Hsa-miR-139-5p is a prognostic thyroid cancer marker involved in HNRNPF-mediated alternative splicing.

It is critical to identify biomarkers and functional networks associated with aggressive thyroid cancer to anticipate diseaseprogression and facilitate personalized patient management. We performed miRNome sequencing of 46 thyroid tumorsenriched with advanced disease patients with a median follow-up of 96 months. MiRNome profiles correlated with tumor-specific histopathological and molecular features, such as stromal cell infiltration and tumor driver mutation. Differentialexpression analysis revealed a consistent hsa-miR-139-5p downexpression in primary carcinomas from patients withrecurrent/metastatic disease compared to disease-free patients, sustained in paired local metastases and validated in publiclypost-print1,62 M

Multiclassifier Systems for Predicting Neurological Outcome of Patients with Severe Trauma and Polytrauma in Intensive Care Units

[EN]This paper presents an ensemble based classification proposal for predicting neurological outcome of severely traumatized patients. The study comprises both the whole group of patients and a subgroup containing those patients suffering traumatic brain injury (TBI). Data was gathered from patients hospitalized in the Intensive Care Unit (ICU) of the University Hospital in Salamanca. Predictive models were induced from both epidemiologic and clinical variables taken at the emergency room and along the stay in the ICU. The large number of variables leads to a low accuracy in the classifiers even when feature selection methods are used. In addition, the presence of a much larger number of instances of one of the classes in the subgroup of TBI patients produces a significantly lesser precision for the minority class

Procrastinación académica y estrés académico en estudiantes universitarios violentados de Piura, 2022

La presente investigación tuvo como finalidad determinar la relación entre la procrastinación académica y el estrés académico en estudiantes universitarios violentados. Para esto la investigación se realizó bajo un enfoque cuantitativo, con un diseño no experimental, transversal, de alcance correlacional, aplicando instrumentos debidamente validados a una muestra de 377 estudiantes de una universidad privada de Piura. En conclusión, se determinó la asociación entre la procrastinación académica y el estrés académico en estudiantes universitarios violentados de Piura, encontrándose que sí existe relación entre ambas variables con un grado de correlación del 23% (0.227) y un nivel de significancia estadística de 0.000 (< 0.05)

Impact of Extrinsic and Intrinsic Hypoxia on Catecholamine Biosynthesis in Absence or Presence of Hif2α in Pheochromocytoma Cells

Pheochromocytomas and paragangliomas (PPGLs) with activated pseudohypoxic pathways are associated with an immature catecholamine phenotype and carry a higher risk for metastasis. For improved understanding of the underlying mechanisms we investigated the impact of hypoxia and pseudohypoxia on catecholamine biosynthesis in pheochromocytoma cells naturally lacking Hif2α (MPC and MTT) or expressing both Hif1α and Hif2α (PC12). Cultivation under extrinsic hypoxia or in spheroid culture (intrinsic hypoxia) increased cellular dopamine and norepinephrine contents in all cell lines. To distinguish further between Hif1α- and Hif2α-driven effects we expressed Hif2α in MTT and MPC-mCherry cells (naturally lacking Hif2α). Presence of Hif2α resulted in similarly increased cellular dopamine and norepinephrine under hypoxia as in the control cells. Furthermore, hypoxia resulted in enhanced phosphorylation of tyrosine hydroxylase (TH). A specific knockdown of Hif1α in PC12 diminished these effects. Pseudohypoxic conditions, simulated by expression of Hif2α under normoxia resulted in increased TH phosphorylation, further stimulated by extrinsic hypoxia. Correlations with PPGL tissue data led us to conclude that catecholamine biosynthesis under hypoxia is mainly mediated through increased phosphorylation of TH, regulated as a short-term response (24-48 h) by HIF1α. Continuous activation of hypoxia-related genes under pseudohypoxia leads to a HIF2α-mediated phosphorylation of TH (permanent status).Funding: This research was funded by the Deutsche Forschungsgemeinschaft (DFG) within the CRC/Transregio205/1 (project number: 314061271-TRR 205), Project No. B12 (N.B. and G.E.), Project No. B10 (S.R., J.P. and M.U.)and Project No. S01 (A.W., C.G. and M.P.) “The Adrenal: Central Relay in Health and Disease“, and by theParadi erence Foundation (N.B., I.P., S.R. and G.E.).S

