PRP and BMAC for Musculoskeletal Conditions via Biomaterial Carriers.

Platelet-rich plasma (PRP) and bone marrow aspirate concentrate (BMAC) are orthobiologic therapies considered as an alternative to the current therapies for muscle, bone and cartilage. Different formulations of biomaterials have been used as carriers for PRP and BMAC in order to increase regenerative processes. The most common biomaterials utilized in conjunction with PRP and BMAC clinical trials are organic scaffolds and natural or synthetic polymers. This review will cover the combinatorial strategies of biomaterial carriers with PRP and BMAC for musculoskeletal conditions (MsCs) repair and regeneration in clinical trials. The main objective is to review the therapeutic use of PRP and BMAC as a treatment option for muscle, bone and cartilage injuries

Hypoxia Augments Outgrowth Endothelial Cell (OEC) Sprouting and Directed Migration in Response to Sphingosine-1-Phosphate (S1P)

Therapeutic angiogenesis provides a promising approach to treat ischemic cardiovascular diseases through the delivery of proangiogenic cells and/or molecules. Outgrowth endothelial cells (OECs) are vascular progenitor cells that are especially suited for therapeutic strategies given their ease of noninvasive isolation from umbilical cord or adult peripheral blood and their potent ability to enhance tissue neovascularization. These cells are recruited to sites of vascular injury or tissue ischemia and directly incorporate within native vascular endothelium to participate in neovessel formation. A better understanding of how OEC activity may be boosted under hypoxia with external stimulation by proangiogenic molecules remains a challenge to improving their therapeutic potential. While vascular endothelial growth factor (VEGF) is widely established as a critical factor for initiating angiogenesis, sphingosine-1-phosphate (S1P), a bioactive lysophospholipid, has recently gained great enthusiasm as a potential mediator in neovascularization strategies. This study tests the hypothesis that hypoxia and the presence of VEGF impact the angiogenic response of OECs to S1P stimulation in vitro. We found that hypoxia altered the dynamically regulated S1P receptor 1 (S1PR1) expression on OECs in the presence of S1P (1.0 mu M) and/or VEGF (1.3 nM). the combined stimuli of S1P and VEGF together promoted OEC angiogenic activity as assessed by proliferation, wound healing, 3D sprouting, and directed migration under both normoxia and hypoxia. Hypoxia substantially augmented the response to S1P alone, resulting in similar to 6.5-fold and similar to 25-fold increases in sprouting and directed migration, respectively. Overall, this report highlights the importance of establishing hypoxic conditions in vitro when studying ischemia-related angiogenic strategies employing vascular progenitor cells.University of California, DavisAmerican Heart Association (AHA)Univ Calif Davis, Dept Biomed Engn, Davis, CA 95616 USAUniversidade Federal de São Paulo, Dept Biophys, São Paulo, BrazilUniv Calif Davis, Dept Neurobiol Physiol & Behav, Davis, CA 95616 USAUniversidade Federal de São Paulo, Dept Biophys, São Paulo, BrazilAmerican Heart Association (AHA): 15PRE22930044Web of Scienc

Demetallization of Enterococcus faecalis Biofilm: A preliminary study

Objectives: To determine the concentration of calcium, iron, manganese and zinc ions after the application of chelator to Enterococcus faecalis biofilms. Material and Methods: Fifty bovine maxillary central incisors were prepared and inoculated with E. faecalis for 60 days. The following were used as irrigation solutions: 17% EDTA (pH 3, 7 and 10), 2.5% sodium hypochlorite (NaOCl) combined with 17% EDTA (pH 3, 7 and 10), distilled water (pH 3, 7 and 10), and 2.5% NaOCl. Each solution was kept in the root canal for five minutes. Fifteen uncontaminated root canals were irrigated with 17% EDTA (pH 3, 7 and 10). Six teeth were used as bacterial control. The number of calcium, iron, manganese and zinc ions was determined using flame atomic absorption spectrometry. Mean ± standard deviation (SD) values were used for descriptive statistics. Results: Calcium chelation using 17% EDTA at pH 7 was higher than at pH 3 and 10, regardless of whether bacterial biofilm was present. The highest concentration of iron occurred at pH 3 in the presence of bacterial biofilm. The highest concentration of manganese found was 2.5% NaOCl and 17% EDTA at pH 7 in the presence of bacterial biofilm. Zinc levels were not detectable. Conclusions: The pH of chelating agents affected the removal of calcium, iron, and manganese ions. The concentration of iron ions in root canals with bacterial biofilm was higher after the use of 17% EDTA at pH 3 than after the use of the other solutions at all pH levels

