fredii HH103 KPS and, to the best of our knowledge, has never been reported from any other rhizobial LPS. We also show that Pse5NAc7(3OHBu) is the complete or partial epitope for a mAb, NB6-228.22, that can recognize the HH103 LPS, but not those of most of the S. fredii strains tested here. We also show that the LPS from HH103 rkpM is identical to that of HH103 rkpA but devoid of any Pse5NAc7(3OHBu) residues. Notably, this rkpM mutant was severely impaired in symbiosis with its host, Macroptilium atropurpureum.Fil: Di Lorenzo, Flaviana. Università degli Studi di Napoli Federico II; ItaliaFil: Speciale, Immacolata. Università degli Studi di Napoli Federico II; ItaliaFil: Silipo, Alba. Università degli Studi di Napoli Federico II; ItaliaFil: Alías Villegas, Cynthia. Universidad de Sevilla; EspañaFil: Acosta Jurado, Sebastián. Universidad de Sevilla; EspañaFil: Rodríguez Carvajal, Miguel Ángel. Universidad de Sevilla; EspañaFil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Palmigiano, Angelo. Consiglio Nazionale delle Ricerche; ItaliaFil: Garozzo, Domenico. Consiglio Nazionale delle Ricerche; ItaliaFil: Ruiz Sainz, José Enrique. Universidad de Sevilla; EspañaFil: Molinaro, Antonio. University of Naples Federico II; ItaliaFil: Vinardell, José María. Universidad de Sevilla; Españ