COLOR SYMBOLISM IN COSTUMES

Diplomski rad za studij kostimografije Interpretacija kostima kroz simboliku boje, istražuje što je to slojevitost kostima, građom je usmjeren na segment boje te ulazi u interpret autorskih i ne autorskih djela kazališta i filma.This graduate thesis for a master's degree in costume design, explores the question of costume's visual volume and complexity. Color symbolism in costumes and it's body of work is concentrated on the element of color as well as it tries to implement the reasons behind it in a personal work and work of others

Assessment of quality of life of patients with permanent colostomy

Uvod. Uprkos činjenici da je kolostoma intervencija koja se izvodi sa visokim procentom uspešnosti, ona za pacijenta predstavlja narušavanje njegovog fizičkog i psihološkog integriteta, što utiče na predstavu o samom sebi, socijalne i funkcionalne kapacitete i ima veliki uticaj na kvalitet života pacijenta. Ciljevi. Ciljevi istraživanja bili su procena kvaliteta života kod bolesnika sa trajnom kolostomom, procena depresivnosti kod bolesnika sa trajnom kolostomom i procena uticaja depresivnosti na kvalitet života kod bolesnika sa trajnom kolostomom. Metodologija. Istraživanje je bilo dizajnirano po tipu prospektivne studije, sprovedeno u Ambulanti za koloproktologiju Poliklinike Kliničkog centra Vojvodine. U prvoj fazi istraživanja pregledom pacijenata i operacionih lista na Klinici za abdominalnu, endokrinu i transplantacionu hirurgiju Kliničkog centra Vojvodine i broja kolostoma urađenih u periodu između 1.9.2015. godine i 1.8.2016. godine iz operacionih protokola izdvojeni su svi pacijenti kojima je u tom periodu izvedena trajna kolostomija i potom procenjena podobnost za uključivanje u studiju. Ispitanici su anonimno popunjavali upitnik mesec, tri i šest meseci nakon operacije. Instrument se sastojao od nekoliko celina kojima su ispitivani sociodemografski podaci, kvalitet života, skala telesnog izgleda, skala samopoštovanja i Bekova skala depresije. Statistička obrada podataka urađena je u program SPSS for Windows v18. Primenom metoda deskriptivne statistike numeričke kontinuirane varijable prezentovane su kao srednje vrednosti i standardne devijacije (SD) sa rasponom vrednosti, dok su kategorijske varijable prikazane kao apsolutne i relativne vrednosti, odnosno distribucije frekvencija. Za utvrđivanje značajnosti razlika korušćeni su χ2 test za opisne varijable, a za numeričke varijable parametrijski testovi (t-test i jednosmerna analiza varijanse ANOVA) i neparametrijski testovi (Man-Whitney test i Kruskal Wallis test). Za ponovljena merenja (3 meseca i 6 meseci) korišćeni su odgovarajući testovi uparenosti za ponovljena merenja. Rezultati. Vrednosti kvaliteta života imale su statistički značajan porast trećeg i šestog meseca u nakon operativnog izvođenja kolostome u odnosu na anketiranje sprovedeno mesec dana nakon operacije. Skala telesnog izgleda i skala samopoštovanja takođe su imale značajno više vrednosti tri i šest meseci nakon operacije poredeći sa vrednostima mesec dana nakon izvođenja kolostome. Prvog meseca udeo osoba sa depresivnim tegobama iznosio je 57,1%, da bi u narednim fazama istraživanja došlo do značajnog pada (41,1% trećeg i 32,1% šestog meseca). Zaključak. Najlošije vrednosti svih parametara iskazane su mesec dana nakon operacije, ali vremenom dolazi do statističkog porasta vrednosti. Bolesnici koji imaju znake depresivnosti imaju značajno lošiji kvalitet života od bolesnika sa trajnom kolostomom koji nemaju znake depresivnosti.Introduction. Despite the fact that colostomy can be described as an intervention with high success rates, for the patient it represents deterioration of their physical and psychological integrity, which influence the body self image, social and functional capacities and influence the quality of life. Aim. Assessment of quality of life of patients with the permanent colostomy, assessment of depression among patients with the permanent colostomy and assessment of depression influence on quality of life of the patients with the permanent colostomy. Methodology. This was a prospective study performed at the Ambulance for coloproctology at the Polyclinic of Clinical Center of Vojvodina. In the first phase evaluation of patients and medical records of colostomies between 1.9.2015-1.8.2016. at the Clinic for abdominal, endocrine and transplantation surgery in the Clinical Center of Vojvodina was performed. Patients who were selected were assessed for enrolment in the study. Participants anonymously fulfilled the questionnaire a month, three months and six months after the surgery. The study instrument comprised of several parts (sociodemographic data, quality of life assessment, body image scale, self esteem scale and Beck’s depression inventory. Survey data were analysed in SPSS 18.0. Descriptive analysis included the total value expressed in absolute and relative numbers, mean values and standard deviation. The χ2 test, Man-Whitney test, Kruskal Wallis test, t-test and one-way ANOVA were used to test for difference between sub groups. For repeated measuring appropriate tests were performed. Results. Quality of life was statistical significantly improved three and six months after the surgery, comparing to a survey performed one month after colostomy. Body image scale and Self esteem scale also showed significantly higher values three and six months after colostomy, comparing to the first month. A month after the surgery percentage of participants with depressive symptoms was the highest (57.1%), but in the next phases of the survey significant decline was observed (41,1% three and 32,1% six months after the surgery). Conclusion. The lowest parameters were observed a months after the surgery, but they significantly improved three and six months later. Colostomy patients with depressive symptoms had significantly worst quality of life comparing to their peers without depressive symptoms

