Generation of an inducible expression system to perform molecular pharmacology studies with mu opioid receptors

Máster en Biología Molecular y Biomedicin

Adaptive behaviour and paddle tennis: a case study of Down’s syndrome

Adaptive behaviour is one of the key elements to diagnose intellectual disability. In addition, these be-haviors are crucial in order to operate on the daily life. As a population with a high risk of developing a sedentary lifestyle, adapted physical activity programs for people and children with intellectual disability are needed. Therefore the aim of this study is to assess the influence of adapted paddle tennis intervention program on the adaptive behaviour of a child with Down’s syndrome. In a case study of child with Down’s syndrome (male, Caucasian, age = 4.5 years), he participated on a paddle tennis program with a length of 12 weeks. The child’s parents assessed the adaptive behaviour before and after the program through the Adapted Behaviour Scale (ABS-S:2). Obtained data showed that three domains presented a positive increasing. Parents reported that physical development increased from 19 to 23.5, and this in-crease was corroborated by the instructor (post score = 23.8). Moreover, language development increases from 18.5 to 28 (parents). The paddle tennis instructor perceived an increase in speaking abilities after the program. The self-management domain also showed an increase from 8 to 17. The adapted paddle tennis program is suitable for promoting physical activity and for improving adaptive behaviour in children with intellectual disabilities.This research has been subsidized by the Fundación Repsol, through the "Adaptive Behaviour and a Paddle Tennis: a case study of Down´s syndrome" project (Fundación Deporte Joven, Consejo Superior de Deportes (CSD), Asociación Padelparatodos (ASPADO), UAM

La resiliencia en entrenadores de pádel adaptado

En el Deporte, la mayoría de los estudios sobre resiliencia se han centrado en deportistas, siendo reducido el número de investigaciones con entrenadores, sobre todo en el ámbito del deporte adaptado. Por ello, los objetivos del trabajo son: describir las características de resiliencia de entrenadores de Pádel Adaptado desarrollando baremos específicos para muestra analizada; establecer posibles diferencias en los niveles de resiliencia considerando variables personales y deportivas; establecer posibles relaciones entre los niveles de resiliencia, la edad y experiencia; comparar los niveles de resiliencia de la presente muestra con los resultados de estudios precedentes y determinar los niveles de fiabilidad obtenidos con la escala de resiliencia utilizada. La muestra estaba integrada por 111 entrenadores de pádel adaptado, a los que se aplicó dos instrumentos: cuestionario sociodemográfico para entrenadores de Pádel Adaptado (Ruiz, 2004; RuizBarquín, De la Vega, De la Rocha y Batista, 2015a) y la adaptación al castellano del cuestionario de Resiliencia (Ruiz, De la Vega, Poveda, Rosado y Serpa, 2012). Los resultados señalan un alto porcentaje de entrenadores con alta resiliencia (35.10%), mostrando niveles superiores a anteriores estudios y adecuados niveles de fiabilidad. La ausencia de diferencias significativas considerando las variables personales y deportivas, y las implicaciones de prácticas derivadas de los resultados obtenidos, son discutidas. Palabras clave: Resiliencia; entrenadores; pádel; deporte adaptadoIn Sport, most research on resilience is focused on athletes, with quite a low amount on coaches, especially regarding adapted sport. Therefore, the aims of this research are: to describe the resilience characteristics of Adapted paddle coaches by developing specific scales for the sample under study; to establish possible differences in the total resilience levels considering different personal and sport variables; to establish possible relationships between the resilience levels of Adapted Paddle Coaches and the age and experience variables; to compare the resilience levels within the current sample with results in previous studies; and to evaluate reliability levels obtained with the resilience scale used. The sample comprised 111 adapted paddle coaches, to whom two questionnaires were given: a sociodemographic one for adapted paddle coaches (Ruiz, 2004; Ruiz-Barquín, De la Vega, De la Rocha y Batista, 2015a) and the Spanish version of resilience (Ruiz, De la Vega, Poveda, Rosado & Serpa, 2012). The results conclude that the sample group has a large percentage of coaches with high resilience (35.10%), showing higher levels than those in previous studies, and appropriate reliability levels. The absence of significant differences, taking into account the personal and sport variables, and the implications of practices deriving from the results obtained, are discussed in the articl

