494 research outputs found
Regular Spectra and Universal Directionality of Emitted Radiation from a Quadrupolar Deformed Microcavity
We have investigated quasi-eigenmodes of a quadrupolar deformed microcavity
by extensive numerical calculations. The spectral structure is found to be
quite regular, which can be explained on the basis of the fact that the
microcavity is an open system. The far-field emission directions of the modes
show unexpected similarity irrespective of their distinct shapes in phase
space. This universal directionality is ascribed to the influence from the
geometry of the unstable manifolds in the corresponding ray dynamics.Comment: 10 pages 11 figure
An NgAgo tool for genome editing: did CRISPR/Cas9 just find a competitor?
AbstractWhile CRISPR/Cas9-mediated genome editing technology has been experiencing a rapid transformation during the past few years, a recent report on NgAgo-mediated single-stranded DNA-guided genome editing may offer an attractive alternative for genome manipulation. While it's too early to predict whether NgAgo will be able to compete with or be superior to CRISPR/Cas9, the scientific community is anxiously waiting for further optimization and broader applications of the NgAgo genome editing technology
W(h)ither the academy? An exploration of the role of university social work in shaping the future of social work in Europe
A controversial proposal to pilot the training of child protection social workers through an intensive work-based route in England is being supported and funded by the UK Government. Frontline, the brainchild of a former teacher, locates social work training within local authorities (‘the agency’) rather than university social work departments (‘the academy’) and has stimulated debate amongst social work academics about their role in shaping the direction of the profession. As a contribution to this debate, this paper explores the duality of social work education, which derives its knowledge from both the academic social sciences and the experience of practice within social work agencies. While social work education has traditionally been delivered by the academy, this paper also explores whether the delivery of training in the allied professions of probation and nursing by ‘the agency’ is equally effective. Finally, this paper explores the Helsinki model which achieves a synergy of ‘academy’ and ‘agency’. It suggests that there are alternative models of social work education, practice and research which avoid dichotomies between the ‘academy’ and the ‘agency’ and enable the profession to be shaped by both social work academics and practitioners
AKARI and BLAST Observations of the Cassiopeia A Supernova Remnant and Surrounding Interstellar Medium
We use new large area far infrared maps ranging from 65 - 500 microns
obtained with the AKARI and the Balloon-borne Large Aperture Submillimeter
Telescope (BLAST) missions to characterize the dust emission toward the
Cassiopeia A supernova remnant (SNR). Using the AKARI high resolution data we
find a new "tepid" dust grain population at a temperature of ~35K and with an
estimated mass of 0.06 solar masses. This component is confined to the central
area of the SNR and may represent newly-formed dust in the unshocked supernova
ejecta. While the mass of tepid dust that we measure is insufficient by itself
to account for the dust observed at high redshift, it does constitute an
additional dust population to contribute to those previously reported. We fit
our maps at 65, 90, 140, 250, 350, and 500 microns to obtain maps of the column
density and temperature of "cold" dust (near 16 K) distributed throughout the
region. The large column density of cold dust associated with clouds seen in
molecular emission extends continuously from the surrounding interstellar
medium to project on the SNR, where the foreground component of the clouds is
also detectable through optical, X-ray, and molecular extinction. At the
resolution available here, there is no morphological signature to isolate any
cold dust associated only with the SNR from this confusing interstellar
emission. Our fit also recovers the previously detected "hot" dust in the
remnant, with characteristic temperature 100 K.Comment: Accepted for publication in the Astrophysical Journal. Maps and
related data are available at http://blastexperiment.info
The Lick AGN Monitoring Project 2011: Reverberation Mapping of Markarian 50
The Lick AGN Monitoring Project 2011 observing campaign was carried out over
the course of 11 weeks in Spring 2011. Here we present the first results from
this program, a measurement of the broad-line reverberation lag in the Seyfert
1 galaxy Mrk 50. Combining our data with supplemental observations obtained
prior to the start of the main observing campaign, our dataset covers a total
duration of 4.5 months. During this time, Mrk 50 was highly variable,
exhibiting a maximum variability amplitude of a factor of 4 in the U-band
continuum and a factor of 2 in the H-beta line. Using standard
cross-correlation techniques, we find that H-beta and H-gamma lag the V-band
continuum by tau_cen = 10.64(-0.93,+0.82) and 8.43(-1.28,+1.30) days,
respectively, while the lag of He II 4686 is unresolved. The H-beta line
exhibits a symmetric velocity-resolved reverberation signature with shorter
lags in the high-velocity wings than in the line core, consistent with an
origin in a broad-line region dominated by orbital motion rather than infall or
outflow. Assuming a virial normalization factor of f=5.25, the virial estimate
of the black hole mass is (3.2+-0.5)*10^7 solar masses. These observations
demonstrate that Mrk 50 is among the most promising nearby active galaxies for
detailed investigations of broad-line region structure and dynamics.Comment: Accepted for publication in ApJ Letters. 6 pages, 4 figure
High-definition endoscopy with digital chromoendoscopy for histologic prediction of distal colorectal polyps
Background
Distal diminutive colorectal polyps are common and accurate endoscopic prediction of hyperplastic or adenomatous polyp histology could reduce procedural time, costs and potential risks associated with the resection. Within this study we assessed whether digital chromoendoscopy can accurately predict the histology of distal diminutive colorectal polyps according to the ASGE PIVI statement.
Methods
In this prospective cohort study, 224 consecutive patients undergoing screening or surveillance colonoscopy were included. Real time histology of 121 diminutive distal colorectal polyps was evaluated using high-definition endoscopy with digital chromoendoscopy and the accuracy of predicting histology with digital chromoendoscopy was assessed.
