Grandes branquiópodos (Crustacea: Branchiopoda: Anostraca, Notostraca) en la provincia de Málaga (España) (año hidrológico 2012/2013)

Grans branquiòpodes (Crustacea, Branchiopoda: Anostraca, Notostraca) a la província de Màlaga, Espanya (any hidrològic 2012/2013) S'enumeren les cites d'una campanya de mostratge de grans branquiòpodes portada a terme a la província de Màlaga (Andalusia, sud d'Espanya) que ha permès la detecció de cinc espècies (Branchipus cortesi, Chirocephalus diaphanus, Streptocephalus torvicornis, Triops mauritanicus aggr. i Phallocryptus spinosa) en 90 masses d'aigua mostrejades.Large branchiopods (Crustacea, Branchiopoda, Anostraca, Notostraca) from Málaga province, Spain (2012/2013 hydrological year) This paper presents the occurrence of the large branchiopods detected during a survey carried out in the province of Málaga (Andalusia, southern Spain). Five species (Branchipus cortesi, Chirocephalus diaphanus, Streptocephalus torvicornis, Triops mauritanicus aggr. and Phallocryptus spinosa) were recorded at 90 sampled wetlands.Se enumeran las citas de una campaña de muestreo de grandes branquiópodos realizada en la provincia de Málaga (Andalucía, sur de España) que ha permitido la detección de cinco especies (Branchipus cortesi, Chirocephalus diaphanus, Streptocephalus torvicornis, Triops mauritanicus aggr. y Phallocryptus spinosa) en 90 masas de agua muestreadas

Antecedentes y consecuentes psicológicos del cambio de empleo en los jóvenes

Las investigaciones recientes centradas en el estudio de los antecedentes y consecuentes del cambio de empleo sugieren que este cambio está influido por una evaluación negativa del anterior empleo y puede tener consecuencias positivas para el individuo. Estos resultados proceden de estudios realizados con muestras de profesionales y directivos. Sin embargo, puede que estos resultados no sean generalizables a muestras de trabajadores con un nivel de cualificación menor. El objetivo del presente estudio es analizar los antecedentes y consecuentes psicológicos del cambio de empleo en una muestra de jóvenes pertenecientes a dos grupos ocupacionales: opereradores y aprendices de sistemas de información complejos y operarios del sector del metal. Los resultados indican que la satisfacción laboral está influida por el cambio de empleo. Sin embargo, el bienestar psicológico, el conflicto y la ambigüedad de rol y la centralidad del trabajo son variables que no están relacionadas con el cambio de empleo. Estos resultados son interpretados en función de la literatura revisada sobre el cambio de empleo.Recent research investigating antecedents and consequences of job change suggests that change is influenced by a negative evaluation of the previous job and it has positive outcomes for the individual. Tliose results proceed from studies with professional and white-collar samples. It may be that the conclusions of these studies would be inappropiate for blue-collar workers. This paper aims to analyse the psychological antecedents and consequences of job change amongst young workers who belong to two occupational groups: operators of office technology and machine operators in production manufacturing in the metal industry. It was found that job satisfaction is influenced by the job change while psychological well-being, role conflict and ambiguity, and work centrality are not related to the job change. These results are discussed in relation to the recent research on job change

The drivers of SME innovation in the regions of the EU

European Union (EU) innovation policies have for long remained mostly research driven. The fundamental goal has been to achieve a rate of R&D investment of 3% of GDP. Small and medium-sized enterprise (SME) innovation, however, relies on a variety of internal sources —both R&D and non-R&D based— and external drivers, such as collaboration with other firms and research centres, and is profoundly influence by location and context. Given this multiplicity of innovation activities, this study argues that innovation policies fundamentally based on a place-blind increase of R&D investment may not deliver the best outcomes in regions where the capacity of SMEs is to benefit from R&D is limited. We posit that collaboration and regional specificities can play a greater role in determining SME innovation, beyond just R&D activities. Using data from the Regional Innovation Scoreboard (RIS), covering 220 regions across 22 European countries, we find that regions in Europe differ significantly in terms of SME innovation depending on their location. SMEs in more innovative regions benefit to a far greater extent from a combination of internal R&D, external collaboration of all sorts, and non-R&D inputs. SMEs in less innovative regions rely fundamentally on external sources and, particularly, on collaboration with other firms. Greater investment in public R&D does not always lead to improvements in regional SME innovation, regardless of context. Collaboration is a central innovation activity that can complement R&D, showing an even stronger effect on SME innovation than R&D. Hence, a more collaboration-based and place-sensitive policy is required to maximise SME innovation across the variety of European regional contexts

