2-(Adamantan-1-yl)-1,3-bis(4-methylphenyl)propan-2-ol

The conformation of the title compound, C27H34O, is stabilized by a weak intramolecular C - H⋯π interaction. The dihedral angle between the benzene rings is 54.79(4)°. The adamantane cage consists of three fused cyclohexane rings in classical chair conformations, with C - C - C angles in the range 107.75(10)-111.35(9)°. Although the molecule contains a hydroxy group as a conceivable hydrogen-bond donor, this group is sterically hindered by bulky substituents and no hydrogen bonds are observed in the crystal structure.Internal Founding Agency of Tomas Bata University in Zlin [IGA/FT/2012/016

N-benzyl-9-isopropyl-9H-purin-6-amine

The asymmetric unit of the title compound, C15H17N5, consists of two molecules in which the dihedral angles between the best planes of the purine ring system (r.m.s. deviations = 0.0060 and 0.0190 Å) and the benzene ring are 89.21 (3) and 82.14 (4)°. The molecules within the asymmetric unit are linked into dimers by pairs of N - H⋯N hydrogen bonds. Weak C - H⋯π contacts and π-π interactions [centroid-centroid = 3.3071 (1) Å] further connect the molecules into a three-dimensional network. © Gergela et al. 2013.Internal Founding Agency of Tomas Bata University in Zlin [IGA/FT/2013/008

A first survey of the rye (Secale cereale) genome composition through BAC end sequencing of the short arm of chromosome 1R

<p>Abstract</p> <p>Background</p> <p>Rye (<it>Secale cereale </it>L.) belongs to tribe Triticeae and is an important temperate cereal. It is one of the parents of man-made species Triticale and has been used as a source of agronomically important genes for wheat improvement. The short arm of rye chromosome 1 (1RS), in particular is rich in useful genes, and as it may increase yield, protein content and resistance to biotic and abiotic stress, it has been introgressed into wheat as the 1BL.1RS translocation. A better knowledge of the rye genome could facilitate rye improvement and increase the efficiency of utilizing rye genes in wheat breeding.</p> <p>Results</p> <p>Here, we report on BAC end sequencing of 1,536 clones from two 1RS-specific BAC libraries. We obtained 2,778 (90.4%) useful sequences with a cumulative length of 2,032,538 bp and an average read length of 732 bp. These sequences represent 0.5% of 1RS arm. The GC content of the sequenced fraction of 1RS is 45.9%, and at least 84% of the 1RS arm consists of repetitive DNA. We identified transposable element junctions in BESs and developed insertion site based polymorphism markers (ISBP). Out of the 64 primer pairs tested, 17 (26.6%) were specific for 1RS. We also identified BESs carrying microsatellites suitable for development of 1RS-specific SSR markers.</p> <p>Conclusion</p> <p>This work demonstrates the utility of chromosome arm-specific BAC libraries for targeted analysis of large Triticeae genomes and provides new sequence data from the rye genome and molecular markers for the short arm of rye chromosome 1.</p

A many-analysts approach to the relation between religiosity and well-being

The relation between religiosity and well-being is one of the most researched topics in the psychology of religion, yet the directionality and robustness of the effect remains debated. Here, we adopted a many-analysts approach to assess the robustness of this relation based on a new cross-cultural dataset (N=10,535 participants from 24 countries). We recruited 120 analysis teams to investigate (1) whether religious people self-report higher well-being, and (2) whether the relation between religiosity and self-reported well-being depends on perceived cultural norms of religion (i.e., whether it is considered normal and desirable to be religious in a given country). In a two-stage procedure, the teams first created an analysis plan and then executed their planned analysis on the data. For the first research question, all but 3 teams reported positive effect sizes with credible/confidence intervals excluding zero (median reported β=0.120). For the second research question, this was the case for 65% of the teams (median reported β=0.039). While most teams applied (multilevel) linear regression models, there was considerable variability in the choice of items used to construct the independent variables, the dependent variable, and the included covariates

A Many-analysts Approach to the Relation Between Religiosity and Well-being

The relation between religiosity and well-being is one of the most researched topics in the psychology of religion, yet the directionality and robustness of the effect remains debated. Here, we adopted a many-analysts approach to assess the robustness of this relation based on a new cross-cultural dataset (N = 10, 535 participants from 24 countries). We recruited 120 analysis teams to investigate (1) whether religious people self-report higher well-being, and (2) whether the relation between religiosity and self-reported well-being depends on perceived cultural norms of religion (i.e., whether it is considered normal and desirable to be religious in a given country). In a two-stage procedure, the teams first created an analysis plan and then executed their planned analysis on the data. For the first research question, all but 3 teams reported positive effect sizes with credible/confidence intervals excluding zero (median reported β = 0.120). For the second research question, this was the case for 65% of the teams (median reported β = 0.039). While most teams applied (multilevel) linear regression models, there was considerable variability in the choice of items used to construct the independent variables, the dependent variable, and the included covariates