Determination of bone regeneration potential of murine embryonic stem cells and bone marrow mesenchymal stem cells

U ovom istraživanju analiziran je koštani regeneracijski potencijal mezenhimnih matičnih stanica (MMS) koštane srži i embrionalnih matičnih stanica (EMS). U tu je svrhu upotrijebljen transgenični mišji model αSMARFP/Col2.3GFP. MMS su identificirane kroz izražaj crvene fluorescentne bjelančevine - RFP (od engl. red fluorescent protein) koja je pod kontrolom promotora za α-aktin glatkih mišića (αSMA). Kako bi se pratila diferencijacija MMS-a i EMS-a u zrele osteoblaste, upotrijebljena je zelena fluorescentna bjelačevina - GFP (od eng. green fluorescent protein), čiji je izražaj pod kontrolom 2,3 kb fragmenta štakorskog promotora za kolagen tip I (Col 2.3). Za obje vrste stanica napravljena je analiza osteogenog potencijala in vitro i analiza in vivo za EMS. Nakon poticanja koštane diferencijacije u uvjetima in vitro, MMS koštane srži koje izražavaju biljeg αSMARFP diferenciraju se u Col2.3GFP+ stanice, tj. u zrele osteoblaste, pokazuju sposobnost mineralizacije i u znatnoj mjeri izražavaju tipične biljege zrelih osteoblasta (osteokalcin i koštanu sijalobjelančevinu). EMS pokazuju slabu aktivnost biljega Col2.3GFP nakon poticanja koštane diferencijacije u uvjetima in vitro. Također pokazuju slab porast koštanih diferencijacijskih biljega, koji bi potvrdili diferencijaciju EMS-a u zrele osteoblaste. Primijećena je znatna aktivnost alkalne fosfataze i prisutnost mineraliziranog matriksa. Rezultati pokazuju da je uspješnost koštane diferencijacije EMS-a in vitro znatno manja u odnosu na koštanu diferencijaciju MMS-a koštane srži, koristeći se standardnim protokolima za koštanu diferencijaciju matičnih stanica. S druge strane, u uvjetima in vivo koristeći se modelom stvaranja teratoma, EMS pokazuju sposobnost diferencijacije u zrele osteoblaste koji izražavaju biljeg Col2.3GFP. Genski konstrukt Col2.3GFP može se upotrebljavati kako bi se potvrdila diferencijacija EMS-a u zrele osteoblaste.Introduction Bone marrow mesenchymal stem cells (MSCs) have been used in regenerative bone biology for more than a decade, as they can be easily recovered from patients. More recently some of the focus in this field has shifted towards the use of embryonic stem cells (ESCs). Previous studies reported that ESCs can be induced to differentiate into cells showing a mature osteoblastic phenotype by culturing them under osteoinductive conditions. This study proposes determination of the bone regeneration potential of murine bone marrow MSCs and ESCs. Materials and methods For this purpose, a murine model (dual transgenic mice αSMARFP/Col2.3GFP) has been utilized. After isolation of cells from the bone marrow of long bones, MSCs have been identified through the expression of alpha-smooth muscle actin (αSMA) promoter directed RFP. To track the transition of MSCs into mature osteoblasts, a bone-specific fragment of rat type I collagen promoter driving green fluorescent protein (Col2.3GFP) has been utilized. In addition, ESC lines have been derived from the same αSMARFP/Col2.3GFP transgenic mice, allowing identification of cells at the mesenchymal stage (αSMARFP) and at mature osteoblast stage (Col2.3GFP). In vitro analysis of osteogenic potential has been made for both types of cells and in vivo analysis (teratoma assay) of osteogenic potential for ESCs. Results Cultures of bone marrow MSCs were established, and after one week a population of αSMARFP expressing cells was noticed. Following cell sorting, replated αSMARFP+ cells (20-30%) were induced to osteogenesis, and strong expression of osteoblast specific transgene Col2.3GFP was noticed, confirming osteogenic ability of αSMARFP+ cells. Osteogenic differentiation was confirmed by detection of mineralization with von Kossa method and by up-regulation of markers of mature osteoblast lineage cells; osteocalcin and bone sialoprotein. Following osteogenic induction in ESCs, expression of alkaline phosphatase and subsequent mineralization (detected by von Kossa staining) was observed. After one week of osteogenic induction, ESCs began to express αSMARFP. This expression was localized to the peripheral area encircling a typical ESC colony. Nevertheless, these αSMARFP+ cells did not show activation of the Col2.3GFP, even after 6 weeks of osteogenic differentiation in vitro. Also, small increases in expression in some of the bone markers analyzed (Colagen type I, bone sialoprotein) were noticed, but most of them appeared minimal compared to the levels expressed by the MSCs, even after six weeks of osteogenic induction. In contrast, in vivo teratoma assay by ECSs showed bone formation and strong Col2.3GFP signal in the areas that stain positive for mineralization by von Kossa. Conclusion These results show that MSCs compare to ESCs, have much more capability to differentiate into mature osteoblast, express specific bone markers and Col2.3GFP transgene after osteogenic induction in vitro. On the other hand, in the functional in vivo assay ESCs show capability to form bone and activate Col2.3GFP marker. The results obtained indicate that detection of alkaline phosphatase activity and mineralization of ESCs cultured under osteogenic conditions is not sufficient to demonstrate osteogenic maturation. This study indicates the utility of the promoter-visual transgene approach to assess the commitment and differentiation of stem cells into the osteoblast lineage. To current knowledge, this study is the first to utilize the expression of Col2.3GFP transgene in the ESCs derived from transgenic mice

