Stress-induced Gene Expression Sensing Intracellular Heating Triggered by Magnetic Hyperthermia

It is known that alternating magnetic field applications on eukaryotic cells loaded with single domain iron oxide nanoparticles result in high hyperthermic citotoxicity leading to cell dead. Although magnetic hyperthermia therapy for cancer tumours is being developed under this idea, some in vitro assays have shown controversial results indicating that alternating magnetic field triggers large apoptotic effect without significant culture-temperature increase. In agreement with these observations a huge lowering in nanoparticle specific heating rates, when going from the colloidal suspension to cell endosomes, together with cell death, has been reported. Here, we propose a new methodology to determine the occurrence of local heating in cells when alternating magnetic fields in the radiofrequency field range are applied to cell cultures holding very low iron oxide concentrations, being these concentrations insufficient to produce a global cell-culture temperature increase up to therapeutic values. To this end, human lung adenocarcinoma cells (A549 cell line) were transduced with a lentiviral vector encoding the expression of the enhanced green fluorescence protein, EGFP, under the action of the inducible human heat shock protein 70B promoter. This modified A549 cell line was incubated with aqueous suspensions of magnetite core nanoparticles (uncoated or covered with coating agents like citric acid or silicon oxide), and exposed to radiofrequency fields. The application of an alternating magnetic field to cell cultures loaded with nanoparticles resulted in no global temperature increase but EGFP expression. Stress-inducible gene expression scales with uptake and nanoparticle properties like saturation magnetization and heat dissipation efficiency. Our analysis demonstrates that EGFP expression is linked to a localized intracellular temperature increase.Fil: de Sousa, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física La Plata; ArgentinaFil: Carrea, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Mendoza Zélis, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física La Plata; ArgentinaFil: Muraca, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidade Estadual de Campinas; BrasilFil: Mykhaylyk, Olga. Technische Universitat Munchen; AlemaniaFil: Sosa, Yolanda Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Goya, Rodolfo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Sánchez, Francisco Homero. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física La Plata; ArgentinaFil: Dewey, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Fernández van Raap, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física La Plata; Argentin

Agricultura urbana y periurbana como contribución a la estrategia de conservación de la biodiversidad de Heliconias en la región central de Cuba

New ways of sustainable production on urban and peri- urbans areas have contributed to the conservation of species diversity, as to ornamentals plants that belong to family: Heliconiaceae. For this reason we recorded nine species of Heliconia as new accessions from Central Region of Cuba, that were prospected in gardens and yards of national reference as well as from three botanical gardens in central provinces of Cuba (Cienfuegos, Villa Clara and Santi Spíritus), located in forested mountain areas. Molecular corroboration of the species collected was included, as well as a brief description of the morphological and ecological characteristics of these accessions. We show the high commercial potential they have for the island and the contribution to the territorial expansion of these species. In the propagation strategy we considered the micropropagation through biotechnological techniques of tissue culture and in vitro conservation in Cienfuegos Biofactory. The collaboration of universities and the municipal government of Cuba Central region together with the active participation of the farmers have allowed the development of new areas, gardens and home gardens, despite limited resources

Procedimientos para el monitoreo de la presión intraabdominal. Procedures for monitoring intraabdominal pressure (IAP)

