Tile-Based Two-Dimensional Phase Unwrapping for Digital Holography Using a Modular Framework

A variety of physical and biomedical imaging techniques, such as digital holography, interferometric synthetic aperture radar (InSAR), or magnetic resonance imaging (MRI) enable measurement of the phase of a physical quantity additionally to its amplitude. However, the phase can commonly only be measured modulo 2π, as a so called wrapped phase map. Phase unwrapping is the process of obtaining the underlying physical phase map from the wrapped phase. Tile-based phase unwrapping algorithms operate by first tessellating the phase map, then unwrapping individual tiles, and finally merging them to a continuous phase map. They can be implemented computationally efficiently and are robust to noise. However, they are prone to failure in the presence of phase residues or erroneous unwraps of single tiles. We tried to overcome these shortcomings by creating novel tile unwrapping and merging algorithms as well as creating a framework that allows to combine them in modular fashion. To increase the robustness of the tile unwrapping step, we implemented a model-based algorithm that makes efficient use of linear algebra to unwrap individual tiles. Furthermore, we adapted an established pixel-based unwrapping algorithm to create a quality guided tile merger. These original algorithms as well as previously existing ones were implemented in a modular phase unwrapping C++ framework. By examining different combinations of unwrapping and merging algorithms we compared our method to existing approaches. We could show that the appropriate choice of unwrapping and merging algorithms can significantly improve the unwrapped result in the presence of phase residues and noise. Beyond that, our modular framework allows for efficient design and test of new tile-based phase unwrapping algorithms. The software developed in this study is freely available

Boundary Conditions of the Heliosphere

Radiative transfer equilibrium models of nearby interstellar matter (ISM) yield the boundary conditions of the heliosphere when constrained with observations of ISM inside and outside of the heliosphere. Filtration factors for interstellar neutrals crossing the heliosheath region, from charge exchange with interstellar plasma, are given for H, He, N, O, Ar, and Ne. The best models predict n(HI)~0.2 /cc, n(e)~0.1 /cc, however if the isotropic 2 kHz emission observed by Voyager (Kurth & Gurnett 2003) is formed in the surrounding ISM, an alternate model with lower electron densities is indicated. Observations of nearby ISM, the radiative transfer models, and historical 10Be records provide information on past variations in the galactic environment of the Sun

Three-dimensional imaging of intracochlear tissue by scanning laser optical tomography (SLOT)

The presented study focuses on the application of scanning laser optical tomography (SLOT) for non-destructive visualization of anatomical structures inside the human cochlea ex vivo. SLOT is a laser-based highly efficient microscopy technique, which allows for tomographic imaging of the internal structure of transparent large-scale specimens (up to 1 cm3). Thus, in the field of otology this technique is best convenient for an ex vivo study of the inner ear anatomy. For this purpose, the preparation before imaging comprises mechanically assisted decalcification, dehydration as well as optical clearing of the cochlea samples. Here, we demonstrate results of SLOT visualizing hard and soft tissue structures of the human cochlea with an optical resolution in the micrometer range using absorption and autofluorescence as contrast mechanisms. Furthermore, we compare our results with the method of X-ray micro tomography (micro-CT, μCT) as clinical gold standard which is based only on absorption. In general, SLOT can provide the advantage of covering all contrast mechanisms known from other light microscopy techniques, such as fluorescence or scattering. For this reason, a protocol for antibody staining has been developed, which additionally enables selective mapping of cellular structures within the cochlea. Thus, we present results of SLOT imaging rodent cochleae showing specific anatomical structures such as hair cells and neurofilament via fluorescence. In conclusion, the presented study has shown that SLOT is an ideally suited tool in the field of otology for in toto visualization of the inner ear microstructure. © 2016 SPIE

Microbial arms race: Ballistic "nematocysts" in dinoflagellates represent a new extreme in organelle complexity