Insights into the accuracy of social scientists' forecasts of societal change

How well can social scientists predict societal change, and what processes underlie their predictions? To answer these questions, we ran two forecasting tournaments testing the accuracy of predictions of societal change in domains commonly studied in the social sciences: ideological preferences, political polarization, life satisfaction, sentiment on social media, and gender–career and racial bias. After we provided them with historical trend data on the relevant domain, social scientists submitted pre-registered monthly forecasts for a year (Tournament 1; N = 86 teams and 359 forecasts), with an opportunity to update forecasts on the basis of new data six months later (Tournament 2; N = 120 teams and 546 forecasts). Benchmarking forecasting accuracy revealed that social scientists’ forecasts were on average no more accurate than those of simple statistical models (historical means, random walks or linear regressions) or the aggregate forecasts of a sample from the general public (N = 802). However, scientists were more accurate if they had scientific expertise in a prediction domain, were interdisciplinary, used simpler models and based predictions on prior data

Shifting the limits in wheat research and breeding using a fully annotated reference genome

Introduction: Wheat (Triticum aestivum L.) is the most widely cultivated crop on Earth, contributing about a fifth of the total calories consumed by humans. Consequently, wheat yields and production affect the global economy, and failed harvests can lead to social unrest. Breeders continuously strive to develop improved varieties by fine-tuning genetically complex yield and end-use quality parameters while maintaining stable yields and adapting the crop to regionally specific biotic and abiotic stresses. Rationale: Breeding efforts are limited by insufficient knowledge and understanding of wheat biology and the molecular basis of central agronomic traits. To meet the demands of human population growth, there is an urgent need for wheat research and breeding to accelerate genetic gain as well as to increase and protect wheat yield and quality traits. In other plant and animal species, access to a fully annotated and ordered genome sequence, including regulatory sequences and genome-diversity information, has promoted the development of systematic and more time-efficient approaches for the selection and understanding of important traits. Wheat has lagged behind, primarily owing to the challenges of assembling a genome that is more than five times as large as the human genome, polyploid, and complex, containing more than 85% repetitive DNA. To provide a foundation for improvement through molecular breeding, in 2005, the International Wheat Genome Sequencing Consortium set out to deliver a high-quality annotated reference genome sequence of bread wheat. Results: An annotated reference sequence representing the hexaploid bread wheat genome in the form of 21 chromosome-like sequence assemblies has now been delivered, giving access to 107,891 high-confidence genes, including their genomic context of regulatory sequences. This assembly enabled the discovery of tissue- and developmental stage–related gene coexpression networks using a transcriptome atlas representing all stages of wheat development. The dynamics of change in complex gene families involved in environmental adaptation and end-use quality were revealed at subgenome resolution and contextualized to known agronomic single-gene or quantitative trait loci. Aspects of the future value of the annotated assembly for molecular breeding and research were exemplarily illustrated by resolving the genetic basis of a quantitative trait locus conferring resistance to abiotic stress and insect damage as well as by serving as the basis for genome editing of the flowering-time trait. Conclusion: This annotated reference sequence of wheat is a resource that can now drive disruptive innovation in wheat improvement, as this community resource establishes the foundation for accelerating wheat research and application through improved understanding of wheat biology and genomics-assisted breeding. Importantly, the bioinformatics capacity developed for model-organism genomes will facilitate a better understanding of the wheat genome as a result of the high-quality chromosome-based genome assembly. By necessity, breeders work with the genome at the whole chromosome level, as each new cross involves the modification of genome-wide gene networks that control the expression of complex traits such as yield. With the annotated and ordered reference genome sequence in place, researchers and breeders can now easily access sequence-level information to precisely define the necessary changes in the genomes for breeding programs. This will be realized through the implementation of new DNA marker platforms and targeted breeding technologies, including genome editing

Validity of primary motor area localization with fMRI versus electric cortical stimulation: A comparative study.