La hipertensión intraabdominal se define como el incremento de la presión dentro de la cavidad abdominal por encima de 10 cm H2O, y se clasifica en cuatro grados de acuerdo a la severidad, I: 10 -15 cm H2O, II: 16 -25 cm H2O, III: 26 -35 cm H2O ó IV: mayor de 35 cm H2O. La mayoría de las alteraciones fisiológicas se dan en los grados III y IV, los efectos fisiológicos de la hipertensión intraabdominal comienzan a darse antes de que el Síndrome de Compartimento Intraabdominal sea clínicamente evidente. La medición de la Presión Intraabdominal (PIA) por vía transvesical es un método sencillo e inocuo, y la determinación de los valores que se corresponden con la aparición de las manifestaciones clínicas del SCA permitirá tomar una conducta quirúrgica precoz y menos riesgosa, que podría mejorar los resultados de la atención a estos pacientes, por lo tanto el objetivo de nuestro trabajo es promover su procedimiento y utilización como criterio de laparotomía. Palabras clave: Presión intraabdominal (PIA), Síndrome compartimental abdominal (SCA). ABSTRACT Intraabdominal hypertension is defined as increased pressure into the abdominal cavity over 10 cm.H2O, and is classified in four degrees in terms of severity: I: 10-15 cm. H2O; II : 16-25 cm. H2O; III : 26-35 cm. H2O; or IV : greater than 35 cm. H2O. Most physiological alterations are frequent in degrees III and IV, the physiological effects of intraabdominal hypertension start before the intraabdominal compartimental Syndrome (ICS) becomes clinically evident. The measurement of the IAP through transvesical route is a simple and harmless method, and the determining of the values corresponding to the emergence of the clinical manifestations of ICS would allow to take an early surgical and less risky stand which could improve the results from the assistance to these patients; therefore, the aim of this work is promoting its procedure and use as criterium of laparotomy. Key words: INTRAABDOMINAL PRESSURE (IAP), INTRAABDOMINAL COMPARTIMENTAL SYNDROME (ICS

Procedimientos para el monitoreo de la presión intraabdominal. Procedures for monitoring intraabdominal pressure (IAP)

La hipertensión intraabdominal se define como el incremento de la presión dentro de la cavidad abdominal por encima de 10 cm H2O, y se clasifica en cuatro grados de acuerdo a la severidad, I: 10 -15 cm H2O, II: 16 -25 cm H2O, III: 26 -35 cm H2O ó IV: mayor de 35 cm H2O. La mayoría de las alteraciones fisiológicas se dan en los grados III y IV, los efectos fisiológicos de la hipertensión intraabdominal comienzan a darse antes de que el Síndrome de Compartimento Intraabdominal sea clínicamente evidente. La medición de la Presión Intraabdominal (PIA) por vía transvesical es un método sencillo e inocuo, y la determinación de los valores que se corresponden con la aparición de las manifestaciones clínicas del SCA permitirá tomar una conducta quirúrgica precoz y menos riesgosa, que podría mejorar los resultados de la atención a estos pacientes, por lo tanto el objetivo de nuestro trabajo es promover su procedimiento y utilización como criterio de laparotomía. Palabras clave: Presión intraabdominal (PIA), Síndrome compartimental abdominal (SCA). ABSTRACT Intraabdominal hypertension is defined as increased pressure into the abdominal cavity over 10 cm.H2O, and is classified in four degrees in terms of severity: I: 10-15 cm. H2O; II : 16-25 cm. H2O; III : 26-35 cm. H2O; or IV : greater than 35 cm. H2O. Most physiological alterations are frequent in degrees III and IV, the physiological effects of intraabdominal hypertension start before the intraabdominal compartimental Syndrome (ICS) becomes clinically evident. The measurement of the IAP through transvesical route is a simple and harmless method, and the determining of the values corresponding to the emergence of the clinical manifestations of ICS would allow to take an early surgical and less risky stand which could improve the results from the assistance to these patients; therefore, the aim of this work is promoting its procedure and use as criterium of laparotomy. Key words: INTRAABDOMINAL PRESSURE (IAP), INTRAABDOMINAL COMPARTIMENTAL SYNDROME (ICS

Stress-induced Gene Expression Sensing Intracellular Heating Triggered by Magnetic Hyperthermia