We examine the origin of harpoon-like secretory organelles (nematocysts) in dinoflagellate protists. These ballistic organelles have been hypothesized to be homologous to similarly complex structures in animals (cnidarians); but we show, using structural, functional, and phylogenomic data, that nematocysts evolved independently in both lineages. We also recorded the first high-resolution videos of nematocyst discharge in dinoflagellates. Unexpectedly, our data suggest that different types of dinoflagellate nematocysts use two fundamentally different types of ballistic mechanisms: one type relies on a single pressurized capsule for propulsion, whereas the other type launches 11 to 15 projectiles froman arrangement similar to a Gatling gun.Despite their radical structural differences, these nematocysts share a single origin within dinoflagellates and both potentially use a contraction-based mechanism to generate ballistic force. The diversity of traits in dinoflagellate nematocysts demonstrates a stepwise route by which simple secretory structures diversified to yield elaborate subcellular weaponry

Scanning laser optical tomography for in toto imaging of the murine cochlea

The mammalian cochlea is a complex macroscopic structure due to its helical shape and the microscopic arrangements of the individual layers of cells. To improve the outcomes of hearing restoration in deaf patients, it is important to understand the anatomic structure and composition of the cochlea ex vivo. Hitherto, only one histological technique based on confocal laser scanning microscopy and optical clearing has been developed for in toto optical imaging of the murine cochlea. However, with a growing size of the specimen, e.g., human cochlea, this technique reaches its limitations. Here, we demonstrate scanning laser optical tomography (SLOT) as a valuable imaging technique to visualize the murine cochlea in toto without any physical slicing. This technique can also be applied in larger specimens up to cm3 such as the human cochlea. Furthermore, immunolabeling allows visualization of inner hair cells (otoferlin) or spiral ganglion cells (neurofilament) within the whole cochlea. After image reconstruction, the 3D dataset was used for digital segmentation of the labeled region. As a result, quantitative analysis of position, length and curvature of the labeled region was possible. This is of high interest in order to understand the interaction of cochlear implants (CI) and cells in more detail. © 2017 Nolte et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.DFG/EXC/1077/1Ministry of Lower SaxonyVolkswagenStiftun

Highlighting New Possibilities: A Comparison of SLOT and CT in the Analysis of 3D Printed Optical Elements

Novel printing techniques for optical elements produce characteristics in these optical elements which can sometimes not be resolved with classic testing or imaging methods. As highly individualized components often have a significant cost associated with them, destructive methods are unwanted. For this work, Scanning Laser Optical Tomography (SLOT) is considered to overcome these challenges and provide knowledge and data which would otherwise not be accessible. SLOT is directly compared to µCT which is often the go-to imaging modality for small, printed optics. Advantages such as the possibility to measure fluorescence with volumetric resolution are presented in this work in the application of a functionalized silicone waveguide. Interactions between the excitation light and the sample are also measured in SLOT in form of absorption. The importance of absorption is highlighted with the example of a 3D printed glass block which has light refractive layers that are invisible in µCT measurements. SLOT should be considered as a quality control tool by scientists and manufactures of printed optical elements as it has many advantages over µCT

The Chemical Composition and Gas-to-Dust Mass Ratio of Nearby Interstellar Matter

Recent results on nearby interstellar gas and interstellar byproducts within the solar system are used to select among the equilibrium radiative transfer models of the nearest interstellar material of Slavin and Frisch (2002). Assuming O/H~400 ppm, Models 2 and 8 are found to yield good fits to available data on local interstellar material, and pickup ions and anomalous cosmic rays inside of the heliosphere, with the exception of Ne. For these models, the density of interstellar gas at the entry point to the heliosphere is n(HI)~0.20 /cc and n(e-)~0.1 /cc. These models suggest the chemical composition of the nearby ISM is ~60--70% subsolar if S is undepleted (where HI and HII must be included for abundance calculations). Gas-to-dust mass ratios of 178-183 for solar abundances, or 611-657 for 70% solar abundances are found. The percentage of the dust mass that is carried by iron is directly correlated with the gas-to-dust mass ratio implying an Fe-rich grain core remains after grain destruction.Comment: 36 pages, 6 figures, accepted by Ap