El papel del farmacéutico en la cistitis

La infección del tracto urinario (ITU), con sus múltiples presentaciones clínicas, está entre las enfermedades infecciosas más frecuentes, tanto en pacientes ambulatorios como en pacientes hospitalizados. Esta patología suele afectar más a las mujeres debido a sus condiciones anatómicas y hormonales. Existen diversas estrategias para la prevención de estas infecciones, en las cuales jugará un papel muy importante el consejo farmacéutico en la farmacia comunitaria. En cuanto al tratamiento, existen distintos cuadros clínicos que requerirán diferentes duraciones de terapia antibiótica. Tanto el exceso como el defecto deberán evitarse. A lo largo de este trabajo hablaremos de los síntomas, antecedentes, prevención y tratamiento de estas infecciones, centrándonos sobre todo en la cistitis recurrente y en el uso de las plantas medicinales para su profilaxis

Missed clinical clues in patients with pheochromocytoma/paraganglioma discovered by imaging

CONTEXT: Pheochromocytomas and paragangliomas (PPGLs) are rare but potentially harmful tumors that can vary in their clinical presentation. Tumors may be found due to signs and symptoms, as part of a hereditary syndrome or following an imaging procedure. OBJECTIVE: To investigate potential differences in clinical presentation between PPGLs discovered by imaging (iPPGLs), symptomatic cases (sPPGLs) and those diagnosed during follow-up because of earlier disease/known hereditary mutations (fPPGL). DESIGN: Prospective study protocol, which has enrolled patients from 6 European centers with confirmed PPGLs. SETTING AND PATIENTS: Data were analyzed from 235 patients (37% iPPGLs, 36% sPPGLs, 27% fPPGLs) and compared for tumor volume, biochemical profile, mutation status, presence of metastases and self-reported symptoms. RESULTS: iPPGL patients were diagnosed at a significantly higher age than fPPGLs (p<0.001), found to have larger tumors (p=0.003) and higher metanephrine and normetanephrine levels at diagnosis (p=0.021). Significantly lower than in sPPGL, there was a relevant number of self-reported symptoms in iPPGL (2.9 vs. 4.3 symptoms, p<0.001). In 16.2% of iPPGL, mutations in susceptibility genes were detected, although this proportion was lower than in fPPGL (60.9%) and sPPGL (21.5%). CONCLUSIONS: Patients with PPGLs detected by imaging were older, have higher tumor volume and more excessive hormonal secretion in comparison to those found as part of a surveillance program. Presence of typical symptoms indicates that in a relevant proportion of those patients the PPGL diagnosis had been delayed. Précis: Pheochromocytoma/paraganglioma discovered by imaging are often symptomatic and carry a significant proportion of germline mutations in susceptibility genes.The research leading to these results has received funding from the following sources: The Seventh Framework Programme (FP7/2007–2013) under grant agreement n° 259735 awarded to F B, H T and G E. The study has further been supported by the Deutsche Forschungsgemeinschaft (DFG) within the CRC/Transregio 205/1 ‘The Adrenal: Central Relay in Health and Disease’ to M F, M R, J L, G E, and F B. The authors are grateful to all patients who participated in this research and to Christina Brugger, Katharina Langton and Denise Kaden for excellent technical assistance.S