Factorisation in Z [x]

Tema ovog rada je faktorizacija polinoma u Z[x]. Polinomi su matematički izrazi koji sadrže varijable i konstante povezane računskim operacijama zbrajanja, oduzimanja i množenja, te nenegativnih cijelobrojnih potencija. Pokazat ćemo da postupak faktorizacije u Q[x] polinoma iz Z[x] završava u konačno mnogo koraka, kao i dokaz da je polinom ireducibilan u Q[x]. Iznijet ćemo dvije ideje koje će nam pomoći učiniti postupak faktorizacije polinoma izvedivijim. Najprije ćemo uvesti pojam granične vrijednosti za koeficijente koji su djelitelji danog polinoma kojeg želimo faktorizirati. U postupcima traženja graničnih vrijednosti za korijene i koeficijente koristit ćemo metodu bisekcije i Newtonovu metodu. Potom ćemo naći učinkoviti način faktorizacije polinoma modulo M, gdje je M dovoljno velik modul. Pri tom ćemo koristiti Berlekampov algoritam faktorizacije i Henselovu metodu faktorizacije.The theme of this paper is the factorization of polynomials in Z[x]. In mathematics, a polynomial is an expression consisting of variables and coefficients, that involves only the operations of addition, subtraction, multiplication, and non-negative integer exponents of variables. We will show that finding a factorization in Q[x] of a monic polynomial of degree n in Z[x], or showing that polynomial is irreducible in Q[x], takes finitely many steps. We will present two ideas that will make factoring process more feasible. Foremost, we will introduce the concept of bound for the coefficients of polynomials of a given polynomial. Thereat we will use the method of bisection and Newton’s method. Thus, we will find an efficient way to factor a polynomial modulo M for M a suitably large modulus. For that process we will use Berlekamp’s Factoring Algorithm and the Hensel Factorization Method

COLOR SYMBOLISM IN COSTUMES

Diplomski rad za studij kostimografije Interpretacija kostima kroz simboliku boje, istražuje što je to slojevitost kostima, građom je usmjeren na segment boje te ulazi u interpret autorskih i ne autorskih djela kazališta i filma.This graduate thesis for a master's degree in costume design, explores the question of costume's visual volume and complexity. Color symbolism in costumes and it's body of work is concentrated on the element of color as well as it tries to implement the reasons behind it in a personal work and work of others