Demetallization of Enterococcus faecalis biofilm: a preliminary study

Objectives To determine the concentration of calcium, iron, manganese and zinc ions after the application of chelator to Enterococcus faecalis biofilms. Material and Methods Fifty bovine maxillary central incisors were prepared and inoculated with E. faecalis for 60 days. The following were used as irrigation solutions: 17% EDTA (pH 3, 7 and 10), 2.5% sodium hypochlorite (NaOCl) combined with 17% EDTA (pH 3, 7 and 10), distilled water (pH 3, 7 and 10), and 2.5% NaOCl. Each solution was kept in the root canal for five minutes. Fifteen uncontaminated root canals were irrigated with 17% EDTA (pH 3, 7 and 10). Six teeth were used as bacterial control. The number of calcium, iron, manganese and zinc ions was determined using flame atomic absorption spectrometry. Mean ± standard deviation (SD) values were used for descriptive statistics. Results Calcium chelation using 17% EDTA at pH 7 was higher than at pH 3 and 10, regardless of whether bacterial biofilm was present. The highest concentration of iron occurred at pH 3 in the presence of bacterial biofilm. The highest concentration of manganese found was 2.5% NaOCl and 17% EDTA at pH 7 in the presence of bacterial biofilm. Zinc levels were not detectable. Conclusions The pH of chelating agents affected the removal of calcium, iron, and manganese ions. The concentration of iron ions in root canals with bacterial biofilm was higher after the use of 17% EDTA at pH 3 than after the use of the other solutions at all pH levels

Fipronil effect on the frequency of anomalous brood in honeybee reared in vitro

Larvae of honeybee workers were exposed to the insecticide fipronil during the feeding phase. To evaluate the effect of fipronil in the post-embryonic development of africanized Apis mellifera, bioassays of toxicity were done. The bioassays were performed by acute exposure applying 1μL of distilled water for control (I) and for experiments: 0.5 ng a.i./μL of fipronil; 5 ng a.i./μL of fipronil and 20 ng a.i./ μL of fipronil. Triplicates were performed for all treatments. The results showed that the rate of anomalous pupae in exposed honeybees was statistically significant in relationship to the control (p <0:03). The most frequent abnormalities were: high pigmentation on the proximal and distal larval body and body malformation, such as absence of head and limbs. Pink eye pupa and white eyed pupae presented malformations in their larval bodies, but with the eye developed. It is assumed that the fat body is related to the high rate of anomalies, since this tissue has proteins linked to the process of metamorphosis. Furthermore, the fat body may be participating in the regulation of juvenile hormone during the process of metamorphosis, and consequently in the release of ecdysteroid hormones that are involved in the change from larva to adult. The high rate of abnormalities in the pupal stage of individuals exposed to fipronil raises concerns about the impacts caused in the colonies of bees and population decline of pollinators. Keywords: bees, larvae, pupae, metamorphosis, anomalies, fiproni

The Implementation of Teleconsultations in a Physiotherapy Service During COVID-19 Pandemic in Brazil: A Case Report