Identification of a Novel HIF-1α-α_{M}β_{2} Integrin-NET Axis in Fibrotic Interstitial Lung Disease

Neutrophilic inflammation correlates with mortality in fibrotic interstitial lung disease (ILD) particularly in the most severe form, idiopathic pulmonary fibrosis (IPF), although the underlying mechanisms remain unclear. Neutrophil function is modulated by numerous factors, including integrin activation, inflammatory cytokines and hypoxia. Hypoxia has an important role in inflammation and may also contribute to pulmonary disease. We aimed to determine how neutrophil activation occurs in ILD and the relative importance of hypoxia. Using lung biopsies and bronchoalveolar lavage (BAL) fluid from ILD patients we investigated the extent of hypoxia and neutrophil activation in ILD lungs. Then we used ex vivo neutrophils isolated from healthy volunteers and BAL from patients with ILD and non-ILD controls to further investigate aberrant neutrophil activation in hypoxia and ILD. We demonstrate for the first time using intracellular staining, HIF-1α stabilization in neutrophils and endothelial cells in ILD lung biopsies. Hypoxia enhanced both spontaneous (+1.31-fold, p < 0.05) and phorbol 12-myristate 13-acetate (PMA)-induced (+1.65-fold, p < 0.001) neutrophil extracellular trap (NET) release, neutrophil adhesion (+8.8-fold, <0.05), and trans-endothelial migration (+1.9-fold, p < 0.05). Hypoxia also increased neutrophil expression of the αM (+3.1-fold, p < 0.001) and αX (+1.6-fold, p < 0.01) integrin subunits. Interestingly, NET formation was induced by αMβ2 integrin activation and prevented by cation chelation. Finally, we observed NET-like structures in IPF lung sections and in the BAL from ILD patients, and quantification showed increased cell-free DNA content (+5.5-fold, p < 0.01) and MPO-citrullinated histone H3 complexes (+21.9-fold, p < 0.01) in BAL from ILD patients compared to non-ILD controls. In conclusion, HIF-1α upregulation may augment neutrophil recruitment and activation within the lung interstitium through activation of β2 integrins. Our results identify a novel HIF-1α- αMβ2 integrin axis in NET formation for future exploration in therapeutic approaches to fibrotic ILD

Ground states and dynamics of population-imbalanced Fermi condensates in one dimension

By using the numerically exact density-matrix renormalization group (DMRG) approach, we investigate the ground states of harmonically trapped one-dimensional (1D) fermions with population imbalance and find that the Larkin-Ovchinnikov (LO) state, which is a condensed state of fermion pairs with nonzero center-of-mass momentum, is realized for a wide range of parameters. The phase diagram comprising the two phases of i) an LO state at the trap center and a balanced condensate at the periphery and ii) an LO state at the trap center and a pure majority component at the periphery, is obtained. The reduced two-body density matrix indicates that most of the minority atoms contribute to the LO-type quasi-condensate. With the time-dependent DMRG, we also investigate the real-time dynamics of a system of 1D fermions in response to a spin-flip excitation.Comment: 20 pages, 15 figures, accepted for publication in New Journal of Physic

Identification of a novel HIF-1α-αMβ2 integrin-NETosis axis in fibrotic interstitial lung disease