Atención de Enfermería brindada al recién nacido, Sala de Labor y Parto, Hospital San José–Diriamba–Carazo, I–II Semestre 2015

Atención de Enfermería brindada al recién nacido, Sala de labor y parto Hospital San José Diriamba-Carazo I Semestre 2015. Según la OMS, (2000) Todo personal de salud garantizará la atención adecuada y completa a todo recién nacido, de acuerdo a cada una de sus necesidades, priorizando su estado completo de salud por lo tanto cada personal de atención debe de cumplir estrictamente con las técnicas necesarias y tener el conocimiento adecuado. La presente investigación se realizó en el Hospital San José Diriamba-Carazo en la Sala de labor y parto, al personal de enfermería que brinda los cuidados al recién nacido, tiene un tipo de estudio prospectivo, de corte transversal, descriptivo, cuantitativo, contiene universo, utilizando los instrumentos para la recopilación de la información, guía de observación y revisión del expediente clínico. Se concluyó que en la sala de labor y parto se brindó la atención de enfermería al recién nacido, encontrándose en el análisis de los resultados un 100% de la antigüedad laboral se encuentran las enfermeras materno infantil y ciencias de la enfermería. En los cuidados de la atención inmediatas encontrándose en un 100% el secado, valoración Apgar y pinzamiento del cordón, por lo tanto en la atención mediata se cumplieron su mayoría en un 100% de igual manera en la atención ayudando a bebés a respirar se cumplió en un 100% excepto el lavado de mano en un 78% y en la medida de prevención de infecciones se cumplieron un 100% excepto un recién nacido por cuna en un 96%. Por lo tanto se recomienda a las autoridades de la institución que se brinden capacitaciones, monitoreo al personal de enfermería sobre la atención al recién nacido de igual manera recomendar que se registren en el expediente clínico de manera correcta con cual se evidenciará el trabajo realizado

Role of SOX family of transcription factors in central nervous system tumors

SOX genes are developmental regulators with functions in the instruction of cell fate and maintenance of progenitor’s identity during embryogenesis. They play additional roles during tissue homeostasis and regeneration in adults particularly in the Central Nervous System (CNS). In the last years a growing number of evidences has shown that mutations and dysfunction of SOX factors are implicated in several human diseases, including a variety of cancers. In this review, we will summarize the current knowledge about SOX family in CNS tumors and their role in the origin and maintenance of the subpopulation of cancer stem cells in these tumors

Una revisión sobre la consolidación empresarial en España

La continua y veloz evolución que están experimentando los mercados actuales de todo el mundo, han propiciado la concentración de numerosas empresas con la finalidad de dar respuesta a las estrategias de crecimiento y la mejora de desarrollo que esta nueva situación conlleva. La importancia del fenómeno de concentración empresarial en España en los últimos años, justifica la necesidad de realizar un nuevo estudio sobre la situación actual española. El presente trabajo toma como unidad de análisis el total de grupos que cotizan en la actualidad en la Bolsa española, tratando de mostrar su realidad. De este modo, permite conocer la estabilidad que tienen dentro del mercado español y, al mismo tiempo, aproximamos al método de consolidación empleado, las características de las sociedades dependientes y otros rasgos claves que faciliten un mayor conocimiento de tales entidades

Potentiation of morphine-induced antinociception and locomotion by citalopram is accompanied by anxiolytic-like effects