Determination of bone regeneration potential of murine embryonic stem cells and bone marrow mesenchymal stem cells

U ovom istraživanju analiziran je koštani regeneracijski potencijal mezenhimnih matičnih stanica (MMS) koštane srži i embrionalnih matičnih stanica (EMS). U tu je svrhu upotrijebljen transgenični mišji model αSMARFP/Col2.3GFP. MMS su identificirane kroz izražaj crvene fluorescentne bjelančevine - RFP (od engl. red fluorescent protein) koja je pod kontrolom promotora za α-aktin glatkih mišića (αSMA). Kako bi se pratila diferencijacija MMS-a i EMS-a u zrele osteoblaste, upotrijebljena je zelena fluorescentna bjelačevina - GFP (od eng. green fluorescent protein), čiji je izražaj pod kontrolom 2,3 kb fragmenta štakorskog promotora za kolagen tip I (Col 2.3). Za obje vrste stanica napravljena je analiza osteogenog potencijala in vitro i analiza in vivo za EMS. Nakon poticanja koštane diferencijacije u uvjetima in vitro, MMS koštane srži koje izražavaju biljeg αSMARFP diferenciraju se u Col2.3GFP+ stanice, tj. u zrele osteoblaste, pokazuju sposobnost mineralizacije i u znatnoj mjeri izražavaju tipične biljege zrelih osteoblasta (osteokalcin i koštanu sijalobjelančevinu). EMS pokazuju slabu aktivnost biljega Col2.3GFP nakon poticanja koštane diferencijacije u uvjetima in vitro. Također pokazuju slab porast koštanih diferencijacijskih biljega, koji bi potvrdili diferencijaciju EMS-a u zrele osteoblaste. Primijećena je znatna aktivnost alkalne fosfataze i prisutnost mineraliziranog matriksa. Rezultati pokazuju da je uspješnost koštane diferencijacije EMS-a in vitro znatno manja u odnosu na koštanu diferencijaciju MMS-a koštane srži, koristeći se standardnim protokolima za koštanu diferencijaciju matičnih stanica. S druge strane, u uvjetima in vivo koristeći se modelom stvaranja teratoma, EMS pokazuju sposobnost diferencijacije u zrele osteoblaste koji izražavaju biljeg Col2.3GFP. Genski konstrukt Col2.3GFP može se upotrebljavati kako bi se potvrdila diferencijacija EMS-a u zrele osteoblaste.Introduction Bone marrow mesenchymal stem cells (MSCs) have been used in regenerative bone biology for more than a decade, as they can be easily recovered from patients. More recently some of the focus in this field has shifted towards the use of embryonic stem cells (ESCs). Previous studies reported that ESCs can be induced to differentiate into cells showing a mature osteoblastic phenotype by culturing them under osteoinductive conditions. This study proposes determination of the bone regeneration potential of murine bone marrow MSCs and ESCs. Materials and methods For this purpose, a murine model (dual transgenic mice αSMARFP/Col2.3GFP) has been utilized. After isolation of cells from the bone marrow of long bones, MSCs have been identified through the expression of alpha-smooth muscle actin (αSMA) promoter directed RFP. To track the transition of MSCs into mature osteoblasts, a bone-specific fragment of rat type I collagen promoter driving green fluorescent protein (Col2.3GFP) has been utilized. In addition, ESC lines have been derived from the same αSMARFP/Col2.3GFP transgenic mice, allowing identification of cells at the mesenchymal stage (αSMARFP) and at mature osteoblast stage (Col2.3GFP). In vitro analysis of osteogenic potential has been made for both types of cells and in vivo analysis (teratoma assay) of osteogenic potential for ESCs. Results Cultures of bone marrow MSCs were established, and after one week a population of αSMARFP expressing cells was noticed. Following cell sorting, replated αSMARFP+ cells (20-30%) were induced to osteogenesis, and strong expression of osteoblast specific transgene Col2.3GFP was noticed, confirming osteogenic ability of αSMARFP+ cells. Osteogenic differentiation was confirmed by detection of mineralization with von Kossa method and by up-regulation of markers of mature osteoblast lineage cells; osteocalcin and bone sialoprotein. Following osteogenic induction in ESCs, expression of alkaline phosphatase and subsequent mineralization (detected by von Kossa staining) was observed. After one week of osteogenic induction, ESCs began to express αSMARFP. This expression was localized to the peripheral area encircling a typical ESC colony. Nevertheless, these αSMARFP+ cells did not show activation of the Col2.3GFP, even after 6 weeks of osteogenic differentiation in vitro. Also, small increases in expression in some of the bone markers analyzed (Colagen type I, bone sialoprotein) were noticed, but most of them appeared minimal compared to the levels expressed by the MSCs, even after six weeks of osteogenic induction. In contrast, in vivo teratoma assay by ECSs showed bone formation and strong Col2.3GFP signal in the areas that stain positive for mineralization by von Kossa. Conclusion These results show that MSCs compare to ESCs, have much more capability to differentiate into mature osteoblast, express specific bone markers and Col2.3GFP transgene after osteogenic induction in vitro. On the other hand, in the functional in vivo assay ESCs show capability to form bone and activate Col2.3GFP marker. The results obtained indicate that detection of alkaline phosphatase activity and mineralization of ESCs cultured under osteogenic conditions is not sufficient to demonstrate osteogenic maturation. This study indicates the utility of the promoter-visual transgene approach to assess the commitment and differentiation of stem cells into the osteoblast lineage. To current knowledge, this study is the first to utilize the expression of Col2.3GFP transgene in the ESCs derived from transgenic mice