Planeamiento estratégico para la Industria peruana de la madera y derivados

La industria de la Madera y sus Derivados conoce un gran crecimiento a nivel mundial. La madera, a diferencia de muchos otros recursos naturales, es un producto ecológico, renovable, y utilizado de manera adecuada, posee características de calidad que sobrepasan a las de sus principales sustitutos. Por ello, este sector es uno de los únicos con una proyección de desarrollo a nivel global garantizada por los próximos 50 años. En el Perú sin embargo, la industria de la madera y sus derivados no ha recibido la atención necesaria, ya sea por parte del sector público o del sector privado. A pesar de que nuestro país está catalogado como uno de los nueve países con mayor potencial forestal en el mundo, casi todos nuestros vecinos a nivel sudamericano nos superan tanto en capacidad productiva como en ingresos generados gracias a las exportaciones. La tala ilegal, la falta de inversión en investigación y desarrollo, la poca rentabilidad por hectárea y sobretodo la falta de un Plan Estratégico para el sector, han permitido que nos encontremos actualmente con una industria con poco crecimiento y que representa menos del 0.5% de nuestro PBI. Este documento propone el Plan Estratégico de la Industria de la Madera y sus Derivados, cuyo objetivo es el desarrollo del sector, alcanzando la visión trazada hacia el horizonte 2027 con un importante nivel de ventas, rentabilidad atractiva, generación de empleo estable y bien remunerado, y una alta competitividad. Una vez definida la Visión, se fijaron cuatro objetivos a largo plazo necesarios para su alcance, quienes a su vez se sustentan en 17 objetivos a corto plazo. Todo ello será posible luego de implementar nueve estrategias, resultantes de las matrices estudiadas, basadas principalmente en el aumento de plantaciones forestales, mejora de la calidad de nuestra madera, protección de los recursos naturales y búsqueda de nuevos mercadosWood and wood-based products Industry is expected to have a significant growth worldwide. Unlike other types of natural resources, timber is an ecological, renewable product and, used properly, possesses special quality characteristics that exceed its substitute products. For this reason, Wood industry is almost the only resource sector to have a positive growth projection guaranteed for the next fifteen years. In Peru nevertheless, Timber Industry and its byproducts have not been receiving proper attention from public and private sectors. Despite being catalogued amongst the top nine countries in the world in forestry potential, practically all neighboring countries in South America have overcome our country in production capacities as well as in export generated income. Nowadays, our industry lacks growing perspectives and represents less than 0.5% of Peruvian Gross Domestic Product. This is mainly due to extensive illegal logging, lack of research and development investment, low operation yields and the absence of a general strategic plan for the industry. With that in mind, this document sponsors a complete Wood and wood-based products Industry Strategic Plan, whose purpose is to develop this sector and achieve our 2027 vision. This vision includes a large level of sales, both domestic and international, high profitability and well remunerated stable jobs. The aim of this Strategic Plan is to maximize the natural potential of Peru, based on defined strategies, short-term and long term objectives, so as our industry will thrive at the forefront in the Latin American region as well as globallyTesi

Recensiones [Revista de Historia Económica Año XVII Primavera-Verano 1999 n. 2 pp. 465-494]

Editada en la Fundación Empresa PúblicaE. Torres Villanueva. Ramón de la Sota, 1857-1936. Un empresario vasco (Por Mercedes Cabrera).-- S. López García y J. M. Valdaliso (eds). ¿Que inventen ellos? Tecnología, empresa y cambio económico en la España contemporánea (Por José M.Ortiz-Villajes).-- J. M. Zaratiegui. Caballeros y empresarios. El hombre de negocios Victoriano (Por Juan Manuel Mates Barco).-- M. Arroyo Huguet. La industria del gas en Barcelona, 1841-1933 (Por Carlos Larrinaga Rodríguez).-- M. Seoane. El burgués maldito. La historia secreta de José Ber Gelbard (Por Raúl García Heras).-- J. A. Miranda Encarnación. La industria del calzado en España (1860-1959) (Por Mar Cebrián).-- J. M. Valdaliso. La navegación regular de cabotaje en España en los siglos XIX y XX (Por Richard Sicotte).-- J. R. Modesto Alapont. A ús i costum de bon llamador L'arrendament de terres a l'Horta de Valencia, 1780-1860 (Por Ricardo Robledo).-- I. Iriarte Goñi. Bienes comunales y capitalismo agrario en Navarra, 1855-1935 (Por Antonio Miguel Linares).-- T. Tortella. Los primeros billetes españoles: las «cédulas» del Banco de San Carlos (1782-1829) (Por Pablo Martín Aceña)Publicad

Evaluation of a functional epigenetic approach to identify promoter region methylation in phaeochromocytoma and neuroblastoma

The molecular genetics of inherited phaeochromocytoma have received considerable attention, but the somatic genetic and epigenetic events that characterise tumourigenesis in sporadic phaeochromocytomas are less well defined. Previously, we found considerable overlap between patterns of promoter region tumour suppressor gene (TSG) hypermethylation in two neural crest tumours, neuroblastoma and phaeochromocytoma. In order to identify candidate biomarkers and epigenetically inactivated TSGs in phaeochromocytoma and neuroblastoma, we characterised changes in gene expression in three neuroblastoma cell lines after treatment with the demethylating agent 5-azacytidine. Promoter region methylation status was then determined for 28 genes that demonstrated increased expression after demethylation. Three genes HSP47, homeobox A9 (HOXA9) and opioid binding protein (OPCML) were methylated in >10% of phaeochromocytomas (52, 17 and 12% respectively). Two of the genes, epithelial membrane protein 3 (EMP3) and HSP47, demonstrated significantly more frequent methylation in neuroblastoma than phaeochromocytoma. These findings extend epigenotype of phaeochromocytoma and identify candidate genes implicated in sporadic phaeochromocytoma tumourigenesis