Morphine and related opioids are the mainstay of analgesic treatment, especially in patients suffering chronic pain. Besides their antinociceptive effects they may also exhibit anxiolytic-like properties that could contribute to pain relief. The pharmacological manipulation of the serotonergic system may not only modulate pain transmission and processing but also other behavioral effects of opioids. The present study aimed to analyze the effect of the concurrent treatment with citalopram, a selective serotonin reuptake inhibitor, on the antinociceptive, locomotor and anxiety-related effects induced by acute and subchronic administration of morphine in mice. Citalopram (15 mg/kg) enhanced the acute antinociceptive effects of morphine when concurrently administered as evidenced by a two-fold increase in the ED50 for the antinociceptive effect of morphine in the hot-plate test. Chronic studies also revealed that concurrent citalopram treatment (15 mg/kg) delayed the development of tolerance to the thermal antinociceptive effects of morphine. Additionally, morphine-induced hyperlocomotion was potentiated by citalopram as assessed in the open-field test and in the spontaneous activity recording in the home cage, a behavioral outcome to which tolerance or desensitization was not developed. Interestingly, chronic administration of both drugs promoted an anxiolytic effect as evidenced by the increased central activity in the open field test. Future investigations on this pharmacological interaction, such as the possible translational research in clinics, might have consequences in future strategies for the therapeutic management of pain.This research was supported by the grant SAF2010-15663 from the Spanish Government (MINECO). We thank David Ramos and Raquel Lanza for their technical assistance in handling living animals. We kindly appreciate Belen Palacio support for video footage and editing and Dr. Albert Adell for the critical reading of the manuscript

Comparación del acompañamiento presencial contra en línea y su relación con el desarrollo infantil en la primera infancia

A quasi-experimental analysis is performed through the analysis of variance and multivariate analysis of variance methodology. The null hypothesis establishes that the variables of age, sex and difference in online vs. face-to-face support do not influence the results obtained in the development tests in which children participated in during their early childhood. The variables were selected based on what was established by Bowlby (1969) where our findings show that the difference between the online vs. the face-to-face program influenced the results obtained in the SDQ: CAS test.Se realiza un análisis cuasi-experimental a través de la metodología del análisis de varianza y multivariantes de varianza. La hipótesis nula establece que las variables de edad, sexo y diferencia de acompañamiento online vs. presencial no influyen en los resultados obtenidos en las pruebas de desarrollo de quienes se encuentran en su primera infancia. Las variables fueron seleccionadas a partir de lo establecido por Bowlby (1969). Se encontró que la diferencia de acompañamiento online vs. presencial influye en los resultados obtenidos en la prueba SDQ: CAS

Key components of the eight classes of type IV secretion systems involved in bacterial conjugation or protein secretion

Conjugation of DNA through a type IV secretion system (T4SS) drives horizontal gene transfer. Yet little is known on the diversity of these nanomachines. We previously found that T4SS can be divided in eight classes based on the phylogeny of the only ubiquitous protein of T4SS (VirB4). Here, we use an ab initio approach to identify protein families systematically and specifically associated with VirB4 in each class. We built profiles for these proteins and used them to scan 2262 genomes for the presence of T4SS. Our analysis led to the identification of thousands of occurrences of 116 protein families for a total of 1623 T4SS. Importantly, we could identify almost always in our profiles the essential genes of well-studied T4SS. This allowed us to build a database with the largest number of T4SS described to date. Using profile-profile alignments, we reveal many new cases of homology between components of distant classes of T4SS. We mapped these similarities on the T4SS phylogenetic tree and thus obtained the patterns of acquisition and loss of these protein families in the history of T4SS. The identification of the key VirB4-associated proteins paves the way toward experimental analysis of poorly characterized T4SS classes.Funding. Spanish Ministry of Economy [BFU2011-26608]; European Seventh Framework Program [282004/FP7-HEALTH.2011, 612146/FP7-ICT-2013]; European Research Council Grant [EVOMOBILOME no. 281605]. Source of open access funding: European Research Council grant to the PI