Annotation of the draft genome sequence of the polyploid sugarcane cultivar SP80-3280. Evidence for gene prediction were: RNASeq data from Mattiello et al. 2015 (Strand-specific RNASeq short reads from the same cultivar) using BRAKER1, and a de novo transcriptome assembly (Trinity, see Mattiello et al. 2015) from the same data mapped to the genome using PASA, ESTs aligned to the genome using exonerate, and Sorghum bicolor proteins aligned to the genome using Exonerate. Evidences were combined using Evidence Modeller, generating this high quality gene prediction