A microsatellite marker for yellow rust resistance in wheat

Bulk segregant analysis (BSA) was used to identify molecular markers associated with yellow rust disease resistance in wheat (Triticum aestivum L.). DNAs isolated from the selected yellow rust tolerant and susceptible F-2 individuals derived from a cross between yellow rust resistant and susceptible wheat genotypes were used to established a "tolerant" and a "susceptible" DNA pool. The BSA was then performed on these DNA pools using 230 markers that were previously mapped onto the individual wheat chromosomes. One of the SSR markers (Xgwm382) located on chromosome group 2 (A, B, D genomes) was present in the resistant parent and the resistant bulk but not in the susceptible parent and the susceptible bulk, suggesting that this marker is linked to a yellow rust resistance gene. The presence of Xgwm382 was also tested in 108 additional wheat genotypes differing in yellow rust resistance. This analysis showed that 81% of the wheat genotypes known to be yellow rust resistant had the Xgwm382 marker, further suggesting that the presence of this marker correlates with yellow rust resistance in diverse wheat germplasm. Therefore, Xgwm382 could be useful for marker assisted selection of yellow rust resistances genotypes in wheat breeding programs

Uniqueness Theorem for Generalized Maxwell Electric and Magnetic Black Holes in Higher Dimensions

Based on the conformal energy theorem we prove the uniqueness theorem for static higher dimensional electrically and magnetically charged black holes being the solution of Einstein (n-2)-gauge forms equations of motion. Black hole spacetime contains an asymptotically flat spacelike hypersurface with compact interior and non-degenerate components of the event horizon.Comment: 7 pages, RevTex, to be published in Phys.Rev.D1

Spatial infinity in higher dimensional spacetimes

Motivated by recent studies on the uniqueness or non-uniqueness of higher dimensional black hole spacetime, we investigate the asymptotic structure of spatial infinity in n-dimensional spacetimes($n \geq 4$). It turns out that the geometry of spatial infinity does not have maximal symmetry due to the non-trivial Weyl tensor {}^{(n-1)}C_{abcd} in general. We also address static spacetime and its multipole moments P_{a_1 a_2 ... a_s}. Contrasting with four dimensions, we stress that the local structure of spacetimes cannot be unique under fixed a multipole moments in static vacuum spacetimes. For example, we will consider the generalized Schwarzschild spacetimes which are deformed black hole spacetimes with the same multipole moments as spherical Schwarzschild black holes. To specify the local structure of static vacuum solution we need some additional information, at least, the Weyl tensor {}^{(n-2)}C_{abcd} at spatial infinity.Comment: 6 pages, accepted for publication in Physical Review D, published versio

On the `Stationary Implies Axisymmetric' Theorem for Extremal Black Holes in Higher Dimensions

All known stationary black hole solutions in higher dimensions possess additional rotational symmetries in addition to the stationary Killing field. Also, for all known stationary solutions, the event horizon is a Killing horizon, and the surface gravity is constant. In the case of non-degenerate horizons (non-extremal black holes), a general theorem was previously established [gr-qc/0605106] proving that these statements are in fact generally true under the assumption that the spacetime is analytic, and that the metric satisfies Einstein's equation. Here, we extend the analysis to the case of degenerate (extremal) black holes. It is shown that the theorem still holds true if the vector of angular velocities of the horizon satisfies a certain "diophantine condition," which holds except for a set of measure zero.Comment: 30pp, Latex, no figure

In-vivo optical monitoring of the efficacy of epidermal growth factor receptor targeted photodynamic therapy: The effect of fluence rate

Targeted photodynamic therapy (PDT) has the potential to improve the therapeutic effect of PDT due to significantly better tumor responses and less normal tissue damage. Here we investigated if the efficacy of epidermal growth factor receptor (EGFR) targeted PDT using cetuximab-IRDye700DX is fluence rate dependent. Cell survival after treatment with different fluence rates was investigated in three cell lines. Singlet oxygen formation was investigated using the singlet oxygen quencher sodium azide and singlet oxygen sensor green (SOSG). The long-term response (to 90 days) of solid OSC-19-luc2-cGFP tumors in mice was determined after illumination with 20, 50, or 150 mW·cm−2. Reflectance and fluorescence spectroscopy were used to monitor therapy. Singlet oxygen was formed during illumination as shown by the increase in SOSG fluorescence and the d

Topical photodynamic therapy using different porphyrin precursors leads to differences in vascular photosensitization and vascular damage in normal mouse skin