During my postgraduate studies, I have been working on the problems of surgery on eloquently located intrinsic brain tumors in a wider context. Towards the end of my research work, the technically challenging insular glioma surgery became the main topic. ln general, the radicality of the resection is an acceptable favourable prognostic factor even in low grade gliomas. However, for a safe resection to be possible, a mere knowledge of anatomy is not sufficient. In cases, where a suspicion of an eloquent tumor location arises, careful consideration should be made regarding the the position and the relationship of the functional cortex to the tumor. This consideration can be aided by the use of functional magnetic resonance results. ln our study, functional magnetic resonance was reliable in 83% of patients operated on a tumor in the central motor area (15/18). The distances between the fMRI activated cluster and the ECS targets were within the range of 5mm (error inherent for target-Iocalizing accuracy of neuronavigation system) in 20/26 of our measurements. The spread of the BOLD activation volume is significantly lower during more restrictive corrected thresholding P<0.05c. In all patients with this fMRI threshold, the concordance with the ECS result was absolute. If the volume of the gained corrected fMRI..

Validity of primary motor area localization with fMRI versus electric cortical stimulation: A comparative study.

During my postgraduate studies, I have been working on the problems of surgery on eloquently located intrinsic brain tumors in a wider context. Towards the end of my research work, the technically challenging insular glioma surgery became the main topic. ln general, the radicality of the resection is an acceptable favourable prognostic factor even in low grade gliomas. However, for a safe resection to be possible, a mere knowledge of anatomy is not sufficient. In cases, where a suspicion of an eloquent tumor location arises, careful consideration should be made regarding the the position and the relationship of the functional cortex to the tumor. This consideration can be aided by the use of functional magnetic resonance results. ln our study, functional magnetic resonance was reliable in 83% of patients operated on a tumor in the central motor area (15/18). The distances between the fMRI activated cluster and the ECS targets were within the range of 5mm (error inherent for target-Iocalizing accuracy of neuronavigation system) in 20/26 of our measurements. The spread of the BOLD activation volume is significantly lower during more restrictive corrected thresholding P<0.05c. In all patients with this fMRI threshold, the concordance with the ECS result was absolute. If the volume of the gained corrected fMRI..

Validity of primary motor area localization with fMRI versus electric cortical stimulation: A comparative study.

Během mého postgraduálního studia jsem se zaměřil na problematiku operativy elokventně uložených intrinsických nádorů mozku v širším kontextu, v závěru práce jsem se věnoval operativě gliomů insuly, která je technicky náročná. Obecně, radikalita resekce je zřejmě i v případě nízkostupňového gliomu akceptovatelným prognosticky příznivým faktorem. Pro bezpečnou resekci však samotná znalost anatomie není postačující, v případech suspekce na eloquentní lokalizaci tumoru musí následovat rozvaha o poloze funkční oblasti vzhledem k tumoru. Tuto rozvahu může posunout vpřed výsledek funkční magnetické rezonance. Funkční magnetická rezonance byla v naší studii spolehlivá u 83% pacientů operovaných pro nádor v centrální motorické oblasti (15/18). Vzdálenosti mezi fMRI aktivovanými clustery a cílem ECS se nacházely v rozmezí 5mm (hranice spolehlivosti neuronavigačního přístroje) u 20 z 26 našich měření. Menší rozptyl objemu BOLD aktivace je patrný při více restriktivním korigovaném prahování P&lt;0.05c. U všech takto konzervativně prahovaných vyšetření panovala shoda s výsledkem ECS. Pokud bylo dosažené korigované vyšetření objemově dostatečné pro lokalizaci předpokládané SMl, pravděpodobnost validity byla vyšší než v případě, kdy jsme museli použít nekorigované vyšetření. Senzitivita fMRI pro lokalizaci mozkové kůry s...During my postgraduate studies, I have been working on the problems of surgery on eloquently located intrinsic brain tumors in a wider context. Towards the end of my research work, the technically challenging insular glioma surgery became the main topic. ln general, the radicality of the resection is an acceptable favourable prognostic factor even in low grade gliomas. However, for a safe resection to be possible, a mere knowledge of anatomy is not sufficient. In cases, where a suspicion of an eloquent tumor location arises, careful consideration should be made regarding the the position and the relationship of the functional cortex to the tumor. This consideration can be aided by the use of functional magnetic resonance results. ln our study, functional magnetic resonance was reliable in 83% of patients operated on a tumor in the central motor area (15/18). The distances between the fMRI activated cluster and the ECS targets were within the range of 5mm (error inherent for target-Iocalizing accuracy of neuronavigation system) in 20/26 of our measurements. The spread of the BOLD activation volume is significantly lower during more restrictive corrected thresholding P&lt;0.05c. In all patients with this fMRI threshold, the concordance with the ECS result was absolute. If the volume of the gained corrected fMRI...Institute of Anatomy First Faculty of Medicine Charles University in PragueAnatomický ústav 1. LF UK v PrazeFirst Faculty of Medicine1. lékařská fakult