Neutrophilic inflammation correlates with mortality in fibrotic interstitial lung disease (ILD) particularly in the most severe form, idiopathic pulmonary fibrosis (IPF), although the underlying mechanisms remain unclear. Neutrophil function is modulated by numerous factors, including integrin activation, inflammatory cytokines and hypoxia. Hypoxia has an important role in inflammation and may also contribute to pulmonary disease. We aimed to determine how neutrophil activation occurs in ILD and the relative importance of hypoxia. Using lung biopsies and bronchoalveolar lavage (BAL) fluid from ILD patients we investigated the extent of hypoxia and neutrophil activation in ILD lungs. Then we used ex vivo neutrophils isolated from healthy volunteers and BAL from patients with ILD and non-ILD controls to further investigate aberrant neutrophil activation in hypoxia and ILD. We demonstrate for the first time using intracellular staining, HIF-1α stabilization in neutrophils and endothelial cells in ILD lung biopsies. Hypoxia enhanced both spontaneous (+1.31-fold, p < 0.05) and phorbol 12-myristate 13-acetate (PMA)-induced (+1.65-fold, p < 0.001) neutrophil extracellular trap (NET) release, neutrophil adhesion (+8.8-fold, <0.05), and trans-endothelial migration (+1.9-fold, p < 0.05). Hypoxia also increased neutrophil expression of the αM (+3.1-fold, p < 0.001) and αX (+1.6-fold, p < 0.01) integrin subunits. Interestingly, NET formation was induced by αMβ2 integrin activation and prevented by cation chelation. Finally, we observed NET-like structures in IPF lung sections and in the BAL from ILD patients, and quantification showed increased cell-free DNA content (+5.5-fold, p < 0.01) and MPO-citrullinated histone H3 complexes (+21.9-fold, p < 0.01) in BAL from ILD patients compared to non-ILD controls. In conclusion, HIF-1α upregulation may augment neutrophil recruitment and activation within the lung interstitium through activation of β2 integrins. Our results identify a novel HIF-1α- αMβ2 integrin axis in NET formation for future exploration in therapeutic approaches to fibrotic ILD

Spatiotemporal Dynamics of Genetic Variation in the Iberian Lynx along Its Path to Extinction Reconstructed with Ancient DNA

There is the tendency to assume that endangered species have been both genetically and demographically healthier in the past, so that any genetic erosion observed today was caused by their recent decline. The Iberian lynx (Lynx pardinus) suffered a dramatic and continuous decline during the 20th century, and now shows extremely low genome- and specieswide genetic diversity among other signs of genomic erosion. We analyze ancient (N¼10), historical (N¼245), and contemporary (N¼172) samples with microsatellite and mitogenome data to reconstruct the species’ demography and investigate patterns of genetic variation across space and time. Iberian lynx populations transitioned from low but significantly higher genetic diversity than today and shallow geographical differentiation millennia ago, through a structured metapopulation with varying levels of diversity during the last centuries, to two extremely genetically depauperate and differentiated remnant populations by 2002. The historical subpopulations show varying extents of genetic drift in relation to their recent size and time in isolation, but these do not predict whether the populations persisted or went finally extinct. In conclusion, current genetic patterns were mainly shaped by genetic drift, supporting the current admixture of the two genetic pools and calling for a comprehensive genetic management of the ongoing conservation program. This study illustrates how a retrospective analysis of demographic and genetic patterns of endangered species can shed light onto their evolutionary history and this, in turn, can inform conservation actions

Clinical Risk Score to Predict Pathogenic Genotypes in Patients With Dilated Cardiomyopathy

Background: Although genotyping allows family screening and influences risk-stratification in patients with nonischemic dilated cardiomyopathy (DCM) or isolated left ventricular systolic dysfunction (LVSD), its result is negative in a significant number of patients, limiting its widespread adoption. Objectives: This study sought to develop and externally validate a score that predicts the probability for a positive genetic test result (G+) in DCM/LVSD. Methods: Clinical, electrocardiogram, and echocardiographic variables were collected in 1,015 genotyped patients from Spain with DCM/LVSD. Multivariable logistic regression analysis was used to identify variables independently predicting G+, which were summed to create the Madrid Genotype Score. The external validation sample comprised 1,097 genotyped patients from the Maastricht and Trieste registries. Results: A G+ result was found in 377 (37%) and 289 (26%) patients from the derivation and validation cohorts, respectively. Independent predictors of a G+ result in the derivation cohort were: family history of DCM (OR: 2.29; 95% CI: 1.73-3.04; P &lt; 0.001), low electrocardiogram voltage in peripheral leads (OR: 3.61; 95% CI: 2.38-5.49; P &lt; 0.001), skeletal myopathy (OR: 3.42; 95% CI: 1.60-7.31; P = 0.001), absence of hypertension (OR: 2.28; 95% CI: 1.67-3.13; P &lt; 0.001), and absence of left bundle branch block (OR: 3.58; 95% CI: 2.57-5.01; P &lt; 0.001). A score containing these factors predicted a G+ result, ranging from 3% when all predictors were absent to 79% when ≥4 predictors were present. Internal validation provided a C-statistic of 0.74 (95% CI: 0.71-0.77) and a calibration slope of 0.94 (95% CI: 0.80-1.10). The C-statistic in the external validation cohort was 0.74 (95% CI: 0.71-0.78). Conclusions: The Madrid Genotype Score is an accurate tool to predict a G+ result in DCM/LVSD