Different distributions of hexyl aminolevulinate (HAL), aminolevulinic acid (ALA) and methyl aminolevulinate (MAL) in the superficial vasculature are not well studied but they are hypothesized to play an important role in topical photodynamic therapy (PDT). The colocalization of fluorescent CD31 and protoporphyrin IX (PpIX) was calculated using confocal microscopy of mouse skin sections to investigate the vascular distribution after topical application. Vascular damage leads to disruption of the normal endothelial adherens junction complex, of which CD144 is an integral component. Therefore, normal CD31 combined with loss of normal fluorescent CD144 staining was visually scored to assess vascular damage. Both the vascular PpIX concentration and the vascular damage were highest for HAL, then ALA and then MAL. Vascular damage in MAL was not different from normal contralateral control skin. This pattern is consistent with literature data on vasoconstriction after PDT, and with the hypothesis that the vasculature plays a role in light fractionation that increases efficacy for HAL and ALA-PDT but not for MAL. These findings indicate that endothelial cells of superficial blood vessels synthesize biologically relevant PpIX concentrations, leading to vascular damage. Such vascular effects are expected to influence the oxygenation of tissue after PDT which can be important for treatment efficacy. The ability of the vasculature to synthesize PpIX and be damaged by PDT was compared between HAL, ALA and MAL in mouse skin using confocal microscopy and fluorescent CD31 and CD144 antibodies. Colocalization of CD31 and PpIX (left images) was calculated to measure endothelial PpIX synthesis. Vascular damage was scored as loss of normal CD144 staining (right images). Both PpIX synthesis and vascular damage were highest for HAL, then ALA, then MAL. This illustrates that superficial blood vessels synthesize biologically relevant amounts of PpIX. Vascular responses can limit oxygen supply during or after PDT and are expected to influence outcome

Lipid-mimicking phosphorus-based glycosidase inactivators as pharmacological chaperones for the treatment of Gaucher's disease

Gaucher's disease, the most prevalent lysosomal storage disorder, is caused by missense mutation of the GBA gene, ultimately resulting in deficient GCase activity, hence the excessive build-up of cellular glucosylceramide. Among different therapeutic strategies, pharmacological chaperoning of mutant GCase represents an attractive approach that relies on small organic molecules acting as protein stabilizers. Herein, we expand upon a new class of transient GCase inactivators based on a reactive 2-deoxy-2-fluoro-beta-d-glucoside tethered to an array of lipid-mimicking phosphorus-based aglycones, which not only improve the selectivity and inactivation efficiency, but also the stability of these compounds in aqueous media. This hypothesis was further validated with kinetic and cellular studies confirming restoration of catalytic activity in Gaucher cells after treatment with these pharmacological chaperones.Bio-organic Synthesi

The connection between computability of a nonlinear problem and its linearization: the Hartman-Grobman theorem revisited

As one of the seven open problems in the addendum to their 1989 book Computability in Analysis and Physics Pour-El and Richards (1989)[17], Pour-El and Richards asked, "What is the connection between the computability of the original nonlinear operator and the linear operator which results from it?" Yet at present, systematic studies of the issues raised by this question seem to be missing from the literature. In this paper, we study one problem in this direction: the Hartman-Grobman linearization theorem for ordinary differential equations (ODEs). We prove, roughly speaking, that near a hyperbolic equilibrium point x(0) of a nonlinear ODE (x) over dot = f(x), there is a computable homeomorphism H such that H circle phi = L circle H, where phi is the solution to the ODE and L is the solution to its linearization (x) over dot = Df (x(0)) x. (C) 2012 Elsevier B.V. All rights reserved.Fundacao para a Ciencia e a Tecnologia; EU FEDER POCTI/POCI via SQIG - Instituto de Telecomunicacoes through the FCT [PEst-OE/EEI/LA0008/2011

Safety and efficacy of intensive vs. guideline antiplatelet therapy in high-risk patients with recent ischemic stroke or transient ischemic attack: rationale and design of the Triple Antiplatelets for Reducing Dependency after Ischaemic Stroke (TARDIS) trial (ISRCTN47823388)

RATIONALE: The risk of recurrence following a stroke or transient ischemic attack is high, especially immediately after the event. HYPOTHESIS: Because two antiplatelet agents are superior to one in patients with non-cardioembolic events, more intensive treatment might be even more effective. SAMPLE SIZE ESTIMATES: The sample size of 4100 patients will allow a shift to less recurrence, and less severe recurrence, to be detected (odds ratio 0·68) with 90% power at 5% significance. METHODS AND DESIGN: Triple Antiplatelets for Reducing Dependency after Ischaemic Stroke (ISRCTN47823388) is comparing the safety and efficacy of intensive (combined aspirin, clopidogrel, and dipyridamole) vs. guideline antiplatelet therapy, both given for one-month. This international collaborative parallel-group prospective randomized open-label blinded-end-point phase III trial plans to recruit 4100 patients with acute ischemic stroke or transient ischemic attack. Randomization and data collection are performed over a secure Internet site with real-time data validation and concealment of allocation. Outcomes, serious adverse events, and neuroimaging are adjudicated centrally with blinding to treatment allocation. STUDY OUTCOME: The primary outcome is stroke recurrence and its severity ('ordinal recurrence' based on modified Rankin Scale) at 90 days, with masked assessment centrally by telephone. Secondary outcomes include vascular events, functional measures (disability, mood, cognition, quality of life), and safety (bleeding, death, serious adverse events). DISCUSSION: The trial has recruited more than 50% of its target sample size (latest number: 2399) and is running in 104 sites in 4 countries. One-third of patients presented with a transient ischemic attack