Genome comparison and context analysis reveals putative mobile forms of restriction–modification systems and related rearrangements

The mobility of restriction–modification (RM) gene complexes and their association with genome rearrangements is a subject of active investigation. Here we conducted systematic genome comparisons and genome context analysis on fully sequenced prokaryotic genomes to detect RM-linked genome rearrangements. RM genes were frequently found to be linked to mobility-related genes such as integrase and transposase homologs. They were flanked by direct and inverted repeats at a significantly high frequency. Insertion by long target duplication was observed for I, II, III and IV restriction types. We found several RM genes flanked by long inverted repeats, some of which had apparently inserted into a genome with a short target duplication. In some cases, only a portion of an apparently complete RM system was flanked by inverted repeats. We also found a unit composed of RM genes and an integrase homolog that integrated into a tRNA gene. An allelic substitution of a Type III system with a linked Type I and IV system pair, and allelic diversity in the putative target recognition domain of Type IIG systems were observed. This study revealed the possible mobility of all types of RM systems, and the diversity in their mobility-related organization

Rapid Synthesis of L-Idosyl Glycosyl Donors from α- Thioglucosides for the Preparation of Heparin Disaccharides

A new methodology for the synthesis of the most challenging heparin building block has been developed. Orthogonally protected L-idosyl glycosyl donors were prepared by C5 epimerization of the corresponding thioglucosides using the hydroboration/oxidation method followed by a 4,6-acetal formation. The -anomeric configuration was crucial and the bulky C4 substituent was advantageous for the high L-ido diastereoselectivity. The 4,6-arylmethylene group proved to be a directing element in glycosylation thereby stereoselective -idosylation could be achieved by using idosyl donors without a C-2 participating group

Focus - Montage et réalisation de projets de recherche entre organisme de recherche, gestionnaires et bureau d’étude : analyse à travers l’expérience d’Asconit Consultants

Une collaboration active entre organisme de recherche, gestionnaires et bureau d'étude contribue à donner plus d’ampleur à un projet de recherche et développement et peut accélérer l’innovation, qu'elle soit technologique ou méthodologique. Au travers de l’expérience acquise depuis une quinzaine d’années par Asconit Consultants, les facteurs-clé du succès des projets sont analysés, les limites identifiées, ainsi que les voies d'amélioration

Hémophilie A acquise (étude rétrospective de 1993 à 2010 au CHU de Nantes)

L'hémophilie A acquise (HA) est une maladie hémorragique acquise rare par auto-anticorps anti-FVIII, idiopathique ou associée à une autre pathologie (post-partum, néoplasie, maladie auto-immune). Le traitement consiste en la prise en charge du syndrome hémorragique, et en l'éradication de l inhibiteur par un traitement immunosuppresseur. Nous rapportons une série de 36 patients consécutifs pris en charge au CHU de Nantes entre 1993 et 2010 pour HA. Ce travail rétrospectif mono-centrique retrouve des données conformes à celles de la littérature. Il permet de ré-insister sur la nécessité d'un diagnostic et d'un traitement précoces, ce qui nécessite une sensibilisation des médecins à cette maladie. Il insiste sur la iatrogénie très importante du traitement de fond, et sur la nécessité d'évaluer la place du rituximab. Il permet de réfléchir à l'établissement d un score de gravité pour guider ce traitement. Il met également en avant l'intérêt du monitoring du ratio FVIII:C/VWF:Ag dans le dépistage et la prise en charge optimale des rechutes.NANTES-BU Médecine pharmacie (441092101) / SudocSudocFranceF

2-years rotation, an alternative to maize monocropping in Bearn, France (DiverIMPACTS Practice Abstract)

Thanks to the introduction of legumes and cover crops, which enable crop diversification whilst qualifying for coupled aids, this innovative system has proved to enhance agronomic, environmental and economic performances. On a 3 years average basis, the 2-years rotation has proven to release 32% less GHG than maize monocropping. This is mainly due to a significant decrease of operating costs enabled by lower nitrogen inputs (-40%) thanks to the soybeans. It also brings economic satisfaction as soybean is well adapted in this region and a biogas plant gives additional added value to the energy catch crop. Even with higher mechanisation costs due to the ECC, the net margin with aid remains higher than that of the reference monocropping (+ 37€/hectare on average over 3 years, although similar margin per hour worked)

Introducing oats as an energy catch crop in maize monocropping in Bearn, France (DiverIMPACTS Practice Abstract)

This tool informs about introducing oats as an energy catch crop in maize monocropping in Bearn, France, mentiones the benefits and gives practical recommendations