Introduction: The Brazilian Council of Physiotherapy and Occupational Therapy regulated the use of teleconsultation during the COVID-19 pandemic, creating uncertainty about its use in Brazil. Objective: To describe the experience of teleconsultations during the COVID-19 pandemic. Methods: Four patients participated in the study with the following diagnoses: Parkinson’s disease, stroke, peripheral facial paralysis, and tibial plateau fracture. Patients underwent up to 10 physiotherapy sessions via digital tools.  The 5-A self-management tool (Assess, Advise, Agree, Assist, Arrange) guided the sessions. Results: The teleconsultation type varied between synchronous (n = 1; 25%); asynchronous (n = 2; 50%) and synchronous/asynchronous (n = 1; 25%). There was 75% (n = 3) adherence and one withdrawal (25%). As the benefits of teleconsultations, the patients pointed out the convenience, maintenance of the exercises, and contact with the professional. The reported limitations were the lack of the use of physiotherapeutic devices. Conclusion: Teleconsultations contribute to the continuity of physiotherapy treatment during social isolation. Adherence to treatment was facilitated by access to the technology and by offering patients the choice of teleconsultation type. &nbsp

TERRA DOS MORTOS, LUGAR DOS VIVOS: O CEMITÉRIO SANTANA COMO PATRIMÔNIO CULTURAL DA CIDADE DE GOIÂNIA (GO)

A partir da ampliação do conceito de patrimônio ocorrido a parir da segunda metade do século XX, o presente artigo pretende investigar quando e como os cemitérios foram incluídos no rol de bens culturais passiveis de patrimonialização. Para tanto, recorremos à História da cultura Material como norteador teórico-metodológico e às Cartas Patrimoniais como suporte documental para a presente análise. O Cemitério Santana na cidade de Goiânia (GO) é o lócus escolhido para abordarmos aspectos inerentes às perspectivas que foram salutares para a entrada dos cemitérios na lista do patrimônio histórico cultural – sobretudo a memória e a estética. Do ponto de vista da análise estética, buscamos nos postulados dos filósofos Longino, Edmund Burke e Immanuel Kant a ideia de sublime aplicada na interpretação da arte tumular do referido cemitério

PRODUÇÃO DE MARGARINA EXPERIMENTAL POR MEIO DA INTERESTERIFICAÇÃO QUÍMICA E ENZIMÁTICA COM UMA MISTURA DE ESTEARINA DE PALMA, ÓLEO DE COCO E ÓLEO DE CANOLA

A alta ingestão de gordura trans está associada a várias doenças crônicas, como doenças cardiovasculares e câncer . A interesterificação de gorduras pode melhorar certas propriedades físico-químicas e nutracêuticas por modificação do seu perfil de triacilglicerol e conteúdo de gordura sólida. Usando como catalisadores, lipase específica sn - 1,3, os ácidos graxos originais na posição sn - 2, são mantidas com os benefícios nutricionais. A incorporação de óleo de canola permite aumentar a quantidade de ácido oleico, com benefícios nutricionais para o produto final. O objetivo, neste estudo, foi a produção de lipídios estruturados com estearina de palma, óleo de coco e óleo de oliva, a serem incorporados em margarinas, por interesterificação enzimática catalisada por lipases imobilizadas e química com metóxido de sódio. As gorduras foram misturadas em uma proporção de 45% de estearina de palma, 30% de óleo de coco e 25% de óleo de canola e submetidas à interesterificação enzimática utilizando contínua sn-1,3 lipase específica (Lipozyme® RM IM) a 65 °C e 32 horas. Após a reação, as propriedades físicas (perfil de ácidos graxos e consistência) dos produtos interesterificados e a mistura física foram determinadas. Gorduras trans-livres interesterificadas enzimaticamente e quimicamente foram usadas para produzir margarinas em uma planta piloto. As margarinas produzidas a partir de gorduras interesterificadas químicas e enzimáticas mostraram a melhor consistência do que a margarina obtida a partir da mistura de gorduras originais. Portanto, lipídios estruturados à base de estearina de palma, óleo de coco e óleo de canola foram adequados para a formulação de margarinas trans-livres com propriedades de textura desejáveis.Palavras-chave: Consistência. Lipase. Lipozime RM. Interesterificação enzimática contínua. Lipídios estruturados.Financiadores: FAPESP, CNPq, CAPES