Results
The overall accuracy of digital chromoendoscopy for prediction of adenomatous polyp histology was 90.1 %. Sensitivity, specificity, positive and negative predictive values were 93.3, 88.7, 88.7, and 93.2 %, respectively. In high-confidence predictions, the accuracy increased to 96.3 % while sensitivity, specificity, positive and negative predictive values were calculated as 98.1, 94.4, 94.5, and 98.1 %, respectively. Surveillance intervals with digital chromoendoscopy were correctly predicted with >90 % accuracy.
Conclusions
High-definition endoscopy in combination with digital chromoendoscopy allowed real-time in vivo prediction of distal colorectal polyp histology and is accurate enough to leave distal colorectal polyps in place without resection or to resect and discard them without pathologic assessment. This approach has the potential to reduce costs and risks associated with the redundant removal of diminutive colorectal polyps
Escherichia coli challenge and one type of smectite alter intestinal barrier of pigs
An experiment was conducted to determine how an E. coli challenge and dietary clays affect the intestinal barrier of pigs. Two groups of 32 pigs (initial BW: 6.9 ± 1.0 kg) were distributed in a 2 × 4 factorial arrangement of a randomized complete block design (2 challenge treatments: sham or E. coli, and 4 dietary treatments: control, 0.3% smectite A, 0.3% smectite B and 0.3% zeolite), with 8 replicates total. Diarrhea score, growth performance, goblet cell size and number, bacterial translocation from intestinal lumen to lymph nodes, intestinal morphology, and relative amounts of sulfo and sialo mucins were measured. The E. coli challenge reduced performance, increased goblet cell size and number in the ileum, increased bacterial translocation from the intestinal lumen to the lymph nodes, and increased ileal crypt depth. One of the clays (smectite A) tended to increase goblet cell size in ileum, which may indicate enhanced protection. In conclusion, E. coli infection degrades intestinal barrier integrity but smectite A may enhance it
A Massive, Cooling-Flow-Induced Starburst in the Core of a Highly Luminous Galaxy Cluster
In the cores of some galaxy clusters the hot intracluster plasma is dense
enough that it should cool radiatively in the cluster's lifetime, leading to
continuous "cooling flows" of gas sinking towards the cluster center, yet no
such cooling flow has been observed. The low observed star formation rates and
cool gas masses for these "cool core" clusters suggest that much of the cooling
must be offset by astrophysical feedback to prevent the formation of a runaway
cooling flow. Here we report X-ray, optical, and infrared observations of the
galaxy cluster SPT-CLJ2344-4243 at z = 0.596. These observations reveal an
exceptionally luminous (L_2-10 keV = 8.2 x 10^45 erg/s) galaxy cluster which
hosts an extremely strong cooling flow (dM/dt = 3820 +/- 530 Msun/yr). Further,
the central galaxy in this cluster appears to be experiencing a massive
starburst (740 +/- 160 Msun/yr), which suggests that the feedback source
responsible for preventing runaway cooling in nearby cool core clusters may not
yet be fully established in SPT-CLJ2344-4243. This large star formation rate
implies that a significant fraction of the stars in the central galaxy of this
cluster may form via accretion of the intracluster medium, rather than the
current picture of central galaxies assembling entirely via mergers.Comment: 11 pages, 3 figures, 1 table. Supplemental material contains 15
additional pages. Published in Natur
Decontamination of MDA Reagents for Single Cell Whole Genome Amplification
Single cell genomics is a powerful and increasingly popular tool for studying the genetic make-up of uncultured microbes. A key challenge for successful single cell sequencing and analysis is the removal of exogenous DNA from whole genome amplification reagents. We found that UV irradiation of the multiple displacement amplification (MDA) reagents, including the Phi29 polymerase and random hexamer primers, effectively eliminates the amplification of contaminating DNA. The methodology is quick, simple, and highly effective, thus significantly improving whole genome amplification from single cells
Following Mitochondrial Footprints through a Long Mucosal Path to Lung Cancer
BACKGROUND:Mitochondrial DNA (mtDNA) mutations are reported in different tumors. However, there is no information on the temporal development of the mtDNA mutations/content alteration and their extent in normal and abnormal mucosa continuously exposed to tobacco smoke in lung cancer patients. METHODOLOGY:We examined the pattern of mtDNA alteration (mtDNA mutation and content index) in 25 airway mucosal biopsies, corresponding tumors and normal lymph nodes obtained from three patients with primary lung cancers. In addition, we examined the pattern of mtDNA mutation in corresponding tumors and normal lymph nodes obtained from eight other patients with primary lung cancers. The entire 16.5 kb mitochondrial genome was sequenced on Affymetrix Mitochip v2.0 sequencing platform in every sample. To examine mtDNA content index, we performed real-time PCR analysis. PRINCIPAL FINDINGS:The airway mucosal biopsies obtained from three lung cancer patients were histopathologically negative but exhibited multiple clonal mtDNA mutations detectable in the corresponding tumors. One of the patients was operated twice for the removal of tumor from the right upper and left lower lobe respectively within a span of two years. Both of these tumors exhibited twenty identical mtDNA mutations. MtDNA content increased significantly (P<0.001) in the lung cancer and all the histologically negative mucosal biopsies except one compared to the control lymph node. CONCLUSIONS/SIGNIFICANCE:Our results document the extent of massive clonal patches that develop in lifetime smokers and ultimately give rise to clinically significant cancers. These observations shed light on the extent of disease in the airway of smokers traceable through mtDNA mutation. MtDNA mutation could be a reliable tool for molecular assessment of respiratory epithelium exposed to continuous smoke as well as disease detection and monitoring. Functional analysis of the pathogenic mtDNA mutations may be useful to understand their role in lung tumorigenesis
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