The Boundary Conditions of the Heliosphere: Photoionization Models Constrained by Interstellar and In Situ Data

The boundary conditions of the heliosphere are set by the ionization, density and composition of inflowing interstellar matter. Constraining the properties of the Local Interstellar Cloud (LIC) at the heliosphere requires radiative transfer ionization models. We model the background interstellar radiation field using observed stellar FUV and EUV emission and the diffuse soft X-ray background. We also model the emission from the boundary between the LIC and the hot Local Bubble (LB) plasma, assuming that the cloud is evaporating because of thermal conduction. We create a grid of models covering a plausible range of LIC and LB properties, and use the modeled radiation field as input to radiative transfer/thermal equilibrium calculations using the Cloudy code. Data from in situ observations of He^O, pickup ions and anomalous cosmic rays in the heliosphere, and absorption line measurements towards epsilon CMa were used to constrain the input parameters. A restricted range of assumed LIC HI column densities and LB plasma temperatures produce models that match all the observational constraints. The relative weakness of the constraints on N(HI) and T_h contrast with the narrow limits predicted for the H^O and electron density in the LIC at the Sun, n(H^0) = 0.19 - 0.20 cm^-3, and n(e) = 0.07 +/- 0.01 cm^-3. Derived abundances are mostly typical for low density gas, with sub-solar Mg, Si and Fe, possibly subsolar O and N, and S about solar; however C is supersolar. The interstellar gas at the Sun is warm, low density, and partially ionized, with n(H) = 0.23 - 0.27 cm^-3, T = 6300 K, X(H^+) ~ 0.2, and X(He^+) ~ 0.4. These results appear to be robust since acceptable models are found for substantially different input radiation fields. Our results favor low values for the reference solar abundances for the LIC composition.Comment: 14 pages, 4 figures, submitted to Astronomy & Astrophysics together with papers from the International Space Sciences Institute workshop on Interstellar Hydrogen in the Heliospher

The future is distributed: a vision of sustainable economies

“The Future is distributed: a vision of sustainable economies” is a collection of case studies on distributed economies, a concept describing sustainable alternatives to the existing business models. The authors of this publication are international Masters students of the Environmental Sciences, Policy and Management Programme at the International Institute for Industrial Environmental Economics at Lund University in Sweden. The aim of their work is to demonstrate that local, small-scale, community-based economies are not just part of the theory, but have already been implemented in various sectors and geographical settings

First detection of a VHE gamma-ray spectral maximum from a Cosmic source: H.E.S.S. discovery of the Vela X nebula

The Vela supernova remnant (SNR) is a complex region containing a number of sources of non-thermal radiation. The inner section of this SNR, within 2 degrees of the pulsar PSR B0833-45, has been observed by the H.E.S.S. gamma-ray atmospheric Cherenkov detector in 2004 and 2005. A strong signal is seen from an extended region to the south of the pulsar, within an integration region of radius 0.8 deg. around the position (RA = 08h 35m 00s, dec = -45 deg. 36' J2000.0). The excess coincides with a region of hard X-ray emission seen by the ROSAT and ASCA satellites. The observed energy spectrum of the source between 550 GeV and 65 TeV is well fit by a power law function with photon index = 1.45 +/- 0.09(stat) +/- 0.2(sys) and an exponential cutoff at an energy of 13.8 +/- 2.3(stat) +/- 4.1(sys) TeV. The integral flux above 1 TeV is (1.28 +/- 0.17 (stat) +/- 0.38(sys)) x 10^{-11} cm^{-2} s^{-1}. This result is the first clear measurement of a peak in the spectral energy distribution from a VHE gamma-ray source, likely related to inverse Compton emission. A fit of an Inverse Compton model to the H.E.S.S. spectral energy distribution gives a total energy in non-thermal electrons of ~2 x 10^{45} erg between 5 TeV and 100 TeV, assuming a distance of 290 parsec to the pulsar. The best fit electron power law index is 2.0, with a spectral break at 67 TeV.Comment: 5 pages, 4 figures, accepted for publication in Astronomy and Astrophysics letter