7 research outputs found
Genome annotation sugarcane cultivar SP80-3280 (Gene functional annotation)
Functional annotation of the high quality gene prediction for sugarcane cultivar SP80-3280. Methods employed BLAST2GO, PlnTFDB, EggNOG, OrthoMC
Genome annotation sugarcane cultivar SP80-3280 (Gene prediction)
Annotation of the draft genome sequence of the polyploid sugarcane cultivar SP80-3280. Evidence for gene prediction were: RNASeq data from Mattiello et al. 2015 (Strand-specific RNASeq short reads from the same cultivar) using BRAKER1, and a de novo transcriptome assembly (Trinity, see Mattiello et al. 2015) from the same data mapped to the genome using PASA, ESTs aligned to the genome using exonerate, and Sorghum bicolor proteins aligned to the genome using Exonerate. Evidences were combined using Evidence Modeller, generating this high quality gene prediction
Advances in Microalgae Biology and Sustainable Applications
It has become more evident that many microalgae respond very differently than land plants to diverse stimuli. Therefore, we cannot reduce microalgae biology to what we have learned from land plants biology. However, we are still at the beginning of a comprehensive understanding of microalgae biology. Microalgae have been posited several times as prime candidates for the development of sustainable energy platforms, making thus the in-depth understanding of their biological features an important objective. Thus, the knowledge related to the basics of microalgae biology must be acquired and shared rapidly, fostering the development of potential applications. Microalgae biology has been studied for more than forty years now and more intensely since the 1970âs, when genetics and molecular biology approaches were integrated into the research programs. Recently, studies on the molecular physiology of microalgae have provided evidences on the particularities of these organisms, mainly in model species, such as Chlamydomonas reinhardtii. Of note, cellular responses in microalgae produce very interesting phenotypes, such as high lipid content in nitrogen deprived cells, increased protein content in cells under high CO2 concentrations, the modification of flagella structure and motility in basal body mutant strains, the different ancient proteins that microalgae uses to dissipate the harmful excess of light energy, the hydrogen production in cells under sulfur deprivation, to mention just a few. Moreover, several research groups are using high-throughput and data-driven technologies, including âomicsâ approaches to investigate microalgae cellular responses at a system-wide level, revealing new features of microalgae biology, highlighting differences between microalgae and land plants. It has been amazing to observe the efforts towards the development and optimization of new technologies required for the proper study of microalgae, including methods that opened new paths to the investigation of important processes such as regulatory mechanisms, signaling crosstalk, chemotactic mechanisms, light responses, chloroplast controlled mechanisms, among others. This is an exciting moment in microalgae research when novel data are been produced and applied by research groups from different areas, such as bioprocesses and biotechnology. Moreover, there has been an increased amount of research groups focused in the study of microalgae as a sustainable source for bioremediation, synthesis of bioproducts and development of bioenergy. Innovative strategies are combining the knowledge of basic sciences on microalgae into their applied processes, resulting in the progression of many applications that hopefully, will achieve the necessary degree of optimization for economically feasible large-scale applications. Advances on the areas of basic microalgae biology and novelties on the essential cellular processes were revealed. Progress in the applied science showed the use of the basic science knowledge into fostering translational research, proposing novel strategies for a sustainable world scenario. In this present e-book, articles presented by research groups from different scientific areas showed, successfully, the increased development of the microalgae research. Herewith, you will find articles ranging from bioprospecting regional microalgae species, through advances in microalgae molecular physiology to the development of techniques for characterization of biomass and the use of biomass into agriculture and bioenergy production. This e-book is an excellent source of knowledge for those working with microalgae basic and applied sciences, and a great opportunity for researchers from both areas to have an overview of the amazing possibilities we have for building an environmentally sustainable future once the knowledge is translated into novel applications
Aspergillus fumigatus protein phosphatase PpzA is involved in iron assimilation, secondary metabolite production, and virulence
Metal restriction imposed by mammalian hosts during an infection is a common mechanism of defence to reduce or avoid the pathogen infection. Metals are essential for organism survival due to its involvement in several biological processes. Aspergillus fumigatus causes invasive aspergillosis, a disease that typically manifests in immunocompromised patients. A. fumigatus PpzA, the catalytic subunit of protein phosphatase Z (PPZ), has been recently identified as associated with iron assimilation. A. fumigatus has 2 highâaffinity mechanisms of iron acquisition during infection: reductive iron assimilation and siderophoreâmediated iron uptake. It has been shown that siderophore production is important for A. fumigatus virulence, differently to the reductive iron uptake system. Transcriptomic and proteomic comparisons between âppzA and wildâtype strains under iron starvation showed that PpzA has a broad influence on genes involved in secondary metabolism. Liquid chromatographyâmass spectrometry under standard and iron starvation conditions confirmed that the ÎppzA mutant had reduced production of pyripyropene A, fumagillin, fumiquinazoline A, triacetylâfusarinine C, and helvolic acid. The ÎppzA was shown to be avirulent in a neutropenic murine model of invasive pulmonary aspergillosis. PpzA plays an important role at the interface between iron starvation, regulation of SM production, and pathogenicity in A. fumigatus
Pan genome of the phytoplankton Emiliania underpins its global distribution
Coccolithophores have influenced the global climate for over 200
million years1. These marine phytoplankton can account for 20 per
cent of total carbon fixation in some systems2. They form blooms
that can occupy hundreds of thousands of square kilometres and
are distinguished by their elegantly sculpted calcium carbonate exoskeletons
(coccoliths), rendering themvisible fromspace3.Although
coccolithophores export carbon in the form of organic matter and
calcite to the sea floor, they also release CO2 in the calcification
process. Hence, they have a complex influence on the carbon cycle,
driving either CO2 production or uptake, sequestration and export
to the deep ocean4. Here we report the first haptophyte reference
genome, from the coccolithophore Emiliania huxleyi strain
CCMP1516, and sequences from 13 additional isolates. Our analyses
reveal a pan genome (core genes plus genes distributed variably
between strains) probably supported by an atypical complement
of repetitive sequence in the genome. Comparisons across strains
demonstrate thatE. huxleyi, which has long been considered a single
species, harbours extensive genome variability reflected in different
metabolic repertoires. Genome variability within this species
complex seems to underpin its capacity both to thrive in habitats
ranging from the equator to the subarctic and to form large-scale
episodic blooms under a wide variety of environmental conditions
Recommended from our members
The compact Selaginella genome identifies changes in gene content associated with the evolution of vascular plants
We report the genome sequence of the nonseed vascular plant, Selaginella moellendorffii, and by comparative genomics identify genes that likely played important roles in the early evolution of vascular plants and their subsequent evolutio