COLOR SYMBOLISM IN COSTUMES

Diplomski rad za studij kostimografije Interpretacija kostima kroz simboliku boje, istražuje što je to slojevitost kostima, građom je usmjeren na segment boje te ulazi u interpret autorskih i ne autorskih djela kazališta i filma.This graduate thesis for a master's degree in costume design, explores the question of costume's visual volume and complexity. Color symbolism in costumes and it's body of work is concentrated on the element of color as well as it tries to implement the reasons behind it in a personal work and work of others

COLOR SYMBOLISM IN COSTUMES

Diplomski rad za studij kostimografije Interpretacija kostima kroz simboliku boje, istražuje što je to slojevitost kostima, građom je usmjeren na segment boje te ulazi u interpret autorskih i ne autorskih djela kazališta i filma.This graduate thesis for a master's degree in costume design, explores the question of costume's visual volume and complexity. Color symbolism in costumes and it's body of work is concentrated on the element of color as well as it tries to implement the reasons behind it in a personal work and work of others

Korea uzrokovana arteriovenskom malformacijom: prikaz slučaja i pregled literature

Chorea is a movement disorder that can be caused by a large range of degenerative, vascular, metabolic and toxic disorders in basal ganglia. Arteriovenous malformations are rare vascular malformations the clinical presentation of which depends on the malformation characteristics and localization. Th ey are most commonly presented with intracranial hemorrhage, while focal neurological deficit is the rarest presentation. A case is reported of a 64-year-old female patient presented with hemichorea. Magnetic resonance imaging and digital subtraction angiography revealed the presence of arteriovenous malformation in the right temporal lobe.Korea je poremećaj pokreta koji može biti uzrokovan velikim rasponom degenerativnih, vaskularnih, metaboličkih i toksičnih poremećaja u bazalnim ganglijima. Arteriovenske malformacije su rijetke vaskularne malformacije klinička prezentacija kojih ovisi o karakteristikama i lokalizaciji malformacije. Najčešće se prezentiraju intrakranijskom hemoragijom, a najrjeđe fokalnim neurološkim defi citom. Prikazujemo slučaj 64-godišnje bolesnice koja se prezentirala hemikoreom. Učinjena magnetska rezonanca i digitalna subtrakcijska angiografi ja otkrila je prisutnost arteriovenske malformacije u medijalnom dijelu desnog temporalnog režnja

Modeling of transport processes in the cigarette principle combustion furnace

This paper presents numerical and experimental investigations of complex and interrelated physical and chemical phenomena that occur during combustion of baled soybean residue in the furnace with the cigarette type of combustion. The result of comprehensive research is reactive flow model of biomass combustion inside furnace. Model is described by set of PDE which define momentum, heat and mass transfer processes in porous and fluid system. The main aim of developed CFD model is numerical simulation of combustion process inside the cigarette furnace. It is also used to provide deeper insight in complex processes occurring during biomass combustion. Verification of proposed numerical model was performed through comprehensive experimental tests on the experimental-industrial plant of 1.5 MW boiler for heating the greenhouses in the Agricultural Corporation in Belgrade. The tests included measurement of flow rate and air and flue gas temperature input and output values on the furnace that are taken as the boundary conditions of the developed model. Comparison of the experimental results shows satisfactory agreement with numerical results (the maximum relative deviation of calculation and measurement temperatures are 10-45%), therefore the developed mathematical model could be used to analyse the effects of structural and parametric (fuel composition, power rate, air excess etc.) changes of the facility, from the standpoint of energy efficiency and ecology