It is known that alternating magnetic field applications on eukaryotic cells loaded with single domain iron oxide nanoparticles result in high hyperthermic citotoxicity leading to cell dead. Although magnetic hyperthermia therapy for cancer tumours is being developed under this idea, some in vitro assays have shown controversial results indicating that alternating magnetic field triggers large apoptotic effect without significant culture-temperature increase. In agreement with these observations a huge lowering in nanoparticle specific heating rates, when going from the colloidal suspension to cell endosomes, together with cell death, has been reported. Here, we propose a new methodology to determine the occurrence of local heating in cells when alternating magnetic fields in the radiofrequency field range are applied to cell cultures holding very low iron oxide concentrations, being these concentrations insufficient to produce a global cell-culture temperature increase up to therapeutic values. To this end, human lung adenocarcinoma cells (A549 cell line) were transduced with a lentiviral vector encoding the expression of the enhanced green fluorescence protein, EGFP, under the action of the inducible human heat shock protein 70B promoter. This modified A549 cell line was incubated with aqueous suspensions of magnetite core nanoparticles (uncoated or covered with coating agents like citric acid or silicon oxide), and exposed to radiofrequency fields. The application of an alternating magnetic field to cell cultures loaded with nanoparticles resulted in no global temperature increase but EGFP expression. Stress-inducible gene expression scales with uptake and nanoparticle properties like saturation magnetization and heat dissipation efficiency. Our analysis demonstrates that EGFP expression is linked to a localized intracellular temperature increase.Facultad de Ciencias ExactasInstituto de Física La PlataFacultad de Ciencias MédicasInstituto de Investigaciones Bioquímicas de La Plat

Un generador de metadatos openaire conforme con el repositorio nacional de México

Open Science aims to increase access to scientific, technological, educational and innovation works. This task has been possible thanks to digital repositories and their standards-based metadata that facilitate interoperability. In Mexico there is a national repository, made up of institutional repositories, which has established its own metadata scheme based on OpenAIRE. However, it was identified that there was no tool for generating metadata compatible with said repository. This work presents a technological tool that solves the problem, incorporating the catalogs of controlled languages defined by the national repository. The methodology consists of the analysis of the national repository documentation, the creation of templates for metadata and the construction of the user interface in charge of generating the metadata in XML format, which were successfully validated. The benefits ensure greater consistency with what is requested from institutional repositories for the interoperability of their resources.La Ciencia Abierta tiene como objetivo incrementar el acceso a los trabajos científicos, tecnológicos, educativos y de innovación. Esta tarea ha sido posible gracias a los repositorios digitales y sus metadatos basados en estándares que facilitan la interoperabilidad. En México existe el repositorio nacional, compuesto por repositorios institucionales, mismo que ha establecido su propio esquema de metadatos basado en OpenAIRE. Sin embargo, se identificó que no existía una herramienta para la generación de metadatos compatible con dicho repositorio. Este trabajo presenta una herramienta tecnológica que soluciona la problemática, incorporando los catálogos de lenguajes controlados definidos por el repositorio nacional. La metodología consiste en el análisis de la documentación del repositorio nacional, la creación de las plantillas para metadatos y en la construcción de la interfaz del usuario encargada de generar los metadatos en formato XML, los cuales fueron validados exitosamente. Los beneficios aseguran una mayor consistencia con lo solicitado a los repositorios institucionales para la interoperabilidad de sus recursos

Falla cardíaca avanzada en pacientes tratados con dispositivos electrofisiológicos en una unidad de electrofisología, Pereira (Colombia)

Introduction: advanced heart failure it's a disease with a high prevalence in the world wide,with an increasing mortality and hospitalization rates, due to his severity and clinical profile. Which costs to the health system is up to 20% of the resources devoted to the management of cardiovascular disease in North Amerca. A cross-sectional study was made with 70 patients on pharmacological treatment and Cardiac Resynchronization Therapy with or without Implantable Cardioverter (CDI) or Implantable Cardioverter (CDI) among 2010 and 2012.Methods: a cross-sectional study.Results: we found 71% with hypertension, 65,75% ischemic cardiopathy , 58.3% with resynchronizator with or without CDI, 90% had two or more drugs for heart failure, 77% had beta blocker, 90% ACE inhibitor or ARB, 85.7% diuretics.Conclusion: patients with advanced heart failure who underwent electrophysiological therapy, were clinically characterized in Pereira, Colombia.Resumen: la falla cardíaca avanzada es una enfermedad prevalente, con una tasa alta de mortalidad y hospitalización con relación a su estadio y perfil clínico, que cuesta en Norteamérica hasta el 20% de los recursos destinados para el manejo de la enfermedad cardiovascular. Se realizó un análisis transversal a una cohorte de 70 pacientes en tratamiento farmacológico y cardioresincronizador con o sin cardiodesfibirlador, o solo cardiodesfibrilador automático (ICD) implantado entre los años 2010 y 2012.Métodos: el presente es un estudio transversal.Resultados: 71% presentaron hipertensión arterial HTA; 65,75% cardiopatía isquémica, siendo el 58.3% portadores de resincronizador con o sin cardiodesfibrilador. El 90% de los pacientes tiene 2 o más medicamentos, 77% betabloqueador, 90% iECA o ARA II, 85.7% algún diurético.Conclusión: se caracterizaron los pacientes que recibieron terapia electrofisiológica para manejo avanzado de falla cardíaca.ardíaca

Influenza Vaccination for the Prevention of Cardiovascular Disease in the Americas: Consensus document of the Inter-American Society of Cardiology and the Word Heart Federation

Background Cardiovascular mortality is decreasing but remains the leading cause of death world-wide. Respiratory infections such as influenza significantly contribute to morbidity and mortality in patients with cardiovascular disease. Despite of proven benefits, influenza vaccination is not fully implemented, especially in Latin America. Objective The aim was to develop a regional consensus with recommendations regarding influenza vaccination and cardiovascular disease. Methods A multidisciplinary team composed by experts in the management and prevention of cardiovascular disease from the Americas, convened by the Inter-American Society of Cardiology (IASC) and the World Heart Federation (WHF), participated in the process and the formulation of statements. The modified RAND/UCLA methodology was used. This document was supported by a grant from the WHF. Results An extensive literature search was divided into seven questions, and a total of 23 conclusions and 29 recommendations were achieved. There was no disagreement among experts in the conclusions or recommendations. Conclusions There is a strong correlation between influenza and cardiovascular events. Influenza vaccination is not only safe and a proven strategy to reduce cardiovascular events, but it is also cost saving. We found several barriers for its global implementation and potential strategies to overcome them

Influence of taste disorders on dietary behaviors in cancer patients under chemotherapy

<p>Abstract</p> <p>Objectives</p> <p>To determine the relationship between energy and nutrient consumption with chemosensory changes in cancer patients under chemotherapy.</p> <p>Methods</p> <p>We carried out a cross-sectional study, enrolling 60 subjects. Cases were defined as patients with cancer diagnosis after their second chemotherapy cycle (n = 30), and controls were subjects without cancer (n = 30). Subjective changes of taste during treatment were assessed. Food consumption habits were obtained with a food frequency questionnaire validated for Mexican population. Five different concentrations of three basic flavors --sweet (sucrose), bitter (urea), and a novel basic taste, umami (sodium glutamate)-- were used to measure detection thresholds and recognition thresholds (RT). We determine differences between energy and nutrient consumption in cases and controls and their association with taste DT and RT.</p> <p>Results</p> <p>No demographic differences were found between groups. Cases showed higher sweet DT (6.4 vs. 4.4 μmol/ml; p = 0.03) and a higher bitter RT (100 vs. 95 μmol/ml; <it>p </it>= 0.04) than controls. Cases with sweet DT above the median showed significant lower daily energy (2,043 vs.1,586 kcal; p = 0.02), proteins (81.4 vs. 54 g/day; <it>p </it>= 0.01), carbohydrates (246 vs.192 g/day; <it>p </it>= 0.05), and zinc consumption (19 vs.11 mg/day; <it>p </it>= 0.01) compared to cases without sweet DT alteration. Cases with sweet DT and RT above median were associated with lower completion of energy requirements and consequent weight loss. There was no association between flavors DT or RT and nutrient ingestion in the control group.</p> <p>Conclusion</p> <p>Changes of sweet DT and bitter RT in cancer patients under chemotherapy treatment were associated with lower energy and nutrient ingestion. Taste detection and recognition thresholds disorders could be important factors in malnutrition development on patients with cancer under chemotherapy treatment.</p